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The map label for this gene is glpK

Identifier: 18978376

GI number: 18978376

Start: 1852917

End: 1854389

Strand: Direct

Name: glpK

Synonym: PF2004

Alternate gene names: 18978376

Gene position: 1852917-1854389 (Clockwise)

Preceding gene: 18978375

Following gene: 18978377

Centisome position: 97.1

GC content: 43.65

Gene sequence:

>1473_bases
ATGGACCGCTATATCCTCTCTTTAGATGAGGGCACAACTTCCGCCAGGGCAATAATATTTGATAAGAACAGCAATGTTCT
AGGAATTGGGCAGTATGAATTCCCTCAATACTATCCCAAGCCAGGGTGGGTAGAGCATAATCCAGAGGAAATTTGGGAGG
CTCAAGTGAAAGCAATAAAGACTGCGATAGAGAAAGCCAAGATAGAGGCAAATCAAATAGCTGCTATTGGAGTCACAAAC
CAGCGTGAAACTACAGTTATTTGGAAAAAAGATGGGAAACCCGTTTACAATGCCATTGTGTGGCAGTGCAGAAGAACTGC
TGAAATGGTTGAGGAAATTAAGAGAGAATATAGTGATGTCATTAAAGAAAAGACAGGTCTTGTTCCAGATGCCTACTTTT
CAGCCTCAAAGATAGCATGGCTCTTGGACAATGTCCCTGGGCTTAGAGAAAAAGCGGAAAAAGGAGAGGTTATGTTCGGT
ACAATTGACACCTTTCTAATTTACAGGCTTACGGGAGAGCACGTAACGGACTATTCAAATGCCTCCAGAACAATGTTGTT
CAACATTAATAAGCTGGAGTGGGATGAAGAGCTTTTAGAGATCTTTAAGATTCCCGATGGAATTTTACCTGATGTAAAAG
AATCTAGTGAAATCTATGGCTATACAAAAATTCTTGGCCCAGAAATTCCAGTTAGTGGGGATGCTGGAGATCAACAGGCT
GCTCTCTTTGGCCAAGCAGCATTTGAAGAGGGAATGGTAAAGGCCACTTACGGGACTGGAAACTTCATATTGGCGAATAC
AGGAAGAATGCTTCTTTATTCAGATAACCTCCTCACAACGGTTGCATGGGGGATTAATGGGAAGGTAAATTATGCCCTGG
AAGGCAGTATCTTCATCACGGGAGCAGCTGTTCAGTGGTTGAGAGACGGGATTAAGATAATCAGCAATGCCGCTGAGACT
GAAGAATTAGCTACTAAGTTAGAGAGCAACGAGGGAGTCTACTTCGTTCCAGCATTTGTAGGATTGGGAGCTCCCTACTG
GGATCAGTTCGCTAGGGGGTTGATAATTGGAATAACAAGGGGAACTGGGAGAGAACATCTTGCAAGGGCAACCTTAGAGG
CCATAGCTTACCTCACAAGGGATGTAATTGAAGAGATGGAAAAGCTGATACAGATAAAGGAGCTTCGCGTGGATGGTGGA
GCCACTGTTAACAACTTCCTCATGCAGTTCCAGGCTGATATCCTCAATAAGAAAGTTATTAGACCTGTAGTTAAAGAGAC
CACTGCCCTTGGAGCTGCCTACTTAGCTGGTTTGGCCGTAGACTATTGGGAAAGCATGGAGGAGATATCACAACTCTGGA
GAGCTGAGAGAGTATTTGAACCTCAGATGGATGAAAAAACTAGAGAAAAACTATATAGGGGATGGAAAGAGGCCGTTAAG
AGAGCTTTAGGTTGGGCCAAAGTTGTGAGTTGA

Upstream 100 bases:

>100_bases
TAGCTAATGATGTTGAGAGAATGGTATCCTACCTTTCTTCCCTGGGAATAAGGTAAAAATAATAACCCTTCTTTTTCATA
ACTCTAAATCAGGTGGTTAA

Downstream 100 bases:

>100_bases
GGAGGGGGATAAATGATTAAGGTTGCGATAATTGGAGCGGGAATAACGGGAGCTAGTATAGCAAGAGTTCTAAGCAGATA
TGAAAACCTCGAGGTTCATC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MDRYILSLDEGTTSARAIIFDKNSNVLGIGQYEFPQYYPKPGWVEHNPEEIWEAQVKAIKTAIEKAKIEANQIAAIGVTN
QRETTVIWKKDGKPVYNAIVWQCRRTAEMVEEIKREYSDVIKEKTGLVPDAYFSASKIAWLLDNVPGLREKAEKGEVMFG
TIDTFLIYRLTGEHVTDYSNASRTMLFNINKLEWDEELLEIFKIPDGILPDVKESSEIYGYTKILGPEIPVSGDAGDQQA
ALFGQAAFEEGMVKATYGTGNFILANTGRMLLYSDNLLTTVAWGINGKVNYALEGSIFITGAAVQWLRDGIKIISNAAET
EELATKLESNEGVYFVPAFVGLGAPYWDQFARGLIIGITRGTGREHLARATLEAIAYLTRDVIEEMEKLIQIKELRVDGG
ATVNNFLMQFQADILNKKVIRPVVKETTALGAAYLAGLAVDYWESMEEISQLWRAERVFEPQMDEKTREKLYRGWKEAVK
RALGWAKVVS

Sequences:

>Translated_490_residues
MDRYILSLDEGTTSARAIIFDKNSNVLGIGQYEFPQYYPKPGWVEHNPEEIWEAQVKAIKTAIEKAKIEANQIAAIGVTN
QRETTVIWKKDGKPVYNAIVWQCRRTAEMVEEIKREYSDVIKEKTGLVPDAYFSASKIAWLLDNVPGLREKAEKGEVMFG
TIDTFLIYRLTGEHVTDYSNASRTMLFNINKLEWDEELLEIFKIPDGILPDVKESSEIYGYTKILGPEIPVSGDAGDQQA
ALFGQAAFEEGMVKATYGTGNFILANTGRMLLYSDNLLTTVAWGINGKVNYALEGSIFITGAAVQWLRDGIKIISNAAET
EELATKLESNEGVYFVPAFVGLGAPYWDQFARGLIIGITRGTGREHLARATLEAIAYLTRDVIEEMEKLIQIKELRVDGG
ATVNNFLMQFQADILNKKVIRPVVKETTALGAAYLAGLAVDYWESMEEISQLWRAERVFEPQMDEKTREKLYRGWKEAVK
RALGWAKVVS
>Mature_490_residues
MDRYILSLDEGTTSARAIIFDKNSNVLGIGQYEFPQYYPKPGWVEHNPEEIWEAQVKAIKTAIEKAKIEANQIAAIGVTN
QRETTVIWKKDGKPVYNAIVWQCRRTAEMVEEIKREYSDVIKEKTGLVPDAYFSASKIAWLLDNVPGLREKAEKGEVMFG
TIDTFLIYRLTGEHVTDYSNASRTMLFNINKLEWDEELLEIFKIPDGILPDVKESSEIYGYTKILGPEIPVSGDAGDQQA
ALFGQAAFEEGMVKATYGTGNFILANTGRMLLYSDNLLTTVAWGINGKVNYALEGSIFITGAAVQWLRDGIKIISNAAET
EELATKLESNEGVYFVPAFVGLGAPYWDQFARGLIIGITRGTGREHLARATLEAIAYLTRDVIEEMEKLIQIKELRVDGG
ATVNNFLMQFQADILNKKVIRPVVKETTALGAAYLAGLAVDYWESMEEISQLWRAERVFEPQMDEKTREKLYRGWKEAVK
RALGWAKVVS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI41393575, Length=497, Percent_Identity=46.2776659959759, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI189409120, Length=505, Percent_Identity=45.9405940594059, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI4504007, Length=505, Percent_Identity=45.9405940594059, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI42794763, Length=511, Percent_Identity=45.4011741682975, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI88196792, Length=507, Percent_Identity=36.094674556213, Blast_Score=321, Evalue=1e-87,
Organism=Escherichia coli, GI1790361, Length=493, Percent_Identity=56.9979716024341, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1787789, Length=518, Percent_Identity=25.8687258687259, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1789987, Length=501, Percent_Identity=24.5508982035928, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1790005, Length=496, Percent_Identity=25, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789168, Length=492, Percent_Identity=25.4065040650407, Blast_Score=104, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17535599, Length=501, Percent_Identity=42.1157684630739, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71986140, Length=520, Percent_Identity=26.7307692307692, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71986147, Length=307, Percent_Identity=32.8990228013029, Blast_Score=148, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6321755, Length=551, Percent_Identity=33.5753176043557, Blast_Score=284, Evalue=2e-77,
Organism=Drosophila melanogaster, GI17864214, Length=506, Percent_Identity=42.8853754940711, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24654573, Length=432, Percent_Identity=44.6759259259259, Blast_Score=354, Evalue=9e-98,
Organism=Drosophila melanogaster, GI24655555, Length=502, Percent_Identity=37.4501992031873, Blast_Score=326, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24655560, Length=502, Percent_Identity=37.4501992031873, Blast_Score=326, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24655570, Length=502, Percent_Identity=37.4501992031873, Blast_Score=326, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24655574, Length=502, Percent_Identity=37.4501992031873, Blast_Score=326, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24655565, Length=502, Percent_Identity=37.4501992031873, Blast_Score=326, Evalue=3e-89,
Organism=Drosophila melanogaster, GI19922058, Length=525, Percent_Identity=33.3333333333333, Blast_Score=268, Evalue=8e-72,
Organism=Drosophila melanogaster, GI24652871, Length=492, Percent_Identity=33.5365853658537, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24656367, Length=513, Percent_Identity=28.6549707602339, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI21357867, Length=525, Percent_Identity=27.6190476190476, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=30.0859598853868, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=28.2758620689655, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_PYRFU (Q8TZI8)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579733.1
- ProteinModelPortal:   Q8TZI8
- SMR:   Q8TZI8
- EnsemblBacteria:   EBPYRT00000004960
- GeneID:   1469889
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF2004
- NMPDR:   fig|186497.1.peg.2059
- GeneTree:   EBGT00050000022271
- HOGENOM:   HBG511469
- OMA:   ATKLESN
- ProtClustDB:   PRK00047
- BRENDA:   2.7.1.30
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55046; Mature: 55046

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 132-132 BINDING 237-237 BINDING 259-259 BINDING 301-301

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRYILSLDEGTTSARAIIFDKNSNVLGIGQYEFPQYYPKPGWVEHNPEEIWEAQVKAIK
CCCEEEEECCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TAIEKAKIEANQIAAIGVTNQRETTVIWKKDGKPVYNAIVWQCRRTAEMVEEIKREYSDV
HHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKEKTGLVPDAYFSASKIAWLLDNVPGLREKAEKGEVMFGTIDTFLIYRLTGEHVTDYSN
HHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEHHHEEEEEECCCHHCCCCC
ASRTMLFNINKLEWDEELLEIFKIPDGILPDVKESSEIYGYTKILGPEIPVSGDAGDQQA
CCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHEEEHHHCCCCCCCCCCCCCCHH
ALFGQAAFEEGMVKATYGTGNFILANTGRMLLYSDNLLTTVAWGINGKVNYALEGSIFIT
HHHHHHHHHCCCEEEEECCCCEEEEECCEEEEEECCEEEEEEECCCCEEEEEEECCEEEE
GAAVQWLRDGIKIISNAAETEELATKLESNEGVYFVPAFVGLGAPYWDQFARGLIIGITR
HHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHCCCEEEEEEC
GTGREHLARATLEAIAYLTRDVIEEMEKLIQIKELRVDGGATVNNFLMQFQADILNKKVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RPVVKETTALGAAYLAGLAVDYWESMEEISQLWRAERVFEPQMDEKTREKLYRGWKEAVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RALGWAKVVS
HHHHHHHHCC
>Mature Secondary Structure
MDRYILSLDEGTTSARAIIFDKNSNVLGIGQYEFPQYYPKPGWVEHNPEEIWEAQVKAIK
CCCEEEEECCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TAIEKAKIEANQIAAIGVTNQRETTVIWKKDGKPVYNAIVWQCRRTAEMVEEIKREYSDV
HHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKEKTGLVPDAYFSASKIAWLLDNVPGLREKAEKGEVMFGTIDTFLIYRLTGEHVTDYSN
HHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEHHHEEEEEECCCHHCCCCC
ASRTMLFNINKLEWDEELLEIFKIPDGILPDVKESSEIYGYTKILGPEIPVSGDAGDQQA
CCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHEEEHHHCCCCCCCCCCCCCCHH
ALFGQAAFEEGMVKATYGTGNFILANTGRMLLYSDNLLTTVAWGINGKVNYALEGSIFIT
HHHHHHHHHCCCEEEEECCCCEEEEECCEEEEEECCEEEEEEECCCCEEEEEEECCEEEE
GAAVQWLRDGIKIISNAAETEELATKLESNEGVYFVPAFVGLGAPYWDQFARGLIIGITR
HHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHCCCEEEEEEC
GTGREHLARATLEAIAYLTRDVIEEMEKLIQIKELRVDGGATVNNFLMQFQADILNKKVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RPVVKETTALGAAYLAGLAVDYWESMEEISQLWRAERVFEPQMDEKTREKLYRGWKEAVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RALGWAKVVS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA