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The map label for this gene is hycE [C]

Identifier: 18977805

GI number: 18977805

Start: 1342249

End: 1342770

Strand: Direct

Name: hycE [C]

Synonym: PF1433

Alternate gene names: 18977805

Gene position: 1342249-1342770 (Clockwise)

Preceding gene: 18977804

Following gene: 18977806

Centisome position: 70.34

GC content: 42.53

Gene sequence:

>522_bases
ATGAGTAAAGCTGAAATGGTTGCAAATAAGATAAAGGAGAGGTTTCCAAACGCTGAAGTTGTAGTAAAAACCAACAAGTG
GGGAAGAGAAAGAGTCTGGGTTAGAATATCTAGAGAGGAATACAAAGAGCTAATGAAATTTATAAGGGAACTTGACCCCG
AAGCCCACTATTCAATTGGAATCGAGCAAGATTGGGGAGATGAGCTTGGATTTCTGAATCATATCCTCTTATTCTACGAC
GAGCCCCCTGGGGTATCTCTGCTAATTGATGTCCATGCTCCAAAAGATAATCCCGTACTACCAGACACTAGCGATATATT
CCCAATATCATTGCAATTTGAAAGGGAAGGAATGGAAATGGTGGGGCTTGACTTTGAAGGAGCTCCAGATAAGAGAAGAC
TCTTCTTACCAGACGACTTTCCAGAGGGAATATACCCATTAAGAACTGATGAAAAAGGTGTTCCGGAGGAGATGGTCAAA
AACGCTGGCCACCCATACCTTCTCAGGAGGGAGAAGAAATGA

Upstream 100 bases:

>100_bases
CCACCTAGGCCAGAGGCAATTCTCCATGGAGTCGTATTAGCTCTAGAAAAGCTTGCCAAGATGATAAAAGGTGAAGTTCC
GCCTGAGGAAGGTGAGGAAA

Downstream 100 bases:

>100_bases
AGAAGGTCGAATATTGGGTTAAAATCCCCTTTGGTCCCATTCATCCAGGATTAGAAGAGCCAGAAAAGTTCATCATAACC
CTAGATGGAGAGAGAATAGT

Product: mbh11 membrane bound hydrogenase beta (NADH dehydrogenase)

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: Membrane Bound Hydrogenase MbhK Subunit; Membrane-Bound Hydrogenase MBH 2 Subunit Mbh2K; NAD(P)H-Quinone Oxidoreductase Subunit J; Mbh11 Membrane Bound Hydrogenase Beta; Hydrogenase Related Subunit; Membrane-Bound Hydrogenase MBH 1 Subunit Mbh1K; Membrane Bound Hydrogenase Beta; Energy-Conserving NiFe-Hydrogenase Subunit Beta

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MSKAEMVANKIKERFPNAEVVVKTNKWGRERVWVRISREEYKELMKFIRELDPEAHYSIGIEQDWGDELGFLNHILLFYD
EPPGVSLLIDVHAPKDNPVLPDTSDIFPISLQFEREGMEMVGLDFEGAPDKRRLFLPDDFPEGIYPLRTDEKGVPEEMVK
NAGHPYLLRREKK

Sequences:

>Translated_173_residues
MSKAEMVANKIKERFPNAEVVVKTNKWGRERVWVRISREEYKELMKFIRELDPEAHYSIGIEQDWGDELGFLNHILLFYD
EPPGVSLLIDVHAPKDNPVLPDTSDIFPISLQFEREGMEMVGLDFEGAPDKRRLFLPDDFPEGIYPLRTDEKGVPEEMVK
NAGHPYLLRREKK
>Mature_172_residues
SKAEMVANKIKERFPNAEVVVKTNKWGRERVWVRISREEYKELMKFIRELDPEAHYSIGIEQDWGDELGFLNHILLFYDE
PPGVSLLIDVHAPKDNPVLPDTSDIFPISLQFEREGMEMVGLDFEGAPDKRRLFLPDDFPEGIYPLRTDEKGVPEEMVKN
AGHPYLLRREKK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3262

COG function: function code C; Ni,Fe-hydrogenase III component G

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 20184; Mature: 20053

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAEMVANKIKERFPNAEVVVKTNKWGRERVWVRISREEYKELMKFIRELDPEAHYSIG
CCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECHHHHHHHHHHHHHCCCCCEEEEC
IEQDWGDELGFLNHILLFYDEPPGVSLLIDVHAPKDNPVLPDTSDIFPISLQFEREGMEM
CCCCCCCHHHHHEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEE
VGLDFEGAPDKRRLFLPDDFPEGIYPLRTDEKGVPEEMVKNAGHPYLLRREKK
EECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCC
>Mature Secondary Structure 
SKAEMVANKIKERFPNAEVVVKTNKWGRERVWVRISREEYKELMKFIRELDPEAHYSIG
CHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECHHHHHHHHHHHHHCCCCCEEEEC
IEQDWGDELGFLNHILLFYDEPPGVSLLIDVHAPKDNPVLPDTSDIFPISLQFEREGMEM
CCCCCCCHHHHHEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEE
VGLDFEGAPDKRRLFLPDDFPEGIYPLRTDEKGVPEEMVKNAGHPYLLRREKK
EECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA