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The map label for this gene is rpoA2 [H]

Identifier: 18977934

GI number: 18977934

Start: 1455699

End: 1456892

Strand: Reverse

Name: rpoA2 [H]

Synonym: PF1562

Alternate gene names: 18977934

Gene position: 1456892-1455699 (Counterclockwise)

Preceding gene: 18977935

Following gene: 18977933

Centisome position: 76.35

GC content: 40.7

Gene sequence:

>1194_bases
ATGGTCTCTCTTTCTACTATTAAATCTTTAATTGAAAAGAAGGGAGCAAATCTTCCTGAAAATGTGAAATCTGAACTTTA
TGAGAAGCTTAAGAAGTATAACGAGAAGTACAAATTAACGAAGGCTGAAATTGAGGCAATCATTGATGATGTTGTAAAAG
AATATGAAAGGGCATTAGTTGAACCTGGGGAGCCAGTTGGTACTGTTGCTGCCCAGTCTATTGGAGAACCTTCAACCCAG
ATGACTCTCAACACTTTCCACTATGCAGGTGTTGCTGAAATTAACGTTACTTTAGGTTTGCCAAGAATTATAGAAATCGT
GGATGCTAGGAAAAATCCATCAACCCCCATGATGACTGTATACTTAGATGAGGAACACAGATATGACAGGGCAAAGGCCG
AGGAAGTGGCTAGAAGAATTGAAGGAACAACTTTAGAAAATCTTGCCAGATCAACAACTTTGGACTTAATAAACTTTGAA
TTTATAGTTGAAATTGATCCTGAGAGGCTCGAGAGAAGTGGACTTACTATGGAAAAAGTAGTCAAAAAGCTAGAAAGTTC
ATTCAAGAGCGCTGAGTTTGAGGTTGATGGGTATACCCTAATTGTAAGGCCAAAGAAGGCAGATAAGATTTCTGACCTCA
GAAGATTTGCCGAAAAGATTAAAAAGCACCGTCTTAAGGGTCTCTCGGGCGTCGGTAAAACAATAGTGAGGAAAGAGGGA
GACGAGTACGTAATATACACTGAAGGCTCCAACTTTAAGCAAGTCTTAAAAGTCCCGGGTGTAGATCCGACAAGAACTAG
GACAAATAACATACACGAAATTGCAGAGGTGCTTGGAATAGAGGCCGCAAGAAATGCAATAATTGATGAAATAGTGAGTA
CAATGCAGGAACAAGGTCTAGAAGTAGATATAAGGCACATCATGCTCGTTGCAGACATGATGACTTTAGATGGGATAGTG
AGGCCAATTGGTAGACATGGAGTTGTTGGAGAGAAGAGTAGTGTACTTGCAAGAGCTGCCTTCGAGATTACTGTCCAGCA
CTTATTTGAGGCTGCTGAAAAAGGTGAAGTTGACAACTTAAACGGTGTAATTGAAAACGTCTTAATCGGGCAGCCCGTTC
CAGTGGGAACGGGCATGGTTAAGTTAACTATGAAACTACCACTAAGACCACAGAAAGAAAAGGAGGAGGTGTGA

Upstream 100 bases:

>100_bases
AAGAGTTGGAGAGGCAAGACAGTTGATGTAGATAGAATTATTGTGAGGACTCTCTTAAAGATGAGAGGAAATAACAAGGC
GAAGGTGTGAGGTGGTTTCT

Downstream 100 bases:

>100_bases
TAGATGGATTTAGCATTTGAGCTTAGAAAAGCTATGGAAACTGGAAAAGTTGTCCTCGGTTCTAATGAAACAATTAGGTT
AGCAAAGACTGGAGGAGCAA

Product: DNA-directed RNA polymerase subunit A''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MVSLSTIKSLIEKKGANLPENVKSELYEKLKKYNEKYKLTKAEIEAIIDDVVKEYERALVEPGEPVGTVAAQSIGEPSTQ
MTLNTFHYAGVAEINVTLGLPRIIEIVDARKNPSTPMMTVYLDEEHRYDRAKAEEVARRIEGTTLENLARSTTLDLINFE
FIVEIDPERLERSGLTMEKVVKKLESSFKSAEFEVDGYTLIVRPKKADKISDLRRFAEKIKKHRLKGLSGVGKTIVRKEG
DEYVIYTEGSNFKQVLKVPGVDPTRTRTNNIHEIAEVLGIEAARNAIIDEIVSTMQEQGLEVDIRHIMLVADMMTLDGIV
RPIGRHGVVGEKSSVLARAAFEITVQHLFEAAEKGEVDNLNGVIENVLIGQPVPVGTGMVKLTMKLPLRPQKEKEEV

Sequences:

>Translated_397_residues
MVSLSTIKSLIEKKGANLPENVKSELYEKLKKYNEKYKLTKAEIEAIIDDVVKEYERALVEPGEPVGTVAAQSIGEPSTQ
MTLNTFHYAGVAEINVTLGLPRIIEIVDARKNPSTPMMTVYLDEEHRYDRAKAEEVARRIEGTTLENLARSTTLDLINFE
FIVEIDPERLERSGLTMEKVVKKLESSFKSAEFEVDGYTLIVRPKKADKISDLRRFAEKIKKHRLKGLSGVGKTIVRKEG
DEYVIYTEGSNFKQVLKVPGVDPTRTRTNNIHEIAEVLGIEAARNAIIDEIVSTMQEQGLEVDIRHIMLVADMMTLDGIV
RPIGRHGVVGEKSSVLARAAFEITVQHLFEAAEKGEVDNLNGVIENVLIGQPVPVGTGMVKLTMKLPLRPQKEKEEV
>Mature_397_residues
MVSLSTIKSLIEKKGANLPENVKSELYEKLKKYNEKYKLTKAEIEAIIDDVVKEYERALVEPGEPVGTVAAQSIGEPSTQ
MTLNTFHYAGVAEINVTLGLPRIIEIVDARKNPSTPMMTVYLDEEHRYDRAKAEEVARRIEGTTLENLARSTTLDLINFE
FIVEIDPERLERSGLTMEKVVKKLESSFKSAEFEVDGYTLIVRPKKADKISDLRRFAEKIKKHRLKGLSGVGKTIVRKEG
DEYVIYTEGSNFKQVLKVPGVDPTRTRTNNIHEIAEVLGIEAARNAIIDEIVSTMQEQGLEVDIRHIMLVADMMTLDGIV
RPIGRHGVVGEKSSVLARAAFEITVQHLFEAAEKGEVDNLNGVIENVLIGQPVPVGTGMVKLTMKLPLRPQKEKEEV

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family [H]

Homologues:

Organism=Homo sapiens, GI39725938, Length=399, Percent_Identity=37.593984962406, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI4505939, Length=430, Percent_Identity=30.9302325581395, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI103471997, Length=102, Percent_Identity=49.0196078431373, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25145495, Length=396, Percent_Identity=39.3939393939394, Blast_Score=245, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI71987878, Length=419, Percent_Identity=32.2195704057279, Blast_Score=191, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI71998295, Length=100, Percent_Identity=47, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320061, Length=407, Percent_Identity=34.1523341523341, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6324690, Length=394, Percent_Identity=35.0253807106599, Blast_Score=195, Evalue=9e-51,
Organism=Saccharomyces cerevisiae, GI6324917, Length=107, Percent_Identity=44.8598130841121, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281360912, Length=362, Percent_Identity=38.3977900552486, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI17530899, Length=424, Percent_Identity=32.0754716981132, Blast_Score=205, Evalue=4e-53,
Organism=Drosophila melanogaster, GI17647875, Length=173, Percent_Identity=39.3063583815029, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007081
- InterPro:   IPR012757 [H]

Pfam domain/function: PF04998 RNA_pol_Rpb1_5 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 44405; Mature: 44405

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLSTIKSLIEKKGANLPENVKSELYEKLKKYNEKYKLTKAEIEAIIDDVVKEYERALV
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPGEPVGTVAAQSIGEPSTQMTLNTFHYAGVAEINVTLGLPRIIEIVDARKNPSTPMMTV
CCCCCHHHHHHHHCCCCCHHEEEEHEEECCEEEEEEEECCHHHHHHHHCCCCCCCCEEEE
YLDEEHRYDRAKAEEVARRIEGTTLENLARSTTLDLINFEFIVEIDPERLERSGLTMEKV
EECCHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEEEEEECHHHHHHCCCCHHHH
VKKLESSFKSAEFEVDGYTLIVRPKKADKISDLRRFAEKIKKHRLKGLSGVGKTIVRKEG
HHHHHHHHHCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
DEYVIYTEGSNFKQVLKVPGVDPTRTRTNNIHEIAEVLGIEAARNAIIDEIVSTMQEQGL
CCEEEEECCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVDIRHIMLVADMMTLDGIVRPIGRHGVVGEKSSVLARAAFEITVQHLFEAAEKGEVDNL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
NGVIENVLIGQPVPVGTGMVKLTMKLPLRPQKEKEEV
HHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHCCCC
>Mature Secondary Structure
MVSLSTIKSLIEKKGANLPENVKSELYEKLKKYNEKYKLTKAEIEAIIDDVVKEYERALV
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPGEPVGTVAAQSIGEPSTQMTLNTFHYAGVAEINVTLGLPRIIEIVDARKNPSTPMMTV
CCCCCHHHHHHHHCCCCCHHEEEEHEEECCEEEEEEEECCHHHHHHHHCCCCCCCCEEEE
YLDEEHRYDRAKAEEVARRIEGTTLENLARSTTLDLINFEFIVEIDPERLERSGLTMEKV
EECCHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEEEEEECHHHHHHCCCCHHHH
VKKLESSFKSAEFEVDGYTLIVRPKKADKISDLRRFAEKIKKHRLKGLSGVGKTIVRKEG
HHHHHHHHHCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
DEYVIYTEGSNFKQVLKVPGVDPTRTRTNNIHEIAEVLGIEAARNAIIDEIVSTMQEQGL
CCEEEEECCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVDIRHIMLVADMMTLDGIVRPIGRHGVVGEKSSVLARAAFEITVQHLFEAAEKGEVDNL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
NGVIENVLIGQPVPVGTGMVKLTMKLPLRPQKEKEEV
HHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]