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The map label for this gene is leuC2

Identifier: 18978051

GI number: 18978051

Start: 1561855

End: 1562997

Strand: Direct

Name: leuC2

Synonym: PF1679

Alternate gene names: 18978051

Gene position: 1561855-1562997 (Clockwise)

Preceding gene: 161484707

Following gene: 18978052

Centisome position: 81.85

GC content: 43.04

Gene sequence:

>1143_bases
ATGACATTAATTGAAGAAATTCTCGGTGGAAAATCTGGAGAAAGTGTTGTTAGAAGAGTGGATTTAGTATACGCTCATGA
TGGGACAATGCCCTTAATAATAGAGGCATTCAACAAGGTCTTTGCCACTGTAAGAGCAAGAGCATATATCTTCTTTGACC
ACGTGTATCCAGCGCCTACAGTAAAGATTGCAAACTTGCAAAAAGAGATTAGAGATTTCGCAAAAAGGCATAGAATTCCA
GTAATAGAAGGTCAAGGAATTAGCCATCAACTCGTCGTTGAAATGGGATTAACAGAAAACTCAAAGATAGTTGTGGGAGC
AGATTCCCATACTCCAACTCTCGGAGCACTTGGAGTTTTTGCAGTAGGGATGGGAGCAACGGATGTTGCTGTAATCTTAG
GGCTAGGAAAGACATGGTTTAGAATTCCAGAGAGCGTTGGAGTAATCCTAGAGGGCAATCCTTCAAGATACGTTATGGCA
ACTGATGTGATACTTCATCTCCTCTCACTACTAAAAGACTACGACATGAACTACAGGGCCGTAGAATTCTTCAACGTTCC
TTTTTCCCTTGATGAGCGTCTAACGCTAACGAACTTTGTAGTGGAAGCTAATGCAAAAACGGGAATAATAGGGGAAGAGT
ACACAGGAGATGGGTATGTAAAAGAGCTGGAGATTGAATTAAATTCTCTCAATCCCCTTGTTGCAAAGCCGCATAATCCA
GCAAACGTTGTTCCAGTGGAAGAAGTAGAGGGAACAAAGATAGATCAGGTTTTCATAGGCTCGTGCACAAATGGGAGGTT
TGAACAAATTTCCAAAGCTGCCGAGATTTTAGAGGGAGAGAAAGTAGCAGTTAGAACCATCGTAGGTCCTGCCTCTATGA
ATGTATACAAGAGAATGATAGAGGAGGGAGTAGCAAGAAAACTCATTGAAGCTGGAGCAGTTATTCTTCCCCCAGGATGC
GGGCCTTGCTTAGGGAGGCATATGGGCGTAGTTGGAGATGGGGAGATAGTCCTAAGCACAACAAACAGGAACTTCAGAGG
AAGAATGGGATCTCCCAATGCCCAAATTTACCTTTCTAATCCAATAACTGCAGCTGTAAGCGCCTTGTATGGAGAGATAA
CGAACCCGGAGGGAGCTATATGA

Upstream 100 bases:

>100_bases
AAAACGAAAATATTGTTGAAGAACTTCTAAGAAGAGTAAAAGAAAGCAGAGAACCTTATACCTGGGAAAAATTAATAGAA
GAAGCTAGGAGGTTAGCAAC

Downstream 100 bases:

>100_bases
TAACAAGAGGTAGGGTATGGAAATTTGGCGATAACATTTCAACAGATGCAATAACCCCTGGAAGGTATAATCTAACAAAA
GATCCAAACGAGCTAGCAAA

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTLIEEILGGKSGESVVRRVDLVYAHDGTMPLIIEAFNKVFATVRARAYIFFDHVYPAPTVKIANLQKEIRDFAKRHRIP
VIEGQGISHQLVVEMGLTENSKIVVGADSHTPTLGALGVFAVGMGATDVAVILGLGKTWFRIPESVGVILEGNPSRYVMA
TDVILHLLSLLKDYDMNYRAVEFFNVPFSLDERLTLTNFVVEANAKTGIIGEEYTGDGYVKELEIELNSLNPLVAKPHNP
ANVVPVEEVEGTKIDQVFIGSCTNGRFEQISKAAEILEGEKVAVRTIVGPASMNVYKRMIEEGVARKLIEAGAVILPPGC
GPCLGRHMGVVGDGEIVLSTTNRNFRGRMGSPNAQIYLSNPITAAVSALYGEITNPEGAI

Sequences:

>Translated_380_residues
MTLIEEILGGKSGESVVRRVDLVYAHDGTMPLIIEAFNKVFATVRARAYIFFDHVYPAPTVKIANLQKEIRDFAKRHRIP
VIEGQGISHQLVVEMGLTENSKIVVGADSHTPTLGALGVFAVGMGATDVAVILGLGKTWFRIPESVGVILEGNPSRYVMA
TDVILHLLSLLKDYDMNYRAVEFFNVPFSLDERLTLTNFVVEANAKTGIIGEEYTGDGYVKELEIELNSLNPLVAKPHNP
ANVVPVEEVEGTKIDQVFIGSCTNGRFEQISKAAEILEGEKVAVRTIVGPASMNVYKRMIEEGVARKLIEAGAVILPPGC
GPCLGRHMGVVGDGEIVLSTTNRNFRGRMGSPNAQIYLSNPITAAVSALYGEITNPEGAI
>Mature_379_residues
TLIEEILGGKSGESVVRRVDLVYAHDGTMPLIIEAFNKVFATVRARAYIFFDHVYPAPTVKIANLQKEIRDFAKRHRIPV
IEGQGISHQLVVEMGLTENSKIVVGADSHTPTLGALGVFAVGMGATDVAVILGLGKTWFRIPESVGVILEGNPSRYVMAT
DVILHLLSLLKDYDMNYRAVEFFNVPFSLDERLTLTNFVVEANAKTGIIGEEYTGDGYVKELEIELNSLNPLVAKPHNPA
NVVPVEEVEGTKIDQVFIGSCTNGRFEQISKAAEILEGEKVAVRTIVGPASMNVYKRMIEEGVARKLIEAGAVILPPGCG
PCLGRHMGVVGDGEIVLSTTNRNFRGRMGSPNAQIYLSNPITAAVSALYGEITNPEGAI

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=450, Percent_Identity=26.2222222222222, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1786259, Length=358, Percent_Identity=28.7709497206704, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI87081781, Length=333, Percent_Identity=27.3273273273273, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI2367097, Length=158, Percent_Identity=36.7088607594937, Blast_Score=82, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=452, Percent_Identity=25.2212389380531, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI32564738, Length=346, Percent_Identity=26.3005780346821, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25149342, Length=288, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320440, Length=421, Percent_Identity=29.4536817102138, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6323335, Length=450, Percent_Identity=27.7777777777778, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321429, Length=424, Percent_Identity=25, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322261, Length=361, Percent_Identity=26.8698060941828, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28571643, Length=430, Percent_Identity=27.4418604651163, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC2_PYRFU (Q8U0C0)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579408.1
- ProteinModelPortal:   Q8U0C0
- SMR:   Q8U0C0
- EnsemblBacteria:   EBPYRT00000005256
- GeneID:   1469556
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1679
- NMPDR:   fig|186497.1.peg.1727
- GeneTree:   EBGT00050000022452
- HOGENOM:   HBG330745
- OMA:   EGTKIDQ
- ProtClustDB:   PRK00402
- BRENDA:   4.2.1.33
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 41175; Mature: 41044

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIEEILGGKSGESVVRRVDLVYAHDGTMPLIIEAFNKVFATVRARAYIFFDHVYPAPT
CCHHHHHHCCCCCHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCC
VKIANLQKEIRDFAKRHRIPVIEGQGISHQLVVEMGLTENSKIVVGADSHTPTLGALGVF
EEHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCEEEEECCCCCCCHHHHHHH
AVGMGATDVAVILGLGKTWFRIPESVGVILEGNPSRYVMATDVILHLLSLLKDYDMNYRA
HHCCCCHHHHHHHHCCHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCEEE
VEFFNVPFSLDERLTLTNFVVEANAKTGIIGEEYTGDGYVKELEIELNSLNPLVAKPHNP
EEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEECCCCC
ANVVPVEEVEGTKIDQVFIGSCTNGRFEQISKAAEILEGEKVAVRTIVGPASMNVYKRMI
CCEEEHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHH
EEGVARKLIEAGAVILPPGCGPCLGRHMGVVGDGEIVLSTTNRNFRGRMGSPNAQIYLSN
HHHHHHHHHHCCCEEECCCCCHHHHCCCCEEECCCEEEEECCCCCCCCCCCCCCEEEECC
PITAAVSALYGEITNPEGAI
CHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TLIEEILGGKSGESVVRRVDLVYAHDGTMPLIIEAFNKVFATVRARAYIFFDHVYPAPT
CHHHHHHCCCCCHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCC
VKIANLQKEIRDFAKRHRIPVIEGQGISHQLVVEMGLTENSKIVVGADSHTPTLGALGVF
EEHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCEEEEECCCCCCCHHHHHHH
AVGMGATDVAVILGLGKTWFRIPESVGVILEGNPSRYVMATDVILHLLSLLKDYDMNYRA
HHCCCCHHHHHHHHCCHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCEEE
VEFFNVPFSLDERLTLTNFVVEANAKTGIIGEEYTGDGYVKELEIELNSLNPLVAKPHNP
EEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEECCCCC
ANVVPVEEVEGTKIDQVFIGSCTNGRFEQISKAAEILEGEKVAVRTIVGPASMNVYKRMI
CCEEEHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHH
EEGVARKLIEAGAVILPPGCGPCLGRHMGVVGDGEIVLSTTNRNFRGRMGSPNAQIYLSN
HHHHHHHHHHCCCEEECCCCCHHHHCCCCEEECCCEEEEECCCCCCCCCCCCCCEEEECC
PITAAVSALYGEITNPEGAI
CHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA