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The map label for this gene is pbpX [H]

Identifier: 19746581

GI number: 19746581

Start: 1393952

End: 1396207

Strand: Reverse

Name: pbpX [H]

Synonym: spyM18_1674

Alternate gene names: 19746581

Gene position: 1396207-1393952 (Counterclockwise)

Preceding gene: 19746582

Following gene: 19746580

Centisome position: 73.68

GC content: 37.46

Gene sequence:

>2256_bases
ATGAAAAAATGGCAAAAATATGTTTTAGATTATGTTGTGCGCGATAGGAGAACTCCAGTCGAAAATCGCGTTCGAGTTGG
ACAAAATATGATGCTCTTAACTATCTTTATTTTCTTTATTTTCATTATTAATTTCATGATTATTATTGGAACAGATCAAA
AGTTTGGAGTTAGTTTGTCAGAAGGGGCGAAGAAGGTTTATCAAGAAACCGTTACGATCCAAGCTAAGCGTGGGACCATT
TATGATCGAAATGGTACAGCTATTGCAGTGGATTCTACGACTTATAGCATATACGCAATTTTGGATAAATCATTTGTCTC
GGCTTCAGATGAAAAGTTATATGTACAACCTTCTCAGTATGAAACAGTAGCTGATATTTTAAAAAAGCATCTGGGAATGA
AAAAAACAGATGTAATTAAACAGCTTAAGCGTAAAGGACTTTTCCAAGTCTCGTTTGGACCGTCAGGGTCTGGCATTTCA
TATAGTACTATGTCTACTATTCAAAAGGCTATGGAAGATGCCAAAATCAAGGGAATTGCTTTCACAACTAGTCCTGGTCG
TATGTATCCAAATGGGACATTTGCTTCAGAATTTATAGGCTTAGCATCTCTAACAGAAGATAAAAAGACAGGTGTTAAGA
GTTTAGTTGGAAAAACAGGTCTAGAAGCTTCTTTTGATAAAATTTTATCAGGTCAAGATGGTGTTATTACTTATCAAAAA
GATCGAAATGGGACCACACTCTTGGGTACAGGTAAGACTGTCAAGAAAGCTATTGATGGCAAAGATATTTACACAACGCT
ATCTGAGCCTATCCAGACCTTCTTAGAAACCCAGATGGATGTTTTTCAAGCCAAATCAAATGGTCAGTTGGCCAGTGCAA
CACTTGTTAATGCTAAAACTGGTGAAATTTTGGCAACAACACAACGCCCCACTTATAATGCTGATACTCTGAAAGGACTT
GAAAATACGAACTACAAATGGTACAGTGCACTTCATCAAGGAAATTTTGAGCCAGGCTCAACCATGAAAGTGATGACTCT
GGCAGCGGCTATTGATGATAAAGTTTTCAACCCAAACGAAACCTTTAGCAATGCTAATGGTTTAACAATTGCAGATGCTA
CTATTCAAGACTGGTCTATTAACGAAGGCATTTCCACAGGACAGTACATGAATTATGCACAAGGGTTTGCCTTCTCAAGT
AACGTTGGGATGACTAAACTTGAACAAAAAATGGGTAATGCAAAATGGATGAATTATTTGACGAAGTTCCGCTTTGGTTT
TCCTACTCGTTTTGGCTTAAAAGATGAAGACGCAGGTATATTTCCTTCTGATAATATCGTGACTCAAGCTATGAGCGCTT
TTGGTCAAGGGATTTCTGTAACCCAGATTCAAATGCTTAGAGCCTTTACTGCTATTTCTAATAATGGTGAGATGTTAGAG
CCACAATTTATCAGTCAAATTTATGATCCTAACACAGCAAGTTTTAGAACGGCAAATAAAGAAATTGTTGGAAAACCTGT
ATCAAAAAAAGCCGCTAGTGAAACAAGACAATACATGATTGGTGTAGGAACAGACCCTGAGTTTGGAACACTCTATTCAA
AAACATTTGGACCAATTATTAAAGTGGGTGATTTACCTGTTGCTGTTAAATCAGGAACAGCACAAATTGGTTCAGAAGAT
GGAAGTGGTTATCAAGATGGTGGATTGACTAACTATGTCTATTCGGTTGTGGCAATGGTGCCAGCTGATAAACCAGACTT
TTTGATGTATGTTACTATGACTAAACCACAACATTTTGGTCCCCTTTTTTGGCAAGATGTGGTTAACCCAGTATTGGAAG
AAGCATACTTAATGCAAGATACACTAACTAAGCCAGTAGTATCAGATGCTAATCGTCAAACAACTTATAAATTACCAAAC
TTTGTAGGAAAGAATCCTGGTGAGACATCAAGCGAGTTGCGTCGAAATCTTGTCCAGCCAGTTGTCCTTGGTACTGGCAG
CAAGATCAAAAAAGTATCGCATCAGCCCGGTCAAACGTTAACAGAAAACCAACAAGTTCTCATATTATCAGACCGTTTTG
TGGAGGTACCAGACATGTATGGCTGGACAAAATCCAATGTTAAAACCTTTGCTAAATGGACTGGGATAGACATCAGCTTT
AAAGGAACAGATTCTGGTCGTGTTATGAAACAAAGTGTTGATGTTGGTAAGTCCTTGAAAAAAATAAAAAAAATGACCAT
TACTTTAGGAGATTAA

Upstream 100 bases:

>100_bases
AGAAGTAAATGAGTTATCTCGCCGTGATCGTATTATAGATATTGCTGGTAAAGCAGGCTTAAGCAACCGTAATAATAATA
TCAAGAAAGTCGAGTAATCT

Downstream 100 bases:

>100_bases
CATGTTTTTAACCCTTATTGCAGCAATCATATCCTTTATGGTATCTGCTTTCACAATGCCTTATTTTATCAAATTCTATC
AATTGAAAAAAATTGGTGGG

Product: penicillin binding protein

Products: NA

Alternate protein names: PBP-2x; PBP2x [H]

Number of amino acids: Translated: 751; Mature: 751

Protein sequence:

>751_residues
MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIINFMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTI
YDRNGTAIAVDSTTYSIYAILDKSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGKTGLEASFDKILSGQDGVITYQK
DRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLETQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGL
ENTNYKWYSALHQGNFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNYAQGFAFSS
NVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQAMSAFGQGISVTQIQMLRAFTAISNNGEMLE
PQFISQIYDPNTASFRTANKEIVGKPVSKKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSED
GSGYQDGGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTKPVVSDANRQTTYKLPN
FVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTENQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISF
KGTDSGRVMKQSVDVGKSLKKIKKMTITLGD

Sequences:

>Translated_751_residues
MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIINFMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTI
YDRNGTAIAVDSTTYSIYAILDKSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGKTGLEASFDKILSGQDGVITYQK
DRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLETQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGL
ENTNYKWYSALHQGNFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNYAQGFAFSS
NVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQAMSAFGQGISVTQIQMLRAFTAISNNGEMLE
PQFISQIYDPNTASFRTANKEIVGKPVSKKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSED
GSGYQDGGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTKPVVSDANRQTTYKLPN
FVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTENQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISF
KGTDSGRVMKQSVDVGKSLKKIKKMTITLGD
>Mature_751_residues
MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIINFMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTI
YDRNGTAIAVDSTTYSIYAILDKSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGKTGLEASFDKILSGQDGVITYQK
DRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLETQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGL
ENTNYKWYSALHQGNFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNYAQGFAFSS
NVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQAMSAFGQGISVTQIQMLRAFTAISNNGEMLE
PQFISQIYDPNTASFRTANKEIVGKPVSKKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSED
GSGYQDGGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTKPVVSDANRQTTYKLPN
FVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTENQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISF
KGTDSGRVMKQSVDVGKSLKKIKKMTITLGD

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactams inactivate the PBPs by acylating an essential serine residue in the active site of these proteins [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=538, Percent_Identity=25.4646840148699, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1786854, Length=565, Percent_Identity=22.3008849557522, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 82801; Mature: 82801

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIINFMIIIGTDQKFGVSLS
CCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCHH
EGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILDKSFVSASDEKLYVQPSQY
HHHHHHHHHHEEEEEECCEEEECCCCEEEEECCCEEEEEEEEHHHHCCCCCEEEECCCHH
ETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGISYSTMSTIQKAMEDAKIKGIA
HHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEE
FTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGKTGLEASFDKILSGQDGVITYQK
EECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEE
DRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLETQMDVFQAKSNGQLASATLVNAKT
CCCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCC
GEILATTQRPTYNADTLKGLENTNYKWYSALHQGNFEPGSTMKVMTLAAAIDDKVFNPNE
CCEEEECCCCCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC
TFSNANGLTIADATIQDWSINEGISTGQYMNYAQGFAFSSNVGMTKLEQKMGNAKWMNYL
CCCCCCCCEEEECEEECCCCCCCCCCCHHHHHHHCCEEECCCCHHHHHHHHCCHHHHHHH
TKFRFGFPTRFGLKDEDAGIFPSDNIVTQAMSAFGQGISVTQIQMLRAFTAISNNGEMLE
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEC
PQFISQIYDPNTASFRTANKEIVGKPVSKKAASETRQYMIGVGTDPEFGTLYSKTFGPII
HHHHHHHCCCCCCCEECCCHHHCCCCHHHHHHHHHHEEEEECCCCCCCCHHHHHHCCCEE
KVGDLPVAVKSGTAQIGSEDGSGYQDGGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFG
EECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
PLFWQDVVNPVLEEAYLMQDTLTKPVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQP
CHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEECCHHCCCCCCCCHHHHHHHHHCC
VVLGTGSKIKKVSHQPGQTLTENQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISF
EEECCCHHHHHHCCCCCCCCCCCCEEEEEECCCEECCCCCCCCHHCHHHHHEEECEEEEE
KGTDSGRVMKQSVDVGKSLKKIKKMTITLGD
ECCCCCHHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIINFMIIIGTDQKFGVSLS
CCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCHH
EGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILDKSFVSASDEKLYVQPSQY
HHHHHHHHHHEEEEEECCEEEECCCCEEEEECCCEEEEEEEEHHHHCCCCCEEEECCCHH
ETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGISYSTMSTIQKAMEDAKIKGIA
HHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEE
FTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGKTGLEASFDKILSGQDGVITYQK
EECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEE
DRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLETQMDVFQAKSNGQLASATLVNAKT
CCCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCC
GEILATTQRPTYNADTLKGLENTNYKWYSALHQGNFEPGSTMKVMTLAAAIDDKVFNPNE
CCEEEECCCCCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC
TFSNANGLTIADATIQDWSINEGISTGQYMNYAQGFAFSSNVGMTKLEQKMGNAKWMNYL
CCCCCCCCEEEECEEECCCCCCCCCCCHHHHHHHCCEEECCCCHHHHHHHHCCHHHHHHH
TKFRFGFPTRFGLKDEDAGIFPSDNIVTQAMSAFGQGISVTQIQMLRAFTAISNNGEMLE
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEC
PQFISQIYDPNTASFRTANKEIVGKPVSKKAASETRQYMIGVGTDPEFGTLYSKTFGPII
HHHHHHHCCCCCCCEECCCHHHCCCCHHHHHHHHHHEEEEECCCCCCCCHHHHHHCCCEE
KVGDLPVAVKSGTAQIGSEDGSGYQDGGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFG
EECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
PLFWQDVVNPVLEEAYLMQDTLTKPVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQP
CHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEECCHHCCCCCCCCHHHHHHHHHCC
VVLGTGSKIKKVSHQPGQTLTENQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISF
EEECCCHHHHHHCCCCCCCCCCCCEEEEEECCCEECCCCCCCCHHCHHHHHEEECEEEEE
KGTDSGRVMKQSVDVGKSLKKIKKMTITLGD
ECCCCCHHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11463916; 8605631 [H]