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The map label for this gene is pckG

Identifier: 18976661

GI number: 18976661

Start: 302436

End: 304310

Strand: Direct

Name: pckG

Synonym: PF0289

Alternate gene names: 18976661

Gene position: 302436-304310 (Clockwise)

Preceding gene: 18976659

Following gene: 18976669

Centisome position: 15.85

GC content: 43.2

Gene sequence:

>1875_bases
ATGATGAATGAGGCTCTTAAAAAATTGGAAGAATTGCTCGATAAAGACCAGTTTGAGAAAATTAAAGCAATTAACAATCC
CTACCTACACTCATTTTTGGCTGAGTGGATACAGTGGCTAGAGCCAAGTAAGGTTTACGTTTGTACGGATAGCGAAGAAG
ATGAAGAATACGTTAGATGGAAGGCCTTGTATTATGGAGAAGAGAGAATGCTCGAAATGGTGAGGCATACTGTCCACTAC
GACAACTATTATGATCAAGCTAGGGATAAACAAAATACAAAGCTTCTGGTTCCAAAAGGTACAAATTTGCCTTTCTTAAA
TACGATGGATAGGGAAGATGGGCTTAGGGAAATTAGAGAGATAATGAAAGGTATAATGAGAGGAAAGGAGCTCTTTATAG
GATTCTTTGTTCTAGGCCCCAAAAACTCAGTTTTTACTATTCCAGCTGTTCAGCTTACTGATTCTGCATATGTCATGCAC
TCAGAATTCCTCCTCTATAGAAAGGGGTATGAAGAATTCAAGAGGCTTGGTCCAACTAAGAACTTCCTAAAGTTTGTTCA
CTCTGCTGGCGAGCTGGATGAAAGGAAGACGAGCAAAAACCTTGACAAGAGGAGAATCTACATCGACTTAGTTGATGAGA
CAGTTTACTCAGCAAACACTCAATATGGTGGAAATGTCATTGGCCTAAAAAAGTTGGCCTTCAGGCTAACCATCCAAAGG
GCAGTTCGTGAGGGATGGCTAAGCGAGCACATGTTCCTCATGAGAGTTAATGGCCCGAATGGAAGGAAAACTTACTTCAC
TGGAGCCTATCCCAGCATGTGTGGAAAGACCTCAACGGCAATGATTCCCTGGGAGAACATAGTTGGCGATGACCTAGTAT
TCATAAAGAACTTGGATGGAGTTGCTAGGGCTGTAAACGTTGAGATTGGGGTATTCGGAATAATAGAGGGAATAAACCAG
AAGGATGATCCAATAATATGGCAAGTTCTACACTCCCCAGTTGAGATAATATTCTCTAACGTCCTCGTTAAGGATGGGAA
GCCGTACTGGAATGGCATGGGGATAGAGATTCCTGATGAGGGAGAGAACCACAGCGGAAAGTGGTGGAGAGGAAAGAGAG
ACGCTGAAGGTAAGGAAATACCCCCAAGCCACAAGAATGCTCGCTTTACCGTAAGGCTTGAGGCTTTCCCCAACTTGGAT
AGGGAGGCATTGGAAAATCCATGTGGAGTTGAAGTTGGTGGGATGATATTTGGTGGAAGGGATCCAGATACATGGCCGCC
GGTGAGGGAATCTTTCAATTGGGACCATGGGGTTATAACAATGGGTGCAGCTCTGGAGAGTGAAACAACCGCAGCAACCC
TGGGTAAAGAAGGTGTAAGGGCTTTCAACCCAATGTCAATCCTTGACTTTCTCAGCGTTCACATAGGAGATTACTTGAGG
AACTATTTGGAGTTTGGAAGGAAGCTCAAGAAGACACCAAAAATATTTGCAGTGAACTACTTCCTAAGGGAGAATGGCCA
ATGGCTCAATGAAAAGTTGGACAAGGCTGTGTGGCTTAAGTGGATGGAGCTGAGGGTTCACAACGATGTTGGGGCTATTG
AAACTCCTATAGGTTATATACCCAGGTACGAGGATTTAAAGGTGCTGTTTAGGCAGGTTCTCAACAAAGATTATTCAAGG
GAGGACTATGAAAAGCAGTTCAAGATAAGAGTCCCCGAGCTATTGGCTAAGATAGACAGGATATGGAAGATATATGAGCC
CATTGACAATATTCCCGAAGAACTGTTCCAGGAGCTGGAAAAGGAAAGGAAGAGGATTTTGGAGGCCAGAGAAAAATATG
GTGATTACATAAGCCCGTTTGCATTAGAAAAATAA

Upstream 100 bases:

>100_bases
TTTAAACCTTTTGGGACAATTTTGTGTATTACGGGAATTTGAATATCTGACAACAAAATTAATAATGTTAAGATGCACAC
ACTTAAACGGGTGTATAAAA

Downstream 100 bases:

>100_bases
AGGTTCAGAAGAAGAGAATTGTTGCATCTCTAACAACGGCAGTTTCAACCTCTTCGCCCTCGCTGAATACTGCCTTCTTT
ATCTCTATGTCTTCTTTTTC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MMNEALKKLEELLDKDQFEKIKAINNPYLHSFLAEWIQWLEPSKVYVCTDSEEDEEYVRWKALYYGEERMLEMVRHTVHY
DNYYDQARDKQNTKLLVPKGTNLPFLNTMDREDGLREIREIMKGIMRGKELFIGFFVLGPKNSVFTIPAVQLTDSAYVMH
SEFLLYRKGYEEFKRLGPTKNFLKFVHSAGELDERKTSKNLDKRRIYIDLVDETVYSANTQYGGNVIGLKKLAFRLTIQR
AVREGWLSEHMFLMRVNGPNGRKTYFTGAYPSMCGKTSTAMIPWENIVGDDLVFIKNLDGVARAVNVEIGVFGIIEGINQ
KDDPIIWQVLHSPVEIIFSNVLVKDGKPYWNGMGIEIPDEGENHSGKWWRGKRDAEGKEIPPSHKNARFTVRLEAFPNLD
REALENPCGVEVGGMIFGGRDPDTWPPVRESFNWDHGVITMGAALESETTAATLGKEGVRAFNPMSILDFLSVHIGDYLR
NYLEFGRKLKKTPKIFAVNYFLRENGQWLNEKLDKAVWLKWMELRVHNDVGAIETPIGYIPRYEDLKVLFRQVLNKDYSR
EDYEKQFKIRVPELLAKIDRIWKIYEPIDNIPEELFQELEKERKRILEAREKYGDYISPFALEK

Sequences:

>Translated_624_residues
MMNEALKKLEELLDKDQFEKIKAINNPYLHSFLAEWIQWLEPSKVYVCTDSEEDEEYVRWKALYYGEERMLEMVRHTVHY
DNYYDQARDKQNTKLLVPKGTNLPFLNTMDREDGLREIREIMKGIMRGKELFIGFFVLGPKNSVFTIPAVQLTDSAYVMH
SEFLLYRKGYEEFKRLGPTKNFLKFVHSAGELDERKTSKNLDKRRIYIDLVDETVYSANTQYGGNVIGLKKLAFRLTIQR
AVREGWLSEHMFLMRVNGPNGRKTYFTGAYPSMCGKTSTAMIPWENIVGDDLVFIKNLDGVARAVNVEIGVFGIIEGINQ
KDDPIIWQVLHSPVEIIFSNVLVKDGKPYWNGMGIEIPDEGENHSGKWWRGKRDAEGKEIPPSHKNARFTVRLEAFPNLD
REALENPCGVEVGGMIFGGRDPDTWPPVRESFNWDHGVITMGAALESETTAATLGKEGVRAFNPMSILDFLSVHIGDYLR
NYLEFGRKLKKTPKIFAVNYFLRENGQWLNEKLDKAVWLKWMELRVHNDVGAIETPIGYIPRYEDLKVLFRQVLNKDYSR
EDYEKQFKIRVPELLAKIDRIWKIYEPIDNIPEELFQELEKERKRILEAREKYGDYISPFALEK
>Mature_624_residues
MMNEALKKLEELLDKDQFEKIKAINNPYLHSFLAEWIQWLEPSKVYVCTDSEEDEEYVRWKALYYGEERMLEMVRHTVHY
DNYYDQARDKQNTKLLVPKGTNLPFLNTMDREDGLREIREIMKGIMRGKELFIGFFVLGPKNSVFTIPAVQLTDSAYVMH
SEFLLYRKGYEEFKRLGPTKNFLKFVHSAGELDERKTSKNLDKRRIYIDLVDETVYSANTQYGGNVIGLKKLAFRLTIQR
AVREGWLSEHMFLMRVNGPNGRKTYFTGAYPSMCGKTSTAMIPWENIVGDDLVFIKNLDGVARAVNVEIGVFGIIEGINQ
KDDPIIWQVLHSPVEIIFSNVLVKDGKPYWNGMGIEIPDEGENHSGKWWRGKRDAEGKEIPPSHKNARFTVRLEAFPNLD
REALENPCGVEVGGMIFGGRDPDTWPPVRESFNWDHGVITMGAALESETTAATLGKEGVRAFNPMSILDFLSVHIGDYLR
NYLEFGRKLKKTPKIFAVNYFLRENGQWLNEKLDKAVWLKWMELRVHNDVGAIETPIGYIPRYEDLKVLFRQVLNKDYSR
EDYEKQFKIRVPELLAKIDRIWKIYEPIDNIPEELFQELEKERKRILEAREKYGDYISPFALEK

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI66346721, Length=617, Percent_Identity=30.1458670988655, Blast_Score=243, Evalue=5e-64,
Organism=Homo sapiens, GI187281517, Length=555, Percent_Identity=31.3513513513514, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI66346723, Length=389, Percent_Identity=29.8200514138817, Blast_Score=148, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17555492, Length=634, Percent_Identity=29.4952681388013, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI32564411, Length=573, Percent_Identity=29.8429319371728, Blast_Score=217, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI32564407, Length=556, Percent_Identity=30.5755395683453, Blast_Score=214, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI71989658, Length=617, Percent_Identity=28.2009724473258, Blast_Score=209, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI71989645, Length=617, Percent_Identity=28.2009724473258, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI71989653, Length=606, Percent_Identity=28.2178217821782, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI71989649, Length=495, Percent_Identity=29.0909090909091, Blast_Score=187, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI32564409, Length=383, Percent_Identity=31.8537859007833, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24655085, Length=617, Percent_Identity=28.2009724473258, Blast_Score=232, Evalue=7e-61,
Organism=Drosophila melanogaster, GI24655088, Length=517, Percent_Identity=29.9806576402321, Blast_Score=223, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24655082, Length=559, Percent_Identity=29.8747763864043, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI161077226, Length=483, Percent_Identity=31.8840579710145, Blast_Score=213, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_PYRFU (Q8U410)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578018.1
- ProteinModelPortal:   Q8U410
- EnsemblBacteria:   EBPYRT00000004205
- GeneID:   1468123
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0289
- NMPDR:   fig|186497.1.peg.294
- GeneTree:   EBGT00050000022626
- HOGENOM:   HBG484437
- OMA:   QEKRVQS
- ProtClustDB:   PRK04210
- BRENDA:   4.1.1.32
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 72666; Mature: 72666

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 274-274 BINDING 88-88 BINDING 224-224 BINDING 231-231 BINDING 272-272 BINDING 388-388 BINDING 420-420

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNEALKKLEELLDKDQFEKIKAINNPYLHSFLAEWIQWLEPSKVYVCTDSEEDEEYVRW
CCHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
KALYYGEERMLEMVRHTVHYDNYYDQARDKQNTKLLVPKGTNLPFLNTMDREDGLREIRE
HHHHCCHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
IMKGIMRGKELFIGFFVLGPKNSVFTIPAVQLTDSAYVMHSEFLLYRKGYEEFKRLGPTK
HHHHHHCCCEEEEEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCHH
NFLKFVHSAGELDERKTSKNLDKRRIYIDLVDETVYSANTQYGGNVIGLKKLAFRLTIQR
HHHHHHHHHCCHHHHHHHCCCCHHEEEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
AVREGWLSEHMFLMRVNGPNGRKTYFTGAYPSMCGKTSTAMIPWENIVGDDLVFIKNLDG
HHHHCHHCCCEEEEEEECCCCCEEEECCCCHHHCCCCCCEECCHHHHCCCCEEEEECCCH
VARAVNVEIGVFGIIEGINQKDDPIIWQVLHSPVEIIFSNVLVKDGKPYWNGMGIEIPDE
HHHEEEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCC
GENHSGKWWRGKRDAEGKEIPPSHKNARFTVRLEAFPNLDREALENPCGVEVGGMIFGGR
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCEECCEEECCC
DPDTWPPVRESFNWDHGVITMGAALESETTAATLGKEGVRAFNPMSILDFLSVHIGDYLR
CCCCCCCHHHCCCCCCCEEEECCHHCCCHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHH
NYLEFGRKLKKTPKIFAVNYFLRENGQWLNEKLDKAVWLKWMELRVHNDVGAIETPIGYI
HHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCC
PRYEDLKVLFRQVLNKDYSREDYEKQFKIRVPELLAKIDRIWKIYEPIDNIPEELFQELE
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KERKRILEAREKYGDYISPFALEK
HHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MMNEALKKLEELLDKDQFEKIKAINNPYLHSFLAEWIQWLEPSKVYVCTDSEEDEEYVRW
CCHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
KALYYGEERMLEMVRHTVHYDNYYDQARDKQNTKLLVPKGTNLPFLNTMDREDGLREIRE
HHHHCCHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
IMKGIMRGKELFIGFFVLGPKNSVFTIPAVQLTDSAYVMHSEFLLYRKGYEEFKRLGPTK
HHHHHHCCCEEEEEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCHH
NFLKFVHSAGELDERKTSKNLDKRRIYIDLVDETVYSANTQYGGNVIGLKKLAFRLTIQR
HHHHHHHHHCCHHHHHHHCCCCHHEEEEEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
AVREGWLSEHMFLMRVNGPNGRKTYFTGAYPSMCGKTSTAMIPWENIVGDDLVFIKNLDG
HHHHCHHCCCEEEEEEECCCCCEEEECCCCHHHCCCCCCEECCHHHHCCCCEEEEECCCH
VARAVNVEIGVFGIIEGINQKDDPIIWQVLHSPVEIIFSNVLVKDGKPYWNGMGIEIPDE
HHHEEEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCC
GENHSGKWWRGKRDAEGKEIPPSHKNARFTVRLEAFPNLDREALENPCGVEVGGMIFGGR
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCEECCEEECCC
DPDTWPPVRESFNWDHGVITMGAALESETTAATLGKEGVRAFNPMSILDFLSVHIGDYLR
CCCCCCCHHHCCCCCCCEEEECCHHCCCHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHH
NYLEFGRKLKKTPKIFAVNYFLRENGQWLNEKLDKAVWLKWMELRVHNDVGAIETPIGYI
HHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCC
PRYEDLKVLFRQVLNKDYSREDYEKQFKIRVPELLAKIDRIWKIYEPIDNIPEELFQELE
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KERKRILEAREKYGDYISPFALEK
HHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA