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The map label for this gene is cooH [H]

Identifier: 18977806

GI number: 18977806

Start: 1342767

End: 1344050

Strand: Direct

Name: cooH [H]

Synonym: PF1434

Alternate gene names: 18977806

Gene position: 1342767-1344050 (Clockwise)

Preceding gene: 18977805

Following gene: 18977807

Centisome position: 70.37

GC content: 43.69

Gene sequence:

>1284_bases
ATGAAGAAGGTCGAATATTGGGTTAAAATCCCCTTTGGTCCCATTCATCCAGGATTAGAAGAGCCAGAAAAGTTCATCAT
AACCCTAGATGGAGAGAGAATAGTAAACGTTGATGTAAAGCTTGGATATAACCTCAGAGGTGTGCAATGGATAGGAATGA
GAAGAAACTACGTCCAGATCATGTATCTGGCAGAGAGAATGTGTGGAATTTGCTCCTTTTCCCATAACCACACATACGTA
AGAGCTGTTGAAGAGATGGCCGGAATAGAGGTTCCAGAGAGAGCAGAATATATCAGAGTTATAGTGGGGGAGCTCGAGAG
AATTCACTCCCACCTCTTGAATCTCGGAGTAGTGGGGCACGACATTGGATATGACACAGTCCTCCATCTAACATGGTTGG
CTAGAGAAAGAGTAATGGATGTCCTAGAAGCTGTTAGTGGAAACAGAGTTAACTACTCCATGGTAACCATTGGAGGAGTC
AGAAGGGATATAGGAGAGAAACAAAAACGACTAATTTTGGATATGATAAAATACTACAGAGAGGTTCTCCCACAAATAGA
AGACGTTTTTCTCCATGACTCAACAATTGAGGCAAGATTAAGGGATGTTGCAGTTGTTCCAAAAAAGCTTGCAATAGAGA
TGGGTGCCGTTGGCCCAACGGCGAGAGGAAGTGGGATTAAAGAGGATTCTAGATGGAGTGAGCAACTAGGAGTTTATCCC
GACCTGGGAATAAAACCAGTAACGCCCGAAGACGTTACTGGAGAAAAGGCCAGAGGAGATGTTTATGACAGAATGGCCGT
AAGAATTGGGGAGCTATGGATGAGTTTAGATTTGCTGGAACATGCACTCGATCAGATGCCGGAAGGAAAGATAAAAACAT
TCCCCAAAGACAATATCTTAGTCGCTAAGCTGAAGCTCCTTGGAGATGGAGAAGGAATAGGAAGGTACGAAGCTCCTAGA
GGAGAGCTAGTCCACTACGTGAGAGGACAAAAGGGAAGAGATGGGCCTGTAAGGTGGAAACCAAGAGAGCCAACCTTCCC
GAATCTATTCACAATCGCAAAGGCTCTAGAAGGAAATGAGCTTGCGGACTTAGTTGTTGCCATAGCTTCAATAGATCCAT
GTCTAAGCTGTACAGACAGAGTAGCCATAGTAAAAGAAGGAAAGAAGGTAGTTCTAACTGAAAAGGATCTATTAAAGCTT
TCTATTGAAAAGACAAAGGAGATCAACCCAAACGTAAAGGGAGATCCTACACCTACTGGAATTGGATGTTCTAGGGGGGT
GTGA

Upstream 100 bases:

>100_bases
TTCCAGAGGGAATATACCCATTAAGAACTGATGAAAAAGGTGTTCCGGAGGAGATGGTCAAAAACGCTGGCCACCCATAC
CTTCTCAGGAGGGAGAAGAA

Downstream 100 bases:

>100_bases
ATATGAAAATTGTATATGGAGTTATTGGCTTAATCCTCATTTACATTTACGTTTCCGTAGTTTCACTTTTATTTAGTGGT
ATAGATAGAAAGCTTGTAGC

Product: mbh12 membrane bound hydrogenase alpha (NADH dehydrogenase)

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKKVEYWVKIPFGPIHPGLEEPEKFIITLDGERIVNVDVKLGYNLRGVQWIGMRRNYVQIMYLAERMCGICSFSHNHTYV
RAVEEMAGIEVPERAEYIRVIVGELERIHSHLLNLGVVGHDIGYDTVLHLTWLARERVMDVLEAVSGNRVNYSMVTIGGV
RRDIGEKQKRLILDMIKYYREVLPQIEDVFLHDSTIEARLRDVAVVPKKLAIEMGAVGPTARGSGIKEDSRWSEQLGVYP
DLGIKPVTPEDVTGEKARGDVYDRMAVRIGELWMSLDLLEHALDQMPEGKIKTFPKDNILVAKLKLLGDGEGIGRYEAPR
GELVHYVRGQKGRDGPVRWKPREPTFPNLFTIAKALEGNELADLVVAIASIDPCLSCTDRVAIVKEGKKVVLTEKDLLKL
SIEKTKEINPNVKGDPTPTGIGCSRGV

Sequences:

>Translated_427_residues
MKKVEYWVKIPFGPIHPGLEEPEKFIITLDGERIVNVDVKLGYNLRGVQWIGMRRNYVQIMYLAERMCGICSFSHNHTYV
RAVEEMAGIEVPERAEYIRVIVGELERIHSHLLNLGVVGHDIGYDTVLHLTWLARERVMDVLEAVSGNRVNYSMVTIGGV
RRDIGEKQKRLILDMIKYYREVLPQIEDVFLHDSTIEARLRDVAVVPKKLAIEMGAVGPTARGSGIKEDSRWSEQLGVYP
DLGIKPVTPEDVTGEKARGDVYDRMAVRIGELWMSLDLLEHALDQMPEGKIKTFPKDNILVAKLKLLGDGEGIGRYEAPR
GELVHYVRGQKGRDGPVRWKPREPTFPNLFTIAKALEGNELADLVVAIASIDPCLSCTDRVAIVKEGKKVVLTEKDLLKL
SIEKTKEINPNVKGDPTPTGIGCSRGV
>Mature_427_residues
MKKVEYWVKIPFGPIHPGLEEPEKFIITLDGERIVNVDVKLGYNLRGVQWIGMRRNYVQIMYLAERMCGICSFSHNHTYV
RAVEEMAGIEVPERAEYIRVIVGELERIHSHLLNLGVVGHDIGYDTVLHLTWLARERVMDVLEAVSGNRVNYSMVTIGGV
RRDIGEKQKRLILDMIKYYREVLPQIEDVFLHDSTIEARLRDVAVVPKKLAIEMGAVGPTARGSGIKEDSRWSEQLGVYP
DLGIKPVTPEDVTGEKARGDVYDRMAVRIGELWMSLDLLEHALDQMPEGKIKTFPKDNILVAKLKLLGDGEGIGRYEAPR
GELVHYVRGQKGRDGPVRWKPREPTFPNLFTIAKALEGNELADLVVAIASIDPCLSCTDRVAIVKEGKKVVLTEKDLLKL
SIEKTKEINPNVKGDPTPTGIGCSRGV

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=393, Percent_Identity=28.2442748091603, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI260898743, Length=383, Percent_Identity=28.1984334203655, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1789076, Length=399, Percent_Identity=33.8345864661654, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1788832, Length=382, Percent_Identity=35.3403141361257, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI145693162, Length=395, Percent_Identity=29.3670886075949, Blast_Score=165, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17568379, Length=389, Percent_Identity=29.305912596401, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17555284, Length=389, Percent_Identity=28.7917737789203, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24638644, Length=393, Percent_Identity=29.0076335877863, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI221459469, Length=393, Percent_Identity=27.4809160305344, Blast_Score=151, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 47934; Mature: 47934

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVEYWVKIPFGPIHPGLEEPEKFIITLDGERIVNVDVKLGYNLRGVQWIGMRRNYVQI
CCCCEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCEEEECCCCCHHHH
MYLAERMCGICSFSHNHTYVRAVEEMAGIEVPERAEYIRVIVGELERIHSHLLNLGVVGH
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEC
DIGYDTVLHLTWLARERVMDVLEAVSGNRVNYSMVTIGGVRRDIGEKQKRLILDMIKYYR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHH
EVLPQIEDVFLHDSTIEARLRDVAVVPKKLAIEMGAVGPTARGSGIKEDSRWSEQLGVYP
HHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCC
DLGIKPVTPEDVTGEKARGDVYDRMAVRIGELWMSLDLLEHALDQMPEGKIKTFPKDNIL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEE
VAKLKLLGDGEGIGRYEAPRGELVHYVRGQKGRDGPVRWKPREPTFPNLFTIAKALEGNE
EEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCH
LADLVVAIASIDPCLSCTDRVAIVKEGKKVVLTEKDLLKLSIEKTKEINPNVKGDPTPTG
HHHHHHHHHHCCHHHCCCCCEEEEECCCEEEEECHHHHHEEHHHHHCCCCCCCCCCCCCC
IGCSRGV
CCCCCCC
>Mature Secondary Structure
MKKVEYWVKIPFGPIHPGLEEPEKFIITLDGERIVNVDVKLGYNLRGVQWIGMRRNYVQI
CCCCEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCEEEECCCCCHHHH
MYLAERMCGICSFSHNHTYVRAVEEMAGIEVPERAEYIRVIVGELERIHSHLLNLGVVGH
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEC
DIGYDTVLHLTWLARERVMDVLEAVSGNRVNYSMVTIGGVRRDIGEKQKRLILDMIKYYR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHH
EVLPQIEDVFLHDSTIEARLRDVAVVPKKLAIEMGAVGPTARGSGIKEDSRWSEQLGVYP
HHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCC
DLGIKPVTPEDVTGEKARGDVYDRMAVRIGELWMSLDLLEHALDQMPEGKIKTFPKDNIL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEE
VAKLKLLGDGEGIGRYEAPRGELVHYVRGQKGRDGPVRWKPREPTFPNLFTIAKALEGNE
EEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCH
LADLVVAIASIDPCLSCTDRVAIVKEGKKVVLTEKDLLKLSIEKTKEINPNVKGDPTPTG
HHHHHHHHHHCCHHHCCCCCEEEEECCCEEEEECHHHHHEEHHHHHCCCCCCCCCCCCCC
IGCSRGV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]