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The map label for this gene is hisA

Identifier: 18978034

GI number: 18978034

Start: 1546795

End: 1547517

Strand: Direct

Name: hisA

Synonym: PF1662

Alternate gene names: 18978034

Gene position: 1546795-1547517 (Clockwise)

Preceding gene: 18978033

Following gene: 18978035

Centisome position: 81.06

GC content: 39.83

Gene sequence:

>723_bases
ATGAAGTTTAGAATTTACCCAGCCATTGATATAATGGATGGAAAAGTAGTAAGGTTGTATAAAGGAAAGAAGGACGAAGT
AAAGGTGTATGGAGATCCACTGAAGTTTGCAGAGAAATTTTCAGAATACGTGGATAAAATCCACATAGTGGATCTGGATG
GGGCATTCTCCGGCAAACCCCAAAATCTTGACATTGTAGAGAAGATAATTAGAGATGTTGGAGTAAGAGTGCAGATAGGG
GGAGGATTTAGAGATTATGAGTCGATCAAGAAGGCGTACGACATAGGAGTTGAGAATGTAATTATAGGAACAAAAGCCCT
TGATATCTCTTTTTTGGAAAGAATAACCTCAGAATTCGAGGGAATAACAGTCAGCTTGGACTCCAAAGGGGGAAAGATTT
TCACCAAAGGTTGGATAGAGGAAGAGAGCCTTCTTGTAGAGGATGCATACAAAATGCTCCGAAAATTCGTTAATAGGTTT
GTATACACAGTCATAGATAGAGACGGTACCCTCCTGGGAATAGAGAAAATCAAAAGGTTCTGGGGAGATGAAGAATTTAT
TTATGCAGGAGGAGTGGCAACACCTACCGATATAATTCAGCTGGCTGAAATAGGGTTTTCTGGCGTTATTATTGGGAAGG
CTTTGTATGAAGGAACTATCTCTCTTGAAGAAGCTTTGGAGGTTGCTAAAAGTGTTGGCAAGAAGAATTATAGCAGCTCT
TGA

Upstream 100 bases:

>100_bases
TCCTCAGTATGCTTTGAAAACATATTTGGCGTCCAGTTCCACCCAGAAAAGAGTAGCAAAAATGGTCTTATACTCCTAAG
AAACTTTAGGAGGTTATAAA

Downstream 100 bases:

>100_bases
CATAAAAGATGGTAGGGTTGTTAAAGGAATAAAATTCAAGAACATTAGAGATGCTGGAGATCCAATAGAACTAGCTAAAA
GGTATGAGAGCGAAGGAATT

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKFRIYPAIDIMDGKVVRLYKGKKDEVKVYGDPLKFAEKFSEYVDKIHIVDLDGAFSGKPQNLDIVEKIIRDVGVRVQIG
GGFRDYESIKKAYDIGVENVIIGTKALDISFLERITSEFEGITVSLDSKGGKIFTKGWIEEESLLVEDAYKMLRKFVNRF
VYTVIDRDGTLLGIEKIKRFWGDEEFIYAGGVATPTDIIQLAEIGFSGVIIGKALYEGTISLEEALEVAKSVGKKNYSSS

Sequences:

>Translated_240_residues
MKFRIYPAIDIMDGKVVRLYKGKKDEVKVYGDPLKFAEKFSEYVDKIHIVDLDGAFSGKPQNLDIVEKIIRDVGVRVQIG
GGFRDYESIKKAYDIGVENVIIGTKALDISFLERITSEFEGITVSLDSKGGKIFTKGWIEEESLLVEDAYKMLRKFVNRF
VYTVIDRDGTLLGIEKIKRFWGDEEFIYAGGVATPTDIIQLAEIGFSGVIIGKALYEGTISLEEALEVAKSVGKKNYSSS
>Mature_240_residues
MKFRIYPAIDIMDGKVVRLYKGKKDEVKVYGDPLKFAEKFSEYVDKIHIVDLDGAFSGKPQNLDIVEKIIRDVGVRVQIG
GGFRDYESIKKAYDIGVENVIIGTKALDISFLERITSEFEGITVSLDSKGGKIFTKGWIEEESLLVEDAYKMLRKFVNRF
VYTVIDRDGTLLGIEKIKRFWGDEEFIYAGGVATPTDIIQLAEIGFSGVIIGKALYEGTISLEEALEVAKSVGKKNYSSS

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=29.6296296296296, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_PYRFU (P58791)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579391.1
- ProteinModelPortal:   P58791
- SMR:   P58791
- EnsemblBacteria:   EBPYRT00000005660
- GeneID:   1469539
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1662
- NMPDR:   fig|186497.1.peg.1710
- GeneTree:   EBGT00050000023418
- HOGENOM:   HBG541613
- OMA:   LLAINGW
- ProtClustDB:   PRK04128
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26877; Mature: 26877

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 127-127

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFRIYPAIDIMDGKVVRLYKGKKDEVKVYGDPLKFAEKFSEYVDKIHIVDLDGAFSGKP
CEEEEEEEEEECCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCEEEEEEECCCCCCCC
QNLDIVEKIIRDVGVRVQIGGGFRDYESIKKAYDIGVENVIIGTKALDISFLERITSEFE
CCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHC
GITVSLDSKGGKIFTKGWIEEESLLVEDAYKMLRKFVNRFVYTVIDRDGTLLGIEKIKRF
CEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEECHHHHHHH
WGDEEFIYAGGVATPTDIIQLAEIGFSGVIIGKALYEGTISLEEALEVAKSVGKKNYSSS
HCCCCEEEECCCCCHHHHHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKFRIYPAIDIMDGKVVRLYKGKKDEVKVYGDPLKFAEKFSEYVDKIHIVDLDGAFSGKP
CEEEEEEEEEECCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCEEEEEEECCCCCCCC
QNLDIVEKIIRDVGVRVQIGGGFRDYESIKKAYDIGVENVIIGTKALDISFLERITSEFE
CCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHC
GITVSLDSKGGKIFTKGWIEEESLLVEDAYKMLRKFVNRFVYTVIDRDGTLLGIEKIKRF
CEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEECHHHHHHH
WGDEEFIYAGGVATPTDIIQLAEIGFSGVIIGKALYEGTISLEEALEVAKSVGKKNYSSS
HCCCCEEEECCCCCHHHHHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA