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The map label for this gene is priA [H]

Identifier: 19746554

GI number: 19746554

Start: 1363718

End: 1366102

Strand: Reverse

Name: priA [H]

Synonym: spyM18_1639

Alternate gene names: 19746554

Gene position: 1366102-1363718 (Counterclockwise)

Preceding gene: 19746555

Following gene: 19746553

Centisome position: 72.09

GC content: 38.99

Gene sequence:

>2385_bases
ATGGAAAAAGTAGCTCATGTGATTGTAGATATTCCATTGATGCAAACGGACAAGCCATTTTCTTATGGTATTCCAAAAGA
GTTAGTTTCTCTCGTGCAGCTTGGTTCTCGTGTGCATGTTCCTTTTGGAAAAGGGAATCGTTTGTTGCAAGGCTTTATTA
TTGGTTTTGGCCAAGAAGATTCAAGCTCTCTTAAACTGATTCAGACCGTTTTAGATCCTGAGCCAGTCCTAAACCAAGAG
CAATTGACCCTTGCTGATCAGCTTCGAAAAACAGTTTTTTCTTATAAAATCACGCTCTTAAAAGCAATGATTCCTAATCT
TCTTAACTCTAACTATGATAAAGTGCTAAGGCCAGAGTCAGGTCTAAAAAAGCCTGATAGAGACTTTCTTTTTGAAGGAC
AACCATCTGTCTTATATTCTACCTTAGACCGCGAGAAGGGAAAAATAGCTCTTAAGGGCATACAAGCTGGGCATATTACT
GTTAGTTATTTAGCCAAAGACAAGAAAAACCTAAAGACAGAAAAATATTATCACGTCGACTTAGACGCCTTAGCGGTACA
TCCCATCTCTAGTCGTGCCAAAAAGCGTCAATTATTAAAAGACTATCTGCTAACCCATACGAAGGAAGCTAAACTTGCAA
CGCTTTATCAAGCCTTTTCTAGAGATGTGGTGACTTACTTTGTGACAAATCACTTAATTCGTATTGACGAACGTCCAATT
GATCGCAGTGAGTCCTATTTTGACCAGATCAAACCATCTTCTTTTTTAACCTTAAATGAACAGCAGGCGTCAGCTGTTAC
TGAAATTGTTGAACAAATTGGGAAACCGAGTAAACCTTTTTTGATTGAAGGGATTACAGGTTCGGGTAAGACAGAAGTTT
ATCTTCATATCATCGAAGCGGTTTTAAAACAGGATAAGACAGCCATTGTTTTAGTGCCAGAAATTTCGTTGACTCCTCAG
ATGACCTCTCGTTTTATTTCCCGTTTCGGAAAGCAAGTGGCTATTATGCATTCAGGCTTATCAGATGGTGAAAAGTTTGA
TGAGTGGCGCAAGATAAAAACAGGACAGGCTAAGGTTGTGGTTGGTGCCCGCTCTGCTATTTTCTCCCCACTAGAGCGGA
TTGGTGCAATTATTATCGATGAGGAGCATGAATCAACCTATAAACAAGAAAGTAATCCACGATACCATGCCAGAGAGGTT
GCCCTGCTCAGAGCTAAACACCATCAAGCAGTTGTGGTTATGGGCTCTGCTACGCCAAGTATTGAAAGCCGAGCAAGGGC
TAGTAAGGGTGTCTATCATTTTATTCAATTAACGCAAAGAGCAAACCCTCTCGCTAAGATTCCAGAAGTAACGATTGTTG
ATTTTCGTGATTATATTGGACAGCAAGCAGTTAGTAATTTTACCCCATATTTGATTGATAAGATTAAAGAACGTTTGGTT
AAAAAAGAACAAGTTGTCTTAATGTTAAATAGACGTGGTTATTCTAGTTTTGTGATGTGTCGGGACTGTGGTTATGTGGA
CAAATGTCCCAACTGTGATATCTCTTTAACCCTTCACATGGATACTAAAACTATGAATTGCCATTACTGTGGGTTTCAAA
AACCTATCCCAATTACTTGTCCTGAGTGTCATAGCAATAGTATCCGCTATTATGGAACAGGGACGCAAAAAGCTTTTGAT
GAATTGCAAGGGGTTATTCCAGAAGCTAAGATCTTGAGAATGGATGTTGACACCACACGAAAAAAAAGAAGTCACAAAAC
CATACTAGATAGTTTTGGTCGCCAGGAAGCAGATATTTTGTTAGGGACGCAAATGATTGCCAAAGGACTGGATTTTCCAA
ATGTGACTTTAGTTGGGGTACTAAATGCAGATACCTCCTTAAATTTGCCAGATTTTCGAGCGTCAGAAAAAACGTTTCAA
TTGTTAACACAGGTTGCTGGTCGGGCAGGCCGTGCCCATAAGCCAGGGGAAGTCTTGATTCAAACTTATAATCCCGATCA
CTATGCAATTCAGTTGGCTAAACAACAAGATTTTGAAGCCTTTTACCGTTATGAAATGGCGATTCGCCGTCAGATGGCTT
ATCCCCCTTACTACTTTACAGTGGGTATTACGCTTTCGCATCGTTTAGAAGCATCGGTTGTAAAAAAAGCTTATCAAGTT
ACAGAACTGCTAAAGAGTCACTTATCGAATAATATTAAAATATTAGGACCGACTCCTAAGCCAATTGCTAGAACACACAA
TTTATACCACTATCAAATTTTGTTAAAATACCGTTTTGAAGATAACCTAGAAGAGACGCTCAATCGTATTCTTGATTGGA
GTCAAGAAGCAGATAATCGTCATTTAAAACTAATTATTGATTGTGAACCGCAACAATTTTTATAA

Upstream 100 bases:

>100_bases
GCCAAAGAAAAAGAAGAAGAAGGAGAAAAAATCTAAGGTTTCAGAAATCTTAGATTTTTTCATATTTAGTACGATGTCCA
TGACAGAAAAGGAGAAGCAA

Downstream 100 bases:

>100_bases
GGTAAATCACTAAATCTAGGATGATCAGTTATGATATAATAAAAATACAAAGAGGAAAGTCATGATAAAATTATTATTTA
TGGGAACTCCCCAATTTTCA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 794; Mature: 794

Protein sequence:

>794_residues
MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGKGNRLLQGFIIGFGQEDSSSLKLIQTVLDPEPVLNQE
QLTLADQLRKTVFSYKITLLKAMIPNLLNSNYDKVLRPESGLKKPDRDFLFEGQPSVLYSTLDREKGKIALKGIQAGHIT
VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQAFSRDVVTYFVTNHLIRIDERPI
DRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKPSKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQ
MTSRFISRFGKQVAIMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESNPRYHAREV
ALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEVTIVDFRDYIGQQAVSNFTPYLIDKIKERLV
KKEQVVLMLNRRGYSSFVMCRDCGYVDKCPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFD
ELQGVIPEAKILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRASEKTFQ
LLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKQQDFEAFYRYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQV
TELLKSHLSNNIKILGPTPKPIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL

Sequences:

>Translated_794_residues
MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGKGNRLLQGFIIGFGQEDSSSLKLIQTVLDPEPVLNQE
QLTLADQLRKTVFSYKITLLKAMIPNLLNSNYDKVLRPESGLKKPDRDFLFEGQPSVLYSTLDREKGKIALKGIQAGHIT
VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQAFSRDVVTYFVTNHLIRIDERPI
DRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKPSKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQ
MTSRFISRFGKQVAIMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESNPRYHAREV
ALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEVTIVDFRDYIGQQAVSNFTPYLIDKIKERLV
KKEQVVLMLNRRGYSSFVMCRDCGYVDKCPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFD
ELQGVIPEAKILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRASEKTFQ
LLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKQQDFEAFYRYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQV
TELLKSHLSNNIKILGPTPKPIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL
>Mature_794_residues
MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGKGNRLLQGFIIGFGQEDSSSLKLIQTVLDPEPVLNQE
QLTLADQLRKTVFSYKITLLKAMIPNLLNSNYDKVLRPESGLKKPDRDFLFEGQPSVLYSTLDREKGKIALKGIQAGHIT
VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQAFSRDVVTYFVTNHLIRIDERPI
DRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKPSKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQ
MTSRFISRFGKQVAIMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESNPRYHAREV
ALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEVTIVDFRDYIGQQAVSNFTPYLIDKIKERLV
KKEQVVLMLNRRGYSSFVMCRDCGYVDKCPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFD
ELQGVIPEAKILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRASEKTFQ
LLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKQQDFEAFYRYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQV
TELLKSHLSNNIKILGPTPKPIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=794, Percent_Identity=34.3828715365239, Blast_Score=423, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 90286; Mature: 90286

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGKGNRLLQGFIIGFGQED
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCEEEEEEEEECCCC
SSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKAMIPNLLNSNYDKVLRPES
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCC
GLKKPDRDFLFEGQPSVLYSTLDREKGKIALKGIQAGHITVSYLAKDKKNLKTEKYYHVD
CCCCCCCCCEECCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCHHCCCCCEEEEEE
LDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQAFSRDVVTYFVTNHLIRIDERPI
HHHEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCC
DRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKPSKPFLIEGITGSGKTEVYLHIIEA
CCCHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHH
VLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVAIMHSGLSDGEKFDEWRKIKTGQAKVV
HHCCCCEEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEEE
VGARSAIFSPLERIGAIIIDEEHESTYKQESNPRYHAREVALLRAKHHQAVVVMGSATPS
EECHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
IESRARASKGVYHFIQLTQRANPLAKIPEVTIVDFRDYIGQQAVSNFTPYLIDKIKERLV
HHHHHHHHCCHHHHHHHHHCCCCHHHCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHH
KKEQVVLMLNRRGYSSFVMCRDCGYVDKCPNCDISLTLHMDTKTMNCHYCGFQKPIPITC
CCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCC
PECHSNSIRYYGTGTQKAFDELQGVIPEAKILRMDVDTTRKKRSHKTILDSFGRQEADIL
CCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHH
LGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLI
HHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEE
QTYNPDHYAIQLAKQQDFEAFYRYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQV
EEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHH
TELLKSHLSNNIKILGPTPKPIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNR
HHHHHHHHCCCEEEECCCCCCHHHCCCEEEEEEEEEEECCCCHHHHHHHHHCCCCCCCCC
HLKLIIDCEPQQFL
EEEEEEECCCCCCC
>Mature Secondary Structure
MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGKGNRLLQGFIIGFGQED
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCEEEEEEEEECCCC
SSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKAMIPNLLNSNYDKVLRPES
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCC
GLKKPDRDFLFEGQPSVLYSTLDREKGKIALKGIQAGHITVSYLAKDKKNLKTEKYYHVD
CCCCCCCCCEECCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCHHCCCCCEEEEEE
LDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQAFSRDVVTYFVTNHLIRIDERPI
HHHEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCC
DRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKPSKPFLIEGITGSGKTEVYLHIIEA
CCCHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHH
VLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVAIMHSGLSDGEKFDEWRKIKTGQAKVV
HHCCCCEEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEEE
VGARSAIFSPLERIGAIIIDEEHESTYKQESNPRYHAREVALLRAKHHQAVVVMGSATPS
EECHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
IESRARASKGVYHFIQLTQRANPLAKIPEVTIVDFRDYIGQQAVSNFTPYLIDKIKERLV
HHHHHHHHCCHHHHHHHHHCCCCHHHCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHH
KKEQVVLMLNRRGYSSFVMCRDCGYVDKCPNCDISLTLHMDTKTMNCHYCGFQKPIPITC
CCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCC
PECHSNSIRYYGTGTQKAFDELQGVIPEAKILRMDVDTTRKKRSHKTILDSFGRQEADIL
CCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHH
LGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLI
HHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEE
QTYNPDHYAIQLAKQQDFEAFYRYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQV
EEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHH
TELLKSHLSNNIKILGPTPKPIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNR
HHHHHHHHCCCEEEECCCCCCHHHCCCEEEEEEEEEEECCCCHHHHHHHHHCCCCCCCCC
HLKLIIDCEPQQFL
EEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]