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The map label for this gene is cobB

Identifier: 161484709

GI number: 161484709

Start: 1103228

End: 1103980

Strand: Reverse

Name: cobB

Synonym: PF1154

Alternate gene names: 161484709

Gene position: 1103980-1103228 (Counterclockwise)

Preceding gene: 18977528

Following gene: 18977525

Centisome position: 57.85

GC content: 44.22

Gene sequence:

>753_bases
ATGCTAGGTGAAGTGAGCAAAATCTTAGCAAAATCTTCTATGGCAATAGCATTTACTGGAGCGGGCATTAGTGCTGAGAG
TGGTATCCCCACTTTTAGGGGAAAAGATGGACTATGGAGAAAGTATAGGGCTGAGGAGCTAGCTACTCCTGAAGCTTTTA
AGAGAGATCCAAAGCTTGTATGGGAGTTCTACAAGTGGAGAATCAAGAAGATTTTGGAAGCGAAGCCAAATCCTGCTCAC
ATAGCTTTGGCAGAGCTGGAGAAGATGGGAATAATAAAGGCCGTGATTACCCAAAACGTTGATGATCTTCACAGGGAAGC
AGGGAGCAAGAATGTTATAGAGTTGCACGGAAATATATTTCGAGTTAAATGCACGAGTTGCTCATATAGAGAATACTTGA
AGGAAAGTGACAGAATTGGGTGGCTACTTTCCCAAGAACTACCTAGGTGTCCCAAGTGTGGCTCTCTTCTAAGGCCCGAT
GTAGTTTGGTTTGGCGAGGCTTTGCCCGAAAAAGAGCTAACAACAGCGTTTTCACTAGCTAAAAAAGCTGATGTTGTTCT
CGTTGTAGGAACTAGTGGCGTCGTATACCCTGCCGCATACATACCATATATAGTTAAGGAGAGCGGGGGTATTGTCGTTG
AGATAAACATTGAACCCTCTGCAATCACTCCGATAGCGGACTTCTTCCTTAGGGGCAAAGCTGGAGAAGTTTTGCCAAAG
TTGGTTGAGGAGATCAGGAGGATTTCTAAATGA

Upstream 100 bases:

>100_bases
GGTGCAAGGCAATTACATGTTGCATGTTCTATCATTAAAGGGTGAATATAAAAATATTAGCCCAAAAGAAATTTTAAGGT
ATCCTTCTACTAATTCTCTG

Downstream 100 bases:

>100_bases
GCAGAATGCCCCTCTATTTAAAACATCGAGTTGAGAAGGTAAAGGAAAGGTATCTCTATGAAATTTATGAGAGCAAAAAA
CTTCCCCCCTGGGAGAGATT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MLGEVSKILAKSSMAIAFTGAGISAESGIPTFRGKDGLWRKYRAEELATPEAFKRDPKLVWEFYKWRIKKILEAKPNPAH
IALAELEKMGIIKAVITQNVDDLHREAGSKNVIELHGNIFRVKCTSCSYREYLKESDRIGWLLSQELPRCPKCGSLLRPD
VVWFGEALPEKELTTAFSLAKKADVVLVVGTSGVVYPAAYIPYIVKESGGIVVEINIEPSAITPIADFFLRGKAGEVLPK
LVEEIRRISK

Sequences:

>Translated_250_residues
MLGEVSKILAKSSMAIAFTGAGISAESGIPTFRGKDGLWRKYRAEELATPEAFKRDPKLVWEFYKWRIKKILEAKPNPAH
IALAELEKMGIIKAVITQNVDDLHREAGSKNVIELHGNIFRVKCTSCSYREYLKESDRIGWLLSQELPRCPKCGSLLRPD
VVWFGEALPEKELTTAFSLAKKADVVLVVGTSGVVYPAAYIPYIVKESGGIVVEINIEPSAITPIADFFLRGKAGEVLPK
LVEEIRRISK
>Mature_250_residues
MLGEVSKILAKSSMAIAFTGAGISAESGIPTFRGKDGLWRKYRAEELATPEAFKRDPKLVWEFYKWRIKKILEAKPNPAH
IALAELEKMGIIKAVITQNVDDLHREAGSKNVIELHGNIFRVKCTSCSYREYLKESDRIGWLLSQELPRCPKCGSLLRPD
VVWFGEALPEKELTTAFSLAKKADVVLVVGTSGVVYPAAYIPYIVKESGGIVVEINIEPSAITPIADFFLRGKAGEVLPK
LVEEIRRISK

Specific function: Modulates the activity of chromatin proteins, thereby having an effect on transcription per subunit

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain

Homologues:

Organism=Homo sapiens, GI6912664, Length=260, Percent_Identity=41.5384615384615, Blast_Score=196, Evalue=1e-50,
Organism=Homo sapiens, GI300795542, Length=243, Percent_Identity=44.0329218106996, Blast_Score=194, Evalue=8e-50,
Organism=Homo sapiens, GI13787215, Length=249, Percent_Identity=42.1686746987952, Blast_Score=188, Evalue=3e-48,
Organism=Homo sapiens, GI300797577, Length=252, Percent_Identity=30.1587301587302, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI6912662, Length=266, Percent_Identity=32.7067669172932, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI7657575, Length=226, Percent_Identity=31.4159292035398, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI63054862, Length=198, Percent_Identity=34.8484848484849, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI300797597, Length=246, Percent_Identity=28.4552845528455, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI6912660, Length=220, Percent_Identity=33.1818181818182, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI13775602, Length=199, Percent_Identity=34.6733668341709, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI13775600, Length=199, Percent_Identity=34.6733668341709, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI300797705, Length=190, Percent_Identity=34.7368421052632, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI7706712, Length=254, Percent_Identity=27.5590551181102, Blast_Score=90, Evalue=1e-18,
Organism=Homo sapiens, GI215982798, Length=174, Percent_Identity=31.0344827586207, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI308199517, Length=234, Percent_Identity=42.3076923076923, Blast_Score=177, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17567771, Length=272, Percent_Identity=31.6176470588235, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71990482, Length=274, Percent_Identity=30.2919708029197, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17541892, Length=220, Percent_Identity=29.0909090909091, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71990487, Length=276, Percent_Identity=30.0724637681159, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17505510, Length=235, Percent_Identity=27.2340425531915, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325242, Length=219, Percent_Identity=31.5068493150685, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324599, Length=280, Percent_Identity=26.4285714285714, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320163, Length=233, Percent_Identity=25.7510729613734, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320397, Length=261, Percent_Identity=25.6704980842912, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571445, Length=266, Percent_Identity=31.5789473684211, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI17137536, Length=236, Percent_Identity=31.3559322033898, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28571443, Length=207, Percent_Identity=30.9178743961353, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28571441, Length=207, Percent_Identity=30.9178743961353, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24645650, Length=252, Percent_Identity=28.968253968254, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648389, Length=203, Percent_Identity=31.5270935960591, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24650933, Length=253, Percent_Identity=23.7154150197628, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPD_PYRFU (Q8U1Q1)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578883.2
- ProteinModelPortal:   Q8U1Q1
- EnsemblBacteria:   EBPYRT00000005189
- GeneID:   1469023
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1154
- NMPDR:   fig|186497.1.peg.1194
- GeneTree:   EBGT00050000023311
- HOGENOM:   HBG641281
- OMA:   FVHEARL
- ProtClustDB:   PRK00481
- GO:   GO:0005737
- GO:   GO:0006350
- HAMAP:   MF_01121
- InterPro:   IPR003000
- PANTHER:   PTHR11085

Pfam domain/function: PF02146 SIR2

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27786; Mature: 27786

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50305 SIRTUIN

Important sites: ACT_SITE 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGEVSKILAKSSMAIAFTGAGISAESGIPTFRGKDGLWRKYRAEELATPEAFKRDPKLV
CCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHH
WEFYKWRIKKILEAKPNPAHIALAELEKMGIIKAVITQNVDDLHREAGSKNVIELHGNIF
HHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEECCCEE
RVKCTSCSYREYLKESDRIGWLLSQELPRCPKCGSLLRPDVVWFGEALPEKELTTAFSLA
EEEECCCHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHH
KKADVVLVVGTSGVVYPAAYIPYIVKESGGIVVEINIEPSAITPIADFFLRGKAGEVLPK
HHCCEEEEECCCCCEEHHHHHCEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHH
LVEEIRRISK
HHHHHHHHCC
>Mature Secondary Structure
MLGEVSKILAKSSMAIAFTGAGISAESGIPTFRGKDGLWRKYRAEELATPEAFKRDPKLV
CCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHH
WEFYKWRIKKILEAKPNPAHIALAELEKMGIIKAVITQNVDDLHREAGSKNVIELHGNIF
HHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEECCCEE
RVKCTSCSYREYLKESDRIGWLLSQELPRCPKCGSLLRPDVVWFGEALPEKELTTAFSLA
EEEECCCHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHH
KKADVVLVVGTSGVVYPAAYIPYIVKESGGIVVEINIEPSAITPIADFFLRGKAGEVLPK
HHCCEEEEECCCCCEEHHHHHCEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHH
LVEEIRRISK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA