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The map label for this gene is birA [H]

Identifier: 18978048

GI number: 18978048

Start: 1559206

End: 1559943

Strand: Reverse

Name: birA [H]

Synonym: PF1676

Alternate gene names: 18978048

Gene position: 1559943-1559206 (Counterclockwise)

Preceding gene: 18978077

Following gene: 18978046

Centisome position: 81.75

GC content: 34.96

Gene sequence:

>738_bases
ATGTTGAAGGATAGTGCACCAAAAAGGAAAATACTTGAGGAGTTGAGAAAAGGAGAAACGGTTTCGGGAGACTATTTAGC
CTCAAAATTAGGAGTATCTAGAGTTGCCATTTGGAAACACATTAGGGAATTAAAGGAGCTAGGGTATGGAATAATCGCCG
ATAAAAAGGGATATAAGCTAGTTTATGAGCCGAAAAAACCTTATCCCTGGGAAATTGACGTCCAATCATATTATCTCAAA
AAGACGACCTCTACGATGGAAGTAGCTAAAAAATTAGCCGAAAAAGGTGAAAAAAGTTTTACAGTGATTATAGCTGAAGA
ACAGACCCAGGGAAGAGGAAAGTTGAAGAAAAAATGGGAGTCAAAACCAGGAGGATTGTATTTTTCTATAATATTAAGGC
CAAAAATTAAACTAGTAGATGTTAAGAATTTGGCTCCCATACTTTCTTCTGCAATCCTTAATACTTTGAAAAAATTAGGA
ATTGAAGGAAATGCAAACGATAAAGGTGAAATTTTTGTAAATGGGAAAAAGATAGGAGGAATACTAATAGAAGCCAAAGG
AGAGCTTGACATAGTTGAGTATGTTATTATAGGTGTTGGGATAAACGTTAATAATGATGTTACTTACCCATTAGCAACAT
CACTGAAAAAAGAACTTGGGAGGGAAGTAGATTTACTAAAGTTCTCTAAGGACCTCTTATCAAACATGTTAAATGCTTTG
AGAGGCAACTTCAATTAA

Upstream 100 bases:

>100_bases
CTCATATGACCACCCACGTTACAATTTCGTTAACCTTTAAATATATTTTGGTTAACCACAAGGATGAAATTTTTGAAGCA
TTTTGGGAATATCATTGAAG

Downstream 100 bases:

>100_bases
TTTTTTGATATGGATTTCCGCTGAATCAAAGACTTCTATACTCACGTCACCCCGCTTGACAGCCAGAGGTAGTTCAGTAC
ATCCAAGAATTACTCCCTCA

Product: biotin operon repressor/biotin--[acetyl CoA carboxylase] ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MLKDSAPKRKILEELRKGETVSGDYLASKLGVSRVAIWKHIRELKELGYGIIADKKGYKLVYEPKKPYPWEIDVQSYYLK
KTTSTMEVAKKLAEKGEKSFTVIIAEEQTQGRGKLKKKWESKPGGLYFSIILRPKIKLVDVKNLAPILSSAILNTLKKLG
IEGNANDKGEIFVNGKKIGGILIEAKGELDIVEYVIIGVGINVNNDVTYPLATSLKKELGREVDLLKFSKDLLSNMLNAL
RGNFN

Sequences:

>Translated_245_residues
MLKDSAPKRKILEELRKGETVSGDYLASKLGVSRVAIWKHIRELKELGYGIIADKKGYKLVYEPKKPYPWEIDVQSYYLK
KTTSTMEVAKKLAEKGEKSFTVIIAEEQTQGRGKLKKKWESKPGGLYFSIILRPKIKLVDVKNLAPILSSAILNTLKKLG
IEGNANDKGEIFVNGKKIGGILIEAKGELDIVEYVIIGVGINVNNDVTYPLATSLKKELGREVDLLKFSKDLLSNMLNAL
RGNFN
>Mature_245_residues
MLKDSAPKRKILEELRKGETVSGDYLASKLGVSRVAIWKHIRELKELGYGIIADKKGYKLVYEPKKPYPWEIDVQSYYLK
KTTSTMEVAKKLAEKGEKSFTVIIAEEQTQGRGKLKKKWESKPGGLYFSIILRPKIKLVDVKNLAPILSSAILNTLKKLG
IEGNANDKGEIFVNGKKIGGILIEAKGELDIVEYVIIGVGINVNNDVTYPLATSLKKELGREVDLLKFSKDLLSNMLNAL
RGNFN

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=215, Percent_Identity=29.7674418604651, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 27382; Mature: 27382

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKDSAPKRKILEELRKGETVSGDYLASKLGVSRVAIWKHIRELKELGYGIIADKKGYKL
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEECCCCCEE
VYEPKKPYPWEIDVQSYYLKKTTSTMEVAKKLAEKGEKSFTVIIAEEQTQGRGKLKKKWE
EECCCCCCCEEECHHHEEEHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHC
SKPGGLYFSIILRPKIKLVDVKNLAPILSSAILNTLKKLGIEGNANDKGEIFVNGKKIGG
CCCCCEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECEEECE
ILIEAKGELDIVEYVIIGVGINVNNDVTYPLATSLKKELGREVDLLKFSKDLLSNMLNAL
EEEEECCCHHEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RGNFN
CCCCC
>Mature Secondary Structure
MLKDSAPKRKILEELRKGETVSGDYLASKLGVSRVAIWKHIRELKELGYGIIADKKGYKL
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEECCCCCEE
VYEPKKPYPWEIDVQSYYLKKTTSTMEVAKKLAEKGEKSFTVIIAEEQTQGRGKLKKKWE
EECCCCCCCEEECHHHEEEHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHC
SKPGGLYFSIILRPKIKLVDVKNLAPILSSAILNTLKKLGIEGNANDKGEIFVNGKKIGG
CCCCCEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECEEECE
ILIEAKGELDIVEYVIIGVGINVNNDVTYPLATSLKKELGREVDLLKFSKDLLSNMLNAL
EEEEECCCHHEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RGNFN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]