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The map label for this gene is trmE

Identifier: 18311637

GI number: 18311637

Start: 3026923

End: 3028299

Strand: Reverse

Name: trmE

Synonym: CPE2655

Alternate gene names: 18311637

Gene position: 3028299-3026923 (Counterclockwise)

Preceding gene: 18311638

Following gene: 18311636

Centisome position: 99.9

GC content: 30.72

Gene sequence:

>1377_bases
ATGAAAGAATTCGATACTATTGCTGCTATAGCTACAGCATTAGGAGAAGGTGGAATAGCTATAATTAGAGTATCAGGAAA
TAAAGCTCTTGAGATTGTAAATAAAATATTCAGAGGAATAAACGGAAAAGATTTATTAGATATAAAACCATATACTATGA
GATATGGTCATATGATAGATGAAAATAATGAGATTATAGATGAAGTTATAGTTAGTTTCATGAAAGGTCCTAGAAGCTTT
ACAGCTGAGGATACAGTAGAAATAAACTGTCATGGTGGTATTGTAGCTACTAATAAAGTTCTACAAAATGTAATAAAAGC
TGGTGCAAGACTTGCTGAGCCAGGTGAATTTACAAAAAGAGCTTTTTTAAATGGAAGAATAGATTTAAGCCAAGCAGAAG
CGGTTATGGATATAATAACTGCTAAGACTGAACTTTCAATGAAATCAGCTATGACTCAAAGTCAAGGAAGACTTTCTACT
GAGATAAACAATCTTAGAAAAGAGGCTTTAGATATATTAGCTCTAATTGAATATGCTGTTGACTTTACAGAGGATGACGA
AGAACCAGATGAAACTATACCAGTTAAGGTAAAAGAGGATGTTATAACTCTTAGAGGTAAAGTAAATAATTTAATTGATA
CTGCTGATGAAGGTAAACTTATTAGAGATGGTTTAAGCATGGTTATAGTAGGAAAACCTAACGTAGGAAAATCATCACTT
TTAAATGCTCTATTAAATGAAAAGAGAGCTATTGTAACTGACATAGCAGGAACAACAAGGGACGTAATAGAAGAATATAT
AAATTTAGATGGAATACCTGTTAGACTAGTAGATACAGCGGGAATCAGAGAAACTGAAGATGTTGTAGAGAAAATAGGTG
TAGAAAAATCTAAAGAAAAAATCAATGAAGCTGATTTAGTTATACTAATGTTAGATACAAGTAGAGAACTAGATGAGGAA
GACAAGGAAATCATTGATTACATTAAAGATAGAAAATACATAGTACTTTTAAATAAGGTTGATTTAGATAGAAAGTTATC
CTCAGAAATTGTGGATAACTTAGAAAATAAAATAGAACTATCTGCAAAAACTGGTTTTGGTATAGATGATTTAAAATCAA
AGATAAAAGATTTATTCTTTAATGGATCAATTGATGCTGAAAGTGTAATGGTAACTAATACTAGACATAAAGAGGCTTTA
TATAGAGCTTCTGAAAACTTAGATGGTGCATTAAATGGACTTAATAATAATGAGTTTTTAGATCTAGTATCAATTTATGT
TACATCTGCTTTAAGAGCCTTAGGGGAAATCACTGGAGCAGAGTTAGAGGAAGACTTAGTAAACAAAATATTTGCAGAAT
TTTGTTGTGGAAAATAG

Upstream 100 bases:

>100_bases
TGCCATAAATAATATGATTTAAAATTTTGACTTATTCTATTTTGTTGGGTAAAATCAATATTAGTTCAAATTTGAGAATA
TCATTATAAAGGAGGAGCTT

Downstream 100 bases:

>100_bases
GAGAATGATGAAATATGAGATATAATGCAGGAAGTTATGATATAGTTGTTATAGGTGCTGGTCATGCAGGTTGTGAGGCA
GGATTAGCGGCAGCTAGAAT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKEFDTIAAIATALGEGGIAIIRVSGNKALEIVNKIFRGINGKDLLDIKPYTMRYGHMIDENNEIIDEVIVSFMKGPRSF
TAEDTVEINCHGGIVATNKVLQNVIKAGARLAEPGEFTKRAFLNGRIDLSQAEAVMDIITAKTELSMKSAMTQSQGRLST
EINNLRKEALDILALIEYAVDFTEDDEEPDETIPVKVKEDVITLRGKVNNLIDTADEGKLIRDGLSMVIVGKPNVGKSSL
LNALLNEKRAIVTDIAGTTRDVIEEYINLDGIPVRLVDTAGIRETEDVVEKIGVEKSKEKINEADLVILMLDTSRELDEE
DKEIIDYIKDRKYIVLLNKVDLDRKLSSEIVDNLENKIELSAKTGFGIDDLKSKIKDLFFNGSIDAESVMVTNTRHKEAL
YRASENLDGALNGLNNNEFLDLVSIYVTSALRALGEITGAELEEDLVNKIFAEFCCGK

Sequences:

>Translated_458_residues
MKEFDTIAAIATALGEGGIAIIRVSGNKALEIVNKIFRGINGKDLLDIKPYTMRYGHMIDENNEIIDEVIVSFMKGPRSF
TAEDTVEINCHGGIVATNKVLQNVIKAGARLAEPGEFTKRAFLNGRIDLSQAEAVMDIITAKTELSMKSAMTQSQGRLST
EINNLRKEALDILALIEYAVDFTEDDEEPDETIPVKVKEDVITLRGKVNNLIDTADEGKLIRDGLSMVIVGKPNVGKSSL
LNALLNEKRAIVTDIAGTTRDVIEEYINLDGIPVRLVDTAGIRETEDVVEKIGVEKSKEKINEADLVILMLDTSRELDEE
DKEIIDYIKDRKYIVLLNKVDLDRKLSSEIVDNLENKIELSAKTGFGIDDLKSKIKDLFFNGSIDAESVMVTNTRHKEAL
YRASENLDGALNGLNNNEFLDLVSIYVTSALRALGEITGAELEEDLVNKIFAEFCCGK
>Mature_458_residues
MKEFDTIAAIATALGEGGIAIIRVSGNKALEIVNKIFRGINGKDLLDIKPYTMRYGHMIDENNEIIDEVIVSFMKGPRSF
TAEDTVEINCHGGIVATNKVLQNVIKAGARLAEPGEFTKRAFLNGRIDLSQAEAVMDIITAKTELSMKSAMTQSQGRLST
EINNLRKEALDILALIEYAVDFTEDDEEPDETIPVKVKEDVITLRGKVNNLIDTADEGKLIRDGLSMVIVGKPNVGKSSL
LNALLNEKRAIVTDIAGTTRDVIEEYINLDGIPVRLVDTAGIRETEDVVEKIGVEKSKEKINEADLVILMLDTSRELDEE
DKEIIDYIKDRKYIVLLNKVDLDRKLSSEIVDNLENKIELSAKTGFGIDDLKSKIKDLFFNGSIDAESVMVTNTRHKEAL
YRASENLDGALNGLNNNEFLDLVSIYVTSALRALGEITGAELEEDLVNKIFAEFCCGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=474, Percent_Identity=32.0675105485232, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI193082988, Length=505, Percent_Identity=30.4950495049505, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI193082993, Length=463, Percent_Identity=31.9654427645788, Blast_Score=205, Evalue=7e-53,
Organism=Escherichia coli, GI2367268, Length=465, Percent_Identity=37.4193548387097, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI87082120, Length=183, Percent_Identity=30.6010928961749, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=468, Percent_Identity=30.1282051282051, Blast_Score=198, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6323665, Length=506, Percent_Identity=30.0395256916996, Blast_Score=204, Evalue=3e-53,
Organism=Drosophila melanogaster, GI45550813, Length=479, Percent_Identity=31.7327766179541, Blast_Score=230, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_CLOP1 (Q0TLZ4)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_697346.1
- ProteinModelPortal:   Q0TLZ4
- SMR:   Q0TLZ4
- STRING:   Q0TLZ4
- GeneID:   4203662
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_2992
- TIGR:   CPF_2992
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   CPER195103:CPF_2992-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50616; Mature: 50616

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 87-87 BINDING 126-126 BINDING 458-458

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEFDTIAAIATALGEGGIAIIRVSGNKALEIVNKIFRGINGKDLLDIKPYTMRYGHMID
CCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEECCCHHHHHCCEEC
ENNEIIDEVIVSFMKGPRSFTAEDTVEINCHGGIVATNKVLQNVIKAGARLAEPGEFTKR
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRIDLSQAEAVMDIITAKTELSMKSAMTQSQGRLSTEINNLRKEALDILALIEYAV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
DFTEDDEEPDETIPVKVKEDVITLRGKVNNLIDTADEGKLIRDGLSMVIVGKPNVGKSSL
CCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEECCCCCHHHH
LNALLNEKRAIVTDIAGTTRDVIEEYINLDGIPVRLVDTAGIRETEDVVEKIGVEKSKEK
HHHHHHCCHHEEHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHH
INEADLVILMLDTSRELDEEDKEIIDYIKDRKYIVLLNKVDLDRKLSSEIVDNLENKIEL
CCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHEEE
SAKTGFGIDDLKSKIKDLFFNGSIDAESVMVTNTRHKEALYRASENLDGALNGLNNNEFL
EECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCCHHHCCCCCCHHH
DLVSIYVTSALRALGEITGAELEEDLVNKIFAEFCCGK
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEFDTIAAIATALGEGGIAIIRVSGNKALEIVNKIFRGINGKDLLDIKPYTMRYGHMID
CCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEECCCHHHHHCCEEC
ENNEIIDEVIVSFMKGPRSFTAEDTVEINCHGGIVATNKVLQNVIKAGARLAEPGEFTKR
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRIDLSQAEAVMDIITAKTELSMKSAMTQSQGRLSTEINNLRKEALDILALIEYAV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
DFTEDDEEPDETIPVKVKEDVITLRGKVNNLIDTADEGKLIRDGLSMVIVGKPNVGKSSL
CCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEECCCCCHHHH
LNALLNEKRAIVTDIAGTTRDVIEEYINLDGIPVRLVDTAGIRETEDVVEKIGVEKSKEK
HHHHHHCCHHEEHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHH
INEADLVILMLDTSRELDEEDKEIIDYIKDRKYIVLLNKVDLDRKLSSEIVDNLENKIEL
CCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHEEE
SAKTGFGIDDLKSKIKDLFFNGSIDAESVMVTNTRHKEALYRASENLDGALNGLNNNEFL
EECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCCHHHCCCCCCHHH
DLVSIYVTSALRALGEITGAELEEDLVNKIFAEFCCGK
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA