Click here to switch to the map view.

The map label for this gene is rfaE [C]

Identifier: 18977488

GI number: 18977488

Start: 1063621

End: 1064067

Strand: Direct

Name: rfaE [C]

Synonym: PF1116

Alternate gene names: 18977488

Gene position: 1063621-1064067 (Clockwise)

Preceding gene: 18977487

Following gene: 33359542

Centisome position: 55.74

GC content: 43.4

Gene sequence:

>447_bases
ATGGGAGATAACCGCAAGGTAAGGGTTGTGGTGGGAGGTGTTTTCGACATCCTCCACGTCGGCCATGTCCACTTTTTGAA
GATGGCTAAGGAGTTGGGTGATGAGTTAATAGTTATTGTTGCCCATGATGAAACCGTGAAGAGGAGAAAAGGAAGACCTC
CTATTAATCCAGCTGAAGACAGGGCAGAACTGCTCAAGAGTATTAGGTATGTGGATGATGTTGTGATTGGAGAACCGGGT
GAAATAAGTATAGATTTGATAAAAAGGCTGAAGCCGGACGTAATAGCCTTGGGACCAGATCAAGATTTTAGTTGCGAGGA
GTTAAAGAAGAGGCTCAGAAAAGAGGGAATAAATGCGGAGGTTATCAGGTTGCCCTACTTGTATAAGTCCGATAGGGCAA
AGACAAGTAAGATAATTCAGAGAATAATCGAGACGTTTTGTGAATGA

Upstream 100 bases:

>100_bases
GACCTCTAGCCATTGGAATAGCACTCATGAGTGGAAAAGCAATGAAAGAGAAGAACAGGGGAAAGGCAGTGAAAGTCATC
CACCACGCGAGGGATAAGAT

Downstream 100 bases:

>100_bases
AGTCCAAACTTTCAATTCTTTTGTAGTCTTATTGGAACCGGAGGCTCTACCTAATCATCCTCTTGACACAACTCCTTTCA
ATTCTTTTGTAGTCTTATTG

Product: glycerol-3-phosphate cytidyltransferase

Products: diphosphate; CDPglycerol

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MGDNRKVRVVVGGVFDILHVGHVHFLKMAKELGDELIVIVAHDETVKRRKGRPPINPAEDRAELLKSIRYVDDVVIGEPG
EISIDLIKRLKPDVIALGPDQDFSCEELKKRLRKEGINAEVIRLPYLYKSDRAKTSKIIQRIIETFCE

Sequences:

>Translated_148_residues
MGDNRKVRVVVGGVFDILHVGHVHFLKMAKELGDELIVIVAHDETVKRRKGRPPINPAEDRAELLKSIRYVDDVVIGEPG
EISIDLIKRLKPDVIALGPDQDFSCEELKKRLRKEGINAEVIRLPYLYKSDRAKTSKIIQRIIETFCE
>Mature_147_residues
GDNRKVRVVVGGVFDILHVGHVHFLKMAKELGDELIVIVAHDETVKRRKGRPPINPAEDRAELLKSIRYVDDVVIGEPGE
ISIDLIKRLKPDVIALGPDQDFSCEELKKRLRKEGINAEVIRLPYLYKSDRAKTSKIIQRIIETFCE

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0615

COG function: function code MI; Cytidylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4505651, Length=99, Percent_Identity=35.3535353535354, Blast_Score=71, Evalue=3e-13,
Organism=Homo sapiens, GI296841136, Length=99, Percent_Identity=35.3535353535354, Blast_Score=71, Evalue=4e-13,
Organism=Escherichia coli, GI1789432, Length=135, Percent_Identity=38.5185185185185, Blast_Score=72, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193203447, Length=91, Percent_Identity=38.4615384615385, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17509873, Length=91, Percent_Identity=38.4615384615385, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533408, Length=132, Percent_Identity=31.8181818181818, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI193209647, Length=134, Percent_Identity=31.3432835820896, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI45550974, Length=102, Percent_Identity=36.2745098039216, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI45550975, Length=102, Percent_Identity=36.2745098039216, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI45550973, Length=102, Percent_Identity=36.2745098039216, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19921220, Length=91, Percent_Identity=40.6593406593407, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2 [H]

EC number: 2.7.7.39

Molecular weight: Translated: 16852; Mature: 16721

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDNRKVRVVVGGVFDILHVGHVHFLKMAKELGDELIVIVAHDETVKRRKGRPPINPAED
CCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHCCCCCCCCHHH
RAELLKSIRYVDDVVIGEPGEISIDLIKRLKPDVIALGPDQDFSCEELKKRLRKEGINAE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCEE
VIRLPYLYKSDRAKTSKIIQRIIETFCE
EEEECCHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GDNRKVRVVVGGVFDILHVGHVHFLKMAKELGDELIVIVAHDETVKRRKGRPPINPAED
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHCCCCCCCCHHH
RAELLKSIRYVDDVVIGEPGEISIDLIKRLKPDVIALGPDQDFSCEELKKRLRKEGINAE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCEE
VIRLPYLYKSDRAKTSKIIQRIIETFCE
EEEECCHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; sn-glycerol 3-phosphate

Specific reaction: CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]