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The map label for this gene is rfcS

Identifier: 20093446

GI number: 20093446

Start: 5349

End: 7256

Strand: Reverse

Name: rfcS

Synonym: MK0006

Alternate gene names: 20093446

Gene position: 7256-5349 (Counterclockwise)

Preceding gene: 20093447

Following gene: 20093445

Centisome position: 0.43

GC content: 60.9

Gene sequence:

>1908_bases
ATGGCCGAGCACGAGCTACGCGTTCTCGAGATACCGTGGGTGGAAAAGTACCGGCCTAAGCGTCTGGACGACATCGTCGA
CCAGGAGCACGTGGTCGAGCGTCTGAAGGCCTACGTGAACCGCGGCGACATGCCCAACCTGCTCTTCGCGGGTCCACCTG
GAACCGGCAAAACGACGGCGGCTCTGTGCTTAGCGCGGGAACTGTTCGGTGAGCACTGGAGAGACAACTTCCTCGAGCTG
AACGCTTCCGTGTCCGCGGACACTCCGATCCTCGTGCGGAGGGGCGGCGAAGTCCTCCGGGTGACCTTCGAGGACCTCGA
CAGCTGGTACTTCGGGGATCGTGGCGGCGAGTACGTGGACGTGAGCGATTTGGAGGTTTTGACGGTCGATAGGAACTTCA
GGGTGACTTGGGCCCGCGTTTCCAAGCTCATCAGACACCGTGCCCGTAAGATCCTGCGCGTCCACCTCGAGGACGGAACG
ATCGAGCTCACGGGTAACCACGCGGTGATGGTACTGGACGAGGGCGGCCTCCGGGCGGTGAAGGCATCCGAGATAGAGGA
AGGCTCGTTTCTACTCAGCTTCGTCGCGGAACTCGACGAACAACCCACCGATGGCGGCACGGTGGTCACGTCGGTCGGAT
CCGGATCTCGGGTCTCGGACACCACCTACGAGCTGCCCGTGGAGGTTCGGGTCGAGCTCCTACGTGAGTTAGCCGATGAC
GGCGTGATCGAGGCCTCGGAGGACGTATCGGTGGATCTGGCGTGGCTGGCCCGGATCTCGGGTGTCGAGAGCCGTGTGAC
GGACGACGGTGTTGAGCTGGTTTGGGAGACCCGTACGGGCGACTTGTTACCGGCCGATCCGGTTCTGAAACTCGTCGAAA
GGCTCGAATCTGACCTCGTGGACGATCTCGAGTCGTGGGTGTTCGACGGTCGGGTATCGAAAGAGGCCGTCCGAAAGGTC
CTCTCCTCGGTCGATGCGAAAAATCTCCGTGGGGACGCTAGGAGAGCGTACCGGATGCTCAGGACGCTCGTACGATCGGA
CGTGCACGCCGTTAAGGTCGAGGATCTGGACGTCATGGACTACGACGGCTACGTCTACGACGTGAGCGTACCCGGCAACG
AGATGTTCTTCGCGGGTGAGGTTCCGGTCCTGCTGCACAACTCCGATGAGAGGGGTATCGACGTAATTCGGACCAAGGTG
AAGAACTTCGCCAGGACCCGGCCGATGGGAGGGGCCCGGTTCAAGATAATCTTCCTCGATGAGGCGGATAACCTGACCAG
GGACTCGCAACAGGCCCTCCGTAGAATTATGGAGATGTACTCCGACGCGTGTCGGTTCATACTCGCAGCGAACTACTCTT
CCGCGATCATCGACCCCATCCAGAGCCGGTGTGTAGTGTTTAAGTTCACCAAGCTACCCGAGAGCGCCATCAAGGAGCGG
TTAAGGAAGATCGCTGAGAGTGAAGGAGTCGAGATAACGGAGGACGCCCTGGACGCGATCGTTTACGTCTCCGAGGGCGA
TATGCGGCGTGCCATTAACGTACTCCAAGCTGCCGCGGCGCTCGGACGGGAGATCGACGAGGATACGGTGTTCCAGATCG
CGGCTACGGCTCGCCCCGAGGAGGTGCGGGAGATGATCCACCACGCCTGGAACGGGGACTTCGAGCGGGCCAGAGAACTA
CTACACGAGCTCCTCACCAGGTACGGAATGTCGGGAGAGGACGTCGTCCGTCAGGTACACCGGGAAATCTTCGACATGGA
CGAGATACCGGAGGAGGCAATTCCGGAGCTTGTGAACGCCGTCGGTGACTTCGAGTACCGCCTGATCCGCGGCTCGGACG
AGCGGATCCAGTTAGAGGCCCTGTTAGCCCGAATTCACGCACTCGGTAACGAGTACTCCGGCGGGTAG

Upstream 100 bases:

>100_bases
ATGCGGAGGGAGCTGCCAGTCATGAAGTACCTGACCGGCTGATCTCCTCCGACCGAATCGGTTTTTATCACGGGCGATGT
GATCCCCCGGGGACGTCGAG

Downstream 100 bases:

>100_bases
CACCGTTGGTCCCTTGGGTTGAGAAGTACCGACCTAGGTCGCTCAAAGAGCTGGTGAACCAGGACGAGGCCAAGAAGGAG
CTAGCCGCATGGGCGAACGA

Product: replication factor C (DNA replication ATPase) intein containing

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: RFC small subunit; Clamp loader small subunit

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MAEHELRVLEIPWVEKYRPKRLDDIVDQEHVVERLKAYVNRGDMPNLLFAGPPGTGKTTAALCLARELFGEHWRDNFLEL
NASVSADTPILVRRGGEVLRVTFEDLDSWYFGDRGGEYVDVSDLEVLTVDRNFRVTWARVSKLIRHRARKILRVHLEDGT
IELTGNHAVMVLDEGGLRAVKASEIEEGSFLLSFVAELDEQPTDGGTVVTSVGSGSRVSDTTYELPVEVRVELLRELADD
GVIEASEDVSVDLAWLARISGVESRVTDDGVELVWETRTGDLLPADPVLKLVERLESDLVDDLESWVFDGRVSKEAVRKV
LSSVDAKNLRGDARRAYRMLRTLVRSDVHAVKVEDLDVMDYDGYVYDVSVPGNEMFFAGEVPVLLHNSDERGIDVIRTKV
KNFARTRPMGGARFKIIFLDEADNLTRDSQQALRRIMEMYSDACRFILAANYSSAIIDPIQSRCVVFKFTKLPESAIKER
LRKIAESEGVEITEDALDAIVYVSEGDMRRAINVLQAAAALGREIDEDTVFQIAATARPEEVREMIHHAWNGDFERAREL
LHELLTRYGMSGEDVVRQVHREIFDMDEIPEEAIPELVNAVGDFEYRLIRGSDERIQLEALLARIHALGNEYSGG

Sequences:

>Translated_635_residues
MAEHELRVLEIPWVEKYRPKRLDDIVDQEHVVERLKAYVNRGDMPNLLFAGPPGTGKTTAALCLARELFGEHWRDNFLEL
NASVSADTPILVRRGGEVLRVTFEDLDSWYFGDRGGEYVDVSDLEVLTVDRNFRVTWARVSKLIRHRARKILRVHLEDGT
IELTGNHAVMVLDEGGLRAVKASEIEEGSFLLSFVAELDEQPTDGGTVVTSVGSGSRVSDTTYELPVEVRVELLRELADD
GVIEASEDVSVDLAWLARISGVESRVTDDGVELVWETRTGDLLPADPVLKLVERLESDLVDDLESWVFDGRVSKEAVRKV
LSSVDAKNLRGDARRAYRMLRTLVRSDVHAVKVEDLDVMDYDGYVYDVSVPGNEMFFAGEVPVLLHNSDERGIDVIRTKV
KNFARTRPMGGARFKIIFLDEADNLTRDSQQALRRIMEMYSDACRFILAANYSSAIIDPIQSRCVVFKFTKLPESAIKER
LRKIAESEGVEITEDALDAIVYVSEGDMRRAINVLQAAAALGREIDEDTVFQIAATARPEEVREMIHHAWNGDFERAREL
LHELLTRYGMSGEDVVRQVHREIFDMDEIPEEAIPELVNAVGDFEYRLIRGSDERIQLEALLARIHALGNEYSGG
>Mature_634_residues
AEHELRVLEIPWVEKYRPKRLDDIVDQEHVVERLKAYVNRGDMPNLLFAGPPGTGKTTAALCLARELFGEHWRDNFLELN
ASVSADTPILVRRGGEVLRVTFEDLDSWYFGDRGGEYVDVSDLEVLTVDRNFRVTWARVSKLIRHRARKILRVHLEDGTI
ELTGNHAVMVLDEGGLRAVKASEIEEGSFLLSFVAELDEQPTDGGTVVTSVGSGSRVSDTTYELPVEVRVELLRELADDG
VIEASEDVSVDLAWLARISGVESRVTDDGVELVWETRTGDLLPADPVLKLVERLESDLVDDLESWVFDGRVSKEAVRKVL
SSVDAKNLRGDARRAYRMLRTLVRSDVHAVKVEDLDVMDYDGYVYDVSVPGNEMFFAGEVPVLLHNSDERGIDVIRTKVK
NFARTRPMGGARFKIIFLDEADNLTRDSQQALRRIMEMYSDACRFILAANYSSAIIDPIQSRCVVFKFTKLPESAIKERL
RKIAESEGVEITEDALDAIVYVSEGDMRRAINVLQAAAALGREIDEDTVFQIAATARPEEVREMIHHAWNGDFERARELL
HELLTRYGMSGEDVVRQVHREIFDMDEIPEEAIPELVNAVGDFEYRLIRGSDERIQLEALLARIHALGNEYSGG

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA

COG id: COG1372

COG function: function code L; Intein/homing endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcS subfamily

Homologues:

Organism=Homo sapiens, GI31563534, Length=246, Percent_Identity=41.0569105691057, Blast_Score=178, Evalue=2e-44,
Organism=Homo sapiens, GI194306571, Length=245, Percent_Identity=37.5510204081633, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI194306567, Length=245, Percent_Identity=37.5510204081633, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI6677723, Length=245, Percent_Identity=37.5510204081633, Blast_Score=176, Evalue=8e-44,
Organism=Homo sapiens, GI194306569, Length=239, Percent_Identity=38.0753138075314, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI4506491, Length=251, Percent_Identity=36.2549800796813, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI31881687, Length=251, Percent_Identity=36.2549800796813, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI28882049, Length=205, Percent_Identity=38.0487804878049, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI4506489, Length=255, Percent_Identity=27.4509803921569, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI108773789, Length=127, Percent_Identity=32.2834645669291, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71986063, Length=243, Percent_Identity=37.4485596707819, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17541988, Length=243, Percent_Identity=36.6255144032922, Blast_Score=172, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17554730, Length=236, Percent_Identity=32.2033898305085, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17541990, Length=239, Percent_Identity=31.3807531380753, Blast_Score=108, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324478, Length=250, Percent_Identity=38, Blast_Score=180, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6324039, Length=248, Percent_Identity=33.0645161290323, Blast_Score=156, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6322528, Length=260, Percent_Identity=36.5384615384615, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6319562, Length=223, Percent_Identity=25.1121076233184, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17647857, Length=244, Percent_Identity=40.9836065573771, Blast_Score=182, Evalue=9e-46,
Organism=Drosophila melanogaster, GI19921076, Length=240, Percent_Identity=35.8333333333333, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI18859927, Length=247, Percent_Identity=37.6518218623482, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19921136, Length=242, Percent_Identity=28.5123966942149, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFCS_METKA (Q8TZC4)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613293.1
- ProteinModelPortal:   Q8TZC4
- GeneID:   1477308
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0006
- NMPDR:   fig|190192.1.peg.6
- HOGENOM:   HBG120083
- OMA:   EMFFAGE
- ProtClustDB:   CLSK519869
- BioCyc:   MKAN190192:MK0006-MONOMER
- HAMAP:   MF_01509
- InterPro:   IPR003593
- InterPro:   IPR008824
- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR006141
- InterPro:   IPR013748
- PRINTS:   PR00379
- SMART:   SM00382
- SMART:   SM00305
- SMART:   SM00306
- TIGRFAMs:   TIGR01443
- TIGRFAMs:   TIGR01445

Pfam domain/function: PF06144 DNA_pol3_delta; PF08542 Rep_fac_C; PF05496 RuvB_N; SSF48019 Pol_clamp_load_C

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 71587; Mature: 71456

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS50818 INTEIN_C_TER; PS50817 INTEIN_N_TER; PS00039 DEAD_ATP_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHELRVLEIPWVEKYRPKRLDDIVDQEHVVERLKAYVNRGDMPNLLFAGPPGTGKTTA
CCCCCEEEEECCCHHHCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
ALCLARELFGEHWRDNFLELNASVSADTPILVRRGGEVLRVTFEDLDSWYFGDRGGEYVD
HHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEECCCCEEEEEHHHHCCCCCCCCCCCEEE
VSDLEVLTVDRNFRVTWARVSKLIRHRARKILRVHLEDGTIELTGNHAVMVLDEGGLRAV
CCCCEEEEECCCCEEEHHHHHHHHHHHHHHHEEEEECCCEEEEECCEEEEEEECCCCEEE
KASEIEEGSFLLSFVAELDEQPTDGGTVVTSVGSGSRVSDTTYELPVEVRVELLRELADD
ECCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHCC
GVIEASEDVSVDLAWLARISGVESRVTDDGVELVWETRTGDLLPADPVLKLVERLESDLV
CCEECCCCCCCHHHHHHHHHCCHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
DDLESWVFDGRVSKEAVRKVLSSVDAKNLRGDARRAYRMLRTLVRSDVHAVKVEDLDVMD
HHHHHHHHCCCCCHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEE
YDGYVYDVSVPGNEMFFAGEVPVLLHNSDERGIDVIRTKVKNFARTRPMGGARFKIIFLD
CCCEEEEEECCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEE
EADNLTRDSQQALRRIMEMYSDACRFILAANYSSAIIDPIQSRCVVFKFTKLPESAIKER
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEECCCCHHHHHHH
LRKIAESEGVEITEDALDAIVYVSEGDMRRAINVLQAAAALGREIDEDTVFQIAATARPE
HHHHHHHCCCEEEHHHHCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCHH
EVREMIHHAWNGDFERARELLHELLTRYGMSGEDVVRQVHREIFDMDEIPEEAIPELVNA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHCCCHHHHHHHHHH
VGDFEYRLIRGSDERIQLEALLARIHALGNEYSGG
HCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AEHELRVLEIPWVEKYRPKRLDDIVDQEHVVERLKAYVNRGDMPNLLFAGPPGTGKTTA
CCCCEEEEECCCHHHCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
ALCLARELFGEHWRDNFLELNASVSADTPILVRRGGEVLRVTFEDLDSWYFGDRGGEYVD
HHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEECCCCEEEEEHHHHCCCCCCCCCCCEEE
VSDLEVLTVDRNFRVTWARVSKLIRHRARKILRVHLEDGTIELTGNHAVMVLDEGGLRAV
CCCCEEEEECCCCEEEHHHHHHHHHHHHHHHEEEEECCCEEEEECCEEEEEEECCCCEEE
KASEIEEGSFLLSFVAELDEQPTDGGTVVTSVGSGSRVSDTTYELPVEVRVELLRELADD
ECCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHCC
GVIEASEDVSVDLAWLARISGVESRVTDDGVELVWETRTGDLLPADPVLKLVERLESDLV
CCEECCCCCCCHHHHHHHHHCCHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
DDLESWVFDGRVSKEAVRKVLSSVDAKNLRGDARRAYRMLRTLVRSDVHAVKVEDLDVMD
HHHHHHHHCCCCCHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEE
YDGYVYDVSVPGNEMFFAGEVPVLLHNSDERGIDVIRTKVKNFARTRPMGGARFKIIFLD
CCCEEEEEECCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEE
EADNLTRDSQQALRRIMEMYSDACRFILAANYSSAIIDPIQSRCVVFKFTKLPESAIKER
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEECCCCHHHHHHH
LRKIAESEGVEITEDALDAIVYVSEGDMRRAINVLQAAAALGREIDEDTVFQIAATARPE
HHHHHHHCCCEEEHHHHCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCHH
EVREMIHHAWNGDFERARELLHELLTRYGMSGEDVVRQVHREIFDMDEIPEEAIPELVNA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHCCCHHHHHHHHHH
VGDFEYRLIRGSDERIQLEALLARIHALGNEYSGG
HCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014