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The map label for this gene is ddl [H]

Identifier: 19746380

GI number: 19746380

Start: 1197644

End: 1198690

Strand: Reverse

Name: ddl [H]

Synonym: spyM18_1431

Alternate gene names: 19746380

Gene position: 1198690-1197644 (Counterclockwise)

Preceding gene: 19746381

Following gene: 19746379

Centisome position: 63.25

GC content: 39.35

Gene sequence:

>1047_bases
ATGTCCAAACAAACGTTAGTTCTATTATACGGAGGCCGCTCAGCAGAACGTGAAGTCTCAGTGCTTTCAGCAGAGAGTGT
GATGCGAGCAGTCAACTATGATAAGTTCTTAGTAAAAACTTATTTTATTACTCAGATGGGCCAATTTATCAGAACTCAGC
AATTTTCAGAAAAGCCATCGGAATCAGAACGCTTGATGACAAATGAGACGATAGAGCTAACTCAAAAAATAAAACCAAGT
GATATCTATGAAGAAGGTGCAGTTGTATTTCCTGTATTACATGGACCTATGGGAGAAGATGGCTCTATCCAAGGTTTCTT
AGAAGTGCTTAGAATGCCATATATAGGGACTAATGTCATGTCTTCTAGCATAGCTATGGATAAAATTACTACTAAACGTG
TCTTAGAATCAATTGGAATTCCACAAGTTGCCTATACTGTTTATATTGACGGACAAGATTTGGAGGCTTGTTTAGTTGAA
ACTTTAGCAAGACTTACTTTTCCTATCTTCGTAAAACCAGCTAATATGGGTTCTTCTGTGGGGATTTCCAAAGCCCAAAC
AAAAGTAGAGTTAAGAAAAGCGATTCAGTTAGCCCTGACCTATGACAGTCGTGTTTTGATTGAACAAGGCGTAGTTGCCA
GAGAAATTGAAGTTGGTCTCCTTGGAAATGATAAGGTTAAATCAACTCTACCAGGAGAAGTAATCAAGGACGTTGATTTT
TACGACTACCAAGCAAAATATGTTGATAATAAGATTACGATGGCCATTCCAGCAGATGTAGATCAGAGTATTGTGACAGA
AATGCGTTCTTATGCTGAAGTAGCCTTTAAAGCCTTAGGTGGTTGTGGGTTGTCCCGTTGTGACTTTTTCTTGACCCAAG
ACGGACAGGTTTACCTTAACGAATTAAACACCATGCCAGGCTTTACCCAATGGTCTATGTATCCACTCTTATGGGAAAAT
ATGGGATTGGCTTATCCAGATTTGATTGAAGAGCTGGTGACACTGGCACAAGAAATGTTTGACCAGCGTGAGAGCCATCT
GATTTAA

Upstream 100 bases:

>100_bases
TGATCATGTTGGTGCCTTTACTCGCAAAGAGAGGTTTTGAAAAGGCTACATCTTTTTGTTATAATAAAAAAACATTCAAT
TCAGATAAGAAAGGTTAGCT

Downstream 100 bases:

>100_bases
ACCTATGAGATGAATAAACATTGACTTGGATGGAACGGCAAACATCGTCAAGATGTGACGTTCGTCCTTTTTTTGTTGCT
CTTTTAGGAAGAATAGTAGA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFITQMGQFIRTQQFSEKPSESERLMTNETIELTQKIKPS
DIYEEGAVVFPVLHGPMGEDGSIQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEVGLLGNDKVKSTLPGEVIKDVDF
YDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAFKALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWEN
MGLAYPDLIEELVTLAQEMFDQRESHLI

Sequences:

>Translated_348_residues
MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFITQMGQFIRTQQFSEKPSESERLMTNETIELTQKIKPS
DIYEEGAVVFPVLHGPMGEDGSIQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEVGLLGNDKVKSTLPGEVIKDVDF
YDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAFKALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWEN
MGLAYPDLIEELVTLAQEMFDQRESHLI
>Mature_347_residues
SKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFITQMGQFIRTQQFSEKPSESERLMTNETIELTQKIKPSD
IYEEGAVVFPVLHGPMGEDGSIQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVET
LARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEVGLLGNDKVKSTLPGEVIKDVDFY
DYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAFKALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENM
GLAYPDLIEELVTLAQEMFDQRESHLI

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=360, Percent_Identity=35.8333333333333, Blast_Score=244, Evalue=8e-66,
Organism=Escherichia coli, GI1786280, Length=340, Percent_Identity=32.0588235294118, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 38997; Mature: 38866

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFITQMGQFIRTQQFSEKPS
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
ESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGSIQGFLEVLRMPYIGTNVM
CHHHHHCCHHHHHHHCCCCCCHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHH
SSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVETLARLTFPIFVKPANMGSSV
HHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCC
GISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEVGLLGNDKVKSTLPGEVIKDVDF
CCHHHHHHHHHHHHHHHHEECCCEEHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHCCCC
YDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAFKALGGCGLSRCDFFLTQDGQVYLN
CCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHEE
ELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVTLAQEMFDQRESHLI
HHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFITQMGQFIRTQQFSEKPS
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
ESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGSIQGFLEVLRMPYIGTNVM
CHHHHHCCHHHHHHHCCCCCCHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHH
SSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVETLARLTFPIFVKPANMGSSV
HHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCC
GISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEVGLLGNDKVKSTLPGEVIKDVDF
CCHHHHHHHHHHHHHHHHEECCCEEHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHCCCC
YDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAFKALGGCGLSRCDFFLTQDGQVYLN
CCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHEE
ELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVTLAQEMFDQRESHLI
HHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]