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The map label for this gene is lysX [H]

Identifier: 18978054

GI number: 18978054

Start: 1563887

End: 1564708

Strand: Direct

Name: lysX [H]

Synonym: PF1682

Alternate gene names: 18978054

Gene position: 1563887-1564708 (Clockwise)

Preceding gene: 18978053

Following gene: 18978055

Centisome position: 81.95

GC content: 39.9

Gene sequence:

>822_bases
ATGAAGATCGGAATTACATACACAATCTTGAGAAAAGAGGAAGTAATGATTAGAGAGAGAGCCAGTGAGTTTGGAGAAGT
TATAATGTTGCACGAGGACGACATGACATTTCCCGCAAAATTTGACTTAGACGTTGTTATAATTAGGAATATAAGCCATT
TTAAGGCCCTATACCTAGCAAAGTTGTTTGAAGATGCTGGAATTCCCGTAGTAAATCCGTTTAATATAATACTTGAAACT
GGAGACAAATTACTTGCAACATTGAAACTTTCAAAGAAAGTAAAGGTTCCCAGGTGGGGAGTTGCAATAAGCGAGAATGG
CGCGAAAAATTTGGGGAAAAAGCTTGGATTTCCATTTGTATCAAAGCCTATCTATGGAAGCTGGGGTAGGTTAGTGACGA
AAGTAAACGATATCGACGCCCTAGAGAGCCTACTTGAACATAAAACCTGGATGAAAAATCCCCTTTACAACATCCATTAC
TTTCAAGAGTTCGTAGAAAAACCTGGAAGAGACATTAGGAGTTATGTGATAGGAGGAGAATTCGTGACTGCTATTTACAG
GTATTCTGATCATTGGATTACAAACACAGCTAGGGGAGGCAAGGCTACCCCCTGTACAGATGAAGATGTCAAAGAAGTCT
CAATAAAAGCTTGGGAGGCATTTGGAGATGGAGCTTTAGCAATAGACATTTTTGAAAGCCCTCAGGGGTTGTTAGTCAAC
GAAGTTAATCCAAATATGGAGTTTAAGAACGCAGCCAGAGTTACTGGAGTTGATATTGCTCGAAAACTCGTTGAATATGC
AGTGGAGGTGGCAAAGAGATGA

Upstream 100 bases:

>100_bases
GTTAGTTTGAACCCCCTAACACTGGAAGAGCTTCCAGAAGTTGAAGAGGACTGGGGAGAGTAGTTTCCCCAGCTTTTATT
TTTTTATGGAGGAGAATCCT

Downstream 100 bases:

>100_bases
TCAAGGCTGCTGTTGTTGGGGCTAGTGGATATATCGGAGGGGAATTAGTTAGGTTACTCGCAATGCATCCAGAAGTTGAG
ATTACTGCAATAACCTCAAG

Product: ribosomal protein s6 modification protein

Products: NA

Alternate protein names: AAA--lysW ligase lysX [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKIGITYTILRKEEVMIRERASEFGEVIMLHEDDMTFPAKFDLDVVIIRNISHFKALYLAKLFEDAGIPVVNPFNIILET
GDKLLATLKLSKKVKVPRWGVAISENGAKNLGKKLGFPFVSKPIYGSWGRLVTKVNDIDALESLLEHKTWMKNPLYNIHY
FQEFVEKPGRDIRSYVIGGEFVTAIYRYSDHWITNTARGGKATPCTDEDVKEVSIKAWEAFGDGALAIDIFESPQGLLVN
EVNPNMEFKNAARVTGVDIARKLVEYAVEVAKR

Sequences:

>Translated_273_residues
MKIGITYTILRKEEVMIRERASEFGEVIMLHEDDMTFPAKFDLDVVIIRNISHFKALYLAKLFEDAGIPVVNPFNIILET
GDKLLATLKLSKKVKVPRWGVAISENGAKNLGKKLGFPFVSKPIYGSWGRLVTKVNDIDALESLLEHKTWMKNPLYNIHY
FQEFVEKPGRDIRSYVIGGEFVTAIYRYSDHWITNTARGGKATPCTDEDVKEVSIKAWEAFGDGALAIDIFESPQGLLVN
EVNPNMEFKNAARVTGVDIARKLVEYAVEVAKR
>Mature_273_residues
MKIGITYTILRKEEVMIRERASEFGEVIMLHEDDMTFPAKFDLDVVIIRNISHFKALYLAKLFEDAGIPVVNPFNIILET
GDKLLATLKLSKKVKVPRWGVAISENGAKNLGKKLGFPFVSKPIYGSWGRLVTKVNDIDALESLLEHKTWMKNPLYNIHY
FQEFVEKPGRDIRSYVIGGEFVTAIYRYSDHWITNTARGGKATPCTDEDVKEVSIKAWEAFGDGALAIDIFESPQGLLVN
EVNPNMEFKNAARVTGVDIARKLVEYAVEVAKR

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of 'Glu-54' of lysW [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1787076, Length=213, Percent_Identity=29.5774647887324, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 30856; Mature: 30856

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGITYTILRKEEVMIRERASEFGEVIMLHEDDMTFPAKFDLDVVIIRNISHFKALYLA
CEEEEEEEEECHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHH
KLFEDAGIPVVNPFNIILETGDKLLATLKLSKKVKVPRWGVAISENGAKNLGKKLGFPFV
HHHHHCCCCEECCHHHEEECCCHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCCCC
SKPIYGSWGRLVTKVNDIDALESLLEHKTWMKNPLYNIHYFQEFVEKPGRDIRSYVIGGE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHCCHH
FVTAIYRYSDHWITNTARGGKATPCTDEDVKEVSIKAWEAFGDGALAIDIFESPQGLLVN
HHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEE
EVNPNMEFKNAARVTGVDIARKLVEYAVEVAKR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIGITYTILRKEEVMIRERASEFGEVIMLHEDDMTFPAKFDLDVVIIRNISHFKALYLA
CEEEEEEEEECHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHH
KLFEDAGIPVVNPFNIILETGDKLLATLKLSKKVKVPRWGVAISENGAKNLGKKLGFPFV
HHHHHCCCCEECCHHHEEECCCHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCCCC
SKPIYGSWGRLVTKVNDIDALESLLEHKTWMKNPLYNIHYFQEFVEKPGRDIRSYVIGGE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHCCHH
FVTAIYRYSDHWITNTARGGKATPCTDEDVKEVSIKAWEAFGDGALAIDIFESPQGLLVN
HHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEE
EVNPNMEFKNAARVTGVDIARKLVEYAVEVAKR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12963379 [H]