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The map label for this gene is act [H]

Identifier: 20093627

GI number: 20093627

Start: 179476

End: 180135

Strand: Direct

Name: act [H]

Synonym: MK0187

Alternate gene names: 20093627

Gene position: 179476-180135 (Clockwise)

Preceding gene: 20093626

Following gene: 20093628

Centisome position: 10.59

GC content: 62.12

Gene sequence:

>660_bases
CTGCGAATAGCAGGCCTGAGACCCGTGAGCTGCTCCGATGGCTTACCCGGTGAAGTGTGTGCGGTCCTTTGGACTCAAGG
ATGTCCGCTACGATGTCCATGGTGCCACAACCCGGAGACCCGCGATCCGAACGGGGGAAAGAAGGCCGATGTCGAGACCA
TCTTACGGGACGTGGAGAAGTACGCCGTTTACCTGGACGCACTGATAGTGAGCGGAGGAGAACCACTCCTGCAGCCGTGC
GAGGAGCTGAAAGCGCTGGCACGTGGTGCTCGGGGCTTGGGTCTCAAGGTCGTCTTGGATACCTCCGGGTTCCCCCCGGA
TCGCCTCGGGAAGGTCATCTCGAGCTTCGACAGGGTAGCGCTGGACCTGAAAGCGCCTCTACGCGATGACGAGTACATGG
AAGCGACTGGCGGCGGCATGACGGCTTCGGACTTCCTGAAAGCGGCCCGGATAGCACGCCGGCGATGTGACCTGGAACTG
CGGATCACGGTGCATCCGTGGCTTGACGACGTGCCGAGGGTCGTGGAGGCAGTCCGGAAGGCGTCCCCCGACGTGGTGGT
CGTTCAGCGATACGTCGGGGACAAGGAGGTCGGGATCGACCCCGAAGAACTCGCCGAGAAGCTCCGAGAGAGTTGTGAGA
ATGTCGTGGTACGGGTGTGA

Upstream 100 bases:

>100_bases
GTACTTCAGACGGTCGTGGGAGACGTACAAGCGCGTTGAGAGTGAAGTGAAGCAGCGAAAGCTCGAGGACTTCTTCTGAG
CGCGCGGAGGGGGGTTAAGA

Downstream 100 bases:

>100_bases
TTATGTTTGAACGTTGCATTCGAAGTTAAGGATCGGGACGTGGCGGGCAGGCTCGGGCGTCTCGAGGTCAATGGCAGGAG
ACTGAAGACGCCCGCCCTGT

Product: pyruvate-formate lyase-activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MRIAGLRPVSCSDGLPGEVCAVLWTQGCPLRCPWCHNPETRDPNGGKKADVETILRDVEKYAVYLDALIVSGGEPLLQPC
EELKALARGARGLGLKVVLDTSGFPPDRLGKVISSFDRVALDLKAPLRDDEYMEATGGGMTASDFLKAARIARRRCDLEL
RITVHPWLDDVPRVVEAVRKASPDVVVVQRYVGDKEVGIDPEELAEKLRESCENVVVRV

Sequences:

>Translated_219_residues
MRIAGLRPVSCSDGLPGEVCAVLWTQGCPLRCPWCHNPETRDPNGGKKADVETILRDVEKYAVYLDALIVSGGEPLLQPC
EELKALARGARGLGLKVVLDTSGFPPDRLGKVISSFDRVALDLKAPLRDDEYMEATGGGMTASDFLKAARIARRRCDLEL
RITVHPWLDDVPRVVEAVRKASPDVVVVQRYVGDKEVGIDPEELAEKLRESCENVVVRV
>Mature_219_residues
MRIAGLRPVSCSDGLPGEVCAVLWTQGCPLRCPWCHNPETRDPNGGKKADVETILRDVEKYAVYLDALIVSGGEPLLQPC
EELKALARGARGLGLKVVLDTSGFPPDRLGKVISSFDRVALDLKAPLRDDEYMEATGGGMTASDFLKAARIARRRCDLEL
RITVHPWLDDVPRVVEAVRKASPDVVVVQRYVGDKEVGIDPEELAEKLRESCENVVVRV

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=177, Percent_Identity=31.0734463276836, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 24031; Mature: 24031

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAGLRPVSCSDGLPGEVCAVLWTQGCPLRCPWCHNPETRDPNGGKKADVETILRDVEK
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
YAVYLDALIVSGGEPLLQPCEELKALARGARGLGLKVVLDTSGFPPDRLGKVISSFDRVA
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
LDLKAPLRDDEYMEATGGGMTASDFLKAARIARRRCDLELRITVHPWLDDVPRVVEAVRK
HHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHH
ASPDVVVVQRYVGDKEVGIDPEELAEKLRESCENVVVRV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MRIAGLRPVSCSDGLPGEVCAVLWTQGCPLRCPWCHNPETRDPNGGKKADVETILRDVEK
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
YAVYLDALIVSGGEPLLQPCEELKALARGARGLGLKVVLDTSGFPPDRLGKVISSFDRVA
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
LDLKAPLRDDEYMEATGGGMTASDFLKAARIARRRCDLELRITVHPWLDDVPRVVEAVRK
HHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHH
ASPDVVVVQRYVGDKEVGIDPEELAEKLRESCENVVVRV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]