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The map label for this gene is leuD [H]

Identifier: 18977311

GI number: 18977311

Start: 903829

End: 904323

Strand: Direct

Name: leuD [H]

Synonym: PF0939

Alternate gene names: 18977311

Gene position: 903829-904323 (Clockwise)

Preceding gene: 18977310

Following gene: 18977312

Centisome position: 47.36

GC content: 45.05

Gene sequence:

>495_bases
ATGAAGGCTAGAGGGAGGGCCTGGAAGTATGGGGACAATATAGACACCGACGTTATAATTCCAGCAAGGTACTTGAACAC
ATCAGATCCAAAAGAGCTGGCCCAGCATGTTCTTGAAGACCTAGACCCTGAGTTTAGGTACAAGATGAAGCCTGGAGATA
TAATAGTGGCTGGAGAAAACTTCGGATGTGGGAGCTCAAGAGAACATGCTCCATTGGCAATTAAAGCGGCGGGAGTGTCT
GCAGTTATAGCGAAGTCTTTCGCTAGGATATTCTATAGGAATGCAATAAACATTGGGCTACCAATTTTGGAGGCTCCTGA
GGCTGTTGAAAGGATAGAGACTGGAGATGAGATCGAAATAGACTTCTCAACTGGAGAAATCAGAAATCTGACAAAGGGGG
AAGTATATCACGCCAACCCATTTCCCGAATTTATAATGGAAATCATCAAGGCTGGAGGGCTTGTTGAATGGGCTAAGAGG
AGGTTGGCAAAATGA

Upstream 100 bases:

>100_bases
GAATGGGTCACCCAAAGAGCGAAGTTTACTTGGCAAGTCCCTATGTAGCTGCAGCTTCGGCTATTCTTGGTAGAATTGCC
TCTCCAGAGGAGGTGGTAAA

Downstream 100 bases:

>100_bases
TAAAGATAGCTGTGATCCCAGGTGATGGGATAGGAAAGGAGGTAGTGGCTGAGGGGCTAAAGGTTCTTCGCAAAATAGAG
GAGCTGAGCAACGTTAAGTT

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKARGRAWKYGDNIDTDVIIPARYLNTSDPKELAQHVLEDLDPEFRYKMKPGDIIVAGENFGCGSSREHAPLAIKAAGVS
AVIAKSFARIFYRNAINIGLPILEAPEAVERIETGDEIEIDFSTGEIRNLTKGEVYHANPFPEFIMEIIKAGGLVEWAKR
RLAK

Sequences:

>Translated_164_residues
MKARGRAWKYGDNIDTDVIIPARYLNTSDPKELAQHVLEDLDPEFRYKMKPGDIIVAGENFGCGSSREHAPLAIKAAGVS
AVIAKSFARIFYRNAINIGLPILEAPEAVERIETGDEIEIDFSTGEIRNLTKGEVYHANPFPEFIMEIIKAGGLVEWAKR
RLAK
>Mature_164_residues
MKARGRAWKYGDNIDTDVIIPARYLNTSDPKELAQHVLEDLDPEFRYKMKPGDIIVAGENFGCGSSREHAPLAIKAAGVS
AVIAKSFARIFYRNAINIGLPILEAPEAVERIETGDEIEIDFSTGEIRNLTKGEVYHANPFPEFIMEIIKAGGLVEWAKR
RLAK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=136, Percent_Identity=34.5588235294118, Blast_Score=74, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320440, Length=174, Percent_Identity=37.3563218390805, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=103, Percent_Identity=37.8640776699029, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011824
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 18257; Mature: 18257

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKARGRAWKYGDNIDTDVIIPARYLNTSDPKELAQHVLEDLDPEFRYKMKPGDIIVAGEN
CCCCCCCEECCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEECCC
FGCGSSREHAPLAIKAAGVSAVIAKSFARIFYRNAINIGLPILEAPEAVERIETGDEIEI
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEE
DFSTGEIRNLTKGEVYHANPFPEFIMEIIKAGGLVEWAKRRLAK
EECCCCCCCCCCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MKARGRAWKYGDNIDTDVIIPARYLNTSDPKELAQHVLEDLDPEFRYKMKPGDIIVAGEN
CCCCCCCEECCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEECCC
FGCGSSREHAPLAIKAAGVSAVIAKSFARIFYRNAINIGLPILEAPEAVERIETGDEIEI
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEE
DFSTGEIRNLTKGEVYHANPFPEFIMEIIKAGGLVEWAKRRLAK
EECCCCCCCCCCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]