Click here to switch to the map view.

The map label for this gene is leuA2 [H]

Identifier: 161484707

GI number: 161484707

Start: 1560860

End: 1561858

Strand: Direct

Name: leuA2 [H]

Synonym: PF1678

Alternate gene names: 161484707

Gene position: 1560860-1561858 (Clockwise)

Preceding gene: 18978049

Following gene: 18978051

Centisome position: 81.8

GC content: 40.14

Gene sequence:

>999_bases
TTGGACGAGTTTGGCGTTGAGTTCATTGAAGTTGGGCATCCCGCAGTGAGCAGAGATGTATTCGAGGGGATAAAACTTGT
AGTTCAGCAGGGACTAAAGGCAAATCTTCTTGCACATTCAAGGGCCTTAATAAGTGATGTTGACTATGTTTTGAAGGCAG
GGACAGACTGGATAGGAATATTCTTCTGCCTATCAAACCACTGCCTAACCAAGAGGTTTGGAATAACACTAGAGCAAGCA
TTAGATAGAATCAGTAGAGCTATAGAATATGCAAAAGACCATGGATTAAAAGTTAGATTTACTCCAGAAGACACAACGAG
AACTGAGTGGAAGAACTTAGTAGCCGCGCTTAATCTTGCAAAGGAACTAAAAGTTGATAGAGTTAGCATAGCAGATACAA
CTGGAAGTGCTCACCCCTTAAAATTCTACATGCTCGTTAAAAACGTCACAGAGTTTGGAATACCTGTAAACGTCCACTGT
CACAACGACCTTGGCTTAGCTCTTGCAAATGCAATTATGGGAATCGAGGCAGGTGCAACACTGGTGGATGCTACAATAAA
TGGATTAGGGGAAAGAGCAGGAATTGTTGATTTGGCCCAAATTGCTACAGTTCTATACTACCATTATGGCATCAAAAAGT
ATAAGCTAGAGATGCTTTACTACTTAAGCCAGCTACTTACCAAGATAACAGGAATAAGACCACAGCAAAACTACCCAATA
GTGGGGGAGAACGCATTTACACATAAAGCTGGACTTCATGTCTCTGCTGTAATAAAAGACCCAACTTTTTATGAATTTCT
GCCAGCAGAAACCTTCGGCAGGGAAAGAAGGATCTATCTAGACAGATTTGCTGGTAGGGACACAATTAGATTTTATTTGA
GAAAAGCAGGTATTGAAAACGAAAATATTGTTGAAGAACTTCTAAGAAGAGTAAAAGAAAGCAGAGAACCTTATACCTGG
GAAAAATTAATAGAAGAAGCTAGGAGGTTAGCAACATGA

Upstream 100 bases:

>100_bases
CAGGAGAGAAATATTACTACTGGATTCAACGCTAAGAGAGGGGGAACAAACACCAGGAGTTAACTACACACCAGAACAGA
GACTTGAGATAGCTTTAGCC

Downstream 100 bases:

>100_bases
CATTAATTGAAGAAATTCTCGGTGGAAAATCTGGAGAAAGTGTTGTTAGAAGAGTGGATTTAGTATACGCTCATGATGGG
ACAATGCCCTTAATAATAGA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDEFGVEFIEVGHPAVSRDVFEGIKLVVQQGLKANLLAHSRALISDVDYVLKAGTDWIGIFFCLSNHCLTKRFGITLEQA
LDRISRAIEYAKDHGLKVRFTPEDTTRTEWKNLVAALNLAKELKVDRVSIADTTGSAHPLKFYMLVKNVTEFGIPVNVHC
HNDLGLALANAIMGIEAGATLVDATINGLGERAGIVDLAQIATVLYYHYGIKKYKLEMLYYLSQLLTKITGIRPQQNYPI
VGENAFTHKAGLHVSAVIKDPTFYEFLPAETFGRERRIYLDRFAGRDTIRFYLRKAGIENENIVEELLRRVKESREPYTW
EKLIEEARRLAT

Sequences:

>Translated_332_residues
MDEFGVEFIEVGHPAVSRDVFEGIKLVVQQGLKANLLAHSRALISDVDYVLKAGTDWIGIFFCLSNHCLTKRFGITLEQA
LDRISRAIEYAKDHGLKVRFTPEDTTRTEWKNLVAALNLAKELKVDRVSIADTTGSAHPLKFYMLVKNVTEFGIPVNVHC
HNDLGLALANAIMGIEAGATLVDATINGLGERAGIVDLAQIATVLYYHYGIKKYKLEMLYYLSQLLTKITGIRPQQNYPI
VGENAFTHKAGLHVSAVIKDPTFYEFLPAETFGRERRIYLDRFAGRDTIRFYLRKAGIENENIVEELLRRVKESREPYTW
EKLIEEARRLAT
>Mature_332_residues
MDEFGVEFIEVGHPAVSRDVFEGIKLVVQQGLKANLLAHSRALISDVDYVLKAGTDWIGIFFCLSNHCLTKRFGITLEQA
LDRISRAIEYAKDHGLKVRFTPEDTTRTEWKNLVAALNLAKELKVDRVSIADTTGSAHPLKFYMLVKNVTEFGIPVNVHC
HNDLGLALANAIMGIEAGATLVDATINGLGERAGIVDLAQIATVLYYHYGIKKYKLEMLYYLSQLLTKITGIRPQQNYPI
VGENAFTHKAGLHVSAVIKDPTFYEFLPAETFGRERRIYLDRFAGRDTIRFYLRKAGIENENIVEELLRRVKESREPYTW
EKLIEEARRLAT

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=315, Percent_Identity=27.3015873015873, Blast_Score=129, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320019, Length=319, Percent_Identity=30.0940438871473, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320071, Length=319, Percent_Identity=30.0940438871473, Blast_Score=166, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24582381, Length=205, Percent_Identity=24.390243902439, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011872
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 37561; Mature: 37561

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEFGVEFIEVGHPAVSRDVFEGIKLVVQQGLKANLLAHSRALISDVDYVLKAGTDWIGI
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FFCLSNHCLTKRFGITLEQALDRISRAIEYAKDHGLKVRFTPEDTTRTEWKNLVAALNLA
HHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHH
KELKVDRVSIADTTGSAHPLKFYMLVKNVTEFGIPVNVHCHNDLGLALANAIMGIEAGAT
HHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCH
LVDATINGLGERAGIVDLAQIATVLYYHYGIKKYKLEMLYYLSQLLTKITGIRPQQNYPI
HHHHHHHHCCHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
VGENAFTHKAGLHVSAVIKDPTFYEFLPAETFGRERRIYLDRFAGRDTIRFYLRKAGIEN
ECCCCCCHHCCCEEEEEECCCCHHHCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCC
ENIVEELLRRVKESREPYTWEKLIEEARRLAT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDEFGVEFIEVGHPAVSRDVFEGIKLVVQQGLKANLLAHSRALISDVDYVLKAGTDWIGI
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FFCLSNHCLTKRFGITLEQALDRISRAIEYAKDHGLKVRFTPEDTTRTEWKNLVAALNLA
HHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHH
KELKVDRVSIADTTGSAHPLKFYMLVKNVTEFGIPVNVHCHNDLGLALANAIMGIEAGAT
HHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCH
LVDATINGLGERAGIVDLAQIATVLYYHYGIKKYKLEMLYYLSQLLTKITGIRPQQNYPI
HHHHHHHHCCHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
VGENAFTHKAGLHVSAVIKDPTFYEFLPAETFGRERRIYLDRFAGRDTIRFYLRKAGIEN
ECCCCCCHHCCCEEEEEECCCCHHHCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCC
ENIVEELLRRVKESREPYTWEKLIEEARRLAT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]