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The map label for this gene is purA

Identifier: 18311604

GI number: 18311604

Start: 2996805

End: 2998091

Strand: Reverse

Name: purA

Synonym: CPE2622

Alternate gene names: 18311604

Gene position: 2998091-2996805 (Counterclockwise)

Preceding gene: 18311609

Following gene: 18311603

Centisome position: 98.9

GC content: 33.8

Gene sequence:

>1287_bases
ATGTCAGCATTTGTTGTATTAGGAGCTCAATGGGGAGACGAAGGAAAAGGAAAGATGACAGATTACTTAGCAGAAGAGGC
AGAAGTAGTTGTTAGATTCCAAGGAGGAAATAACGCTGGACATACAGTTGAAGTTGAAGACAAACAATACAAGTTACACT
TAATACCTTCAGGAATATTACATGACGAGAAATTAAACGTAATAGGAAATGGTGTTGTAGTAGATCCTAAAGCACTATTT
ACAGAAATAGATTATTTAGAAGGCTTAGGAGTAAAAGTAACTCCAGAGAAATTAATAGTTAGTGATAGAGCACATCTTAT
AATGCCATACCACATAACTTTAGATAAGTTAAAAGAAAAAGCTAGAGGAAAAAATGATATAGGAACTACTTGCAAAGGAA
TAGGTCCTTGTTATACAGATAAATATGAAAGATCAGGAATAAGAGTTTGTGACCTTATGCACAAAGATTCTTTCGCTGAA
AAATTAAGAATAAACATCGAAATGAAAAATGGATACATCAAATTATTAGGTGGAGAAGAGTTAAACTTCGATGAAATATA
TAATGAATATATGGCATTTGCTGAAAGATTAAGACCATATGTTAAAGATACTTCAGTTGAAATATACAATGCTATCCAAG
CTGATAAAAATGTATTATTTGAAGGAGCTCAAGGAATGCTTCTTGATATAGACTATGGAACTTATCCATATGTTACATCA
TCAAACACAACTTCATGTGGAGTTGCTAGTGGAGCAGGTATAGGACCAAACATGGTAACTAATGCTGTTGGTATAGCTAA
AGCTTACACTACAAGAGTTGGTAAAGGACCATTCCCAACAGAGCTTGAAAACGAAACTGGTGATTGGATAAGAGAAAAAG
GTCATGAGTACGGAGTAACTACAGGAAGATCAAGAAGATGTGGTTGGTTAGACCTTGTTATCTTAAAAACTACTACAAGA
GTTTGTGGATTAACTTCATTAGTAGTAACTAAAATAGATACATTAGCTGGATTAGATAAAATACAAATGTGTGTTGGATA
TGAATTAGATGGAAAAGTTATAGATTACTTCCCAGCTAGTTTAGAAGATTTAGCTAGATGTAAACCAGTATATGAAGAGT
TCGAAGGATGGGGAGAAGAAGTTGCTGATGCAAGAAGCTACGAAGAGTTACCAGAGAACGCTAAAACTTACTTAAGAAGA
ATAGAAGAGTTCACAGGAACTAAAGTTTCTATAGTTGGTGTTGGACCAAAGAGAAATCAAACTATAAGAGTAAGAGAAGA
ACTTTAA

Upstream 100 bases:

>100_bases
CCTATAAATACGAACGTTGTCTACTCGGAAAAAGTTAATTAACCGTTATGGTTTATAAAAAAAGAATGTTAATGTTCTTT
TTATAAGGAGGAAATAAAAA

Downstream 100 bases:

>100_bases
TAAGTAGTAAAGAATAATTAGTTTAAAATAGCTTAAGATTATTTAATCTTAAGCTATTTTTTATTTTAGGGTATAATATT
TTTAAATTAATTTAGAATAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSAFVVLGAQWGDEGKGKMTDYLAEEAEVVVRFQGGNNAGHTVEVEDKQYKLHLIPSGILHDEKLNVIGNGVVVDPKALF
TEIDYLEGLGVKVTPEKLIVSDRAHLIMPYHITLDKLKEKARGKNDIGTTCKGIGPCYTDKYERSGIRVCDLMHKDSFAE
KLRINIEMKNGYIKLLGGEELNFDEIYNEYMAFAERLRPYVKDTSVEIYNAIQADKNVLFEGAQGMLLDIDYGTYPYVTS
SNTTSCGVASGAGIGPNMVTNAVGIAKAYTTRVGKGPFPTELENETGDWIREKGHEYGVTTGRSRRCGWLDLVILKTTTR
VCGLTSLVVTKIDTLAGLDKIQMCVGYELDGKVIDYFPASLEDLARCKPVYEEFEGWGEEVADARSYEELPENAKTYLRR
IEEFTGTKVSIVGVGPKRNQTIRVREEL

Sequences:

>Translated_428_residues
MSAFVVLGAQWGDEGKGKMTDYLAEEAEVVVRFQGGNNAGHTVEVEDKQYKLHLIPSGILHDEKLNVIGNGVVVDPKALF
TEIDYLEGLGVKVTPEKLIVSDRAHLIMPYHITLDKLKEKARGKNDIGTTCKGIGPCYTDKYERSGIRVCDLMHKDSFAE
KLRINIEMKNGYIKLLGGEELNFDEIYNEYMAFAERLRPYVKDTSVEIYNAIQADKNVLFEGAQGMLLDIDYGTYPYVTS
SNTTSCGVASGAGIGPNMVTNAVGIAKAYTTRVGKGPFPTELENETGDWIREKGHEYGVTTGRSRRCGWLDLVILKTTTR
VCGLTSLVVTKIDTLAGLDKIQMCVGYELDGKVIDYFPASLEDLARCKPVYEEFEGWGEEVADARSYEELPENAKTYLRR
IEEFTGTKVSIVGVGPKRNQTIRVREEL
>Mature_427_residues
SAFVVLGAQWGDEGKGKMTDYLAEEAEVVVRFQGGNNAGHTVEVEDKQYKLHLIPSGILHDEKLNVIGNGVVVDPKALFT
EIDYLEGLGVKVTPEKLIVSDRAHLIMPYHITLDKLKEKARGKNDIGTTCKGIGPCYTDKYERSGIRVCDLMHKDSFAEK
LRINIEMKNGYIKLLGGEELNFDEIYNEYMAFAERLRPYVKDTSVEIYNAIQADKNVLFEGAQGMLLDIDYGTYPYVTSS
NTTSCGVASGAGIGPNMVTNAVGIAKAYTTRVGKGPFPTELENETGDWIREKGHEYGVTTGRSRRCGWLDLVILKTTTRV
CGLTSLVVTKIDTLAGLDKIQMCVGYELDGKVIDYFPASLEDLARCKPVYEEFEGWGEEVADARSYEELPENAKTYLRRI
EEFTGTKVSIVGVGPKRNQTIRVREEL

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=46.9483568075117, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=44.8598130841121, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=45.3400503778337, Blast_Score=342, Evalue=5e-94,
Organism=Escherichia coli, GI1790620, Length=424, Percent_Identity=47.4056603773585, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=44.7552447552448, Blast_Score=349, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=44.7552447552448, Blast_Score=349, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=44.988344988345, Blast_Score=379, Evalue=1e-106,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=43.4988179669031, Blast_Score=366, Evalue=1e-101,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_CLOPE (Q8XH63)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_563538.1
- ProteinModelPortal:   Q8XH63
- SMR:   Q8XH63
- GeneID:   989276
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE2622
- NMPDR:   fig|195102.1.peg.2685
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- PhylomeDB:   Q8XH63
- ProtClustDB:   PRK01117
- BioCyc:   CPER195102:CPE2622-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47591; Mature: 47460

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2; PS00014 ER_TARGET

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 224-224 BINDING 239-239 BINDING 303-303 BINDING 305-305

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFVVLGAQWGDEGKGKMTDYLAEEAEVVVRFQGGNNAGHTVEVEDKQYKLHLIPSGIL
CCEEEEEEECCCCCCCCHHHHHHHCCEEEEEEEECCCCCCCEEEEECCEEEEEECCCCCC
HDEKLNVIGNGVVVDPKALFTEIDYLEGLGVKVTPEKLIVSDRAHLIMPYHITLDKLKEK
CCCCEEEEECCEEECCHHHHHHHHHHCCCCCEECHHHHEECCCCEEEEEEEEEHHHHHHH
ARGKNDIGTTCKGIGPCYTDKYERSGIRVCDLMHKDSFAEKLRINIEMKNGYIKLLGGEE
HCCCCCCCCCCCCCCCCCCCCHHCCCCEEEHHHHHCCCCEEEEEEEEEECCEEEEECCCC
LNFDEIYNEYMAFAERLRPYVKDTSVEIYNAIQADKNVLFEGAQGMLLDIDYGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHEEECCCCEEEEECCCCCCEEEC
SNTTSCGVASGAGIGPNMVTNAVGIAKAYTTRVGKGPFPTELENETGDWIREKGHEYGVT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCC
TGRSRRCGWLDLVILKTTTRVCGLTSLVVTKIDTLAGLDKIQMCVGYELDGKVIDYFPAS
CCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEECCCCC
LEDLARCKPVYEEFEGWGEEVADARSYEELPENAKTYLRRIEEFTGTKVSIVGVGPKRNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
TIRVREEL
EEEEEECC
>Mature Secondary Structure 
SAFVVLGAQWGDEGKGKMTDYLAEEAEVVVRFQGGNNAGHTVEVEDKQYKLHLIPSGIL
CEEEEEEECCCCCCCCHHHHHHHCCEEEEEEEECCCCCCCEEEEECCEEEEEECCCCCC
HDEKLNVIGNGVVVDPKALFTEIDYLEGLGVKVTPEKLIVSDRAHLIMPYHITLDKLKEK
CCCCEEEEECCEEECCHHHHHHHHHHCCCCCEECHHHHEECCCCEEEEEEEEEHHHHHHH
ARGKNDIGTTCKGIGPCYTDKYERSGIRVCDLMHKDSFAEKLRINIEMKNGYIKLLGGEE
HCCCCCCCCCCCCCCCCCCCCHHCCCCEEEHHHHHCCCCEEEEEEEEEECCEEEEECCCC
LNFDEIYNEYMAFAERLRPYVKDTSVEIYNAIQADKNVLFEGAQGMLLDIDYGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHEEECCCCEEEEECCCCCCEEEC
SNTTSCGVASGAGIGPNMVTNAVGIAKAYTTRVGKGPFPTELENETGDWIREKGHEYGVT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCC
TGRSRRCGWLDLVILKTTTRVCGLTSLVVTKIDTLAGLDKIQMCVGYELDGKVIDYFPAS
CCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEECCCCC
LEDLARCKPVYEEFEGWGEEVADARSYEELPENAKTYLRRIEEFTGTKVSIVGVGPKRNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
TIRVREEL
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842