Click here to switch to the map view.

The map label for this gene is appA [H]

Identifier: 18976729

GI number: 18976729

Start: 370134

End: 372029

Strand: Direct

Name: appA [H]

Synonym: PF0357

Alternate gene names: 18976729

Gene position: 370134-372029 (Clockwise)

Preceding gene: 18976726

Following gene: 18976730

Centisome position: 19.4

GC content: 43.88

Gene sequence:

>1896_bases
ATGAAGAAATACCTTGCTACAGTTTTAGTTGCTGCCCTAGTTTTGAGCAATCTTGGAGTAGTTCTTGCAGCAGAAGAACT
TCCAAGAGAAGAAACACTGTATACCGCCAATAGCTCCCCGCCAACCAATGCAAATCCCCTTCAGGGGAGTAACATAATAC
CAATAGCCGGGTTGGTGTTTGAACCACTGTTTATGTTGAACTTCATGAAAACAGAACTCATTCCTTGGCTTGCAGACTAT
GGAAAGTGGGTAAACAACAATGTATTTGAAGTTAAGCTAAGAGAAGGAACTAAATGGCAAGATGGCAAGTCTTTAACAGC
TAATGATGTTAAGTTCTCATTTGAATACTACGAGAAAGTCGGACTTAAGGACTGGTCAAGGTATGGACTTACTGAGATTA
AAGTTGTAGACGACAGAACAGTACAATTTGTTTTTAGTGGGACTCCAAACGTCTGGGCATGGAGAAACGAGCTTTATAGT
GTTTTAATTCTTCCAGAACACATCTTCAAAGATCTCGATCCCCAACAGGTCACCCAGATGACATTCTTGGGAGCTGAGCA
AAAATACTTGGTTGGTTCAGGGGCATATAAACTTTACAAAGTTGTCGAACAACAAAAGGCAATATTCGTGAGAAACGACG
ATTGGTGGGGTGCGAAGTACTTTGGACTACCAAGTCCCAAGTACATAGTCCAGCTTTACGTTGGTTCAAATGATCAAGCA
GCAAACATGTTCCTTAAGGGGGATCTTGATGTAGGTACTTATTACGTGGATATAGTTGAACTCAAGAAGCAGAACCCCTA
CATAGTCAGCTGGCTCGATAGGCCACCCTACTTCCCACCCGTTGTTCCTGTTATCATGTACTTCAACGTTAAGCACAAGC
CTCTCGACAACCTACTTGTAAGAAAGGCTATAGCTGAGGCAATTTGTCCCTCTCAAATAGTCAAACAAGGTCCAATCAGT
GACCTCCCAGACCAAACTCCACTGGGTAACGTCATGAAGCCATGGAAGGAGAAGATAGGGGTTAGCCAGCTAATTGAGAA
GTATGGATGGAAGTACTGTAACCCAAGTGAAGCCGCTGAACTATTAGACCAAGCTGGAATCAAAGACATTGATGGAGATG
GAATAAGGGAGTATAATGGACAGGATCTTGTTCTAACATTTGCCGCATGTGGAGTATGTAGTGACTGGATGCAGGCTGTA
GAAATTGTAGTTAACCAGCTAAAAGCAGTGGGAATAAAGGTTGACGTCAAGAAGTACGATTGGGGAACAATGGTTAGCAA
GCAACAAGCAGGTGAATTTGATTTAACAATGCACTGGGCTGGAACGTTTAAGCCCGATCCATACAGCGTCTATTACGACC
TCCTCTACTACTACAAGAGTGACCAAGAGAAGGGTGGAGCAAACTTTGGCAACTACTACAATTCAAAGGCAAATGAGTTG
CTCGATGAACTTGCAAAGACACCAGATGAAGCAAAGCAAGTTCAACTTCTTGCCGAGCTGACAAAGATATGGCTCCAAGA
CATTCCAGCAGTCCCACTCTACATGGGTACATTATTCTATGAAGCAAACACCCAATACTGGGCCAACTGGCCAAATGAAG
ACAATCCGTATGGTGTACCCATCTTCTGGCCAGGCTTTGGTACCTGGGGTACAGCTCTAGCCTTCCTGGGAGTTAGACCA
GCAAAAGCAGTAAGCCCAACTACTTCCACAGTTATCCAGGAGAAGACCGAGACAGTTACCCAGGAGAAAACTAAAACAGT
AGTTCAAACCGTTACAGTAACTCAAACTCCAACAGAGACTGCAACGTCACCAACAGAAACTGAAGGAATTTGTGGGCCCG
CAATTTTAGTAGGTCTAGCTGTAGTCCCACTCCTTCTAAGAAGGAGGAGGCGTTGA

Upstream 100 bases:

>100_bases
GGACTTGGCACGTTCAATCCTTACCTAGTTATAACCAAAAAGTTTATATAGGATCTCAATTTTTTATAAATCTGACTAAA
TTATAATGAGGTGACATGCT

Downstream 100 bases:

>100_bases
GTTTCTTCTTTTTCTAAATTTTTCTAGGGGGGAGTGTTATGGGGTTTAAAGAATATCTAAAGAGGAAAATTATTGTTTAC
CTCCTGACGTTTATTTTTGC

Product: dipeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MKKYLATVLVAALVLSNLGVVLAAEELPREETLYTANSSPPTNANPLQGSNIIPIAGLVFEPLFMLNFMKTELIPWLADY
GKWVNNNVFEVKLREGTKWQDGKSLTANDVKFSFEYYEKVGLKDWSRYGLTEIKVVDDRTVQFVFSGTPNVWAWRNELYS
VLILPEHIFKDLDPQQVTQMTFLGAEQKYLVGSGAYKLYKVVEQQKAIFVRNDDWWGAKYFGLPSPKYIVQLYVGSNDQA
ANMFLKGDLDVGTYYVDIVELKKQNPYIVSWLDRPPYFPPVVPVIMYFNVKHKPLDNLLVRKAIAEAICPSQIVKQGPIS
DLPDQTPLGNVMKPWKEKIGVSQLIEKYGWKYCNPSEAAELLDQAGIKDIDGDGIREYNGQDLVLTFAACGVCSDWMQAV
EIVVNQLKAVGIKVDVKKYDWGTMVSKQQAGEFDLTMHWAGTFKPDPYSVYYDLLYYYKSDQEKGGANFGNYYNSKANEL
LDELAKTPDEAKQVQLLAELTKIWLQDIPAVPLYMGTLFYEANTQYWANWPNEDNPYGVPIFWPGFGTWGTALAFLGVRP
AKAVSPTTSTVIQEKTETVTQEKTKTVVQTVTVTQTPTETATSPTETEGICGPAILVGLAVVPLLLRRRRR

Sequences:

>Translated_631_residues
MKKYLATVLVAALVLSNLGVVLAAEELPREETLYTANSSPPTNANPLQGSNIIPIAGLVFEPLFMLNFMKTELIPWLADY
GKWVNNNVFEVKLREGTKWQDGKSLTANDVKFSFEYYEKVGLKDWSRYGLTEIKVVDDRTVQFVFSGTPNVWAWRNELYS
VLILPEHIFKDLDPQQVTQMTFLGAEQKYLVGSGAYKLYKVVEQQKAIFVRNDDWWGAKYFGLPSPKYIVQLYVGSNDQA
ANMFLKGDLDVGTYYVDIVELKKQNPYIVSWLDRPPYFPPVVPVIMYFNVKHKPLDNLLVRKAIAEAICPSQIVKQGPIS
DLPDQTPLGNVMKPWKEKIGVSQLIEKYGWKYCNPSEAAELLDQAGIKDIDGDGIREYNGQDLVLTFAACGVCSDWMQAV
EIVVNQLKAVGIKVDVKKYDWGTMVSKQQAGEFDLTMHWAGTFKPDPYSVYYDLLYYYKSDQEKGGANFGNYYNSKANEL
LDELAKTPDEAKQVQLLAELTKIWLQDIPAVPLYMGTLFYEANTQYWANWPNEDNPYGVPIFWPGFGTWGTALAFLGVRP
AKAVSPTTSTVIQEKTETVTQEKTKTVVQTVTVTQTPTETATSPTETEGICGPAILVGLAVVPLLLRRRRR
>Mature_631_residues
MKKYLATVLVAALVLSNLGVVLAAEELPREETLYTANSSPPTNANPLQGSNIIPIAGLVFEPLFMLNFMKTELIPWLADY
GKWVNNNVFEVKLREGTKWQDGKSLTANDVKFSFEYYEKVGLKDWSRYGLTEIKVVDDRTVQFVFSGTPNVWAWRNELYS
VLILPEHIFKDLDPQQVTQMTFLGAEQKYLVGSGAYKLYKVVEQQKAIFVRNDDWWGAKYFGLPSPKYIVQLYVGSNDQA
ANMFLKGDLDVGTYYVDIVELKKQNPYIVSWLDRPPYFPPVVPVIMYFNVKHKPLDNLLVRKAIAEAICPSQIVKQGPIS
DLPDQTPLGNVMKPWKEKIGVSQLIEKYGWKYCNPSEAAELLDQAGIKDIDGDGIREYNGQDLVLTFAACGVCSDWMQAV
EIVVNQLKAVGIKVDVKKYDWGTMVSKQQAGEFDLTMHWAGTFKPDPYSVYYDLLYYYKSDQEKGGANFGNYYNSKANEL
LDELAKTPDEAKQVQLLAELTKIWLQDIPAVPLYMGTLFYEANTQYWANWPNEDNPYGVPIFWPGFGTWGTALAFLGVRP
AKAVSPTTSTVIQEKTETVTQEKTKTVVQTVTVTQTPTETATSPTETEGICGPAILVGLAVVPLLLRRRRR

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 71175; Mature: 71175

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYLATVLVAALVLSNLGVVLAAEELPREETLYTANSSPPTNANPLQGSNIIPIAGLVF
CHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEHHHHH
EPLFMLNFMKTELIPWLADYGKWVNNNVFEVKLREGTKWQDGKSLTANDVKFSFEYYEKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHC
GLKDWSRYGLTEIKVVDDRTVQFVFSGTPNVWAWRNELYSVLILPEHIFKDLDPQQVTQM
CCCHHHHCCCEEEEEECCCEEEEEECCCCCCEEEHHHHEEEEEECHHHHCCCCHHHHHHH
TFLGAEQKYLVGSGAYKLYKVVEQQKAIFVRNDDWWGAKYFGLPSPKYIVQLYVGSNDQA
HHCCCCCEEEECCCHHHHHHHHHHCCEEEEECCCCCCEEECCCCCCCEEEEEEECCCCCC
ANMFLKGDLDVGTYYVDIVELKKQNPYIVSWLDRPPYFPPVVPVIMYFNVKHKPLDNLLV
CEEEEEECCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHEECCCCCCHHHHHH
RKAIAEAICPSQIVKQGPISDLPDQTPLGNVMKPWKEKIGVSQLIEKYGWKYCNPSEAAE
HHHHHHHCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHH
LLDQAGIKDIDGDGIREYNGQDLVLTFAACGVCSDWMQAVEIVVNQLKAVGIKVDVKKYD
HHHHCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
WGTMVSKQQAGEFDLTMHWAGTFKPDPYSVYYDLLYYYKSDQEKGGANFGNYYNSKANEL
CCHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHH
LDELAKTPDEAKQVQLLAELTKIWLQDIPAVPLYMGTLFYEANTQYWANWPNEDNPYGVP
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCEEECCCCCCCCCCCE
IFWPGFGTWGTALAFLGVRPAKAVSPTTSTVIQEKTETVTQEKTKTVVQTVTVTQTPTET
EEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
ATSPTETEGICGPAILVGLAVVPLLLRRRRR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKYLATVLVAALVLSNLGVVLAAEELPREETLYTANSSPPTNANPLQGSNIIPIAGLVF
CHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEHHHHH
EPLFMLNFMKTELIPWLADYGKWVNNNVFEVKLREGTKWQDGKSLTANDVKFSFEYYEKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHC
GLKDWSRYGLTEIKVVDDRTVQFVFSGTPNVWAWRNELYSVLILPEHIFKDLDPQQVTQM
CCCHHHHCCCEEEEEECCCEEEEEECCCCCCEEEHHHHEEEEEECHHHHCCCCHHHHHHH
TFLGAEQKYLVGSGAYKLYKVVEQQKAIFVRNDDWWGAKYFGLPSPKYIVQLYVGSNDQA
HHCCCCCEEEECCCHHHHHHHHHHCCEEEEECCCCCCEEECCCCCCCEEEEEEECCCCCC
ANMFLKGDLDVGTYYVDIVELKKQNPYIVSWLDRPPYFPPVVPVIMYFNVKHKPLDNLLV
CEEEEEECCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHEECCCCCCHHHHHH
RKAIAEAICPSQIVKQGPISDLPDQTPLGNVMKPWKEKIGVSQLIEKYGWKYCNPSEAAE
HHHHHHHCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHH
LLDQAGIKDIDGDGIREYNGQDLVLTFAACGVCSDWMQAVEIVVNQLKAVGIKVDVKKYD
HHHHCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
WGTMVSKQQAGEFDLTMHWAGTFKPDPYSVYYDLLYYYKSDQEKGGANFGNYYNSKANEL
CCHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHH
LDELAKTPDEAKQVQLLAELTKIWLQDIPAVPLYMGTLFYEANTQYWANWPNEDNPYGVP
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCEEECCCCCCCCCCCE
IFWPGFGTWGTALAFLGVRPAKAVSPTTSTVIQEKTETVTQEKTKTVVQTVTVTQTPTET
EEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
ATSPTETEGICGPAILVGLAVVPLLLRRRRR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]