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The map label for this gene is birA [H]

Identifier: 18976449

GI number: 18976449

Start: 82448

End: 83155

Strand: Direct

Name: birA [H]

Synonym: PF0077

Alternate gene names: 18976449

Gene position: 82448-83155 (Clockwise)

Preceding gene: 18976448

Following gene: 18976452

Centisome position: 4.32

GC content: 42.8

Gene sequence:

>708_bases
ATGCTGGGCTTGAACACTTCAATTATTGGGAAGGTGGTTATATACTATAGAGAAGTCACATCCACAAATGAAGTAGCAAA
GCTCGGCAATTTTGAAGAAGGAACTGTAATAGTTGCTGACACTCAAACTATGGGACATGGAAGAATTGGTAGGAGGTGGG
AATCTCCACCTGGCGGGCTGTGGATGTCCGTGGTTTTGCATCCTAGAATTTCTACCGAGGATCTCCCGAAATTGAGCTTT
CTAGGGGCATTAGCAGTTGTTAGGACTCTCTCTGAATTTTCCATTCAGGGGAAGATAAAGTGGCCAAACGATGTTCTTGT
TGATTACAAGAAAATAGCTGGAATACTCGTTGAGAAAAGAGGGGATAACGTTGTGCTTGGAGTAGGGCTAAACGTAAACA
ATGAAGCCCCAGAGAATGCCACGTCTATGGCCGAGATATTGGGAACTTCCGTACCTTTAATTGAGGTTTTCCGAGCATTG
GTGACTAACTTAGATGAATTGTACAAGATGTTTTTGTCTTCCCCAGCAAGCCTTCTTGAGATGGCAAAAAAGGAAATGAT
TCTTGGAGTTCCCGTTAGAATTATTGGGGATGGAGAAATTGAAGGCATTGCTGAAGACATCGACGAGCTTGGAAGACTAA
TTCTAAGGCTCTCAGATGGAAGCCTAAAGAAAATTGTTTATGGAGATGTGTCACTTAGGTTCCTCTAG

Upstream 100 bases:

>100_bases
CATTTCCAGCAAAGATGGTGGTGGATTATGTAAGGGTTTACTCATTCGTTAGTGGTTGAGGTTGGGAAAGATTTTATTGC
TTTGTTGAGCATAAAATAAT

Downstream 100 bases:

>100_bases
CATGTCTAGTCCTTTCTTCTTCATTATTTCTACAGTTTTTTCCATAATGTCCTCCATGATTGTGGAGATTGTTTTTTCAA
GCTCTACATTCTCAATTATG

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MLGLNTSIIGKVVIYYREVTSTNEVAKLGNFEEGTVIVADTQTMGHGRIGRRWESPPGGLWMSVVLHPRISTEDLPKLSF
LGALAVVRTLSEFSIQGKIKWPNDVLVDYKKIAGILVEKRGDNVVLGVGLNVNNEAPENATSMAEILGTSVPLIEVFRAL
VTNLDELYKMFLSSPASLLEMAKKEMILGVPVRIIGDGEIEGIAEDIDELGRLILRLSDGSLKKIVYGDVSLRFL

Sequences:

>Translated_235_residues
MLGLNTSIIGKVVIYYREVTSTNEVAKLGNFEEGTVIVADTQTMGHGRIGRRWESPPGGLWMSVVLHPRISTEDLPKLSF
LGALAVVRTLSEFSIQGKIKWPNDVLVDYKKIAGILVEKRGDNVVLGVGLNVNNEAPENATSMAEILGTSVPLIEVFRAL
VTNLDELYKMFLSSPASLLEMAKKEMILGVPVRIIGDGEIEGIAEDIDELGRLILRLSDGSLKKIVYGDVSLRFL
>Mature_235_residues
MLGLNTSIIGKVVIYYREVTSTNEVAKLGNFEEGTVIVADTQTMGHGRIGRRWESPPGGLWMSVVLHPRISTEDLPKLSF
LGALAVVRTLSEFSIQGKIKWPNDVLVDYKKIAGILVEKRGDNVVLGVGLNVNNEAPENATSMAEILGTSVPLIEVFRAL
VTNLDELYKMFLSSPASLLEMAKKEMILGVPVRIIGDGEIEGIAEDIDELGRLILRLSDGSLKKIVYGDVSLRFL

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=232, Percent_Identity=30.1724137931034, Blast_Score=78, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24644246, Length=146, Percent_Identity=32.1917808219178, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 25707; Mature: 25707

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLNTSIIGKVVIYYREVTSTNEVAKLGNFEEGTVIVADTQTMGHGRIGRRWESPPGGL
CCCCCHHHHHHHHEEEEHHCCCHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCE
WMSVVLHPRISTEDLPKLSFLGALAVVRTLSEFSIQGKIKWPNDVLVDYKKIAGILVEKR
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHEEEEEC
GDNVVLGVGLNVNNEAPENATSMAEILGTSVPLIEVFRALVTNLDELYKMFLSSPASLLE
CCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHCCHHHHHH
MAKKEMILGVPVRIIGDGEIEGIAEDIDELGRLILRLSDGSLKKIVYGDVSLRFL
HHHHHHEECCCEEEEECCCHHHHHHHHHHHHHHHEEECCCCEEEEEEECEEEEEC
>Mature Secondary Structure
MLGLNTSIIGKVVIYYREVTSTNEVAKLGNFEEGTVIVADTQTMGHGRIGRRWESPPGGL
CCCCCHHHHHHHHEEEEHHCCCHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCE
WMSVVLHPRISTEDLPKLSFLGALAVVRTLSEFSIQGKIKWPNDVLVDYKKIAGILVEKR
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHEEEEEC
GDNVVLGVGLNVNNEAPENATSMAEILGTSVPLIEVFRALVTNLDELYKMFLSSPASLLE
CCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHCCHHHHHH
MAKKEMILGVPVRIIGDGEIEGIAEDIDELGRLILRLSDGSLKKIVYGDVSLRFL
HHHHHHEECCCEEEEECCCHHHHHHHHHHHHHHHEEECCCCEEEEEEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]