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The map label for this gene is vhuA [H]

Identifier: 18977704

GI number: 18977704

Start: 1252604

End: 1253842

Strand: Direct

Name: vhuA [H]

Synonym: PF1332

Alternate gene names: 18977704

Gene position: 1252604-1253842 (Clockwise)

Preceding gene: 18977703

Following gene: 33359560

Centisome position: 65.64

GC content: 44.79

Gene sequence:

>1239_bases
ATGATAATCGAGCTTGATGAGTTTACTAGGGTAGAGGGGAATGGAAAGGCAGAGATAGTAATTGAAAACGGTGAGGTTAA
GGATGCTAGGGTAAAAATCGTAGAAGGCCCAAGGTTTTTTGAGATTTTGACCTTGGGAAGAGATTATTGGGATGTTCCAG
ATTTAGAGGCCAGAATATGCGCCATCTGTTACATAGCTCATTCGGTGGCCTCTGTTAGAGCAATAGAAAAGGCCCTAGGA
ATAGATGTTCCCGAGAGCGTGGAAAAGCTTAGGGAATTAGCTCTTTGGGGAGAGATAATTGAGAGCCATGCACTCCACCT
CTACCTCCTTGCCCTCCCAGATGTCTTTGGCTACCCAGATGCTATTTCCATGATACCGAGGCATGGAGAGTTAGTTAAGG
AAGGTTTGACAATAAAAGCGTTTGGAAATGCCATCAGAGAGCTAATTGGTGGGAGGGAAATTCACGGAATAAACATAAAG
CCTGGAGGTTTTGGAAGGTATCCGAGTGAAGAGGAACTTGAAAAGATTGCCGAGCATTCAAAATCTTTAATAAAGTTTGC
CAGGAGGATAGTTGGAATCTTCGCTTCCCAGGAGGCGGGAGGAGCTGTTGGTGAAGTTCTAATGGCTACTTCAGACTACT
TGTGGGGTGATGAACTAATCATTAATGGAGAGAGAGTCCAATATTATGAAGTGGACGAGGTTCCAGTCGGATACAGCTTT
GCAAAGCACTCTTACTACAAAGGAAACCCCGTGTTTGTCGGTGCTCTTCCCAGGTTGCTCTTAAAGGGAGAGTCAATAGA
AGGAGAAGCGGCGAGAATGTTGGAGGAGTATAGGGATAAGTTGGAGAGCAAGTATGTAATCTACAACAATCTGGCCCAGG
CCATAGAACTTCTCTATGCCTTGGAGAGAGTTCCCCAATTAGTTGAAGAAATACTCAGTGAAGGCATTGAAAGAGGAAAT
GGTGAGATTTCCCAGGAGAGCGGAGAAGGAGTTGGATATGTGGAGGCCCCAAGGGGAGTCTTAGTTCACCACTACAGAAT
TGAGAACGGTAAAGTTGTGTGGTCAAATACAATAACTCCAACGGCATTCAACCAGCGCTTAATGGAGTTAAGCCTTCTAG
AGGAGGCAAAGAGGCTTTATGGCTCTGAGTCTGAAGAAAATATGAAGAAGAGACTTGAGGTAATTGTAAGGGCCTTTGAC
CCGTGTATCTCCTGTTCAGTGCACTTCGTCAAGCTTTAA

Upstream 100 bases:

>100_bases
CTTTGGACAACAACGTTCCTGGAATGTTCGAAGTTCTTAAGGAAATTCTTCCTGATGAGGAGATTGCTAGGAAACTAAGG
ACTTTTGCGAGGTGGTAGTT

Downstream 100 bases:

>100_bases
ATTAACATCTTTATTTTTTCAAGGACTTCCCTTGGATTTCTACCAAACACCAATATCATTGGCTCCTTTCCTACATCCCC
TAGGTGATAGATGATGTCGG

Product: H-II alpha (hydrogenase subunit alpha)

Products: 2 Oxidized Ferredoxin; H(2). [C]

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MIIELDEFTRVEGNGKAEIVIENGEVKDARVKIVEGPRFFEILTLGRDYWDVPDLEARICAICYIAHSVASVRAIEKALG
IDVPESVEKLRELALWGEIIESHALHLYLLALPDVFGYPDAISMIPRHGELVKEGLTIKAFGNAIRELIGGREIHGINIK
PGGFGRYPSEEELEKIAEHSKSLIKFARRIVGIFASQEAGGAVGEVLMATSDYLWGDELIINGERVQYYEVDEVPVGYSF
AKHSYYKGNPVFVGALPRLLLKGESIEGEAARMLEEYRDKLESKYVIYNNLAQAIELLYALERVPQLVEEILSEGIERGN
GEISQESGEGVGYVEAPRGVLVHHYRIENGKVVWSNTITPTAFNQRLMELSLLEEAKRLYGSESEENMKKRLEVIVRAFD
PCISCSVHFVKL

Sequences:

>Translated_412_residues
MIIELDEFTRVEGNGKAEIVIENGEVKDARVKIVEGPRFFEILTLGRDYWDVPDLEARICAICYIAHSVASVRAIEKALG
IDVPESVEKLRELALWGEIIESHALHLYLLALPDVFGYPDAISMIPRHGELVKEGLTIKAFGNAIRELIGGREIHGINIK
PGGFGRYPSEEELEKIAEHSKSLIKFARRIVGIFASQEAGGAVGEVLMATSDYLWGDELIINGERVQYYEVDEVPVGYSF
AKHSYYKGNPVFVGALPRLLLKGESIEGEAARMLEEYRDKLESKYVIYNNLAQAIELLYALERVPQLVEEILSEGIERGN
GEISQESGEGVGYVEAPRGVLVHHYRIENGKVVWSNTITPTAFNQRLMELSLLEEAKRLYGSESEENMKKRLEVIVRAFD
PCISCSVHFVKL
>Mature_412_residues
MIIELDEFTRVEGNGKAEIVIENGEVKDARVKIVEGPRFFEILTLGRDYWDVPDLEARICAICYIAHSVASVRAIEKALG
IDVPESVEKLRELALWGEIIESHALHLYLLALPDVFGYPDAISMIPRHGELVKEGLTIKAFGNAIRELIGGREIHGINIK
PGGFGRYPSEEELEKIAEHSKSLIKFARRIVGIFASQEAGGAVGEVLMATSDYLWGDELIINGERVQYYEVDEVPVGYSF
AKHSYYKGNPVFVGALPRLLLKGESIEGEAARMLEEYRDKLESKYVIYNNLAQAIELLYALERVPQLVEEILSEGIERGN
GEISQESGEGVGYVEAPRGVLVHHYRIENGKVVWSNTITPTAFNQRLMELSLLEEAKRLYGSESEENMKKRLEVIVRAFD
PCISCSVHFVKL

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=204, Percent_Identity=30.3921568627451, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789368, Length=287, Percent_Identity=25.4355400696864, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.99.6 [C]

Molecular weight: Translated: 46181; Mature: 46181

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIELDEFTRVEGNGKAEIVIENGEVKDARVKIVEGPRFFEILTLGRDYWDVPDLEARIC
CEEECCCHHEECCCCCEEEEEECCCCCCCEEEEECCCCEEEHEECCCCCCCCCCHHHHHH
AICYIAHSVASVRAIEKALGIDVPESVEKLRELALWGEIIESHALHLYLLALPDVFGYPD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCC
AISMIPRHGELVKEGLTIKAFGNAIRELIGGREIHGINIKPGGFGRYPSEEELEKIAEHS
HHHHCCCCHHHHHCCCEEEHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
KSLIKFARRIVGIFASQEAGGAVGEVLMATSDYLWGDELIINGERVQYYEVDEVPVGYSF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCCCCCCHH
AKHSYYKGNPVFVGALPRLLLKGESIEGEAARMLEEYRDKLESKYVIYNNLAQAIELLYA
HHCCEECCCCEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHH
LERVPQLVEEILSEGIERGNGEISQESGEGVGYVEAPRGVLVHHYRIENGKVVWSNTITP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEEEEECCCEEEEECCCCC
TAFNQRLMELSLLEEAKRLYGSESEENMKKRLEVIVRAFDPCISCSVHFVKL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHCCCEEEEEC
>Mature Secondary Structure
MIIELDEFTRVEGNGKAEIVIENGEVKDARVKIVEGPRFFEILTLGRDYWDVPDLEARIC
CEEECCCHHEECCCCCEEEEEECCCCCCCEEEEECCCCEEEHEECCCCCCCCCCHHHHHH
AICYIAHSVASVRAIEKALGIDVPESVEKLRELALWGEIIESHALHLYLLALPDVFGYPD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCC
AISMIPRHGELVKEGLTIKAFGNAIRELIGGREIHGINIKPGGFGRYPSEEELEKIAEHS
HHHHCCCCHHHHHCCCEEEHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
KSLIKFARRIVGIFASQEAGGAVGEVLMATSDYLWGDELIINGERVQYYEVDEVPVGYSF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCCCCCCHH
AKHSYYKGNPVFVGALPRLLLKGESIEGEAARMLEEYRDKLESKYVIYNNLAQAIELLYA
HHCCEECCCCEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHH
LERVPQLVEEILSEGIERGNGEISQESGEGVGYVEAPRGVLVHHYRIENGKVVWSNTITP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEEEEECCCEEEEECCCCC
TAFNQRLMELSLLEEAKRLYGSESEENMKKRLEVIVRAFDPCISCSVHFVKL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: Ni; Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 Reduced Ferredoxin; 2 H(+) [C]

Specific reaction: 2 Reduced Ferredoxin + 2 H(+) = 2 Oxidized Ferredoxin + H(2). [C]

General reaction: Redox reaction [C]

Inhibitor: Dimethyl sulfoxide; EDTA; NaCl; Tiron; Urea [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]