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The map label for this gene is GatE

Identifier: 20093569

GI number: 20093569

Start: 123173

End: 125095

Strand: Reverse

Name: GatE

Synonym: MK0129

Alternate gene names: 20093569

Gene position: 125095-123173 (Counterclockwise)

Preceding gene: 20093573

Following gene: 20093568

Centisome position: 7.38

GC content: 61.05

Gene sequence:

>1923_bases
GTGCGAGAAGAGGACTTAGACTGGGAAGAGATCGGCCTCCGGGTGGGACTTGAGATCCACCGGCAGCTCGATACTTCCAG
AAAGCTGTTCTGTCGCTGTACTCCAGAGCTGGTGGAAGAGGTACCGAAGGAGCCCAAGGTCAGACGTAAGCTCCGCCCGG
TCCAGAGCGAGATGGGCGAGTTCGATCCGGCGGCCTTGGAGGAGTTCAAGCGCGACCGAACGTTCTACTACCTGGCGGAC
GGGTCCTTCTCGTGCCTAGTCGAACTGGACGAAGAGCCCCCGCACGAGCCTTGTTCTGAAGCCCTTGACGTCGCGATTAA
GGTCACCCTCCTTCTGGGTGGGTCCGTCGTCGACGAGGTGCACGTGATGCGAAAGATGGTGATCGACGGTTCGAACACCA
CCGGCTTCCAGCGTACAATGCTCGTCGGGTTCGGTGGTGAGGTTCCGACGTCCGAAGGCCCCGTCCGTATCAGCACGGTG
TGTCTGGAGGAAGACGCCGCCCGCAAGGTGAAGGGACGTGATCAGGATCTCGAGGTTGATTACTGCCTAGACAGGCTCGG
GATACCGCTTATCGAGGTCTCTACGGAGCCGGACATCAGAACACCGGAACAGGCCCGTGAGGCCGCTGAGAGGATCGGTG
AGGCGATCAAAGCCGTCGGCGGTGTGAAGTCGGGAATCGGTACGGTAAGGCAGGACGTGAACGTCTCGATAGAGGGCGGT
GCCGTGCAGGAGATCAAGGGTGTTCAGGACCTGAACCTGATTCCCAAGGTGGTCAAGTACGAGGCGTTGAGACAGGCGAA
TCTCCTCAGGATCCGGGACGAGCTTCGTGAACGCGGTGTCAGTGAGACGGACCTGATCGACTGTGAGCCCATGGATGTCA
CGGACGTGTTCGAGGACACGGACAGCGAGGTCATCCGTCGGGAGCTCGAGCGCGGTGGTGTAGTGTACGCTCTGCTTCTG
CCCGGGTTCGAGGGGATCTTAGGCTGGGAGCTGTGTCCGGGTCGAAGGTTCGGTACCGAACTCGCCGACTACGCGAGGAG
ACGAGGGGTTTCGGGGCTGTTCCACTCCGATGAGCTCCCCAAGTACGGTATCTCGGAGGAGGAAGTTGAGGCCGTCAGGC
AGCGCCTCGGAGCGGAAGACGGTGACGGGTTCGTCTTGATAGCGGGCCCGGAGGATCGCGTTAAGTCCGCCATGGAAGCG
GTGAAAGATCGTGCCATCATGGCGCTGAAAGGCGTCCCTGCCGAGACCCGCCGCGCTCGGAAGGACGGGACGACGGAGTA
CATGCGGCCCCGCCCGGGAGCCGCTCGAATGTACCCTGAGACGGACATCCCGCCCGTGGTGATCGACGAGGACAGGGTAA
AGGAGCTGGCGGAAGAGCTTCCGGAGAAACCTTGGGAGCGTAAGGAGAGGCTCGCCGAGGAGTACGGTCTCGGGGAGGAG
TTAGTGGAGCAGATGTTCGAGCATGGAGTCGTGGACGAGTTCGAGGAGATTGTGGAGGAGACCGGGGTGGAACCCAAGGT
AGCGGCGGCCACGCTGGTGAACACGATCCCGCGGCTTGAAAAAGACGGGTACCCCGTGGATAACCTCACCATAGACCACG
TTAAGGAAGTGCTGAGGCTTTACGCCGAGGGTGCCATAGCTAAGTCTGGGATCGAGGAGCTGCTGGGTGCCCTGGCGGCC
GATCCGGACTCCGATCCCGAGGAGCTTGCGGAAGAGCTCGGCATCGTGATGGCGTCCGAGGAAGAGATCGAGGAGGTGGT
CGAAGAGGCGATTCGTAGGTACGAGGATAAGATTCGGGAGCGAGGTATGGCAGTCATGGGCAAGATCATGGGTGAGGTGA
TGGAGGTCCTACGGGGGCGGGCGGACGGTAAACGAGTAAGCGAGCTCGTCCGTGAGCGAATTCGAGAGATTTCGGGAGAG
TGA

Upstream 100 bases:

>100_bases
CTCCAGCACGGCCTCGCCGTCGTGGACTACGTACCCGCGTCCGTAGGGCGTCCGATAAGCGAACCGGTACCGCGAGGGGG
TGACCCCCCTGCCCGAGCCT

Downstream 100 bases:

>100_bases
TCAGGTTGGATGTCAGGGAGCGCGTGGTAAAGCTCCTGTGCGACTACATCTCTATCCCCAGCGTCAGCGGCGAGGAGGAA
GAGCTTTCGGAGCGATACGC

Product: glutamyl-tRNA(Gln) amidotransferase subunit E

Products: NA

Alternate protein names: Glu-ADT subunit E

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MREEDLDWEEIGLRVGLEIHRQLDTSRKLFCRCTPELVEEVPKEPKVRRKLRPVQSEMGEFDPAALEEFKRDRTFYYLAD
GSFSCLVELDEEPPHEPCSEALDVAIKVTLLLGGSVVDEVHVMRKMVIDGSNTTGFQRTMLVGFGGEVPTSEGPVRISTV
CLEEDAARKVKGRDQDLEVDYCLDRLGIPLIEVSTEPDIRTPEQAREAAERIGEAIKAVGGVKSGIGTVRQDVNVSIEGG
AVQEIKGVQDLNLIPKVVKYEALRQANLLRIRDELRERGVSETDLIDCEPMDVTDVFEDTDSEVIRRELERGGVVYALLL
PGFEGILGWELCPGRRFGTELADYARRRGVSGLFHSDELPKYGISEEEVEAVRQRLGAEDGDGFVLIAGPEDRVKSAMEA
VKDRAIMALKGVPAETRRARKDGTTEYMRPRPGAARMYPETDIPPVVIDEDRVKELAEELPEKPWERKERLAEEYGLGEE
LVEQMFEHGVVDEFEEIVEETGVEPKVAAATLVNTIPRLEKDGYPVDNLTIDHVKEVLRLYAEGAIAKSGIEELLGALAA
DPDSDPEELAEELGIVMASEEEIEEVVEEAIRRYEDKIRERGMAVMGKIMGEVMEVLRGRADGKRVSELVRERIREISGE

Sequences:

>Translated_640_residues
MREEDLDWEEIGLRVGLEIHRQLDTSRKLFCRCTPELVEEVPKEPKVRRKLRPVQSEMGEFDPAALEEFKRDRTFYYLAD
GSFSCLVELDEEPPHEPCSEALDVAIKVTLLLGGSVVDEVHVMRKMVIDGSNTTGFQRTMLVGFGGEVPTSEGPVRISTV
CLEEDAARKVKGRDQDLEVDYCLDRLGIPLIEVSTEPDIRTPEQAREAAERIGEAIKAVGGVKSGIGTVRQDVNVSIEGG
AVQEIKGVQDLNLIPKVVKYEALRQANLLRIRDELRERGVSETDLIDCEPMDVTDVFEDTDSEVIRRELERGGVVYALLL
PGFEGILGWELCPGRRFGTELADYARRRGVSGLFHSDELPKYGISEEEVEAVRQRLGAEDGDGFVLIAGPEDRVKSAMEA
VKDRAIMALKGVPAETRRARKDGTTEYMRPRPGAARMYPETDIPPVVIDEDRVKELAEELPEKPWERKERLAEEYGLGEE
LVEQMFEHGVVDEFEEIVEETGVEPKVAAATLVNTIPRLEKDGYPVDNLTIDHVKEVLRLYAEGAIAKSGIEELLGALAA
DPDSDPEELAEELGIVMASEEEIEEVVEEAIRRYEDKIRERGMAVMGKIMGEVMEVLRGRADGKRVSELVRERIREISGE
>Mature_640_residues
MREEDLDWEEIGLRVGLEIHRQLDTSRKLFCRCTPELVEEVPKEPKVRRKLRPVQSEMGEFDPAALEEFKRDRTFYYLAD
GSFSCLVELDEEPPHEPCSEALDVAIKVTLLLGGSVVDEVHVMRKMVIDGSNTTGFQRTMLVGFGGEVPTSEGPVRISTV
CLEEDAARKVKGRDQDLEVDYCLDRLGIPLIEVSTEPDIRTPEQAREAAERIGEAIKAVGGVKSGIGTVRQDVNVSIEGG
AVQEIKGVQDLNLIPKVVKYEALRQANLLRIRDELRERGVSETDLIDCEPMDVTDVFEDTDSEVIRRELERGGVVYALLL
PGFEGILGWELCPGRRFGTELADYARRRGVSGLFHSDELPKYGISEEEVEAVRQRLGAEDGDGFVLIAGPEDRVKSAMEA
VKDRAIMALKGVPAETRRARKDGTTEYMRPRPGAARMYPETDIPPVVIDEDRVKELAEELPEKPWERKERLAEEYGLGEE
LVEQMFEHGVVDEFEEIVEETGVEPKVAAATLVNTIPRLEKDGYPVDNLTIDHVKEVLRLYAEGAIAKSGIEELLGALAA
DPDSDPEELAEELGIVMASEEEIEEVVEEAIRRYEDKIRERGMAVMGKIMGEVMEVLRGRADGKRVSELVRERIREISGE

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG2511

COG function: function code J; Archaeal Glu-tRNAGln amidotransferase subunit E (contains GAD domain)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatE subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATE_METKA (Q8TZ12)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613416.1
- ProteinModelPortal:   Q8TZ12
- SMR:   Q8TZ12
- GeneID:   1477432
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0129
- NMPDR:   fig|190192.1.peg.129
- HOGENOM:   HBG297405
- OMA:   TSGFQRT
- ProtClustDB:   PRK04028
- BioCyc:   MKAN190192:MK0129-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00588
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004115
- InterPro:   IPR023168
- InterPro:   IPR004414
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00134

Pfam domain/function: PF02938 GAD; PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 71623; Mature: 71623

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREEDLDWEEIGLRVGLEIHRQLDTSRKLFCRCTPELVEEVPKEPKVRRKLRPVQSEMGE
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCC
FDPAALEEFKRDRTFYYLADGSFSCLVELDEEPPHEPCSEALDVAIKVTLLLGGSVVDEV
CCHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
HVMRKMVIDGSNTTGFQRTMLVGFGGEVPTSEGPVRISTVCLEEDAARKVKGRDQDLEVD
HHHHHHHHCCCCCCCHHHEEEEECCCCCCCCCCCEEEEEEEECHHHHHHHCCCCCCCHHH
YCLDRLGIPLIEVSTEPDIRTPEQAREAAERIGEAIKAVGGVKSGIGTVRQDVNVSIEGG
HHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
AVQEIKGVQDLNLIPKVVKYEALRQANLLRIRDELRERGVSETDLIDCEPMDVTDVFEDT
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCC
DSEVIRRELERGGVVYALLLPGFEGILGWELCPGRRFGTELADYARRRGVSGLFHSDELP
HHHHHHHHHHHCCEEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
KYGISEEEVEAVRQRLGAEDGDGFVLIAGPEDRVKSAMEAVKDRAIMALKGVPAETRRAR
CCCCCHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
KDGTTEYMRPRPGAARMYPETDIPPVVIDEDRVKELAEELPEKPWERKERLAEEYGLGEE
CCCCCHHCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHH
LVEQMFEHGVVDEFEEIVEETGVEPKVAAATLVNTIPRLEKDGYPVDNLTIDHVKEVLRL
HHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
YAEGAIAKSGIEELLGALAADPDSDPEELAEELGIVMASEEEIEEVVEEAIRRYEDKIRE
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCEEEECHHHHHHHHHHHHHHHHHHHHH
RGMAVMGKIMGEVMEVLRGRADGKRVSELVRERIREISGE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MREEDLDWEEIGLRVGLEIHRQLDTSRKLFCRCTPELVEEVPKEPKVRRKLRPVQSEMGE
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCC
FDPAALEEFKRDRTFYYLADGSFSCLVELDEEPPHEPCSEALDVAIKVTLLLGGSVVDEV
CCHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
HVMRKMVIDGSNTTGFQRTMLVGFGGEVPTSEGPVRISTVCLEEDAARKVKGRDQDLEVD
HHHHHHHHCCCCCCCHHHEEEEECCCCCCCCCCCEEEEEEEECHHHHHHHCCCCCCCHHH
YCLDRLGIPLIEVSTEPDIRTPEQAREAAERIGEAIKAVGGVKSGIGTVRQDVNVSIEGG
HHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
AVQEIKGVQDLNLIPKVVKYEALRQANLLRIRDELRERGVSETDLIDCEPMDVTDVFEDT
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCC
DSEVIRRELERGGVVYALLLPGFEGILGWELCPGRRFGTELADYARRRGVSGLFHSDELP
HHHHHHHHHHHCCEEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
KYGISEEEVEAVRQRLGAEDGDGFVLIAGPEDRVKSAMEAVKDRAIMALKGVPAETRRAR
CCCCCHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
KDGTTEYMRPRPGAARMYPETDIPPVVIDEDRVKELAEELPEKPWERKERLAEEYGLGEE
CCCCCHHCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHH
LVEQMFEHGVVDEFEEIVEETGVEPKVAAATLVNTIPRLEKDGYPVDNLTIDHVKEVLRL
HHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
YAEGAIAKSGIEELLGALAADPDSDPEELAEELGIVMASEEEIEEVVEEAIRRYEDKIRE
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCEEEECHHHHHHHHHHHHHHHHHHHHH
RGMAVMGKIMGEVMEVLRGRADGKRVSELVRERIREISGE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014