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The map label for this gene is murF [H]

Identifier: 19746379

GI number: 19746379

Start: 1196054

End: 1197445

Strand: Reverse

Name: murF [H]

Synonym: spyM18_1429

Alternate gene names: 19746379

Gene position: 1197445-1196054 (Counterclockwise)

Preceding gene: 19746380

Following gene: 19746378

Centisome position: 63.19

GC content: 39.22

Gene sequence:

>1392_bases
ATGAAATTAACCTTACACGAAGTGGCTAAAATAGTTGACGCCCAAAATAATGTGTCTGACTTAGACGACGTTCCTCTGCA
TCACATTGAATTTGATAGCCGAAAGATTACAAAAGGAGACCTCTTTTTACCCTTAAAAGGTCAAAGGGATGGTCATGAGT
TTATTGATTTAGCCTTTCAAAATGGAGCTGTCGCTACTTTTTCAGAAAAAGAATTGTCGGGGAAACCTCACCTTCTTGTT
GAAGACTGCCTAAAAGCTTTTCAAAAGCTAGCCCATTATTATATTGATAAGATGCGCGTGGATGTTATTGCTGTGACAGG
CTCAAATGGTAAAACTTCCACCAAAGATATGATAGGAGCTGTCCTATCAACCACTTACAAAACTTACAAAACGCAAGGAA
ATTACAATAATGAGATTGGTTTGCCTTACACCGTTCTCCACATGCCTGACGATACGGAAAAAATTGTTCTTGAAATGGGA
CAAGATCACATGGGAGATATTCGTCTTCTGTCTGAAATTGCAAGGCCTCGTATTGCAGTTTTAACCTTAGTCGGCGAAGC
TCATTTAGAATATTTTGGAAGCCGAGACAAAATAGCTCAAGGCAAAATGCAGATTGTTGACGGCATGAATTCAGATGGGA
TTTTAATTGCTCCGGGAGATCCGATTATTGACCCATATCTTCCAGAAAATCAGATGGTTATCCGTTTTGGAAATCAGCAG
GAAATTGACGTTACTGGTATTCAAGAAGACAAAGATAGCTTAACGTTTACAACAAATGTGTTAGCAACACCTGTGTCCCT
ACCTCTACCAGGAAAATACAACGCGACCAATGCTATGGTAGCAGCCTATGTAGGAAAACTATTGGCAGTAACAGACGAGG
ATATTATAGCTGCTTTACAGACTGTGACGTTGACAGGTAATCGTACGGAGTGGAAAAAAGCAGCCAATGGTGCTGATATT
TTATCAGATGTCTACAATGCCAACCCAACAGCCATGCGTCTGATTTTAGAAACATTTGCTAACATAGCAAAGAACCCCGG
AGGAAAAAAGATTGCAGTGCTAGCAGATATGAAAGAACTTGGAAAGGATTCAGTGATACTTCATTCTCAGTTAATTGACA
GCTTAACATCAGGTAATATTGATCAGCTTGTTTTTTATGGGGATCATATTAAAGAATTAGCACGGTTGGCAAGTCAGGTT
TATCCTGCTGAGCAAGTACATTATTTTTTAAAAACTGAACAAGAAGACCAGTTTGAAGCCATGGCACAATATGTTCAAAA
CATTCTTAATCCCTTTGATCAAATTCTTTTAAAAGGCAGCCACTCGATGTCGCTTGAAAAGTTAGTGGATCGATTAGTGA
CTGACAAGAATTGTGACAGCTATCAGCCTTGA

Upstream 100 bases:

>100_bases
GAGCCATTAAGGGAGTTTGACATTTTAGAAATTTTTCTAATGTTTTGTGCTATAATAGATAGGACATCGTAAATACTAGT
GTTCTTAGAAAGAAAACAAA

Downstream 100 bases:

>100_bases
TTTTTATAAAACTTTCAGAGAAAGGCTTGTTCTCTCATGAAGATTAAGGTATAATGAAAAAATATAGAAAGGGACGATTA
TGGATTTTTTTGCAATTGAC

Product: D-Ala-D-Ala adding enzyme

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLPLKGQRDGHEFIDLAFQNGAVATFSEKELSGKPHLLV
EDCLKAFQKLAHYYIDKMRVDVIAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
QDHMGDIRLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAPGDPIIDPYLPENQMVIRFGNQQ
EIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNATNAMVAAYVGKLLAVTDEDIIAALQTVTLTGNRTEWKKAANGADI
LSDVYNANPTAMRLILETFANIAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKELARLASQV
YPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVDRLVTDKNCDSYQP

Sequences:

>Translated_463_residues
MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLPLKGQRDGHEFIDLAFQNGAVATFSEKELSGKPHLLV
EDCLKAFQKLAHYYIDKMRVDVIAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
QDHMGDIRLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAPGDPIIDPYLPENQMVIRFGNQQ
EIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNATNAMVAAYVGKLLAVTDEDIIAALQTVTLTGNRTEWKKAANGADI
LSDVYNANPTAMRLILETFANIAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKELARLASQV
YPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVDRLVTDKNCDSYQP
>Mature_463_residues
MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLPLKGQRDGHEFIDLAFQNGAVATFSEKELSGKPHLLV
EDCLKAFQKLAHYYIDKMRVDVIAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
QDHMGDIRLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAPGDPIIDPYLPENQMVIRFGNQQ
EIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNATNAMVAAYVGKLLAVTDEDIIAALQTVTLTGNRTEWKKAANGADI
LSDVYNANPTAMRLILETFANIAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKELARLASQV
YPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVDRLVTDKNCDSYQP

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=448, Percent_Identity=32.1428571428571, Blast_Score=178, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51281; Mature: 51281

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLPLKGQRDGHEFIDLAFQ
CCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEECCCCEEEEECCCCCCCHHHEEEEC
NGAVATFSEKELSGKPHLLVEDCLKAFQKLAHYYIDKMRVDVIAVTGSNGKTSTKDMIGA
CCEEEEECHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHH
VLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMGQDHMGDIRLLSEIARPRIAV
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEE
LTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAPGDPIIDPYLPENQMVIRFGNQQ
EEEHHHHHHHHCCCCCHHHCCCHHHHCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCC
EIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNATNAMVAAYVGKLLAVTDEDIIAALQ
EEEEECCCCCCCCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
TVTLTGNRTEWKKAANGADILSDVYNANPTAMRLILETFANIAKNPGGKKIAVLADMKEL
HEEECCCCHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHC
GKDSVILHSQLIDSLTSGNIDQLVFYGDHIKELARLASQVYPAEQVHYFLKTEQEDQFEA
CCCCEEHHHHHHHHHHCCCHHEEEEECHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHH
MAQYVQNILNPFDQILLKGSHSMSLEKLVDRLVTDKNCDSYQP
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLPLKGQRDGHEFIDLAFQ
CCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEECCCCEEEEECCCCCCCHHHEEEEC
NGAVATFSEKELSGKPHLLVEDCLKAFQKLAHYYIDKMRVDVIAVTGSNGKTSTKDMIGA
CCEEEEECHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHH
VLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMGQDHMGDIRLLSEIARPRIAV
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEE
LTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAPGDPIIDPYLPENQMVIRFGNQQ
EEEHHHHHHHHCCCCCHHHCCCHHHHCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCC
EIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNATNAMVAAYVGKLLAVTDEDIIAALQ
EEEEECCCCCCCCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
TVTLTGNRTEWKKAANGADILSDVYNANPTAMRLILETFANIAKNPGGKKIAVLADMKEL
HEEECCCCHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHC
GKDSVILHSQLIDSLTSGNIDQLVFYGDHIKELARLASQVYPAEQVHYFLKTEQEDQFEA
CCCCEEHHHHHHHHHHCCCHHEEEEECHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHH
MAQYVQNILNPFDQILLKGSHSMSLEKLVDRLVTDKNCDSYQP
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]