Click here to switch to the map view.

The map label for this gene is manX [H]

Identifier: 18311614

GI number: 18311614

Start: 3005839

End: 3006327

Strand: Reverse

Name: manX [H]

Synonym: CPE2632

Alternate gene names: 18311614

Gene position: 3006327-3005839 (Counterclockwise)

Preceding gene: 18311615

Following gene: 18311613

Centisome position: 99.17

GC content: 32.52

Gene sequence:

>489_bases
ATGCCAATAGTACATTTCAGAGTTGACGGAAGACTTATTCATGGACAAGTAGCCTTGGCTTGGTCTAAAATGACAAATCC
AAATCATATTATAGTTGCCAATGATATGGTTGCTAGTGATCCAAATCAACAAGCAATACTAAAATTAGCTGCTCCAAAGC
AAGTAAGAATATCAATGCAGCCAGTTGATAAGGTTATTGCATACTTAAATAGAGGACTAGCTCCAAATGAGAGAGTTTTT
CTATTAGTTAAGGATGTGGTAGATGCTAATAGGATTTATGAGGGTGGATTTAAATATATGGACCTTAATATAGGCAATAT
GACTCATAAACCAAATGCTAAAAAATTAAGTGAAAAACTATTTGTTACAGAAAAAGAGTTTGAAGCATTAAAAAGTTTGC
TAGATAAAAATGTTAATTGCTACTTCCAAATGATTCCGACAGACTCAAAGCAAGATATTAGACCGTTAGTAGAGGTTTTT
AAAAGTTAA

Upstream 100 bases:

>100_bases
TCGTTTAAAATAATAATTTAATTATAAAATTATTATAAAGATGTATTACTAGATTTTTAGAGTCATATAATAGTACAAAC
AATATTAAGGAGGATTTAAA

Downstream 100 bases:

>100_bases
TTATTTTAAGGGGATTATACAATTTAATAATTAGGGGGATATGTTTATGCAAGAAATAGGAATTATACAAATTTTACTAC
TCTCTGTATGGGCTTTCCTA

Product: PTS system protein

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MPIVHFRVDGRLIHGQVALAWSKMTNPNHIIVANDMVASDPNQQAILKLAAPKQVRISMQPVDKVIAYLNRGLAPNERVF
LLVKDVVDANRIYEGGFKYMDLNIGNMTHKPNAKKLSEKLFVTEKEFEALKSLLDKNVNCYFQMIPTDSKQDIRPLVEVF
KS

Sequences:

>Translated_162_residues
MPIVHFRVDGRLIHGQVALAWSKMTNPNHIIVANDMVASDPNQQAILKLAAPKQVRISMQPVDKVIAYLNRGLAPNERVF
LLVKDVVDANRIYEGGFKYMDLNIGNMTHKPNAKKLSEKLFVTEKEFEALKSLLDKNVNCYFQMIPTDSKQDIRPLVEVF
KS
>Mature_161_residues
PIVHFRVDGRLIHGQVALAWSKMTNPNHIIVANDMVASDPNQQAILKLAAPKQVRISMQPVDKVIAYLNRGLAPNERVFL
LVKDVVDANRIYEGGFKYMDLNIGNMTHKPNAKKLSEKLFVTEKEFEALKSLLDKNVNCYFQMIPTDSKQDIRPLVEVFK
S

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=157, Percent_Identity=35.6687898089172, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI87082217, Length=162, Percent_Identity=31.4814814814815, Blast_Score=89, Evalue=1e-19,
Organism=Escherichia coli, GI1789527, Length=148, Percent_Identity=30.4054054054054, Blast_Score=80, Evalue=7e-17,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 18406; Mature: 18275

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIVHFRVDGRLIHGQVALAWSKMTNPNHIIVANDMVASDPNQQAILKLAAPKQVRISMQ
CCEEEEEECCEEEECEEEEEEECCCCCCEEEEECCEECCCCCHHEEEEECCCCEEEEEEC
PVDKVIAYLNRGLAPNERVFLLVKDVVDANRIYEGGFKYMDLNIGNMTHKPNAKKLSEKL
CHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCEECCCCEEEEEECCCCCCCCCHHHHHHHH
FVTEKEFEALKSLLDKNVNCYFQMIPTDSKQDIRPLVEVFKS
CCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PIVHFRVDGRLIHGQVALAWSKMTNPNHIIVANDMVASDPNQQAILKLAAPKQVRISMQ
CEEEEEECCEEEECEEEEEEECCCCCCEEEEECCEECCCCCHHEEEEECCCCEEEEEEC
PVDKVIAYLNRGLAPNERVFLLVKDVVDANRIYEGGFKYMDLNIGNMTHKPNAKKLSEKL
CHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCEECCCCEEEEEECCCCCCCCCHHHHHHHH
FVTEKEFEALKSLLDKNVNCYFQMIPTDSKQDIRPLVEVFKS
CCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]