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The map label for this gene is dphB

Identifier: 18976967

GI number: 18976967

Start: 616202

End: 617011

Strand: Reverse

Name: dphB

Synonym: PF0595

Alternate gene names: 18976967

Gene position: 617011-616202 (Counterclockwise)

Preceding gene: 18976968

Following gene: 18976966

Centisome position: 32.33

GC content: 43.83

Gene sequence:

>810_bases
ATGCCCCTATACTTTATTGGACTTGGGTTATATGATGAGAAAGACATAACGCTCAAAGGATTGGAGATTGCAAAAAGGTG
CGATAAGGTGTATGCAGAATTCTACACCTCTCTTATGGCTGGAACTACGCTTGAGAAAATCGAAGAGGTAATTGGGAAAA
AGATAATAGTCCTCAATAGAGAGGATGTGGAAATGAACTTCGAAAAAATAGTCCTCCCAGAGGCCAAGGAAAAAGAAGTA
GCATTCCTAACCGCTGGGGATCCCATGGTGGCTACTACCCACGCAGAGCTGAGGATAAGGGCGAAGAGAATGGGTGTGGA
GAGCTATGTTATTCACGCCCCAAGCATTTATTCAGCTGTGGCAATAACTGGATTGCATATATATAAGTTTGGAAAGAGTG
CCACAGTAGCCTATCCAGAGGGGAATTGGTTCCCCACTAGCTACTACGATGTAGTTAAAGAGAACTTAGAGAGAGGACTT
CATACTCTCCTCTTCCTTGACATAAAGGCCGAGAAGGGGAAGTACATGACCGCAAATGAGGCCATGGAACTCCTACTTAA
GGTAGAGGAAATGAGGGGAGAGAATGTTTTCACTCCCAACACTTTGGTGGTTGTCCTTGGAAGGGCTGGCTCGCTCAACC
CAACAATTAGGGCTGGATACGTAAAAGATATGATAAAGGAAGACTTTGGAAAGCAACCTCATGTTCTAATAGTTCCAGGG
AAACTTCACGTTGTAGAGGCAGAGTATTTAGTTGAGATTGCAGGTGCCCCAAAAGAGATACTTGAGCAATTTGCTCCTAG
AAAAATGTAA

Upstream 100 bases:

>100_bases
CAATCTTTGGCCACGCGCAGTCATTTGCGAAGATATTAAATGGGTTTGGGTATGAGAGCAGGTCGATATCGAGGGGAGAA
GAAATTAGGAGGTGGATTTA

Downstream 100 bases:

>100_bases
AGTGGAGCAAGAAAAATTTAGAAAGCTTTTCATGTAACTTCATTATTTTCCGACGAAAGGGTTATTAAAATCCCAGGTGA
GTTAAATTTGGTGATGAAGA

Product: diphthine synthase

Products: NA

Alternate protein names: Diphthamide biosynthesis methyltransferase

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MPLYFIGLGLYDEKDITLKGLEIAKRCDKVYAEFYTSLMAGTTLEKIEEVIGKKIIVLNREDVEMNFEKIVLPEAKEKEV
AFLTAGDPMVATTHAELRIRAKRMGVESYVIHAPSIYSAVAITGLHIYKFGKSATVAYPEGNWFPTSYYDVVKENLERGL
HTLLFLDIKAEKGKYMTANEAMELLLKVEEMRGENVFTPNTLVVVLGRAGSLNPTIRAGYVKDMIKEDFGKQPHVLIVPG
KLHVVEAEYLVEIAGAPKEILEQFAPRKM

Sequences:

>Translated_269_residues
MPLYFIGLGLYDEKDITLKGLEIAKRCDKVYAEFYTSLMAGTTLEKIEEVIGKKIIVLNREDVEMNFEKIVLPEAKEKEV
AFLTAGDPMVATTHAELRIRAKRMGVESYVIHAPSIYSAVAITGLHIYKFGKSATVAYPEGNWFPTSYYDVVKENLERGL
HTLLFLDIKAEKGKYMTANEAMELLLKVEEMRGENVFTPNTLVVVLGRAGSLNPTIRAGYVKDMIKEDFGKQPHVLIVPG
KLHVVEAEYLVEIAGAPKEILEQFAPRKM
>Mature_268_residues
PLYFIGLGLYDEKDITLKGLEIAKRCDKVYAEFYTSLMAGTTLEKIEEVIGKKIIVLNREDVEMNFEKIVLPEAKEKEVA
FLTAGDPMVATTHAELRIRAKRMGVESYVIHAPSIYSAVAITGLHIYKFGKSATVAYPEGNWFPTSYYDVVKENLERGLH
TLLFLDIKAEKGKYMTANEAMELLLKVEEMRGENVFTPNTLVVVLGRAGSLNPTIRAGYVKDMIKEDFGKQPHVLIVPGK
LHVVEAEYLVEIAGAPKEILEQFAPRKM

Specific function: Required for the methylation step in diphthamide biosynthesis

COG id: COG1798

COG function: function code J; Diphthamide biosynthesis methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diphthine synthase family

Homologues:

Organism=Homo sapiens, GI117190324, Length=267, Percent_Identity=41.5730337078652, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI117190326, Length=268, Percent_Identity=41.4179104477612, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI117190262, Length=268, Percent_Identity=41.4179104477612, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17531369, Length=270, Percent_Identity=39.2592592592593, Blast_Score=177, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6323201, Length=254, Percent_Identity=39.3700787401575, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28572120, Length=276, Percent_Identity=38.0434782608696, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPHB_PYRFU (Q8U377)

Other databases:

- EMBL:   AE009950
- EMBL:   Y12727
- RefSeq:   NP_578324.1
- ProteinModelPortal:   Q8U377
- SMR:   Q8U377
- EnsemblBacteria:   EBPYRT00000005691
- GeneID:   1468438
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0595
- NMPDR:   fig|186497.1.peg.609
- GeneTree:   EBGT00050000023093
- HOGENOM:   HBG460511
- OMA:   YAEFYTA
- ProtClustDB:   PRK04160
- BRENDA:   2.1.1.98
- HAMAP:   MF_01084
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR004551
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- PANTHER:   PTHR10882
- PIRSF:   PIRSF036432
- TIGRFAMs:   TIGR00522

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.98

Molecular weight: Translated: 30218; Mature: 30087

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLYFIGLGLYDEKDITLKGLEIAKRCDKVYAEFYTSLMAGTTLEKIEEVIGKKIIVLNR
CCEEEEEECEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEEC
EDVEMNFEKIVLPEAKEKEVAFLTAGDPMVATTHAELRIRAKRMGVESYVIHAPSIYSAV
CCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEHHHEEEEHHHCCCCCEEEECCHHHHHH
AITGLHIYKFGKSATVAYPEGNWFPTSYYDVVKENLERGLHTLLFLDIKAEKGKYMTANE
HHHHEEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHH
AMELLLKVEEMRGENVFTPNTLVVVLGRAGSLNPTIRAGYVKDMIKEDFGKQPHVLIVPG
HHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
KLHVVEAEYLVEIAGAPKEILEQFAPRKM
EEEEEEHHHHEEECCCHHHHHHHHCCCCC
>Mature Secondary Structure 
PLYFIGLGLYDEKDITLKGLEIAKRCDKVYAEFYTSLMAGTTLEKIEEVIGKKIIVLNR
CEEEEEECEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEEC
EDVEMNFEKIVLPEAKEKEVAFLTAGDPMVATTHAELRIRAKRMGVESYVIHAPSIYSAV
CCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEHHHEEEEHHHCCCCCEEEECCHHHHHH
AITGLHIYKFGKSATVAYPEGNWFPTSYYDVVKENLERGLHTLLFLDIKAEKGKYMTANE
HHHHEEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHH
AMELLLKVEEMRGENVFTPNTLVVVLGRAGSLNPTIRAGYVKDMIKEDFGKQPHVLIVPG
HHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
KLHVVEAEYLVEIAGAPKEILEQFAPRKM
EEEEEEHHHHEEECCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9288929