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The map label for this gene is Ada

Identifier: 20093570

GI number: 20093570

Start: 125187

End: 125582

Strand: Direct

Name: Ada

Synonym: MK0130

Alternate gene names: 20093570

Gene position: 125187-125582 (Clockwise)

Preceding gene: 20093566

Following gene: 20093571

Centisome position: 7.39

GC content: 62.12

Gene sequence:

>396_bases
GTGCTGGAGGTTACCTTACCGGGAGAGGGCACGCCACCGGCGGAGGGATTCCGCACGCTCCACTCCCTTAAACGTGACCT
GGAGCGGTACTTCCGAGGCTACCCCGTAGACTTCGATGACGTCCCGGTGAGGATAAACGTTTCCGGAAAACCCCGGGAAG
TGCTCGAGCTCGTCCGTGAGATCCCGTACGGGACGGTGGTCACCTACGGGGATATCGCCCAAAAGGCCAACACCCATCCC
CGGGTGGTAGGAGTCTCCCTCGCCCGAAACAGGGTCCCGATAATCGTGGCATGTCACCGCGTCGTAGCCGCCGACGGGCT
CGGTGGGTTCAGGTGGGGGTTGGAATGGAAGAGGAGGCTGTTAGAGCTGGAGGGAGCGTTACCATCGAGGAGGTAG

Upstream 100 bases:

>100_bases
TTCTCGCACAGGCTCGGGCAGGGGGGTCACCCCCTCGCGGTACCGGTTCGCTTATCGGACGCCCTACGGACGCGGGTACG
TAGTCCACGACGGCGAGGCC

Downstream 100 bases:

>100_bases
GCCAGTGGATAGTGATCGTCGACCGTGAGGAGCTCGAGTACGACGGCTCGCAGCTCCGCAGGGCGTTCGCTCACGAGCGG
TACGGAATTAAAGGGCGGGC

Product: methylated DNA-protein cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Number of amino acids: Translated: 131; Mature: 131

Protein sequence:

>131_residues
MLEVTLPGEGTPPAEGFRTLHSLKRDLERYFRGYPVDFDDVPVRINVSGKPREVLELVREIPYGTVVTYGDIAQKANTHP
RVVGVSLARNRVPIIVACHRVVAADGLGGFRWGLEWKRRLLELEGALPSRR

Sequences:

>Translated_131_residues
MLEVTLPGEGTPPAEGFRTLHSLKRDLERYFRGYPVDFDDVPVRINVSGKPREVLELVREIPYGTVVTYGDIAQKANTHP
RVVGVSLARNRVPIIVACHRVVAADGLGGFRWGLEWKRRLLELEGALPSRR
>Mature_131_residues
MLEVTLPGEGTPPAEGFRTLHSLKRDLERYFRGYPVDFDDVPVRINVSGKPREVLELVREIPYGTVVTYGDIAQKANTHP
RVVGVSLARNRVPIIVACHRVVAADGLGGFRWGLEWKRRLLELEGALPSRR

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family

Homologues:

Organism=Caenorhabditis elegans, GI115533070, Length=77, Percent_Identity=42.8571428571429, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115533068, Length=77, Percent_Identity=42.8571428571429, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OGT_METKA (Q8TZ11)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613417.1
- ProteinModelPortal:   Q8TZ11
- SMR:   Q8TZ11
- GeneID:   1477433
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0130
- NMPDR:   fig|190192.1.peg.130
- HOGENOM:   HBG752398
- OMA:   AQKANTH
- BioCyc:   MKAN190192:MK0130-MONOMER
- BRENDA:   2.1.1.63
- GO:   GO:0005737
- HAMAP:   MF_00772
- InterPro:   IPR014048
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10815
- TIGRFAMs:   TIGR00589

Pfam domain/function: PF01035 DNA_binding_1; SSF46767 MethylDNA_cys_mtrans_DNA_bd

EC number: =2.1.1.63

Molecular weight: Translated: 14714; Mature: 14714

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00374 MGMT

Important sites: ACT_SITE 98-98

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEVTLPGEGTPPAEGFRTLHSLKRDLERYFRGYPVDFDDVPVRINVSGKPREVLELVRE
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHH
IPYGTVVTYGDIAQKANTHPRVVGVSLARNRVPIIVACHRVVAADGLGGFRWGLEWKRRL
CCCCCEEEEHHHHHHCCCCCEEEEEEHHCCCCCEEEEHHHHHHHCCCCCHHHHHHHHHHH
LELEGALPSRR
HHHHCCCCCCC
>Mature Secondary Structure
MLEVTLPGEGTPPAEGFRTLHSLKRDLERYFRGYPVDFDDVPVRINVSGKPREVLELVRE
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHH
IPYGTVVTYGDIAQKANTHPRVVGVSLARNRVPIIVACHRVVAADGLGGFRWGLEWKRRL
CCCCCEEEEHHHHHHCCCCCEEEEEEHHCCCCCEEEEHHHHHHHCCCCCHHHHHHHHHHH
LELEGALPSRR
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014