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The map label for this gene is ksgA

Identifier: 18978235

GI number: 18978235

Start: 1719412

End: 1720233

Strand: Direct

Name: ksgA

Synonym: PF1863

Alternate gene names: 18978235

Gene position: 1719412-1720233 (Clockwise)

Preceding gene: 18978234

Following gene: 18978237

Centisome position: 90.1

GC content: 35.89

Gene sequence:

>822_bases
ATGAGAGACAAACTTTTCTATTTCCTCTCCAAATACAATTTTTCTCCTAGAGACAAAATAGGCCAGAATTTCCTAATCAT
GAGAGATGTTATCATAAAAGCCGTTGAAACATCTGAAATCAAGAAAAGCGATGTAGTTTTAGAAGTAGGTCCAGGGTTCG
GCTTCTTGACAGACGAGTTATCAAAAAGAGCTGGGAAAGTCTATGCAATAGAGCTGGATAAAAGAATCATTGAAATCTTG
GAAAATGAATATAACTGGGAGAACGTTGAAATCATTCAGGGAGATGCTGTAAAAATTGAATGGCCCGAATTCAACAAGGT
TGTTTCTAACATTCCCTATCAGATTTCTTCTCCCTTTACCTTCAAACTGCTGAAGCATGATTTTGAAAAAGCTGTTGTTA
TGTACCAACTGGAATTTGCAAAGAGAATGGTTGCAAAACCTGGAGATAGAAACTATTCAAGACTTTCCCTAATGGTGAAC
GCTTTAGCAAATGCTAAAATTGTAATGAAGATAGGAAGGGGGGCATTCTATCCAAAACCCAAGGTGGATTCTGCATTAGT
TCTTATCGTTCCAAAACCTAAAGATGAGAGAATAGAGTTAAATGAGAACTTAGTAAAAGCTCTGTTTCAGCATAGGAGAA
AGCTAGTGTCAAAAGCCCTCAAGGAGTCATGCCACATGCTTGGAATAAACAAAAAGGAACTAAAGACATTAAAAAACATT
CTAGAAAATGTACCGCATGCAAAGAAAAGAGTGTTTGAACTTACCCCAGAAGAAGTTAAAGAGATTGAAGAATTTCTAAA
AATTCAAGGAATCATTAATTAA

Upstream 100 bases:

>100_bases
ATCCAGTAAAGATGATTGCAAAAAGAATATTAGAAGAGCTGCAAGGAAAAGACAAATACCGACTATTTGTAGGGAGTAAT
AGAATATTCCGTGAATAGAC

Downstream 100 bases:

>100_bases
TAATTTTTTCAAAAAGTTTATCTATATATTATGGATACTGTCAGGATAACTGGGGCATCACCTCTTGAAGCCACTCAGTC
ACATCACCAGGCGGACAACC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MRDKLFYFLSKYNFSPRDKIGQNFLIMRDVIIKAVETSEIKKSDVVLEVGPGFGFLTDELSKRAGKVYAIELDKRIIEIL
ENEYNWENVEIIQGDAVKIEWPEFNKVVSNIPYQISSPFTFKLLKHDFEKAVVMYQLEFAKRMVAKPGDRNYSRLSLMVN
ALANAKIVMKIGRGAFYPKPKVDSALVLIVPKPKDERIELNENLVKALFQHRRKLVSKALKESCHMLGINKKELKTLKNI
LENVPHAKKRVFELTPEEVKEIEEFLKIQGIIN

Sequences:

>Translated_273_residues
MRDKLFYFLSKYNFSPRDKIGQNFLIMRDVIIKAVETSEIKKSDVVLEVGPGFGFLTDELSKRAGKVYAIELDKRIIEIL
ENEYNWENVEIIQGDAVKIEWPEFNKVVSNIPYQISSPFTFKLLKHDFEKAVVMYQLEFAKRMVAKPGDRNYSRLSLMVN
ALANAKIVMKIGRGAFYPKPKVDSALVLIVPKPKDERIELNENLVKALFQHRRKLVSKALKESCHMLGINKKELKTLKNI
LENVPHAKKRVFELTPEEVKEIEEFLKIQGIIN
>Mature_273_residues
MRDKLFYFLSKYNFSPRDKIGQNFLIMRDVIIKAVETSEIKKSDVVLEVGPGFGFLTDELSKRAGKVYAIELDKRIIEIL
ENEYNWENVEIIQGDAVKIEWPEFNKVVSNIPYQISSPFTFKLLKHDFEKAVVMYQLEFAKRMVAKPGDRNYSRLSLMVN
ALANAKIVMKIGRGAFYPKPKVDSALVLIVPKPKDERIELNENLVKALFQHRRKLVSKALKESCHMLGINKKELKTLKNI
LENVPHAKKRVFELTPEEVKEIEEFLKIQGIIN

Specific function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=212, Percent_Identity=42.4528301886792, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI156415992, Length=255, Percent_Identity=28.2352941176471, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1786236, Length=220, Percent_Identity=32.2727272727273, Blast_Score=103, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI25146882, Length=203, Percent_Identity=43.8423645320197, Blast_Score=147, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI25141369, Length=250, Percent_Identity=27.6, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324989, Length=235, Percent_Identity=37.0212765957447, Blast_Score=135, Evalue=7e-33,
Organism=Drosophila melanogaster, GI21358017, Length=229, Percent_Identity=38.4279475982533, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21357273, Length=237, Percent_Identity=27.4261603375527, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PYRFU (Q8TH24)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579592.1
- ProteinModelPortal:   Q8TH24
- SMR:   Q8TH24
- EnsemblBacteria:   EBPYRT00000005092
- GeneID:   1469742
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1863
- NMPDR:   fig|186497.1.peg.1912
- GeneTree:   EBGT00050000023397
- HOGENOM:   HBG319664
- OMA:   DRNYSRL
- ProtClustDB:   PRK14896
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31628; Mature: 31628

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 23-23 BINDING 25-25 BINDING 50-50 BINDING 71-71 BINDING 95-95 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDKLFYFLSKYNFSPRDKIGQNFLIMRDVIIKAVETSEIKKSDVVLEVGPGFGFLTDEL
CCCHHEEHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
SKRAGKVYAIELDKRIIEILENEYNWENVEIIQGDAVKIEWPEFNKVVSNIPYQISSPFT
HHHCCCEEEEEHHHHHHHHHHCCCCCCCEEEEECCEEEEECCHHHHHHHCCCCCCCCCCH
FKLLKHDFEKAVVMYQLEFAKRMVAKPGDRNYSRLSLMVNALANAKIVMKIGRGAFYPKP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCC
KVDSALVLIVPKPKDERIELNENLVKALFQHRRKLVSKALKESCHMLGINKKELKTLKNI
CCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LENVPHAKKRVFELTPEEVKEIEEFLKIQGIIN
HHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRDKLFYFLSKYNFSPRDKIGQNFLIMRDVIIKAVETSEIKKSDVVLEVGPGFGFLTDEL
CCCHHEEHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
SKRAGKVYAIELDKRIIEILENEYNWENVEIIQGDAVKIEWPEFNKVVSNIPYQISSPFT
HHHCCCEEEEEHHHHHHHHHHCCCCCCCEEEEECCEEEEECCHHHHHHHCCCCCCCCCCH
FKLLKHDFEKAVVMYQLEFAKRMVAKPGDRNYSRLSLMVNALANAKIVMKIGRGAFYPKP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCC
KVDSALVLIVPKPKDERIELNENLVKALFQHRRKLVSKALKESCHMLGINKKELKTLKNI
CCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LENVPHAKKRVFELTPEEVKEIEEFLKIQGIIN
HHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Dimethylation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA