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The map label for this gene is TruB

Identifier: 20093573

GI number: 20093573

Start: 127591

End: 128607

Strand: Reverse

Name: TruB

Synonym: MK0133

Alternate gene names: 20093573

Gene position: 128607-127591 (Counterclockwise)

Preceding gene: 20093574

Following gene: 20093569

Centisome position: 7.59

GC content: 60.67

Gene sequence:

>1017_bases
ATGAGCGGCGACAAGGACCGGCGGCTGCCGTTCGACCGGGATCGGGAGATGATCACGAAGGCCGAGGTGGAGACGGACCC
ACGGTACGGTTGTCCTCCCGAGGAGCGCCCTATCGAGGAATACATCATGAAGGGTGTCATCAACCTGGACAAGCCGGCCG
GACCCACATCGCACGAGGTTGTGGCTTGGGTGAAGGAGATCTTCGGGCTGTCCAAGGCGGGTCACGGTGGTACCCTGGAC
CCTAAGGTCACGGGAGTCCTTCCGATCGCCCTCGAGAAAGCCACGAAGATAATCCAGACGCTGCTACCGGCGGGTAAGGA
GTACGTGACGATTATGCATCTCCACGGCGATGTGGACGAGGAGGAGCTCGAGCGCGTTGTGAAGGAGTTCGAGGGGACGA
TACTTCAGCGGCCACCGTTACGCTCGGCAGTCAAGAGGAGGGTACGTCCTAAGAAGATCTACTACATCGATATTCTCGAG
ATCGACGGTCGCGACGTGCTGATGAGGGTCGGGTGTCAGGCTGGTACCTACATTCGGAAGCTGTGTCACGACATCGGTGA
GGCCTTGGGTGTCGGTGCGCACATGGCGGAGCTCCGGAGGACGAGGACAGGGCCGTTCTCGGAGGAGAACGCGGTCACGC
TCCACGACGTCAAGGACGCGTACGAGTTCTGGAAGGAGGAGGGATGGGAAGAGCCCTTACGACACGTTGTACGACCGATG
GAGGAAGGTCTCGAGCACCTACCGAGAATAGAGATCAGGGATACGGCCGTCGACGCCATCTGCCACGGAGCCAACCTGGC
TGCGCCGGGAATCGTTAGGGTGGAGAAGGGGATCCAGCCCGGCGATCTAGTCGCCATCTTCACGCTGAAAGGGGAGGCGG
TAGCGCTGGGAGTGGCCAAGGCCACTTGGAAGGAGATGCTACACGCGGATCGGGGTATTATGGTGGACACCAAGAGGGTC
CTGATGGAGCCGGGCACGTACCCGAAGGCTTGGGGGTTAAAGACCCCTGGAGAGTAG

Upstream 100 bases:

>100_bases
CACGCTGTGGAGACCGAGGATCGGAACTTCACGGGGCTGGTGAAGAGGTTATTATCGAGAGCGCCACCAGAGGCGTCGAC
GAACGTTGAGGGGGCCACGC

Downstream 100 bases:

>100_bases
CACCGTTAAGTTCTCCACTCGTGCCTCGACGTCGTTGCGCTCCAGTCCTTCCCTGAGGTTGTATTCGCAGAACGGGCAGA
CCGTGGTCAGCACTTCGGCT

Product: H/ACA RNA-protein complex component Cbf5p

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSGDKDRRLPFDRDREMITKAEVETDPRYGCPPEERPIEEYIMKGVINLDKPAGPTSHEVVAWVKEIFGLSKAGHGGTLD
PKVTGVLPIALEKATKIIQTLLPAGKEYVTIMHLHGDVDEEELERVVKEFEGTILQRPPLRSAVKRRVRPKKIYYIDILE
IDGRDVLMRVGCQAGTYIRKLCHDIGEALGVGAHMAELRRTRTGPFSEENAVTLHDVKDAYEFWKEEGWEEPLRHVVRPM
EEGLEHLPRIEIRDTAVDAICHGANLAAPGIVRVEKGIQPGDLVAIFTLKGEAVALGVAKATWKEMLHADRGIMVDTKRV
LMEPGTYPKAWGLKTPGE

Sequences:

>Translated_338_residues
MSGDKDRRLPFDRDREMITKAEVETDPRYGCPPEERPIEEYIMKGVINLDKPAGPTSHEVVAWVKEIFGLSKAGHGGTLD
PKVTGVLPIALEKATKIIQTLLPAGKEYVTIMHLHGDVDEEELERVVKEFEGTILQRPPLRSAVKRRVRPKKIYYIDILE
IDGRDVLMRVGCQAGTYIRKLCHDIGEALGVGAHMAELRRTRTGPFSEENAVTLHDVKDAYEFWKEEGWEEPLRHVVRPM
EEGLEHLPRIEIRDTAVDAICHGANLAAPGIVRVEKGIQPGDLVAIFTLKGEAVALGVAKATWKEMLHADRGIMVDTKRV
LMEPGTYPKAWGLKTPGE
>Mature_337_residues
SGDKDRRLPFDRDREMITKAEVETDPRYGCPPEERPIEEYIMKGVINLDKPAGPTSHEVVAWVKEIFGLSKAGHGGTLDP
KVTGVLPIALEKATKIIQTLLPAGKEYVTIMHLHGDVDEEELERVVKEFEGTILQRPPLRSAVKRRVRPKKIYYIDILEI
DGRDVLMRVGCQAGTYIRKLCHDIGEALGVGAHMAELRRTRTGPFSEENAVTLHDVKDAYEFWKEEGWEEPLRHVVRPME
EGLEHLPRIEIRDTAVDAICHGANLAAPGIVRVEKGIQPGDLVAIFTLKGEAVALGVAKATWKEMLHADRGIMVDTKRVL
MEPGTYPKAWGLKTPGE

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI4503337, Length=308, Percent_Identity=45.4545454545455, Blast_Score=258, Evalue=5e-69,
Organism=Homo sapiens, GI215599015, Length=308, Percent_Identity=45.4545454545455, Blast_Score=258, Evalue=6e-69,
Organism=Homo sapiens, GI21040257, Length=238, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI2367200, Length=276, Percent_Identity=31.1594202898551, Blast_Score=118, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17553978, Length=308, Percent_Identity=40.2597402597403, Blast_Score=259, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6323204, Length=308, Percent_Identity=45.7792207792208, Blast_Score=286, Evalue=5e-78,
Organism=Drosophila melanogaster, GI281364189, Length=309, Percent_Identity=44.6601941747573, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI281364187, Length=309, Percent_Identity=44.6601941747573, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI281364185, Length=309, Percent_Identity=44.6601941747573, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI281364183, Length=309, Percent_Identity=44.6601941747573, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI62471759, Length=309, Percent_Identity=44.6601941747573, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI17975520, Length=309, Percent_Identity=44.6601941747573, Blast_Score=273, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_METKA (Q8TZ08)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613420.1
- ProteinModelPortal:   Q8TZ08
- SMR:   Q8TZ08
- GeneID:   1477436
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0133
- NMPDR:   fig|190192.1.peg.133
- HOGENOM:   HBG397258
- OMA:   SGPTSHE
- ProtClustDB:   PRK04270
- BioCyc:   MKAN190192:MK0133-MONOMER
- HAMAP:   MF_01081
- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR004521
- SMART:   SM00359
- TIGRFAMs:   TIGR00425
- TIGRFAMs:   TIGR00431
- TIGRFAMs:   TIGR00451

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 37813; Mature: 37682

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50890 PUA

Important sites: ACT_SITE 80-80

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDKDRRLPFDRDREMITKAEVETDPRYGCPPEERPIEEYIMKGVINLDKPAGPTSHEV
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHH
VAWVKEIFGLSKAGHGGTLDPKVTGVLPIALEKATKIIQTLLPAGKEYVTIMHLHGDVDE
HHHHHHHHCCCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
EELERVVKEFEGTILQRPPLRSAVKRRVRPKKIYYIDILEIDGRDVLMRVGCQAGTYIRK
HHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHCCCCCHHHHH
LCHDIGEALGVGAHMAELRRTRTGPFSEENAVTLHDVKDAYEFWKEEGWEEPLRHVVRPM
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHH
EEGLEHLPRIEIRDTAVDAICHGANLAAPGIVRVEKGIQPGDLVAIFTLKGEAVALGVAK
HHHHHHCCCCEEHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEEEECCCEEEEHHHH
ATWKEMLHADRGIMVDTKRVLMEPGTYPKAWGLKTPGE
HHHHHHHHCCCCCEEECHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SGDKDRRLPFDRDREMITKAEVETDPRYGCPPEERPIEEYIMKGVINLDKPAGPTSHEV
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHH
VAWVKEIFGLSKAGHGGTLDPKVTGVLPIALEKATKIIQTLLPAGKEYVTIMHLHGDVDE
HHHHHHHHCCCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
EELERVVKEFEGTILQRPPLRSAVKRRVRPKKIYYIDILEIDGRDVLMRVGCQAGTYIRK
HHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHCCCCCHHHHH
LCHDIGEALGVGAHMAELRRTRTGPFSEENAVTLHDVKDAYEFWKEEGWEEPLRHVVRPM
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHH
EEGLEHLPRIEIRDTAVDAICHGANLAAPGIVRVEKGIQPGDLVAIFTLKGEAVALGVAK
HHHHHHCCCCEEHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEEEECCCEEEEHHHH
ATWKEMLHADRGIMVDTKRVLMEPGTYPKAWGLKTPGE
HHHHHHHHCCCCCEEECHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014