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The map label for this gene is leuD

Identifier: 18978052

GI number: 18978052

Start: 1562994

End: 1563488

Strand: Direct

Name: leuD

Synonym: PF1680

Alternate gene names: 18978052

Gene position: 1562994-1563488 (Clockwise)

Preceding gene: 18978051

Following gene: 18978053

Centisome position: 81.91

GC content: 39.8

Gene sequence:

>495_bases
ATGATAACAAGAGGTAGGGTATGGAAATTTGGCGATAACATTTCAACAGATGCAATAACCCCTGGAAGGTATAATCTAAC
AAAAGATCCAAACGAGCTAGCAAAGATAGCATTCATTGAAGAAAGACCAGAGTTTTCCAAAGAAGTTAAACCAGGAGATG
TAGTTGTTGGAGGAAAAAACTTTGGAATAGGATCATCTAGAGAATCCGCAGCCCTAGCACTAAAAGCTGCTGGAGTAGGG
GGAGTAATTGCAAAATCCTTCGGGAGAATATTCTTTAGAAACGCAGTAAACCTGGGACTTCCCCTCTTGATAGGGAATAC
TGACCCACTCTTAGATGGAGAAATTGTTGAGGTTAATTGGAGAAGTGGAGAGGTAAAGAAAGAAGATGGGGAAGTTCTAA
AGTTTAAACCCCTTGACAGTTTTCTTCTCGCTATAGTCGAAGAAGGAGGTATTATTGAATACATTAGAAGGAGGGGAGAT
TTATGGATACAATAG

Upstream 100 bases:

>100_bases
GAAGAATGGGATCTCCCAATGCCCAAATTTACCTTTCTAATCCAATAACTGCAGCTGTAAGCGCCTTGTATGGAGAGATA
ACGAACCCGGAGGGAGCTAT

Downstream 100 bases:

>100_bases
CAGTCATTTGGGAGACTCTAAAAAACTTAGATGAGGATATAGAGGATCTTTGGAACGAAGAAATTAGAGTAAGCACTATA
TTAACTCGCTTTGATATGTA

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MITRGRVWKFGDNISTDAITPGRYNLTKDPNELAKIAFIEERPEFSKEVKPGDVVVGGKNFGIGSSRESAALALKAAGVG
GVIAKSFGRIFFRNAVNLGLPLLIGNTDPLLDGEIVEVNWRSGEVKKEDGEVLKFKPLDSFLLAIVEEGGIIEYIRRRGD
LWIQ

Sequences:

>Translated_164_residues
MITRGRVWKFGDNISTDAITPGRYNLTKDPNELAKIAFIEERPEFSKEVKPGDVVVGGKNFGIGSSRESAALALKAAGVG
GVIAKSFGRIFFRNAVNLGLPLLIGNTDPLLDGEIVEVNWRSGEVKKEDGEVLKFKPLDSFLLAIVEEGGIIEYIRRRGD
LWIQ
>Mature_164_residues
MITRGRVWKFGDNISTDAITPGRYNLTKDPNELAKIAFIEERPEFSKEVKPGDVVVGGKNFGIGSSRESAALALKAAGVG
GVIAKSFGRIFFRNAVNLGLPLLIGNTDPLLDGEIVEVNWRSGEVKKEDGEVLKFKPLDSFLLAIVEEGGIIEYIRRRGD
LWIQ

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Saccharomyces cerevisiae, GI6320440, Length=126, Percent_Identity=43.6507936507937, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD2_PYRFU (Q8U0B9)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579409.1
- ProteinModelPortal:   Q8U0B9
- SMR:   Q8U0B9
- EnsemblBacteria:   EBPYRT00000004172
- GeneID:   1469557
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1680
- NMPDR:   fig|186497.1.peg.1728
- GeneTree:   EBGT00050000022453
- HOGENOM:   HBG304838
- OMA:   TDDILPG
- ProtClustDB:   PRK00439
- BRENDA:   4.2.1.33
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 17988; Mature: 17988

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRGRVWKFGDNISTDAITPGRYNLTKDPNELAKIAFIEERPEFSKEVKPGDVVVGGKN
CCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCHHCCCCCCEEECCCC
FGIGSSRESAALALKAAGVGGVIAKSFGRIFFRNAVNLGLPLLIGNTDPLLDGEIVEVNW
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEE
RSGEVKKEDGEVLKFKPLDSFLLAIVEEGGIIEYIRRRGDLWIQ
CCCCCCCCCCCEEEEECHHHHHHHHHCCCCHHHHHHHCCCEECC
>Mature Secondary Structure
MITRGRVWKFGDNISTDAITPGRYNLTKDPNELAKIAFIEERPEFSKEVKPGDVVVGGKN
CCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCHHCCCCCCEEECCCC
FGIGSSRESAALALKAAGVGGVIAKSFGRIFFRNAVNLGLPLLIGNTDPLLDGEIVEVNW
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEE
RSGEVKKEDGEVLKFKPLDSFLLAIVEEGGIIEYIRRRGDLWIQ
CCCCCCCCCCCEEEEECHHHHHHHHHCCCCHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA