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The map label for this gene is fmt [H]

Identifier: 19746553

GI number: 19746553

Start: 1362721

End: 1363656

Strand: Reverse

Name: fmt [H]

Synonym: spyM18_1638

Alternate gene names: 19746553

Gene position: 1363656-1362721 (Counterclockwise)

Preceding gene: 19746554

Following gene: 19746552

Centisome position: 71.96

GC content: 38.03

Gene sequence:

>936_bases
ATGATAAAATTATTATTTATGGGAACTCCCCAATTTTCAGCAACTGTATTGAAGGGCTTGCTAGATAATCCTGCCTATGA
GATTTTAGGAGTGGTTACTCAGCCTGATCGAGCTGTTGGACGCAAAAAAGATATTAAGGTAACACCTGTAAAGCAATTAG
CTCTTGAGCATGGAATATCAATCTATCAGCCGGAAAAATTATCAGGCTCTCAAGAACTCATTGAGATTATGGGACTTGGT
GCAGATGGTATTATTACAGCAGCATTTGGCCAATTTTTACCAACACTATTACTTGATTCAGTTTCATTTGCTATTAATGT
ACATGCATCATTGTTACCTAAATATCGAGGTGGAGCACCTATCCATTATGCTATTATGAATGGAGATAAAGAGGCTGGCG
TTACTATTATGGAAATGATTAAAGAAATGGATGCAGGTGACATGGTCGCTAAAGCAAGCACTCCAATTCTTGAAACCGAT
AATGTAGGTACTCTTTTTGAGAAATTAGCCATCATTGGTCGAGACTTACTTTTGGATAGTTTGCCAGCTTATCTTTCTGG
AGAATTAAAACCTATTCCTCAAGATCACAGCCAAGCTACTTTTTCACCTAATATCTCTCCAGAACAAGAAAAATTAGATT
GGACAATGTCTAATCAAGAAGTGTTCAGTCATATTCGAGGTATGAACCCTTGGCCGGTAGCGCATACGTTTTTAGAGGGG
CAACGTTTAAAAATTTACGAAGCTCAACTAGCTGAAGGAGAAGGATTACCTGGACAAGTTATTGTCAAAACCAAAAAATC
TTTGGTAATAGCTACTGGACAAGGAGCTTTGTCCTTGATCGTTGTGCAACCGGCTGGTAAACCTAAAATGTCAATAATAG
ACTTTTTAAACGGCATTGGTAGAAAACTTGAAGTAGGGGACATCATTGGCAGATAA

Upstream 100 bases:

>100_bases
AAACTAATTATTGATTGTGAACCGCAACAATTTTTATAAGGTAAATCACTAAATCTAGGATGATCAGTTATGATATAATA
AAAATACAAAGAGGAAAGTC

Downstream 100 bases:

>100_bases
TTGGAAAAAAAGTACGCGAGGCAAAGCCCTCCTTGTCATTGAAGCTATTTTTGATCAGGGGGCTTACACAAACATAGCGC
TTAATCAGCAGCTAAGCAAT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKDIKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLG
ADGIITAAFGQFLPTLLLDSVSFAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSNQEVFSHIRGMNPWPVAHTFLEG
QRLKIYEAQLAEGEGLPGQVIVKTKKSLVIATGQGALSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR

Sequences:

>Translated_311_residues
MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKDIKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLG
ADGIITAAFGQFLPTLLLDSVSFAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSNQEVFSHIRGMNPWPVAHTFLEG
QRLKIYEAQLAEGEGLPGQVIVKTKKSLVIATGQGALSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR
>Mature_311_residues
MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKDIKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLG
ADGIITAAFGQFLPTLLLDSVSFAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSNQEVFSHIRGMNPWPVAHTFLEG
QRLKIYEAQLAEGEGLPGQVIVKTKKSLVIATGQGALSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI21614513, Length=250, Percent_Identity=26, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI164663775, Length=323, Percent_Identity=28.4829721362229, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI238814322, Length=265, Percent_Identity=25.2830188679245, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789683, Length=299, Percent_Identity=39.4648829431438, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788589, Length=262, Percent_Identity=32.0610687022901, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI133930964, Length=275, Percent_Identity=25.4545454545455, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319458, Length=353, Percent_Identity=25.2124645892351, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45550868, Length=309, Percent_Identity=30.42071197411, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI28571984, Length=224, Percent_Identity=32.5892857142857, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585660, Length=264, Percent_Identity=25.3787878787879, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 33649; Mature: 33649

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKDIKVTPVKQLALEHGIS
CEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCCCEECHHHHHHHHCCCC
IYQPEKLSGSQELIEIMGLGADGIITAAFGQFLPTLLLDSVSFAINVHASLLPKYRGGAP
EECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCCC
IHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETDNVGTLFEKLAIIGRDLLLDS
EEEEEECCCCCCCHHHHHHHHHCCCCCEEHHCCCCCEECCCHHHHHHHHHHHHHHHHHHH
LPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSNQEVFSHIRGMNPWPVAHTFLEG
CCHHHCCCCCCCCCCCCCCEECCCCCCCHHHCCEECCCHHHHHHHCCCCCCCHHHHHHCC
QRLKIYEAQLAEGEGLPGQVIVKTKKSLVIATGQGALSLIVVQPAGKPKMSIIDFLNGIG
CEEEEEEEHHHCCCCCCCEEEEEECCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHCC
RKLEVGDIIGR
CEEEHHHHCCC
>Mature Secondary Structure
MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKDIKVTPVKQLALEHGIS
CEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCCCEECHHHHHHHHCCCC
IYQPEKLSGSQELIEIMGLGADGIITAAFGQFLPTLLLDSVSFAINVHASLLPKYRGGAP
EECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCCC
IHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETDNVGTLFEKLAIIGRDLLLDS
EEEEEECCCCCCCHHHHHHHHHCCCCCEEHHCCCCCEECCCHHHHHHHHHHHHHHHHHHH
LPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSNQEVFSHIRGMNPWPVAHTFLEG
CCHHHCCCCCCCCCCCCCCEECCCCCCCHHHCCEECCCHHHHHHHCCCCCCCHHHHHHCC
QRLKIYEAQLAEGEGLPGQVIVKTKKSLVIATGQGALSLIVVQPAGKPKMSIIDFLNGIG
CEEEEEEEHHHCCCCCCCEEEEEECCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHCC
RKLEVGDIIGR
CEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]