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The map label for this gene is leuC1

Identifier: 18977310

GI number: 18977310

Start: 902558

End: 903832

Strand: Direct

Name: leuC1

Synonym: PF0938

Alternate gene names: 18977310

Gene position: 902558-903832 (Clockwise)

Preceding gene: 18977309

Following gene: 18977311

Centisome position: 47.3

GC content: 45.18

Gene sequence:

>1275_bases
ATGGGCGGAATGACAATAGCTGAAAAAATTTTGGCAACTCATGCCGGAAAGGAAGAGGTAAAACCTGGAGAGATAGTTTT
GGCAAAAGTGGACTTCATGTTTGGGAATGATGTAACAACCCCCTTAGCCATAAAAACGTTTAGAAAGATTGGAGTGGAGA
AGGTTTTTGACCCAGAAAGAATAGCAATAGTTTTAGATCACTTCACTCCAAATAAAGATATCAAAGCTGCGGAGCAATGT
AAATTCTCACGAGAATTTGCCAGAGAACAGGGAATCAAGTGGTTCTTTGAGGGAGGAAACGTTGGTGTCGAGCATTGTTT
ACTCCCTGAGTTAGGATTAGTTCTCCCAGGAGAGCTAATTATTGGAGCAGATTCCCACACTTGCACTTATGGAGCCCTGG
GTGCCTTTGCAACTGGAGTTGGTAGTACTGACTTAGCAGTTGCAATGGCAACAGGAGAGGCGTGGTTCAGGGTTCCCGAA
ACGATAAAATTCGTTTATGAAGGAGACCTTCAACCCTGGGTAACTAGTAAGGATCTCATACTTTACACAATCGGAGACAT
AGGGGTTAATGGAGCCCTCTACAAAGTCATGGAATTTTCGGGAGAAGTTATAGAGAAGCTTTCCGTGGAACAGAGGATGA
CGATGACTAATATGGCCATAGAGGCCGGAGCAAAGACTGGGATAATAGAGCCGGATAAGAAGACTATAGAGTACGTAAAG
GGGAGGGCCAAGAGAGAGTACAAGATCTACAAGAGCGATGAAGATGCAAAGTACTACAAGGTTATTGAGTATGACGTTAG
TAAAATTGAACCTCAAGTTGCCTTTCCACACTTACCCGAAAACACGGTTCCCATCAGCAAGGCCGCAAAGATGAACATAA
AGATAGACCAAGTTGTCATTGGCTCATGTACGAATGGTAGGTTGGAAGACCTTAGAATGGCCGCTGAAGTTTTAGAAGGC
CAGAAAGTGGCCCCCTGGGTAAGGCTTATCATTCTTCCATGTTCACCGACTGTGTATTTCAAGGCAATGAAGGAGGGCCT
CTTGGAGATTTTCCTGGAGGCAGGAGCTGTTATAGGGCCTCCAACTTGTGGACCGTGCCTAGGAGGTCACATGGGTATTC
TGGCGAGTGGTGAGAGAGCTGTATCTACAACAAACAGGAACTTTGTTGGAAGAATGGGTCACCCAAAGAGCGAAGTTTAC
TTGGCAAGTCCCTATGTAGCTGCAGCTTCGGCTATTCTTGGTAGAATTGCCTCTCCAGAGGAGGTGGTAAAATGA

Upstream 100 bases:

>100_bases
AACGGAGAGATTTACGTTGGAAGAGGTTTGAGCACCGACATTATTGAGGCAAGTGCTCAGGCGTATCTTTCTGCTCTAAA
TAGAATTAGGAGGTGAAATT

Downstream 100 bases:

>100_bases
AGGCTAGAGGGAGGGCCTGGAAGTATGGGGACAATATAGACACCGACGTTATAATTCCAGCAAGGTACTTGAACACATCA
GATCCAAAAGAGCTGGCCCA

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MGGMTIAEKILATHAGKEEVKPGEIVLAKVDFMFGNDVTTPLAIKTFRKIGVEKVFDPERIAIVLDHFTPNKDIKAAEQC
KFSREFAREQGIKWFFEGGNVGVEHCLLPELGLVLPGELIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPE
TIKFVYEGDLQPWVTSKDLILYTIGDIGVNGALYKVMEFSGEVIEKLSVEQRMTMTNMAIEAGAKTGIIEPDKKTIEYVK
GRAKREYKIYKSDEDAKYYKVIEYDVSKIEPQVAFPHLPENTVPISKAAKMNIKIDQVVIGSCTNGRLEDLRMAAEVLEG
QKVAPWVRLIILPCSPTVYFKAMKEGLLEIFLEAGAVIGPPTCGPCLGGHMGILASGERAVSTTNRNFVGRMGHPKSEVY
LASPYVAAASAILGRIASPEEVVK

Sequences:

>Translated_424_residues
MGGMTIAEKILATHAGKEEVKPGEIVLAKVDFMFGNDVTTPLAIKTFRKIGVEKVFDPERIAIVLDHFTPNKDIKAAEQC
KFSREFAREQGIKWFFEGGNVGVEHCLLPELGLVLPGELIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPE
TIKFVYEGDLQPWVTSKDLILYTIGDIGVNGALYKVMEFSGEVIEKLSVEQRMTMTNMAIEAGAKTGIIEPDKKTIEYVK
GRAKREYKIYKSDEDAKYYKVIEYDVSKIEPQVAFPHLPENTVPISKAAKMNIKIDQVVIGSCTNGRLEDLRMAAEVLEG
QKVAPWVRLIILPCSPTVYFKAMKEGLLEIFLEAGAVIGPPTCGPCLGGHMGILASGERAVSTTNRNFVGRMGHPKSEVY
LASPYVAAASAILGRIASPEEVVK
>Mature_423_residues
GGMTIAEKILATHAGKEEVKPGEIVLAKVDFMFGNDVTTPLAIKTFRKIGVEKVFDPERIAIVLDHFTPNKDIKAAEQCK
FSREFAREQGIKWFFEGGNVGVEHCLLPELGLVLPGELIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPET
IKFVYEGDLQPWVTSKDLILYTIGDIGVNGALYKVMEFSGEVIEKLSVEQRMTMTNMAIEAGAKTGIIEPDKKTIEYVKG
RAKREYKIYKSDEDAKYYKVIEYDVSKIEPQVAFPHLPENTVPISKAAKMNIKIDQVVIGSCTNGRLEDLRMAAEVLEGQ
KVAPWVRLIILPCSPTVYFKAMKEGLLEIFLEAGAVIGPPTCGPCLGGHMGILASGERAVSTTNRNFVGRMGHPKSEVYL
ASPYVAAASAILGRIASPEEVVK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=454, Percent_Identity=28.1938325991189, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI8659555, Length=459, Percent_Identity=24.400871459695, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI41352693, Length=425, Percent_Identity=24.7058823529412, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=36.3440860215054, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI87081781, Length=460, Percent_Identity=25.4347826086956, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI2367097, Length=463, Percent_Identity=27.645788336933, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787531, Length=360, Percent_Identity=25.2777777777778, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25149337, Length=448, Percent_Identity=28.5714285714286, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=411, Percent_Identity=28.9537712895377, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=282, Percent_Identity=28.3687943262411, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=446, Percent_Identity=24.6636771300448, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=458, Percent_Identity=35.1528384279476, Blast_Score=232, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6320440, Length=454, Percent_Identity=33.7004405286344, Blast_Score=229, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6323335, Length=449, Percent_Identity=30.9576837416481, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322261, Length=458, Percent_Identity=28.3842794759825, Blast_Score=160, Evalue=4e-40,
Organism=Drosophila melanogaster, GI28571643, Length=451, Percent_Identity=29.7117516629712, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI281365315, Length=450, Percent_Identity=27.5555555555556, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17864292, Length=450, Percent_Identity=27.5555555555556, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI161076999, Length=413, Percent_Identity=28.0871670702179, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI17137564, Length=377, Percent_Identity=25.7294429708223, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24645686, Length=467, Percent_Identity=25.2676659528908, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC1_PYRFU (Q8U2A1)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578667.1
- ProteinModelPortal:   Q8U2A1
- EnsemblBacteria:   EBPYRT00000004263
- GeneID:   1468802
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0938
- NMPDR:   fig|186497.1.peg.973
- GeneTree:   EBGT00050000022452
- HOGENOM:   HBG330745
- OMA:   TDPRDFL
- ProtClustDB:   PRK00402
- BRENDA:   4.2.1.33
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086
- TIGRFAMs:   TIGR02083

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 46211; Mature: 46080

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGMTIAEKILATHAGKEEVKPGEIVLAKVDFMFGNDVTTPLAIKTFRKIGVEKVFDPER
CCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCHHHCCCCCE
IAIVLDHFTPNKDIKAAEQCKFSREFAREQGIKWFFEGGNVGVEHCLLPELGLVLPGELI
EEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCCCCCEE
IGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETIKFVYEGDLQPWVTSKDLI
EECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHEECCCEEEEEEECCCCCCCCCCCEE
LYTIGDIGVNGALYKVMEFSGEVIEKLSVEQRMTMTNMAIEAGAKTGIIEPDKKTIEYVK
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
GRAKREYKIYKSDEDAKYYKVIEYDVSKIEPQVAFPHLPENTVPISKAAKMNIKIDQVVI
CCCCCEEEEEECCCCCCEEEEEEECHHHCCCCCCCCCCCCCCCCCCHHEEEEEEEEEEEE
GSCTNGRLEDLRMAAEVLEGQKVAPWVRLIILPCSPTVYFKAMKEGLLEIFLEAGAVIGP
ECCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECC
PTCGPCLGGHMGILASGERAVSTTNRNFVGRMGHPKSEVYLASPYVAAASAILGRIASPE
CCCCCCCCCCCCEEECCCCEEEECCCCEEECCCCCCCCEEEECCHHHHHHHHHHCCCCHH
EVVK
HHCC
>Mature Secondary Structure 
GGMTIAEKILATHAGKEEVKPGEIVLAKVDFMFGNDVTTPLAIKTFRKIGVEKVFDPER
CCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCHHHCCCCCE
IAIVLDHFTPNKDIKAAEQCKFSREFAREQGIKWFFEGGNVGVEHCLLPELGLVLPGELI
EEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCCCCCEE
IGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETIKFVYEGDLQPWVTSKDLI
EECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHEECCCEEEEEEECCCCCCCCCCCEE
LYTIGDIGVNGALYKVMEFSGEVIEKLSVEQRMTMTNMAIEAGAKTGIIEPDKKTIEYVK
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
GRAKREYKIYKSDEDAKYYKVIEYDVSKIEPQVAFPHLPENTVPISKAAKMNIKIDQVVI
CCCCCEEEEEECCCCCCEEEEEEECHHHCCCCCCCCCCCCCCCCCCHHEEEEEEEEEEEE
GSCTNGRLEDLRMAAEVLEGQKVAPWVRLIILPCSPTVYFKAMKEGLLEIFLEAGAVIGP
ECCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECC
PTCGPCLGGHMGILASGERAVSTTNRNFVGRMGHPKSEVYLASPYVAAASAILGRIASPE
CCCCCCCCCCCCEEECCCCEEEECCCCEEECCCCCCCCEEEECCHHHHHHHHHHCCCCHH
EVVK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA