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The map label for this gene is gidA [H]

Identifier: 18311636

GI number: 18311636

Start: 3025016

End: 3026908

Strand: Reverse

Name: gidA [H]

Synonym: CPE2654

Alternate gene names: 18311636

Gene position: 3026908-3025016 (Counterclockwise)

Preceding gene: 18311637

Following gene: 18311635

Centisome position: 99.85

GC content: 32.49

Gene sequence:

>1893_bases
ATGAGATATAATGCAGGAAGTTATGATATAGTTGTTATAGGTGCTGGTCATGCAGGTTGTGAGGCAGGATTAGCGGCAGC
TAGAATGGGATGTAAAACTTTAGTTTGTACATTAACATTAGATTCCGTTGCTATGATGCCATGTAACCCTAATATAGGTG
GAACTGCTAAGGGACATCTAGTTAGAGAAATAGATGCTTTAGGTGGAGAAATGGGAGTAAATATAGACCATACATTTATC
CAATCTAAAATGTTAAATACATCAAAAGGACCTGCTGTACACTCTCTAAGAGCACAAGCTGATAAAAAGAAATACCAAGA
AAGAATGAAAAAGGTTTTAGAAACACAAGAAAATCTTCACTTAAGACAATTAGAGGTAGTTTCTGTAGAAGTTGAAGATG
GAAAAGTTAAAGGTGTTCTAACTAAAAATGGTGCATTTTTTGAGTGTAAAGCTGTTATAATGACAAGTGGAACATACTTA
CAAAGTAGAATAATAATCGGAGATGTTAGTTATAGCCAAGGTCCAAATGGACTTAGCAATGCTAATGAACTTTCAAAATC
ATTAATAGATTTAGGAATTGACCTTAGAAGATTTAAAACTGGTACTCCAGCTAGAATCAATAAGAGAAGTGTTGATTTCT
CAAAAATGATAGAACAACCAGGAGATGAGGAAATAATACCATTCTCATTTATAAGTGGAAATATAGATAGAGACCAAGTT
TCATGTTGGTTAACATATACTAACGAGGAAACTCATAAGGTAATTCAAGAGAATATACATAGATCACCAATGTATAATGG
AAGCATAAAAGGTGTTGGCCCAAGATATTGTCCATCAATAGAAGACAAGGTAATGAGATTCCAAGATAAAGATAGACATC
AAATCTTCATAGAACCAGAAGGTGATGATACAGAAGAAATGTATGTTGGAGGAATGTCATCATCCCTTCCAGAAGATGTT
CAAGTTCAAATGATAAAAACAGTTCCAGGTCTTGAAAATGCTGAGATTATGAGAACTGCATATGCAATAGAATATGATTG
TATAGATCCAACACAATTAAAGGCTTCACTAGAATTTAAAAACATTGATGGATTCTTTAGTGCTGGACAAATTAATGGTA
GCTCAGGATATGAAGAAGCTGGAGCTCAAGGAATAGTAGCTGGTATAAATGCAGCGTTAAAAGTTCAAGGGAAAGATCCT
ATGATACTTACAAGATCAGATGGATATGTAGGAGTTTTAATTGATGATTTAATTACTAAAGGTACTAATGAGCCTTATAG
AATGATGACATCAAGAGCTGAATATAGATTATTATTAAGACAAGATAATGCTGATTTCAGATTAACTGAAATTGGACACA
ATGTTGGATTAGTTACAGAGGAAAGATGGAATAAGTTTAAGGAAAGAAAGCAAAACTTAGAACGTGAGCTTGAGAGACTT
AAAGAACTTCAAATAACTAATAAAACAGAAAATAATGAGAAAATAGTTGAATTAGGTTCAACAGAGCTTAAAAAGCCAAT
AAGAATGTATGAACTTATAAAAAGACCAGAGTTAGACTACTTCTCATTAGCATGTTTAGATCCTGAAAGACCTGATTTAC
CAAAGGATATTGGGGATCAAATAAACATAATTGCTAGATACGAAGGATACATTCAAACTCAATTAGAACAAGTTGCTCAA
TTTAAAAAGTTTGAGAAAAAAGTTCTTCCAGAGGATTTAGATTATAATGACGTAAATAGTTTAAGAATAGAAGCTATACA
AAAGCTAAATAAAATTAGACCTTTAAATATAGGTCAAGCTTCAAGAATATCAGGGGTATCCCCAGCAGATATATCAGTTC
TGTTAATATTCTTAGAGCATTATAGAAAAACTGGAAAAAATACTGATAACTAA

Upstream 100 bases:

>100_bases
TAAGAGCCTTAGGGGAAATCACTGGAGCAGAGTTAGAGGAAGACTTAGTAAACAAAATATTTGCAGAATTTTGTTGTGGA
AAATAGGAGAATGATGAAAT

Downstream 100 bases:

>100_bases
AAGTTATTCAGAAGTTTTCAACAGCACCTGTGGATAAGTCTTGTGTAAAACTTAAAAAAGGTGCTGTTTTACCCCTTTTA
AAAAGTTATCCACATGTGGA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MRYNAGSYDIVVIGAGHAGCEAGLAAARMGCKTLVCTLTLDSVAMMPCNPNIGGTAKGHLVREIDALGGEMGVNIDHTFI
QSKMLNTSKGPAVHSLRAQADKKKYQERMKKVLETQENLHLRQLEVVSVEVEDGKVKGVLTKNGAFFECKAVIMTSGTYL
QSRIIIGDVSYSQGPNGLSNANELSKSLIDLGIDLRRFKTGTPARINKRSVDFSKMIEQPGDEEIIPFSFISGNIDRDQV
SCWLTYTNEETHKVIQENIHRSPMYNGSIKGVGPRYCPSIEDKVMRFQDKDRHQIFIEPEGDDTEEMYVGGMSSSLPEDV
QVQMIKTVPGLENAEIMRTAYAIEYDCIDPTQLKASLEFKNIDGFFSAGQINGSSGYEEAGAQGIVAGINAALKVQGKDP
MILTRSDGYVGVLIDDLITKGTNEPYRMMTSRAEYRLLLRQDNADFRLTEIGHNVGLVTEERWNKFKERKQNLERELERL
KELQITNKTENNEKIVELGSTELKKPIRMYELIKRPELDYFSLACLDPERPDLPKDIGDQINIIARYEGYIQTQLEQVAQ
FKKFEKKVLPEDLDYNDVNSLRIEAIQKLNKIRPLNIGQASRISGVSPADISVLLIFLEHYRKTGKNTDN

Sequences:

>Translated_630_residues
MRYNAGSYDIVVIGAGHAGCEAGLAAARMGCKTLVCTLTLDSVAMMPCNPNIGGTAKGHLVREIDALGGEMGVNIDHTFI
QSKMLNTSKGPAVHSLRAQADKKKYQERMKKVLETQENLHLRQLEVVSVEVEDGKVKGVLTKNGAFFECKAVIMTSGTYL
QSRIIIGDVSYSQGPNGLSNANELSKSLIDLGIDLRRFKTGTPARINKRSVDFSKMIEQPGDEEIIPFSFISGNIDRDQV
SCWLTYTNEETHKVIQENIHRSPMYNGSIKGVGPRYCPSIEDKVMRFQDKDRHQIFIEPEGDDTEEMYVGGMSSSLPEDV
QVQMIKTVPGLENAEIMRTAYAIEYDCIDPTQLKASLEFKNIDGFFSAGQINGSSGYEEAGAQGIVAGINAALKVQGKDP
MILTRSDGYVGVLIDDLITKGTNEPYRMMTSRAEYRLLLRQDNADFRLTEIGHNVGLVTEERWNKFKERKQNLERELERL
KELQITNKTENNEKIVELGSTELKKPIRMYELIKRPELDYFSLACLDPERPDLPKDIGDQINIIARYEGYIQTQLEQVAQ
FKKFEKKVLPEDLDYNDVNSLRIEAIQKLNKIRPLNIGQASRISGVSPADISVLLIFLEHYRKTGKNTDN
>Mature_630_residues
MRYNAGSYDIVVIGAGHAGCEAGLAAARMGCKTLVCTLTLDSVAMMPCNPNIGGTAKGHLVREIDALGGEMGVNIDHTFI
QSKMLNTSKGPAVHSLRAQADKKKYQERMKKVLETQENLHLRQLEVVSVEVEDGKVKGVLTKNGAFFECKAVIMTSGTYL
QSRIIIGDVSYSQGPNGLSNANELSKSLIDLGIDLRRFKTGTPARINKRSVDFSKMIEQPGDEEIIPFSFISGNIDRDQV
SCWLTYTNEETHKVIQENIHRSPMYNGSIKGVGPRYCPSIEDKVMRFQDKDRHQIFIEPEGDDTEEMYVGGMSSSLPEDV
QVQMIKTVPGLENAEIMRTAYAIEYDCIDPTQLKASLEFKNIDGFFSAGQINGSSGYEEAGAQGIVAGINAALKVQGKDP
MILTRSDGYVGVLIDDLITKGTNEPYRMMTSRAEYRLLLRQDNADFRLTEIGHNVGLVTEERWNKFKERKQNLERELERL
KELQITNKTENNEKIVELGSTELKKPIRMYELIKRPELDYFSLACLDPERPDLPKDIGDQINIIARYEGYIQTQLEQVAQ
FKKFEKKVLPEDLDYNDVNSLRIEAIQKLNKIRPLNIGQASRISGVSPADISVLLIFLEHYRKTGKNTDN

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=637, Percent_Identity=41.287284144427, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI19882217, Length=662, Percent_Identity=39.7280966767372, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI183227703, Length=677, Percent_Identity=38.8478581979321, Blast_Score=423, Evalue=1e-118,
Organism=Escherichia coli, GI2367273, Length=615, Percent_Identity=50.4065040650407, Blast_Score=605, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI17534255, Length=618, Percent_Identity=39.6440129449838, Blast_Score=465, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6321202, Length=620, Percent_Identity=41.7741935483871, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24658174, Length=641, Percent_Identity=39.9375975039002, Blast_Score=435, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70626; Mature: 70626

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYNAGSYDIVVIGAGHAGCEAGLAAARMGCKTLVCTLTLDSVAMMPCNPNIGGTAKGHL
CCCCCCCEEEEEEECCCCCHHHHHHHHHHCHHEEEEEEEECCEEEECCCCCCCCCCCCHH
VREIDALGGEMGVNIDHTFIQSKMLNTSKGPAVHSLRAQADKKKYQERMKKVLETQENLH
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LRQLEVVSVEVEDGKVKGVLTKNGAFFECKAVIMTSGTYLQSRIIIGDVSYSQGPNGLSN
EEEEEEEEEEECCCEEEEEEECCCCEEEEEEEEEECCCEEEEEEEEEECCCCCCCCCCCC
ANELSKSLIDLGIDLRRFKTGTPARINKRSVDFSKMIEQPGDEEIIPFSFISGNIDRDQV
HHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCE
SCWLTYTNEETHKVIQENIHRSPMYNGSIKGVGPRYCPSIEDKVMRFQDKDRHQIFIEPE
EEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECC
GDDTEEMYVGGMSSSLPEDVQVQMIKTVPGLENAEIMRTAYAIEYDCIDPTQLKASLEFK
CCCCHHHEECCCCCCCCCHHHEEHEECCCCCCCHHHHHHEEEEEECCCCHHHHEEEEEEE
NIDGFFSAGQINGSSGYEEAGAQGIVAGINAALKVQGKDPMILTRSDGYVGVLIDDLITK
CCCCCEECCEECCCCCCHHHCCCEEEEECCEEEEECCCCCEEEEECCCEEEEEHHHHHHC
GTNEPYRMMTSRAEYRLLLRQDNADFRLTEIGHNVGLVTEERWNKFKERKQNLERELERL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
KELQITNKTENNEKIVELGSTELKKPIRMYELIKRPELDYFSLACLDPERPDLPKDIGDQ
HHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHCCCC
INIIARYEGYIQTQLEQVAQFKKFEKKVLPEDLDYNDVNSLRIEAIQKLNKIRPLNIGQA
EEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
SRISGVSPADISVLLIFLEHYRKTGKNTDN
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRYNAGSYDIVVIGAGHAGCEAGLAAARMGCKTLVCTLTLDSVAMMPCNPNIGGTAKGHL
CCCCCCCEEEEEEECCCCCHHHHHHHHHHCHHEEEEEEEECCEEEECCCCCCCCCCCCHH
VREIDALGGEMGVNIDHTFIQSKMLNTSKGPAVHSLRAQADKKKYQERMKKVLETQENLH
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LRQLEVVSVEVEDGKVKGVLTKNGAFFECKAVIMTSGTYLQSRIIIGDVSYSQGPNGLSN
EEEEEEEEEEECCCEEEEEEECCCCEEEEEEEEEECCCEEEEEEEEEECCCCCCCCCCCC
ANELSKSLIDLGIDLRRFKTGTPARINKRSVDFSKMIEQPGDEEIIPFSFISGNIDRDQV
HHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCE
SCWLTYTNEETHKVIQENIHRSPMYNGSIKGVGPRYCPSIEDKVMRFQDKDRHQIFIEPE
EEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECC
GDDTEEMYVGGMSSSLPEDVQVQMIKTVPGLENAEIMRTAYAIEYDCIDPTQLKASLEFK
CCCCHHHEECCCCCCCCCHHHEEHEECCCCCCCHHHHHHEEEEEECCCCHHHHEEEEEEE
NIDGFFSAGQINGSSGYEEAGAQGIVAGINAALKVQGKDPMILTRSDGYVGVLIDDLITK
CCCCCEECCEECCCCCCHHHCCCEEEEECCEEEEECCCCCEEEEECCCEEEEEHHHHHHC
GTNEPYRMMTSRAEYRLLLRQDNADFRLTEIGHNVGLVTEERWNKFKERKQNLERELERL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
KELQITNKTENNEKIVELGSTELKKPIRMYELIKRPELDYFSLACLDPERPDLPKDIGDQ
HHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHCCCC
INIIARYEGYIQTQLEQVAQFKKFEKKVLPEDLDYNDVNSLRIEAIQKLNKIRPLNIGQA
EEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
SRISGVSPADISVLLIFLEHYRKTGKNTDN
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA