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The map label for this gene is carA

Identifier: 18311555

GI number: 18311555

Start: 2947929

End: 2948978

Strand: Reverse

Name: carA

Synonym: CPE2573

Alternate gene names: 18311555

Gene position: 2948978-2947929 (Counterclockwise)

Preceding gene: 18311557

Following gene: 18311554

Centisome position: 97.28

GC content: 32.0

Gene sequence:

>1050_bases
ATGAAAGCAAAGCTTATATTAGAAAATGGCGTAGTATTTGAAGGTAAAGCCTTTGGATATTTAAAGGAGTGCGTTGGAGA
GGTAGTTTTCAACACAGGAATGACAGGATATCAGGAGGTTTTAACTGACCCTTCTTATTATGGACAAATAGTAACTATGA
CTTATCCCCTAATAGGTAACTATGGAATTAACTTAGAGGATTTAGAATCTAAGGAACCTAAAGTAAGGGGATTCATAGTG
AGAGAAAAATGTCAGTATCCAAATAATTTTAGATGTGAATTAGAGCTTGAAACTTACTTAGCTCAAAATAAGGTTTTAGG
ATTAGATGGTATAGACACAAGAGCCTTAACAAAAATCTTAAGAAATAATGGGACAATGAAGGGAATTATAGTTTTAGATA
ATTCAAACTTAGAAGATGTTAAAGATAAGTTAGAAGCTTTCTCAAATAGAGATGCAGTTTCAATAGTTTCAACTAATGAA
AAATATGAGATCTCAGGAGAAGGAAAGAAAGTAGCTATAATCGACTTTGGAATAAAGCAAAACATTATTAGAAACTTTGT
AAAAAGAGGATGTAATGTTACAGTATTCCCTTATGACTTTAAGGCAGAAGAAGTTTTAGAAATAAATCCAGACTTAGTAT
TTTTATCAAATGGACCTGGAGACCCAGAGGATATGGGAGAAGCAGTAAATGAGATAAAGAAAATAGTTGGGAAGAAACCA
ATAGTAGGAATTTGTTTAGGACATCAATTATTAGCTTTAACCTTAGGTGGAGAAACTAAGAAGCTTAAGTTTGGTCATAG
AGGATGTAATCATCCAGTTAAAGATTTAATAAATAATAGAGTTCATATAACATCACAAAATCATGGATACTATGTTGCTA
CTTTACCAGAAAATATGGAAATAACTCATGTAAGTATGAATGATGGAACAGTTGAGGGAATGAAACATAAAGAATTACCA
ATATTCTCAGTTCAATTTCACCCAGAAGCATGTCCAGGACCAAAGGATAGCGAATACATTTTTGATGAGTTTATGAAGTA
TGCACTATAA

Upstream 100 bases:

>100_bases
CCTCTTTCTTTTAATTAAATAACATTTAGAATGATGGCAATGGAATTAAGACCTTTTAAAGTGTGTTTTTTAAATATGAT
TTTAAATAGGAGGTCTAGAA

Downstream 100 bases:

>100_bases
GGAGGAAGTAATATGCCATTAAATAAAGATATAAAAAAAGTTTTAGTAATAGGTTCAGGTCCAATAATAATAGGACAAGC
GGCGGAGTTTGATTACTCAG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKAKLILENGVVFEGKAFGYLKECVGEVVFNTGMTGYQEVLTDPSYYGQIVTMTYPLIGNYGINLEDLESKEPKVRGFIV
REKCQYPNNFRCELELETYLAQNKVLGLDGIDTRALTKILRNNGTMKGIIVLDNSNLEDVKDKLEAFSNRDAVSIVSTNE
KYEISGEGKKVAIIDFGIKQNIIRNFVKRGCNVTVFPYDFKAEEVLEINPDLVFLSNGPGDPEDMGEAVNEIKKIVGKKP
IVGICLGHQLLALTLGGETKKLKFGHRGCNHPVKDLINNRVHITSQNHGYYVATLPENMEITHVSMNDGTVEGMKHKELP
IFSVQFHPEACPGPKDSEYIFDEFMKYAL

Sequences:

>Translated_349_residues
MKAKLILENGVVFEGKAFGYLKECVGEVVFNTGMTGYQEVLTDPSYYGQIVTMTYPLIGNYGINLEDLESKEPKVRGFIV
REKCQYPNNFRCELELETYLAQNKVLGLDGIDTRALTKILRNNGTMKGIIVLDNSNLEDVKDKLEAFSNRDAVSIVSTNE
KYEISGEGKKVAIIDFGIKQNIIRNFVKRGCNVTVFPYDFKAEEVLEINPDLVFLSNGPGDPEDMGEAVNEIKKIVGKKP
IVGICLGHQLLALTLGGETKKLKFGHRGCNHPVKDLINNRVHITSQNHGYYVATLPENMEITHVSMNDGTVEGMKHKELP
IFSVQFHPEACPGPKDSEYIFDEFMKYAL
>Mature_349_residues
MKAKLILENGVVFEGKAFGYLKECVGEVVFNTGMTGYQEVLTDPSYYGQIVTMTYPLIGNYGINLEDLESKEPKVRGFIV
REKCQYPNNFRCELELETYLAQNKVLGLDGIDTRALTKILRNNGTMKGIIVLDNSNLEDVKDKLEAFSNRDAVSIVSTNE
KYEISGEGKKVAIIDFGIKQNIIRNFVKRGCNVTVFPYDFKAEEVLEINPDLVFLSNGPGDPEDMGEAVNEIKKIVGKKP
IVGICLGHQLLALTLGGETKKLKFGHRGCNHPVKDLINNRVHITSQNHGYYVATLPENMEITHVSMNDGTVEGMKHKELP
IFSVQFHPEACPGPKDSEYIFDEFMKYAL

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=364, Percent_Identity=39.5604395604396, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI169790915, Length=358, Percent_Identity=37.9888268156425, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI21361331, Length=358, Percent_Identity=37.9888268156425, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1786215, Length=369, Percent_Identity=46.3414634146341, Blast_Score=321, Evalue=4e-89,
Organism=Escherichia coli, GI1789760, Length=161, Percent_Identity=26.7080745341615, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=360, Percent_Identity=40.8333333333333, Blast_Score=254, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6324878, Length=366, Percent_Identity=41.8032786885246, Blast_Score=263, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6322331, Length=387, Percent_Identity=38.2428940568475, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6322638, Length=179, Percent_Identity=29.608938547486, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24642586, Length=368, Percent_Identity=41.8478260869565, Blast_Score=260, Evalue=9e-70,
Organism=Drosophila melanogaster, GI45555749, Length=368, Percent_Identity=41.8478260869565, Blast_Score=260, Evalue=1e-69,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_CLOPE (Q8XHB2)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_563489.1
- ProteinModelPortal:   Q8XHB2
- SMR:   Q8XHB2
- GeneID:   990950
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE2573
- NMPDR:   fig|195102.1.peg.2636
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- PhylomeDB:   Q8XHB2
- ProtClustDB:   PRK12564
- BioCyc:   CPER195102:CPE2573-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 39083; Mature: 39083

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 245-245 ACT_SITE 327-327 ACT_SITE 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKLILENGVVFEGKAFGYLKECVGEVVFNTGMTGYQEVLTDPSYYGQIVTMTYPLIGN
CCCEEEEECCEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCEEEEEEEEEECC
YGINLEDLESKEPKVRGFIVREKCQYPNNFRCELELETYLAQNKVLGLDGIDTRALTKIL
CCCCHHHCCCCCCCEEEEEEEHHCCCCCCCEEEEEEHHHHHCCCEECCCCCCHHHHHHHH
RNNGTMKGIIVLDNSNLEDVKDKLEAFSNRDAVSIVSTNEKYEISGEGKKVAIIDFGIKQ
HCCCCEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCEEEEEECCHHH
NIIRNFVKRGCNVTVFPYDFKAEEVLEINPDLVFLSNGPGDPEDMGEAVNEIKKIVGKKP
HHHHHHHHCCCCEEEEECCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCC
IVGICLGHQLLALTLGGETKKLKFGHRGCNHPVKDLINNRVHITSQNHGYYVATLPENME
EEEEEECCEEEEEEECCCCEEEEECCCCCCCCHHHHHCCEEEEEECCCCEEEEECCCCCE
ITHVSMNDGTVEGMKHKELPIFSVQFHPEACPGPKDSEYIFDEFMKYAL
EEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKAKLILENGVVFEGKAFGYLKECVGEVVFNTGMTGYQEVLTDPSYYGQIVTMTYPLIGN
CCCEEEEECCEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCEEEEEEEEEECC
YGINLEDLESKEPKVRGFIVREKCQYPNNFRCELELETYLAQNKVLGLDGIDTRALTKIL
CCCCHHHCCCCCCCEEEEEEEHHCCCCCCCEEEEEEHHHHHCCCEECCCCCCHHHHHHHH
RNNGTMKGIIVLDNSNLEDVKDKLEAFSNRDAVSIVSTNEKYEISGEGKKVAIIDFGIKQ
HCCCCEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCEEEEEECCHHH
NIIRNFVKRGCNVTVFPYDFKAEEVLEINPDLVFLSNGPGDPEDMGEAVNEIKKIVGKKP
HHHHHHHHCCCCEEEEECCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCC
IVGICLGHQLLALTLGGETKKLKFGHRGCNHPVKDLINNRVHITSQNHGYYVATLPENME
EEEEEECCEEEEEEECCCCEEEEECCCCCCCCHHHHHCCEEEEEECCCCEEEEECCCCCE
ITHVSMNDGTVEGMKHKELPIFSVQFHPEACPGPKDSEYIFDEFMKYAL
EEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842