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The map label for this gene is bdhA [H]

Identifier: 18976447

GI number: 18976447

Start: 80328

End: 81500

Strand: Direct

Name: bdhA [H]

Synonym: PF0075

Alternate gene names: 18976447

Gene position: 80328-81500 (Clockwise)

Preceding gene: 18976446

Following gene: 18976448

Centisome position: 4.21

GC content: 43.14

Gene sequence:

>1173_bases
ATGTTTGAGATATCAATTTATCTTCCCACAGAAATAGTTTTTGGTCCTGGGAAGCTTGAAATGCTTCCTAAACTAGTGAA
GAAGCATGGGCTTTCTGGGAAGGCCCTAATAGTAACTGGAAGGAGAAGCACAAAGGAAACTGGAGTTCTTTATAGAGTTC
AAGAACTACTTAAGCAAGCTGGGGTAGAGAGCATAGTTTTTGACAAAATTATTCCAAATCCAATATCTACTCATGTGGAT
GAAGGGGCAGAGATAGCGAGAAAAGAAAATGTTAGCTTTGTTGTTGGCTTGGGTGGTGGAAGTGCGATAGATAGTGCAAA
AGCTATAGCAATGACTGCCGCCAGTGGAGGTAAATATTGGGACTATGTTCCAGCTGTGGGAGGAGGAAAGAAGCCTACTG
GAGCGCTTCCAATAGTTGCAATTCCAACAACCCACGGGACTGGAACGGAGGCTGATCCTTATGCTGTTATAACTAATCCT
GAAACAAAGGAGAAGCAGGGAATTGGATATGATGTTCTCTTCCCCAAATTCTCTATAGTTGATCCAGAACTTATGCTTAC
TCTTCCAAAAGATCAAACAGTGTACACTTCAATGGATGCTTTCTACCACTCCATTGAGGCCTTTCTTAATGTTAGAGCAA
ATCCATATTCGGATGTTCTGGCTCTCGACTCAATGAGGCGCATTGTTACATACCTTCCATTGGCCTACGAAAACTTGAGA
AATCTTGAAGCAAGAACGCAACTTGCCTGGGCAAGTACTGAGGCTGGAATAACGGAAACGGTAACGGGAGTTGTGGCAAA
TCATGCACTTGAGCATGGTCTAAGTGGATTCTATCCTGAAGTGCCTCATGGTCTGGGCCTCTGCATTCTAGGACCCTACC
TCTTTGAATACATTCTCGACTATGCCTATGAAAAGTTGGCGATAGTCGGAAGAGAGGTATTTGGAGTTTACGAGCCAAAT
GACAGAAAGGCAGCAGAGCTAGCTATTAAGAAGCTACGTGACTTCCAGAGCCTCTTTGGAGTAAACAAGAAGCTCAGAGA
ATTAGGGGTTAAAGAGGAAGACATTCCAGAGATGGCTAGGACTGCTTATAGAATGATGAAACCTGTTATAGAGGCAACAC
CGGGAGATTTGAAAGTTGAAGACTTGGAAGAGATCTATAGAAGAGCATACTAA

Upstream 100 bases:

>100_bases
GAGGCAGTAAAATACCTCCTAAGACTTCCAAAGGATGTTAGGGTTGAGGAATTAATGTTGCGCTCAATTTATCAAAAACC
TGAGTATTGAGGTGGTGTGC

Downstream 100 bases:

>100_bases
CGGGCTGACACAATGAAAAAAGAAGCCTTGTTGTTTCTTTCCTTAATTTTTCTCGTGTTTGTAAGTGGATGTATTCATCA
TTCAACTAATCAACAACTTT

Product: alcohol dehydrogenase

Products: Glycerol; Nicotinamide adenine dinucleotide [C]

Alternate protein names: BDH I [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MFEISIYLPTEIVFGPGKLEMLPKLVKKHGLSGKALIVTGRRSTKETGVLYRVQELLKQAGVESIVFDKIIPNPISTHVD
EGAEIARKENVSFVVGLGGGSAIDSAKAIAMTAASGGKYWDYVPAVGGGKKPTGALPIVAIPTTHGTGTEADPYAVITNP
ETKEKQGIGYDVLFPKFSIVDPELMLTLPKDQTVYTSMDAFYHSIEAFLNVRANPYSDVLALDSMRRIVTYLPLAYENLR
NLEARTQLAWASTEAGITETVTGVVANHALEHGLSGFYPEVPHGLGLCILGPYLFEYILDYAYEKLAIVGREVFGVYEPN
DRKAAELAIKKLRDFQSLFGVNKKLRELGVKEEDIPEMARTAYRMMKPVIEATPGDLKVEDLEEIYRRAY

Sequences:

>Translated_390_residues
MFEISIYLPTEIVFGPGKLEMLPKLVKKHGLSGKALIVTGRRSTKETGVLYRVQELLKQAGVESIVFDKIIPNPISTHVD
EGAEIARKENVSFVVGLGGGSAIDSAKAIAMTAASGGKYWDYVPAVGGGKKPTGALPIVAIPTTHGTGTEADPYAVITNP
ETKEKQGIGYDVLFPKFSIVDPELMLTLPKDQTVYTSMDAFYHSIEAFLNVRANPYSDVLALDSMRRIVTYLPLAYENLR
NLEARTQLAWASTEAGITETVTGVVANHALEHGLSGFYPEVPHGLGLCILGPYLFEYILDYAYEKLAIVGREVFGVYEPN
DRKAAELAIKKLRDFQSLFGVNKKLRELGVKEEDIPEMARTAYRMMKPVIEATPGDLKVEDLEEIYRRAY
>Mature_390_residues
MFEISIYLPTEIVFGPGKLEMLPKLVKKHGLSGKALIVTGRRSTKETGVLYRVQELLKQAGVESIVFDKIIPNPISTHVD
EGAEIARKENVSFVVGLGGGSAIDSAKAIAMTAASGGKYWDYVPAVGGGKKPTGALPIVAIPTTHGTGTEADPYAVITNP
ETKEKQGIGYDVLFPKFSIVDPELMLTLPKDQTVYTSMDAFYHSIEAFLNVRANPYSDVLALDSMRRIVTYLPLAYENLR
NLEARTQLAWASTEAGITETVTGVVANHALEHGLSGFYPEVPHGLGLCILGPYLFEYILDYAYEKLAIVGREVFGVYEPN
DRKAAELAIKKLRDFQSLFGVNKKLRELGVKEEDIPEMARTAYRMMKPVIEATPGDLKVEDLEEIYRRAY

Specific function: Unknown

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=387, Percent_Identity=26.6149870801034, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI48994951, Length=397, Percent_Identity=33.7531486146096, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789163, Length=382, Percent_Identity=29.5811518324607, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787493, Length=378, Percent_Identity=29.3650793650794, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789386, Length=364, Percent_Identity=28.5714285714286, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87082107, Length=385, Percent_Identity=25.974025974026, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17537053, Length=393, Percent_Identity=26.972010178117, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321181, Length=386, Percent_Identity=33.9378238341969, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24657991, Length=426, Percent_Identity=27.6995305164319, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1 [C]

Molecular weight: Translated: 42909; Mature: 42909

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEISIYLPTEIVFGPGKLEMLPKLVKKHGLSGKALIVTGRRSTKETGVLYRVQELLKQA
CEEEEEEECCEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
GVESIVFDKIIPNPISTHVDEGAEIARKENVSFVVGLGGGSAIDSAKAIAMTAASGGKYW
CCHHHHHHHHCCCCHHHHHCCCHHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCCE
DYVPAVGGGKKPTGALPIVAIPTTHGTGTEADPYAVITNPETKEKQGIGYDVLFPKFSIV
EECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCHHCCCCEEEECCCCEEC
DPELMLTLPKDQTVYTSMDAFYHSIEAFLNVRANPYSDVLALDSMRRIVTYLPLAYENLR
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NLEARTQLAWASTEAGITETVTGVVANHALEHGLSGFYPEVPHGLGLCILGPYLFEYILD
HHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YAYEKLAIVGREVFGVYEPNDRKAAELAIKKLRDFQSLFGVNKKLRELGVKEEDIPEMAR
HHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHH
TAYRMMKPVIEATPGDLKVEDLEEIYRRAY
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MFEISIYLPTEIVFGPGKLEMLPKLVKKHGLSGKALIVTGRRSTKETGVLYRVQELLKQA
CEEEEEEECCEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
GVESIVFDKIIPNPISTHVDEGAEIARKENVSFVVGLGGGSAIDSAKAIAMTAASGGKYW
CCHHHHHHHHCCCCHHHHHCCCHHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCCE
DYVPAVGGGKKPTGALPIVAIPTTHGTGTEADPYAVITNPETKEKQGIGYDVLFPKFSIV
EECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCHHCCCCEEEECCCCEEC
DPELMLTLPKDQTVYTSMDAFYHSIEAFLNVRANPYSDVLALDSMRRIVTYLPLAYENLR
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NLEARTQLAWASTEAGITETVTGVVANHALEHGLSGFYPEVPHGLGLCILGPYLFEYILD
HHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YAYEKLAIVGREVFGVYEPNDRKAAELAIKKLRDFQSLFGVNKKLRELGVKEEDIPEMAR
HHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHH
TAYRMMKPVIEATPGDLKVEDLEEIYRRAY
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.004 {benzyl} 0.005 {cyclohe

Substrates: D-Glyceraldehyde; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: D-Glyceraldehyde + H+ + Nicotinamide adenine dinucleotide - reduced <==> Glycerol + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1385386; 11466286; 1809209 [H]