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The map label for this gene is alaXS [H]

Identifier: 18976800

GI number: 18976800

Start: 435498

End: 435947

Strand: Direct

Name: alaXS [H]

Synonym: PF0428

Alternate gene names: 18976800

Gene position: 435498-435947 (Clockwise)

Preceding gene: 18976799

Following gene: 18976803

Centisome position: 22.82

GC content: 36.22

Gene sequence:

>450_bases
ATGATAGAAATCAGAACTCATACAGCCTTACATGTTCTAAAAGGCGCGGTTGTAAAAGTTTTGGGTGAAGGTGCTAAATG
GACGGCAAGTGTTTATGTAAATGGAAACCACGGAAGATTAACTGTTAAGTTCAACAGAAAACCAACCCATGAAGAAATTA
GAGAAATAGAAAGGCTCGCAAATGAAAAAATCAAGGAGAATGTTCCCATACAAATATATGAACTTTCCAGGGAAGAGGCA
GAAAAAATATTTGGGGATGAAATGTATGATCTCTTTCCAGTTCCAGAACACGTCAGGACTCTTAAAGTTGTAGTCATCGA
AGACTGGAATGTCAATGCATGCAACAAGGAACATACAAAGACAACTGGTGAAATAGGGGAGATAAAAATAAGGAAAGTTA
GGTTCAGAAAATCCAAGGAACTTCTTGAAATAAGTTTTGATGTATTATAA

Upstream 100 bases:

>100_bases
TCTGGGAGAGGAATGATTTAGTCCTTTTTTAAATATTTTTAGGAGATTTGACAACTTAGATTTGGGAATTTATATAAAGC
CTCTTTCAATAGGTTGTAGT

Downstream 100 bases:

>100_bases
AAAAATAACAGAGAAAAGAGGTTACTCTGTCATAGCCTTCATTAATTCCTCTGCATTTTCTGGAGGCTTTGTTGCTAAGC
TGACAAGGACTGTAGCTATG

Product: partial alanyl-tRNA synthetase matches COOH terminus

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-S; Alanyl-tRNA deacylase AlaX-S; PhoAlaX [H]

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MIEIRTHTALHVLKGAVVKVLGEGAKWTASVYVNGNHGRLTVKFNRKPTHEEIREIERLANEKIKENVPIQIYELSREEA
EKIFGDEMYDLFPVPEHVRTLKVVVIEDWNVNACNKEHTKTTGEIGEIKIRKVRFRKSKELLEISFDVL

Sequences:

>Translated_149_residues
MIEIRTHTALHVLKGAVVKVLGEGAKWTASVYVNGNHGRLTVKFNRKPTHEEIREIERLANEKIKENVPIQIYELSREEA
EKIFGDEMYDLFPVPEHVRTLKVVVIEDWNVNACNKEHTKTTGEIGEIKIRKVRFRKSKELLEISFDVL
>Mature_149_residues
MIEIRTHTALHVLKGAVVKVLGEGAKWTASVYVNGNHGRLTVKFNRKPTHEEIREIERLANEKIKENVPIQIYELSREEA
EKIFGDEMYDLFPVPEHVRTLKVVVIEDWNVNACNKEHTKTTGEIGEIKIRKVRFRKSKELLEISFDVL

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged Ser-tRNA(Ala). Has little activity against Gly- tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-S subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 17275; Mature: 17275

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIRTHTALHVLKGAVVKVLGEGAKWTASVYVNGNHGRLTVKFNRKPTHEEIREIERLA
CEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHH
NEKIKENVPIQIYELSREEAEKIFGDEMYDLFPVPEHVRTLKVVVIEDWNVNACNKEHTK
HHHHHCCCCEEEEECCHHHHHHHHCCCHHCCCCCCCHHEEEEEEEEECCCCCCCCCCCCC
TTGEIGEIKIRKVRFRKSKELLEISFDVL
CCCCCCCEEEEEEECCCCHHEEEEEEECC
>Mature Secondary Structure
MIEIRTHTALHVLKGAVVKVLGEGAKWTASVYVNGNHGRLTVKFNRKPTHEEIREIERLA
CEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHH
NEKIKENVPIQIYELSREEAEKIFGDEMYDLFPVPEHVRTLKVVVIEDWNVNACNKEHTK
HHHHHCCCCEEEEECCHHHHHHHHCCCHHCCCCCCCHHEEEEEEEEECCCCCCCCCCCCC
TTGEIGEIKIRKVRFRKSKELLEISFDVL
CCCCCCCEEEEEEECCCCHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]