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The map label for this gene is pgaC [H]

Identifier: 18977731

GI number: 18977731

Start: 1277390

End: 1278295

Strand: Direct

Name: pgaC [H]

Synonym: PF1359

Alternate gene names: 18977731

Gene position: 1277390-1278295 (Clockwise)

Preceding gene: 18977730

Following gene: 18977732

Centisome position: 66.94

GC content: 36.2

Gene sequence:

>906_bases
ATGAAGGCGCATGTGTCAATTGTTGTTCCAACTTATAACCGCAAGAAAAAGTTACAGCAGTGTTTAAAGGCTCTTATAAA
TCAAAATTACCCCAAGGAAAGATATGAGATAGTTGTAGTAGATGATGGCTCTACAGATGGCACTTATGAGTTTCTTCAAG
AGACAAGAAAAGAAATTCAAAACCTAAGAGTATTAAGGCAACGAAATAAAGGCCCAGCAGCAGCTAGGAACCTGGGCATT
AAAAATGCCCAGGGAGAAATCGTATTTTTTGTTGATGATGATGTAATTGTTCCTAATAATTGGATTAAAGAGTTCTTGAA
TGTGTTTAGAAAATATCCCGAAGTTGCGGCTGTAAGTGGCTATGTAGAAGCTTCTGAAGAAGTTCTAAGAAAAAATATTT
TTGCACAGTATGAGGCTTATATGTCCAGACTTGCTTACAATATGCCAAGGACCATTTATATTGGAAGTTTTGAAACTTTT
GGAGGAGCAACTTGTAACGTTGCATATAAAAGGGAAGTTCTTGAGGAGGTGGGTGGATTTGATGAAACATTTCCAGTAGC
TGCTGGAGAAGATGCCGATTTAAAACTCAGAGTAGCTCTAAAAGGTTACAAATTTGCATTTATTCCACTAAAGGCTATTC
ACATCCAAGACTACACTCTTAAAGGATTTTGGAAACAGCAAGTTAATAGGGGGATAGGAAATTACTATTTTAGAAAAAAG
TGGCAGTCGTTTTTTAACAAAGACGAACTGAAAAACATTAGAGCGGCGCCTAACTACAACATCCCCCGTATGATATTAAA
AGATGGGAAACTATTAATGCTTGCTTTGTTTTTAATTGCAGTAATTGCAAACAGATATGGGCAAATAAAGGCAAGGAAGA
TTCTTAAAAAGGAGGAAGAAAAATGA

Upstream 100 bases:

>100_bases
AACATGTAGAAAAGTATACTTGGTATAATTATGGGGAGAATTTATATAATGAACTTAAAAGGCTTGGCCTGCTTTAAATT
AACAGTTATGGAGGGTTTAG

Downstream 100 bases:

>100_bases
GAAGTATTGTAGTTGTATACGGGTTGCCCGAGAAGTATGCAACTTCTATCCGACTAAAGAAATTACTGGGCGATACAAAA
GACAAAAATATCAGAACACG

Product: glycosyl transferase

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKAHVSIVVPTYNRKKKLQQCLKALINQNYPKERYEIVVVDDGSTDGTYEFLQETRKEIQNLRVLRQRNKGPAAARNLGI
KNAQGEIVFFVDDDVIVPNNWIKEFLNVFRKYPEVAAVSGYVEASEEVLRKNIFAQYEAYMSRLAYNMPRTIYIGSFETF
GGATCNVAYKREVLEEVGGFDETFPVAAGEDADLKLRVALKGYKFAFIPLKAIHIQDYTLKGFWKQQVNRGIGNYYFRKK
WQSFFNKDELKNIRAAPNYNIPRMILKDGKLLMLALFLIAVIANRYGQIKARKILKKEEEK

Sequences:

>Translated_301_residues
MKAHVSIVVPTYNRKKKLQQCLKALINQNYPKERYEIVVVDDGSTDGTYEFLQETRKEIQNLRVLRQRNKGPAAARNLGI
KNAQGEIVFFVDDDVIVPNNWIKEFLNVFRKYPEVAAVSGYVEASEEVLRKNIFAQYEAYMSRLAYNMPRTIYIGSFETF
GGATCNVAYKREVLEEVGGFDETFPVAAGEDADLKLRVALKGYKFAFIPLKAIHIQDYTLKGFWKQQVNRGIGNYYFRKK
WQSFFNKDELKNIRAAPNYNIPRMILKDGKLLMLALFLIAVIANRYGQIKARKILKKEEEK
>Mature_301_residues
MKAHVSIVVPTYNRKKKLQQCLKALINQNYPKERYEIVVVDDGSTDGTYEFLQETRKEIQNLRVLRQRNKGPAAARNLGI
KNAQGEIVFFVDDDVIVPNNWIKEFLNVFRKYPEVAAVSGYVEASEEVLRKNIFAQYEAYMSRLAYNMPRTIYIGSFETF
GGATCNVAYKREVLEEVGGFDETFPVAAGEDADLKLRVALKGYKFAFIPLKAIHIQDYTLKGFWKQQVNRGIGNYYFRKK
WQSFFNKDELKNIRAAPNYNIPRMILKDGKLLMLALFLIAVIANRYGQIKARKILKKEEEK

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=234, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790044, Length=96, Percent_Identity=36.4583333333333, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788372, Length=135, Percent_Identity=28.8888888888889, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 34823; Mature: 34823

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAHVSIVVPTYNRKKKLQQCLKALINQNYPKERYEIVVVDDGSTDGTYEFLQETRKEIQ
CCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
NLRVLRQRNKGPAAARNLGIKNAQGEIVFFVDDDVIVPNNWIKEFLNVFRKYPEVAAVSG
HHHHHHHCCCCCHHHHHCCCCCCCCCEEEEECCCEECCCHHHHHHHHHHHHCCCHHHHHH
YVEASEEVLRKNIFAQYEAYMSRLAYNMPRTIYIGSFETFGGATCNVAYKREVLEEVGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECHHHHHHHHHHHCCC
DETFPVAAGEDADLKLRVALKGYKFAFIPLKAIHIQDYTLKGFWKQQVNRGIGNYYFRKK
CCCCCCCCCCCCCEEEEEEEECCEEEEEEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHH
WQSFFNKDELKNIRAAPNYNIPRMILKDGKLLMLALFLIAVIANRYGQIKARKILKKEEE
HHHHHCHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
K
C
>Mature Secondary Structure
MKAHVSIVVPTYNRKKKLQQCLKALINQNYPKERYEIVVVDDGSTDGTYEFLQETRKEIQ
CCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
NLRVLRQRNKGPAAARNLGIKNAQGEIVFFVDDDVIVPNNWIKEFLNVFRKYPEVAAVSG
HHHHHHHCCCCCHHHHHCCCCCCCCCEEEEECCCEECCCHHHHHHHHHHHHCCCHHHHHH
YVEASEEVLRKNIFAQYEAYMSRLAYNMPRTIYIGSFETFGGATCNVAYKREVLEEVGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECHHHHHHHHHHHCCC
DETFPVAAGEDADLKLRVALKGYKFAFIPLKAIHIQDYTLKGFWKQQVNRGIGNYYFRKK
CCCCCCCCCCCCCEEEEEEEECCEEEEEEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHH
WQSFFNKDELKNIRAAPNYNIPRMILKDGKLLMLALFLIAVIANRYGQIKARKILKKEEE
HHHHHCHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]