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The map label for this gene is RnhB

Identifier: 20093626

GI number: 20093626

Start: 178822

End: 179454

Strand: Direct

Name: RnhB

Synonym: MK0186

Alternate gene names: 20093626

Gene position: 178822-179454 (Clockwise)

Preceding gene: 20093625

Following gene: 20093627

Centisome position: 10.55

GC content: 58.77

Gene sequence:

>633_bases
ATGAGCGGTGTAATGGGTATCGATGAAGCCGGACGTGGACCCGTGTTCGGCCCTATGGTCGTGGCCGGTGTACTGGCTCC
GAAGCGGGAGCTCGGCCTAGGCGCGAGAGATTCGAAGGAACTCACACGGAGTGCGCGTCGTAGACTGATCCGCGCGTTGA
TGTCGGACGAGCGATTAAGGGTGGACCTGAGGATAGTGTGGCCGTGGGAGATAGACGAAGAGGGCGTGGCGAAGGCGGAG
TTCGAAGCTATCAGGGAGCTAGTACGCCGTGCTATGCCCGACGAGGTAATCCTGGACAAACCGGGAAACTACTCACCCGA
GCGACTGCGAAGGGAGCTCGACTTGCCCGAAGGAATCAACCTAATCGCTGAAGAGAGGGCGGACGCGAAGTACGAAGTAG
TTTCAGCGGCCTCGATCGTAGCCAAGACGTACCGTGACTGGATAGTAAGGCTCCTGGAGCTGGAATACGGAGAGGTCGGG
TCGGGGTACCCTTCGGACCCCCGTACCGTAGATCGCCTTCGTCGGGAACTACGTCGGGGTGGTGAGTTGCTGAAGTACTT
CAGACGGTCGTGGGAGACGTACAAGCGCGTTGAGAGTGAAGTGAAGCAGCGAAAGCTCGAGGACTTCTTCTGA

Upstream 100 bases:

>100_bases
CTCCTCCAGTTCCTCCTTCCGGGCGTACTTAGCGAGCACGTCGAACGCTTCCTCCAACCCTCTTCCACCCCCGCTGGAGC
GAAGCTTAGGGGTTCAAAAG

Downstream 100 bases:

>100_bases
GCGCGCGGAGGGGGGTTAAGACTGCGAATAGCAGGCCTGAGACCCGTGAGCTGCTCCGATGGCTTACCCGGTGAAGTGTG
TGCGGTCCTTTGGACTCAAG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSGVMGIDEAGRGPVFGPMVVAGVLAPKRELGLGARDSKELTRSARRRLIRALMSDERLRVDLRIVWPWEIDEEGVAKAE
FEAIRELVRRAMPDEVILDKPGNYSPERLRRELDLPEGINLIAEERADAKYEVVSAASIVAKTYRDWIVRLLELEYGEVG
SGYPSDPRTVDRLRRELRRGGELLKYFRRSWETYKRVESEVKQRKLEDFF

Sequences:

>Translated_210_residues
MSGVMGIDEAGRGPVFGPMVVAGVLAPKRELGLGARDSKELTRSARRRLIRALMSDERLRVDLRIVWPWEIDEEGVAKAE
FEAIRELVRRAMPDEVILDKPGNYSPERLRRELDLPEGINLIAEERADAKYEVVSAASIVAKTYRDWIVRLLELEYGEVG
SGYPSDPRTVDRLRRELRRGGELLKYFRRSWETYKRVESEVKQRKLEDFF
>Mature_209_residues
SGVMGIDEAGRGPVFGPMVVAGVLAPKRELGLGARDSKELTRSARRRLIRALMSDERLRVDLRIVWPWEIDEEGVAKAEF
EAIRELVRRAMPDEVILDKPGNYSPERLRRELDLPEGINLIAEERADAKYEVVSAASIVAKTYRDWIVRLLELEYGEVGS
GYPSDPRTVDRLRRELRRGGELLKYFRRSWETYKRVESEVKQRKLEDFF

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Homo sapiens, GI38455391, Length=217, Percent_Identity=31.3364055299539, Blast_Score=88, Evalue=6e-18,
Organism=Escherichia coli, GI1786380, Length=200, Percent_Identity=32, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17536263, Length=221, Percent_Identity=32.579185520362, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324257, Length=237, Percent_Identity=31.6455696202532, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_METKA (Q8TYV5)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613473.1
- ProteinModelPortal:   Q8TYV5
- SMR:   Q8TYV5
- GeneID:   1477489
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0186
- NMPDR:   fig|190192.1.peg.186
- HOGENOM:   HBG584843
- OMA:   YYVDSPD
- BioCyc:   MKAN190192:MK0186-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_A
- InterPro:   IPR004649
- InterPro:   IPR001352
- InterPro:   IPR020787
- InterPro:   IPR023160
- InterPro:   IPR012337
- Gene3D:   G3DSA:1.10.10.460
- PANTHER:   PTHR10954
- TIGRFAMs:   TIGR00729

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 24263; Mature: 24132

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGVMGIDEAGRGPVFGPMVVAGVLAPKRELGLGARDSKELTRSARRRLIRALMSDERLR
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
VDLRIVWPWEIDEEGVAKAEFEAIRELVRRAMPDEVILDKPGNYSPERLRRELDLPEGIN
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCCH
LIAEERADAKYEVVSAASIVAKTYRDWIVRLLELEYGEVGSGYPSDPRTVDRLRRELRRG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
GELLKYFRRSWETYKRVESEVKQRKLEDFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGVMGIDEAGRGPVFGPMVVAGVLAPKRELGLGARDSKELTRSARRRLIRALMSDERLR
CCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
VDLRIVWPWEIDEEGVAKAEFEAIRELVRRAMPDEVILDKPGNYSPERLRRELDLPEGIN
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCCH
LIAEERADAKYEVVSAASIVAKTYRDWIVRLLELEYGEVGSGYPSDPRTVDRLRRELRRG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
GELLKYFRRSWETYKRVESEVKQRKLEDFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014