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The map label for this gene is hisH

Identifier: 18978033

GI number: 18978033

Start: 1546209

End: 1546793

Strand: Direct

Name: hisH

Synonym: PF1661

Alternate gene names: 18978033

Gene position: 1546209-1546793 (Clockwise)

Preceding gene: 18978032

Following gene: 18978034

Centisome position: 81.03

GC content: 41.03

Gene sequence:

>585_bases
ATGGATAGAATTGCAATAGTCGACCTAGGAATTGGTAATCTAGCAAACGTAAAGAAGGCCCTAAAAGGATATATAACTAG
CGATCCTTACGAGATAGAGAAAGCAGACAAAATTGTTCTTCCCGGGGTTGGCAATTTCGGGGCCGTGGTGGACAAACTCG
CCCCAATAAAAGATATAATCATTGAGGGAATAAACGAGGGAAAGCCCTTCCTGGGAATATGTCTTGGAATGCAACTTCTT
TTCGAGGAAAGTGAAGAAAGCCCAGGAAAAGAGGGACTTGGAATATTCAAAGGAAAAGTTGTGAAACTAAAAAATGTAAG
AACCCCACATATTGGCTGGAACCAGGTGTGGATAAAGAAGGAATGCAAGCTCTTTGAGGGGCTAAAGAATGGCAGCTATT
TTTACTTTGTTCACTCCTATCATGCCGTCCCACAAGACCCCGATATAATAGCAACAACTACAGACTATGAAAATGCAGAG
TTTGTCTCCTCAGTATGCTTTGAAAACATATTTGGCGTCCAGTTCCACCCAGAAAAGAGTAGCAAAAATGGTCTTATACT
CCTAAGAAACTTTAGGAGGTTATAA

Upstream 100 bases:

>100_bases
CCCACCATATAGTAGAGGCCGCATTTAAGGGGCTTGGAATGGCTCTGAGAGTAGCAACTAAAGAGAGTGAGAGAGTTGAA
AGCACAAAGGGGGTATTGTA

Downstream 100 bases:

>100_bases
AATGAAGTTTAGAATTTACCCAGCCATTGATATAATGGATGGAAAAGTAGTAAGGTTGTATAAAGGAAAGAAGGACGAAG
TAAAGGTGTATGGAGATCCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MDRIAIVDLGIGNLANVKKALKGYITSDPYEIEKADKIVLPGVGNFGAVVDKLAPIKDIIIEGINEGKPFLGICLGMQLL
FEESEESPGKEGLGIFKGKVVKLKNVRTPHIGWNQVWIKKECKLFEGLKNGSYFYFVHSYHAVPQDPDIIATTTDYENAE
FVSSVCFENIFGVQFHPEKSSKNGLILLRNFRRL

Sequences:

>Translated_194_residues
MDRIAIVDLGIGNLANVKKALKGYITSDPYEIEKADKIVLPGVGNFGAVVDKLAPIKDIIIEGINEGKPFLGICLGMQLL
FEESEESPGKEGLGIFKGKVVKLKNVRTPHIGWNQVWIKKECKLFEGLKNGSYFYFVHSYHAVPQDPDIIATTTDYENAE
FVSSVCFENIFGVQFHPEKSSKNGLILLRNFRRL
>Mature_194_residues
MDRIAIVDLGIGNLANVKKALKGYITSDPYEIEKADKIVLPGVGNFGAVVDKLAPIKDIIIEGINEGKPFLGICLGMQLL
FEESEESPGKEGLGIFKGKVVKLKNVRTPHIGWNQVWIKKECKLFEGLKNGSYFYFVHSYHAVPQDPDIIATTTDYENAE
FVSSVCFENIFGVQFHPEKSSKNGLILLRNFRRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=42.5, Blast_Score=138, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319725, Length=210, Percent_Identity=36.6666666666667, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_PYRFU (P58789)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579390.1
- ProteinModelPortal:   P58789
- SMR:   P58789
- EnsemblBacteria:   EBPYRT00000004755
- GeneID:   1469538
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1661
- NMPDR:   fig|186497.1.peg.1709
- GeneTree:   EBGT00050000023479
- HOGENOM:   HBG292341
- OMA:   VCVLKEV
- ProtClustDB:   PRK13143
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 21660; Mature: 21660

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 74-74 ACT_SITE 176-176 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRIAIVDLGIGNLANVKKALKGYITSDPYEIEKADKIVLPGVGNFGAVVDKLAPIKDII
CCCEEEEEECCCCHHHHHHHHHCCCCCCCEEECCCCEEEECCCCCHHHHHHHHCCHHHHH
IEGINEGKPFLGICLGMQLLFEESEESPGKEGLGIFKGKVVKLKNVRTPHIGWNQVWIKK
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEECCCEEEEEECCCCCCCCCCEEEHHH
ECKLFEGLKNGSYFYFVHSYHAVPQDPDIIATTTDYENAEFVSSVCFENIFGVQFHPEKS
HHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEECCCCC
SKNGLILLRNFRRL
CCCCEEEEECCCCC
>Mature Secondary Structure
MDRIAIVDLGIGNLANVKKALKGYITSDPYEIEKADKIVLPGVGNFGAVVDKLAPIKDII
CCCEEEEEECCCCHHHHHHHHHCCCCCCCEEECCCCEEEECCCCCHHHHHHHHCCHHHHH
IEGINEGKPFLGICLGMQLLFEESEESPGKEGLGIFKGKVVKLKNVRTPHIGWNQVWIKK
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEECCCEEEEEECCCCCCCCCCEEEHHH
ECKLFEGLKNGSYFYFVHSYHAVPQDPDIIATTTDYENAEFVSSVCFENIFGVQFHPEKS
HHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEECCCCC
SKNGLILLRNFRRL
CCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA