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The map label for this gene is Tgt

Identifier: 20093628

GI number: 20093628

Start: 180142

End: 181521

Strand: Direct

Name: Tgt

Synonym: MK0188

Alternate gene names: 20093628

Gene position: 180142-181521 (Clockwise)

Preceding gene: 20093627

Following gene: 20093629

Centisome position: 10.63

GC content: 60.94

Gene sequence:

>1380_bases
TTGAACGTTGCATTCGAAGTTAAGGATCGGGACGTGGCGGGCAGGCTCGGGCGTCTCGAGGTCAATGGCAGGAGACTGAA
GACGCCCGCCCTGTTGCCCGTGGTTAACCCCAACAAACCCACCTTGGATCCACGGGAGATCTCCAAGCTAGGTTTCGACG
GGGTGATCACGAACGCCTACATTATCCGGAAGCACGAGCACCTACGCGAGCAGGCGCTCGAGGAAGGCGTCCATGGGCTC
CTTGGGTTCGACGGGTTCGTGATGACGGATTCCGGCTCGTTCCAGCTGGCGGAGTACGGCGATGTCGAAGTATCCAACGA
AGAGATCGTGCGGTTCCAAGCGAAGATCGGGTCAGATGTGGGCACTATCCTCGACGTGCCGACGCCACCCGACGCTCCGA
GGTCCAGGGTCGAACGTGACCTCGAGACCACACTGAAAAGGGCTCGCGAAGCGGTGGAGCTCGACGAACACCCTCCGTTA
GCTCTCACCGTCCAAGGGTCCACGTACGAGGACCTTCGGAGACTGTGCGCAGAGAAACTGGCGGAGCTGCCGGCGGCGGT
GTATCCCGTGGGAGGCGTCGTACCGCTGCTGGAGGAGTACCGGTTCGTAGACGTGGTACGAGTGGTCCTAGCCGCGAAGT
CCTCGCTGCCTCCACATCGCCCTGTACACCTCTTCGGTTGCGGGCATCCGCTGGCGATTCCACTCGCCGTCGCGATGGGG
TGCGATCTGTTCGACTCCGCATCTTACGCTATCTACGCCAGATCGGACCGGTACATGTCCATCCTAGGTACTCTGAAGTT
AGAGGAGCTGGAGACCTTCCCGTGTTCCTGCCCAGCGTGTACACGACACGATCCGGACGACGTCCGCGAGATGGAGCCGC
GAGAACGTACCCGAGTGTTGGCTACGCATAACCTCTACGAGTTGCGCAGGGTGATAGAGACCACGAGGCAGGCTATAGTG
TCGGGAGAGCTATGGGAGTTGGCCGAATCGGTATGCCGAGCACATCCGCGTGCTTGGGCAGGGATGGTGGAGTTAGCGCG
ACGTGGAGGAGAACTGGAACGATGGTGTCCGGCCGTAAAGCGTAGCGTGTTCGTATGCGATGAGGTCTCGAAGGGACGTC
CCGAGCTACGTCTCTACCGTCGACGGCTCCGGGACAGGTTCGGCGAGCTCTCGGGGCGTAAAGTGGTGAAAGGGATAAGC
AGACCCTACGCAGAGATCGTCGAGTGGTTAGAACCATGGGAGCTGGCGTTCGCCGACGAGTGGTTAGGAGTGGTACCCGG
AGAACTGTCATGGTCGTACCCTTGCCACTGCCTCGTGGAGCCTAGCGGGGATGATGAGGGTGAGGACCGACGGAGGGGTG
AAGAGGGTCGCAGGCGCTGA

Upstream 100 bases:

>100_bases
GCGATACGTCGGGGACAAGGAGGTCGGGATCGACCCCGAAGAACTCGCCGAGAAGCTCCGAGAGAGTTGTGAGAATGTCG
TGGTACGGGTGTGATTATGT

Downstream 100 bases:

>100_bases
GTTCGTCCTTTATAAGGAAGGGGGATCGGAGTTTTACGTGCCGGACCCGGAACGTTACTCTCAGGATGGCCTTCCCACCC
GTGATGCACCGGTAGCGTTC

Product: queuine/archaeosine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MNVAFEVKDRDVAGRLGRLEVNGRRLKTPALLPVVNPNKPTLDPREISKLGFDGVITNAYIIRKHEHLREQALEEGVHGL
LGFDGFVMTDSGSFQLAEYGDVEVSNEEIVRFQAKIGSDVGTILDVPTPPDAPRSRVERDLETTLKRAREAVELDEHPPL
ALTVQGSTYEDLRRLCAEKLAELPAAVYPVGGVVPLLEEYRFVDVVRVVLAAKSSLPPHRPVHLFGCGHPLAIPLAVAMG
CDLFDSASYAIYARSDRYMSILGTLKLEELETFPCSCPACTRHDPDDVREMEPRERTRVLATHNLYELRRVIETTRQAIV
SGELWELAESVCRAHPRAWAGMVELARRGGELERWCPAVKRSVFVCDEVSKGRPELRLYRRRLRDRFGELSGRKVVKGIS
RPYAEIVEWLEPWELAFADEWLGVVPGELSWSYPCHCLVEPSGDDEGEDRRRGEEGRRR

Sequences:

>Translated_459_residues
MNVAFEVKDRDVAGRLGRLEVNGRRLKTPALLPVVNPNKPTLDPREISKLGFDGVITNAYIIRKHEHLREQALEEGVHGL
LGFDGFVMTDSGSFQLAEYGDVEVSNEEIVRFQAKIGSDVGTILDVPTPPDAPRSRVERDLETTLKRAREAVELDEHPPL
ALTVQGSTYEDLRRLCAEKLAELPAAVYPVGGVVPLLEEYRFVDVVRVVLAAKSSLPPHRPVHLFGCGHPLAIPLAVAMG
CDLFDSASYAIYARSDRYMSILGTLKLEELETFPCSCPACTRHDPDDVREMEPRERTRVLATHNLYELRRVIETTRQAIV
SGELWELAESVCRAHPRAWAGMVELARRGGELERWCPAVKRSVFVCDEVSKGRPELRLYRRRLRDRFGELSGRKVVKGIS
RPYAEIVEWLEPWELAFADEWLGVVPGELSWSYPCHCLVEPSGDDEGEDRRRGEEGRRR
>Mature_459_residues
MNVAFEVKDRDVAGRLGRLEVNGRRLKTPALLPVVNPNKPTLDPREISKLGFDGVITNAYIIRKHEHLREQALEEGVHGL
LGFDGFVMTDSGSFQLAEYGDVEVSNEEIVRFQAKIGSDVGTILDVPTPPDAPRSRVERDLETTLKRAREAVELDEHPPL
ALTVQGSTYEDLRRLCAEKLAELPAAVYPVGGVVPLLEEYRFVDVVRVVLAAKSSLPPHRPVHLFGCGHPLAIPLAVAMG
CDLFDSASYAIYARSDRYMSILGTLKLEELETFPCSCPACTRHDPDDVREMEPRERTRVLATHNLYELRRVIETTRQAIV
SGELWELAESVCRAHPRAWAGMVELARRGGELERWCPAVKRSVFVCDEVSKGRPELRLYRRRLRDRFGELSGRKVVKGIS
RPYAEIVEWLEPWELAFADEWLGVVPGELSWSYPCHCLVEPSGDDEGEDRRRGEEGRRR

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeosine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=342, Percent_Identity=24.5614035087719, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1786607, Length=357, Percent_Identity=26.0504201680672, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17544680, Length=365, Percent_Identity=22.7397260273973, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19920510, Length=358, Percent_Identity=24.0223463687151, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ATGT_METKA (Q8TYV3)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613475.1
- ProteinModelPortal:   Q8TYV3
- SMR:   Q8TYV3
- GeneID:   1477491
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0188
- NMPDR:   fig|190192.1.peg.188
- HOGENOM:   HBG512800
- OMA:   IREGRLW
- BioCyc:   MKAN190192:MK0188-MONOMER
- HAMAP:   MF_01634
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 51750; Mature: 51750

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50890 PUA

Important sites: ACT_SITE 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVAFEVKDRDVAGRLGRLEVNGRRLKTPALLPVVNPNKPTLDPREISKLGFDGVITNAY
CCCEEEECCCHHHCCCCEEEECCCEECCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHH
IIRKHEHLREQALEEGVHGLLGFDGFVMTDSGSFQLAEYGDVEVSNEEIVRFQAKIGSDV
EEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCEECCHHHHHHHHHHCCCC
GTILDVPTPPDAPRSRVERDLETTLKRAREAVELDEHPPLALTVQGSTYEDLRRLCAEKL
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHH
AELPAAVYPVGGVVPLLEEYRFVDVVRVVLAAKSSLPPHRPVHLFGCGHPLAIPLAVAMG
HHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHC
CDLFDSASYAIYARSDRYMSILGTLKLEELETFPCSCPACTRHDPDDVREMEPRERTRVL
CCHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHHHH
ATHNLYELRRVIETTRQAIVSGELWELAESVCRAHPRAWAGMVELARRGGELERWCPAVK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHH
RSVFVCDEVSKGRPELRLYRRRLRDRFGELSGRKVVKGISRPYAEIVEWLEPWELAFADE
HHEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCHHHHHHHHH
WLGVVPGELSWSYPCHCLVEPSGDDEGEDRRRGEEGRRR
HHCCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MNVAFEVKDRDVAGRLGRLEVNGRRLKTPALLPVVNPNKPTLDPREISKLGFDGVITNAY
CCCEEEECCCHHHCCCCEEEECCCEECCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHH
IIRKHEHLREQALEEGVHGLLGFDGFVMTDSGSFQLAEYGDVEVSNEEIVRFQAKIGSDV
EEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCEECCHHHHHHHHHHCCCC
GTILDVPTPPDAPRSRVERDLETTLKRAREAVELDEHPPLALTVQGSTYEDLRRLCAEKL
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHH
AELPAAVYPVGGVVPLLEEYRFVDVVRVVLAAKSSLPPHRPVHLFGCGHPLAIPLAVAMG
HHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHC
CDLFDSASYAIYARSDRYMSILGTLKLEELETFPCSCPACTRHDPDDVREMEPRERTRVL
CCHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHHHH
ATHNLYELRRVIETTRQAIVSGELWELAESVCRAHPRAWAGMVELARRGGELERWCPAVK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHH
RSVFVCDEVSKGRPELRLYRRRLRDRFGELSGRKVVKGISRPYAEIVEWLEPWELAFADE
HHEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCHHHHHHHHH
WLGVVPGELSWSYPCHCLVEPSGDDEGEDRRRGEEGRRR
HHCCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00006 {guanine}} 0.000053 {guanine}} 0.000014 {7-(aminomethyl)-7-deazaguanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014