Click here to switch to the map view.

The map label for this gene is bioB [C]

Identifier: 18976516

GI number: 18976516

Start: 153721

End: 154650

Strand: Direct

Name: bioB [C]

Synonym: PF0144

Alternate gene names: 18976516

Gene position: 153721-154650 (Clockwise)

Preceding gene: 18976515

Following gene: 18976517

Centisome position: 8.06

GC content: 40.75

Gene sequence:

>930_bases
ATGAAGGTTAGAGTTTCATATGGAACGGCTATAGCCATGGGGCTAATTAAGGCCAAGATGCTCTCTAGCCCTACTACAGC
TTATCTCATGACGTATCACAATGGGAGATGTCTTAATGATTGCAAATTTTGCCCCCAGGCTAGATCAAGTAAGGCAGATA
TAGAGAAGCTTTCCCGAATAACTTGGCCGGTTTTTGAACTTGAAGATGTTATTAAGAATTTTCATAGAGGAAATTTCTCT
AGAATATGTCTTCAGACAGTAGATTACCCAGAACTAGTTGAAGATGTTCTTAAAATCACTGAAGCTTTGAGTGAGCTAGG
CAAGCCAATCTCTATTTCAATAACTCCAGTTTCTAGAGGCACTCTGGAGGAGCTTAAAAGCAATGGTGTTGATTACATAG
GGATAGGGCTTGATGCAGGGAGTAAAGAAGTTTATGAAAAGGTAAAGATTTCGAGATACTCCTGGGATGATATGTGGAAG
TTTTTGGATGAAACAGTTAGAGTTTTTGGAAAGGGCAGGGGAGTTGTGCACCTAATAGTAGGACTTGGTGAGAAGGATAT
TGATTTGCTAAGGACCATAGAGGAGGTCTATTCTAGAGGGGGCAGTGTCTCTCTGTTTGCTTTCACTCCAATTAAGGGAA
CTGCTCTGGAGAAACTTTCCTCTCCACCAATAGAGAGATATAGGAAAATTCAGGCTGCTCACTACCTTATAAAGGCTGGA
AGGGCTAAAGTTGAAGACTTTGAGTTTGATGACGCAGGTAATTTAATTAGAATCCCACATAAAGTCCCTTATACCGCATT
TCTTACTCAAGGGTGTCCGTTCTGTAATAGGCCATATTATAACGAAAGGCCTGGAAGAGAACTATACAACTATCCTTCCA
TTGAGGTTCTTGTAGAGGATATGGAGAAAGGAAGGTTCACGGGTGTTTAA

Upstream 100 bases:

>100_bases
GCTGAAGTTAGAGGGTATGAGGGAATATTTCCCTTTATTGCTCTGCTCCCATTCGTGTCCCTTATAACTGTACATTTCCC
ATTATTAAGGAGGGAAGGAA

Downstream 100 bases:

>100_bases
AATGGAAGTAGTTCCCATAGGGTATATAAGGAAGACAGACAGGGATGTTTTCATTGAGATTCTAGATGAGTTTTTGGAGG
GACTAGAAGGGCTTGAAGAG

Product: hypothetical protein

Products: biotin

Alternate protein names: Radical SAM Domain-Containing Protein; Biotin Synthase Related Protein; Biotin Synthase; Biotin Synthase-Related Protein; Biotin Synthase-Related; Radical SAM Family Protein; Radical SAM Superfamily Protein

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKVRVSYGTAIAMGLIKAKMLSSPTTAYLMTYHNGRCLNDCKFCPQARSSKADIEKLSRITWPVFELEDVIKNFHRGNFS
RICLQTVDYPELVEDVLKITEALSELGKPISISITPVSRGTLEELKSNGVDYIGIGLDAGSKEVYEKVKISRYSWDDMWK
FLDETVRVFGKGRGVVHLIVGLGEKDIDLLRTIEEVYSRGGSVSLFAFTPIKGTALEKLSSPPIERYRKIQAAHYLIKAG
RAKVEDFEFDDAGNLIRIPHKVPYTAFLTQGCPFCNRPYYNERPGRELYNYPSIEVLVEDMEKGRFTGV

Sequences:

>Translated_309_residues
MKVRVSYGTAIAMGLIKAKMLSSPTTAYLMTYHNGRCLNDCKFCPQARSSKADIEKLSRITWPVFELEDVIKNFHRGNFS
RICLQTVDYPELVEDVLKITEALSELGKPISISITPVSRGTLEELKSNGVDYIGIGLDAGSKEVYEKVKISRYSWDDMWK
FLDETVRVFGKGRGVVHLIVGLGEKDIDLLRTIEEVYSRGGSVSLFAFTPIKGTALEKLSSPPIERYRKIQAAHYLIKAG
RAKVEDFEFDDAGNLIRIPHKVPYTAFLTQGCPFCNRPYYNERPGRELYNYPSIEVLVEDMEKGRFTGV
>Mature_309_residues
MKVRVSYGTAIAMGLIKAKMLSSPTTAYLMTYHNGRCLNDCKFCPQARSSKADIEKLSRITWPVFELEDVIKNFHRGNFS
RICLQTVDYPELVEDVLKITEALSELGKPISISITPVSRGTLEELKSNGVDYIGIGLDAGSKEVYEKVKISRYSWDDMWK
FLDETVRVFGKGRGVVHLIVGLGEKDIDLLRTIEEVYSRGGSVSLFAFTPIKGTALEKLSSPPIERYRKIQAAHYLIKAG
RAKVEDFEFDDAGNLIRIPHKVPYTAFLTQGCPFCNRPYYNERPGRELYNYPSIEVLVEDMEKGRFTGV

Specific function: Biotin biosynthesis; last step. [C]

COG id: COG2516

COG function: function code R; Biotin synthase-related enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.8.1.6

Molecular weight: Translated: 34929; Mature: 34929

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRVSYGTAIAMGLIKAKMLSSPTTAYLMTYHNGRCLNDCKFCPQARSSKADIEKLSRI
CEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHH
TWPVFELEDVIKNFHRGNFSRICLQTVDYPELVEDVLKITEALSELGKPISISITPVSRG
CCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCH
TLEELKSNGVDYIGIGLDAGSKEVYEKVKISRYSWDDMWKFLDETVRVFGKGRGVVHLIV
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
GLGEKDIDLLRTIEEVYSRGGSVSLFAFTPIKGTALEKLSSPPIERYRKIQAAHYLIKAG
ECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHC
RAKVEDFEFDDAGNLIRIPHKVPYTAFLTQGCPFCNRPYYNERPGRELYNYPSIEVLVED
CCCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHH
MEKGRFTGV
HHCCCCCCC
>Mature Secondary Structure
MKVRVSYGTAIAMGLIKAKMLSSPTTAYLMTYHNGRCLNDCKFCPQARSSKADIEKLSRI
CEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHH
TWPVFELEDVIKNFHRGNFSRICLQTVDYPELVEDVLKITEALSELGKPISISITPVSRG
CCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCH
TLEELKSNGVDYIGIGLDAGSKEVYEKVKISRYSWDDMWKFLDETVRVFGKGRGVVHLIV
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
GLGEKDIDLLRTIEEVYSRGGSVSLFAFTPIKGTALEKLSSPPIERYRKIQAAHYLIKAG
ECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHC
RAKVEDFEFDDAGNLIRIPHKVPYTAFLTQGCPFCNRPYYNERPGRELYNYPSIEVLVED
CCCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHH
MEKGRFTGV
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.002 {dethiobiotin}} [C]

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: Sulfur atom transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA