Click here to switch to the map view.

The map label for this gene is LysC [C]

Identifier: 20093549

GI number: 20093549

Start: 103269

End: 104672

Strand: Direct

Name: LysC [C]

Synonym: MK0109

Alternate gene names: 20093549

Gene position: 103269-104672 (Clockwise)

Preceding gene: 20093544

Following gene: 20093550

Centisome position: 6.09

GC content: 61.11

Gene sequence:

>1404_bases
TTGAGGGTCGTTATGAAGTTCGGCGGTACCTCGGTAGGTACCGGAGAGTCCATTAGGAAAGTTGCGAAAATCGTCACAGA
CGCGGCGGAGGAGCACGAGGTGATCGTGGTAGTTTCAGCGATGTCTGGGGTCACGGACGAGCTGGTACGGGCGGCCGAAA
GCGCCCCGGACTGGACGGAGGAGGATGTTAAAAACTTCGTAGGGAAGCTCCGAAGGAGGCACGGAAAAGCCGCTTCGGAA
GCGATTTCGTCCGACCTCATCCGGCGGGAGGTGATGGGTTACGTCGACTCCTTACTCGAGGAACTCGAGAAAGTGCTGTT
GGGCCTCAGTTACGTCGGTGAGGTGACCCCTCGTTCGATGGACCTCATACTCTCGTTCGGGGAGCGAATGTCCGCACCCA
TCGTAGCGGGCGCACTACGGGACCGAGGACTCGAGGCGGAGCATCTCGAGGGAGGAGAGGCTGGAGTGATCACGGACGAT
GGGTTCGGTGAGGCCGAGCCGATACTGCCCGCTTGCCGGCGGAAGGCGCAGAAGACGCTGATTCCCATGATCGAATCCGG
AAAGATCCCCGTGATCACGGGCTTCATCGGGCGGACTATCGACGGCGAGGTGACGACGCTGGGGCGCGGAGGATCCGACT
ACTCCGCCGCCATCATCGGGTGCATCTCGGAGGCGGACGAGGTGCAGATATGGACCGACGTCGACGGTGTGATGACGGCG
AACCCGAACCTCGTACCTGATGCCCGAACGGTCCCGAGGCTCTCGTACGAGGAGGCCATGGAACTGGCCAGCTTCGGCGC
GGAGGTACTCCATCCTAAGACCGTCATTCCGGCAAGGTCGGAAAACATCCCGATTCGGGTTAAGAACACCTTCAACCCCG
AGAGCGAGGGTACGCTGATCACTTCCGAATCGGAGCCTTCCGAGCAGGTGGTGAAGGCGGTCGCGTCGTCCTCGGACGTC
GGTATGATCGACATCCGAGGCACCACGATGATTGGGCGTCCCGGAGTGGCAGGACGAATATTCTCGAGACTCGGGGACGA
GGGAATCAACGTCATAATGATATCCCAGTCGGCCTCGGAGTCGAACATCTCGATAGTCGTGAGCCGCCCGGAGGTTCGCC
GCGCCGCTCGTATCATCGAGCGTGAGTTCGTCGGCGAGCGAGTGGTCGAGAGGGTCACGACGTACGAGGACGTAGCCGTG
GTGGCGGTGGTAGGGGAGGGCATGCGAGGGACTCCCGGAGTCGCTTCCAGGGTGTTCAGAGCGGTCGCGGACGCCGGGGT
AAACATCAAAACCATCTCGCAAGGGGCGTCTGAGGTGAACATATCGTTCGTAGTAGCGGAGGAGGACGAGGCGGCGGCGG
TTAACGCGGTTCACAGCGAATTCGAACTCGGTGAGGAGGCATGA

Upstream 100 bases:

>100_bases
GCCTCCTCGTCCGGGATCCACACCTTATCCTTACCCGCGTCCGACCTCTCGGCCTTGGCCACGTTCGTCACCCCGGAACC
CGCGAGTAGGGAGGAGGACC

Downstream 100 bases:

>100_bases
TCAGACGGCTGGTCGAGCGGTATCTCAGGCACGTCACCAAAAAGCGGCTGACACGCAGACCCACCACGGCGCTGATCTAC
ACGTGCCTCCTCGGGATGAA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MRVVMKFGGTSVGTGESIRKVAKIVTDAAEEHEVIVVVSAMSGVTDELVRAAESAPDWTEEDVKNFVGKLRRRHGKAASE
AISSDLIRREVMGYVDSLLEELEKVLLGLSYVGEVTPRSMDLILSFGERMSAPIVAGALRDRGLEAEHLEGGEAGVITDD
GFGEAEPILPACRRKAQKTLIPMIESGKIPVITGFIGRTIDGEVTTLGRGGSDYSAAIIGCISEADEVQIWTDVDGVMTA
NPNLVPDARTVPRLSYEEAMELASFGAEVLHPKTVIPARSENIPIRVKNTFNPESEGTLITSESEPSEQVVKAVASSSDV
GMIDIRGTTMIGRPGVAGRIFSRLGDEGINVIMISQSASESNISIVVSRPEVRRAARIIEREFVGERVVERVTTYEDVAV
VAVVGEGMRGTPGVASRVFRAVADAGVNIKTISQGASEVNISFVVAEEDEAAAVNAVHSEFELGEEA

Sequences:

>Translated_467_residues
MRVVMKFGGTSVGTGESIRKVAKIVTDAAEEHEVIVVVSAMSGVTDELVRAAESAPDWTEEDVKNFVGKLRRRHGKAASE
AISSDLIRREVMGYVDSLLEELEKVLLGLSYVGEVTPRSMDLILSFGERMSAPIVAGALRDRGLEAEHLEGGEAGVITDD
GFGEAEPILPACRRKAQKTLIPMIESGKIPVITGFIGRTIDGEVTTLGRGGSDYSAAIIGCISEADEVQIWTDVDGVMTA
NPNLVPDARTVPRLSYEEAMELASFGAEVLHPKTVIPARSENIPIRVKNTFNPESEGTLITSESEPSEQVVKAVASSSDV
GMIDIRGTTMIGRPGVAGRIFSRLGDEGINVIMISQSASESNISIVVSRPEVRRAARIIEREFVGERVVERVTTYEDVAV
VAVVGEGMRGTPGVASRVFRAVADAGVNIKTISQGASEVNISFVVAEEDEAAAVNAVHSEFELGEEA
>Mature_467_residues
MRVVMKFGGTSVGTGESIRKVAKIVTDAAEEHEVIVVVSAMSGVTDELVRAAESAPDWTEEDVKNFVGKLRRRHGKAASE
AISSDLIRREVMGYVDSLLEELEKVLLGLSYVGEVTPRSMDLILSFGERMSAPIVAGALRDRGLEAEHLEGGEAGVITDD
GFGEAEPILPACRRKAQKTLIPMIESGKIPVITGFIGRTIDGEVTTLGRGGSDYSAAIIGCISEADEVQIWTDVDGVMTA
NPNLVPDARTVPRLSYEEAMELASFGAEVLHPKTVIPARSENIPIRVKNTFNPESEGTLITSESEPSEQVVKAVASSSDV
GMIDIRGTTMIGRPGVAGRIFSRLGDEGINVIMISQSASESNISIVVSRPEVRRAARIIEREFVGERVVERVTTYEDVAV
VAVVGEGMRGTPGVASRVFRAVADAGVNIKTISQGASEVNISFVVAEEDEAAAVNAVHSEFELGEEA

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=465, Percent_Identity=35.2688172043011, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1790455, Length=465, Percent_Identity=30.752688172043, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1790376, Length=332, Percent_Identity=29.5180722891566, Blast_Score=115, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6320893, Length=498, Percent_Identity=29.3172690763052, Blast_Score=210, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 49822; Mature: 49822

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVMKFGGTSVGTGESIRKVAKIVTDAAEEHEVIVVVSAMSGVTDELVRAAESAPDWTE
CEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCH
EDVKNFVGKLRRRHGKAASEAISSDLIRREVMGYVDSLLEELEKVLLGLSYVGEVTPRSM
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DLILSFGERMSAPIVAGALRDRGLEAEHLEGGEAGVITDDGFGEAEPILPACRRKAQKTL
HHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
IPMIESGKIPVITGFIGRTIDGEVTTLGRGGSDYSAAIIGCISEADEVQIWTDVDGVMTA
HHHHCCCCCEEEEHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCEEEC
NPNLVPDARTVPRLSYEEAMELASFGAEVLHPKTVIPARSENIPIRVKNTFNPESEGTLI
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCEECCCCCCCCCEEEECCCCCCCCCEEE
TSESEPSEQVVKAVASSSDVGMIDIRGTTMIGRPGVAGRIFSRLGDEGINVIMISQSASE
ECCCCCHHHHHHHHHCCCCCEEEEECCEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCC
SNISIVVSRPEVRRAARIIEREFVGERVVERVTTYEDVAVVAVVGEGMRGTPGVASRVFR
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHH
AVADAGVNIKTISQGASEVNISFVVAEEDEAAAVNAVHSEFELGEEA
HHHHCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRVVMKFGGTSVGTGESIRKVAKIVTDAAEEHEVIVVVSAMSGVTDELVRAAESAPDWTE
CEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCH
EDVKNFVGKLRRRHGKAASEAISSDLIRREVMGYVDSLLEELEKVLLGLSYVGEVTPRSM
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DLILSFGERMSAPIVAGALRDRGLEAEHLEGGEAGVITDDGFGEAEPILPACRRKAQKTL
HHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
IPMIESGKIPVITGFIGRTIDGEVTTLGRGGSDYSAAIIGCISEADEVQIWTDVDGVMTA
HHHHCCCCCEEEEHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCEEEC
NPNLVPDARTVPRLSYEEAMELASFGAEVLHPKTVIPARSENIPIRVKNTFNPESEGTLI
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCEECCCCCCCCCEEEECCCCCCCCCEEE
TSESEPSEQVVKAVASSSDVGMIDIRGTTMIGRPGVAGRIFSRLGDEGINVIMISQSASE
ECCCCCHHHHHHHHHCCCCCEEEEECCEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCC
SNISIVVSRPEVRRAARIIEREFVGERVVERVTTYEDVAVVAVVGEGMRGTPGVASRVFR
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHH
AVADAGVNIKTISQGASEVNISFVVAEEDEAAAVNAVHSEFELGEEA
HHHHCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]