Click here to switch to the map view.

The map label for this gene is HisF

Identifier: 20093613

GI number: 20093613

Start: 169255

End: 170073

Strand: Reverse

Name: HisF

Synonym: MK0173

Alternate gene names: 20093613

Gene position: 170073-169255 (Counterclockwise)

Preceding gene: 20093618

Following gene: 20093612

Centisome position: 10.03

GC content: 59.83

Gene sequence:

>819_bases
ATGGCCTTAGCCAAACGCATCATACCCTGCCTCGACGTCAAGGACGGACGTGTCGTGAAAGGCGTACGGTTCCGCGGACT
GCGCGACGCTGGCGATCCCGCTGAGTTGGCCCATCACTACTACCGTCACGGTGCCGACGAGATCGTGTTCCTGGATATCT
CGGCCTCTCCTGAAGGTCGCCGGTTAATGGTGGACGTGGTGCGTCGCACCGCGGAGAAGGTGTTCATTCCCATGACCGTA
GGTGGCGGGATATCCGACGTCGAGGACTTCCGAAGGGCGCTGACCGCCGGAGCCGATAAAGTTTCCGTGAACACCGCGGC
GGTGGAGAACCCGGAGTTAATCTCCGAGGCCGCCGATATTTTCGGGTCGCAGTGCGTGGTAGTGGCGATCGACGCAAAGC
GGGAGCCTCTGAAGCCGGAGCATGAACACGTGGCGGATCATATCTTCTCGAACGACGATGGAGAATACTGGTTCCGGGTC
TACGTCCGTGGAGGTCGTGAGCCCGTAGATCTCGACGCCATAACCTGGGCTAAGAGGGTGGAGGAGCTGGGCGCGGGCGA
GATACTCTTGACGAGCATCGACGCCGACGGTACTCAAGAAGGTTACGACATCGAACTGACCCGAGAGGTGTGTAACGCAG
TGAGCATTCCCGTGATAGCCTCCGGGGGTTGCGGTCACCCGAAGCATATGGTGGAGGTTTTCAAGGAGGCCGACGCCGAC
GCAGCTCTCGCGGCTTCGATCTTCCACTATGGGAAATTCACGATCGAAGAGGTGAAGGAGCACCTGGCCGAAAGGGGTGT
TCGGGTCAGACAGTGCTGA

Upstream 100 bases:

>100_bases
GAAGGTCCCTGGCCACCATCCCGAATGCCACCCCGTCGATGACGATGCTACCCCACGCCGACCCCGATGAGGAGGAACCG
GGGTGTGGGCGGAGACTCCA

Downstream 100 bases:

>100_bases
GAGTCCTGCTCATATATCCCCGATCGCTCCGGTACTTCGTGATCCGAGCGTATCGTGAGCTCCCGTCGACGGTTAAGCCA
GATTCTCCGGTGCTCCTGGC

Product: imidazoleglycerol-phosphate synthase

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MALAKRIIPCLDVKDGRVVKGVRFRGLRDAGDPAELAHHYYRHGADEIVFLDISASPEGRRLMVDVVRRTAEKVFIPMTV
GGGISDVEDFRRALTAGADKVSVNTAAVENPELISEAADIFGSQCVVVAIDAKREPLKPEHEHVADHIFSNDDGEYWFRV
YVRGGREPVDLDAITWAKRVEELGAGEILLTSIDADGTQEGYDIELTREVCNAVSIPVIASGGCGHPKHMVEVFKEADAD
AALAASIFHYGKFTIEEVKEHLAERGVRVRQC

Sequences:

>Translated_272_residues
MALAKRIIPCLDVKDGRVVKGVRFRGLRDAGDPAELAHHYYRHGADEIVFLDISASPEGRRLMVDVVRRTAEKVFIPMTV
GGGISDVEDFRRALTAGADKVSVNTAAVENPELISEAADIFGSQCVVVAIDAKREPLKPEHEHVADHIFSNDDGEYWFRV
YVRGGREPVDLDAITWAKRVEELGAGEILLTSIDADGTQEGYDIELTREVCNAVSIPVIASGGCGHPKHMVEVFKEADAD
AALAASIFHYGKFTIEEVKEHLAERGVRVRQC
>Mature_271_residues
ALAKRIIPCLDVKDGRVVKGVRFRGLRDAGDPAELAHHYYRHGADEIVFLDISASPEGRRLMVDVVRRTAEKVFIPMTVG
GGISDVEDFRRALTAGADKVSVNTAAVENPELISEAADIFGSQCVVVAIDAKREPLKPEHEHVADHIFSNDDGEYWFRVY
VRGGREPVDLDAITWAKRVEELGAGEILLTSIDADGTQEGYDIELTREVCNAVSIPVIASGGCGHPKHMVEVFKEADADA
ALAASIFHYGKFTIEEVKEHLAERGVRVRQC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=270, Percent_Identity=43.7037037037037, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87082028, Length=258, Percent_Identity=25.968992248062, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=38.5350318471338, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_METKA (Q8TYW8)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613460.1
- ProteinModelPortal:   Q8TYW8
- SMR:   Q8TYW8
- GeneID:   1477476
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0173
- NMPDR:   fig|190192.1.peg.173
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- BioCyc:   MKAN190192:MK0173-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 29848; Mature: 29717

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAKRIIPCLDVKDGRVVKGVRFRGLRDAGDPAELAHHYYRHGADEIVFLDISASPEGR
CCCHHHCCCCCCCCCCCEEECEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCC
RLMVDVVRRTAEKVFIPMTVGGGISDVEDFRRALTAGADKVSVNTAAVENPELISEAADI
CHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCEEEECHHCCCCHHHHHHHHHH
FGSQCVVVAIDAKREPLKPEHEHVADHIFSNDDGEYWFRVYVRGGREPVDLDAITWAKRV
CCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHH
EELGAGEILLTSIDADGTQEGYDIELTREVCNAVSIPVIASGGCGHPKHMVEVFKEADAD
HHCCCCCEEEEEECCCCCCCCCCEEHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCH
AALAASIFHYGKFTIEEVKEHLAERGVRVRQC
HHHHHHHHHHCCEEHHHHHHHHHHCCCEECCC
>Mature Secondary Structure 
ALAKRIIPCLDVKDGRVVKGVRFRGLRDAGDPAELAHHYYRHGADEIVFLDISASPEGR
CCHHHCCCCCCCCCCCEEECEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCC
RLMVDVVRRTAEKVFIPMTVGGGISDVEDFRRALTAGADKVSVNTAAVENPELISEAADI
CHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCEEEECHHCCCCHHHHHHHHHH
FGSQCVVVAIDAKREPLKPEHEHVADHIFSNDDGEYWFRVYVRGGREPVDLDAITWAKRV
CCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHH
EELGAGEILLTSIDADGTQEGYDIELTREVCNAVSIPVIASGGCGHPKHMVEVFKEADAD
HHCCCCCEEEEEECCCCCCCCCCEEHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCH
AALAASIFHYGKFTIEEVKEHLAERGVRVRQC
HHHHHHHHHHCCEEHHHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014