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The map label for this gene is purR [H]

Identifier: 18311474

GI number: 18311474

Start: 2853116

End: 2853919

Strand: Reverse

Name: purR [H]

Synonym: CPE2492

Alternate gene names: 18311474

Gene position: 2853919-2853116 (Counterclockwise)

Preceding gene: 18311479

Following gene: 18311473

Centisome position: 94.14

GC content: 28.98

Gene sequence:

>804_bases
ATGGAGAAGTTAAGTAGAAATAGTAGAGTATCGATTATAACAAAAGTTCTTATAGAAAATCCTAATAAAATAATAGGATT
AAATAAGTTTTCAGATCTTTTAAATGCAGCTAAATCAACTATAAGTGAAGATATAGTTATTGTTAGAGAAGTGTTATCTA
AGTTTTCTATGGGAAAAGTTGAGACAATAGCTGGTGCTGCTGGTGGAATAAGATATATTCCAGAAGTAGGAGAAGTAGAA
AAAAGAAAGTTTTTAGAAGAATTATGTGATATTGCAAGTGAAAGCAATAGAGTTGTTCCAGGTAATTTTTTATATGTTAC
AGATATAATGTTTAATCCAAGTATAATAAGTAAAGCTTCTATTATATTAGCATCTTATTTTCAAGACATGGATATAGACT
ATGTAATAACTGTGGAAACTAAGGGAGTTCCATTAGCTTACGAAGTAGCTAAATGCTTAGGAGTACAATTAATAACAGCA
AGAAGAGATAGTAAAGTAACAGAAGGATCTACAGTTTCAATAAACTATGTTTCAGGAACATCAGGCAAAATACAACAAAT
GTGTTTATCTAGAAAATCATTAAAGCAAGGAAGTAAATGCATATTTATAGATGACTTTATGAGAGGCGGCGGAACTGCTA
AAGGTTTAGTTGATTTATTAAAAGAATTCGATTCTGAACTTATGGGAATTGGAGTATTAATAGATAATAAATTATCAACA
AACAAAATAACAAAAGATTATGTCTCTTTAATTGATATTGTTGAGATAAATGAGGATGGAGTGAAAATGGTACCATCTAT
GTAA

Upstream 100 bases:

>100_bases
GAAAAATATTTATAAAAATATATGTGTTTTATAAAGAATAAAATATGAATTATAAGTGGAAAATATATACTGGGAAATTT
ATATAGGTCAGGTGATTAAT

Downstream 100 bases:

>100_bases
TTTCTACAGTTGTTGTGTTAAAGAATAATTTACAAAAAATATGAAAAAAAGAAGGATTTCTATATTTTTCATAGAATATA
CATAATAACAACAACTGGAG

Product: pur operon repressor

Products: AMP; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MEKLSRNSRVSIITKVLIENPNKIIGLNKFSDLLNAAKSTISEDIVIVREVLSKFSMGKVETIAGAAGGIRYIPEVGEVE
KRKFLEELCDIASESNRVVPGNFLYVTDIMFNPSIISKASIILASYFQDMDIDYVITVETKGVPLAYEVAKCLGVQLITA
RRDSKVTEGSTVSINYVSGTSGKIQQMCLSRKSLKQGSKCIFIDDFMRGGGTAKGLVDLLKEFDSELMGIGVLIDNKLST
NKITKDYVSLIDIVEINEDGVKMVPSM

Sequences:

>Translated_267_residues
MEKLSRNSRVSIITKVLIENPNKIIGLNKFSDLLNAAKSTISEDIVIVREVLSKFSMGKVETIAGAAGGIRYIPEVGEVE
KRKFLEELCDIASESNRVVPGNFLYVTDIMFNPSIISKASIILASYFQDMDIDYVITVETKGVPLAYEVAKCLGVQLITA
RRDSKVTEGSTVSINYVSGTSGKIQQMCLSRKSLKQGSKCIFIDDFMRGGGTAKGLVDLLKEFDSELMGIGVLIDNKLST
NKITKDYVSLIDIVEINEDGVKMVPSM
>Mature_267_residues
MEKLSRNSRVSIITKVLIENPNKIIGLNKFSDLLNAAKSTISEDIVIVREVLSKFSMGKVETIAGAAGGIRYIPEVGEVE
KRKFLEELCDIASESNRVVPGNFLYVTDIMFNPSIISKASIILASYFQDMDIDYVITVETKGVPLAYEVAKCLGVQLITA
RRDSKVTEGSTVSINYVSGTSGKIQQMCLSRKSLKQGSKCIFIDDFMRGGGTAKGLVDLLKEFDSELMGIGVLIDNKLST
NKITKDYVSLIDIVEINEDGVKMVPSM

Specific function: Controls the transcription of the pur operon for purine biosynthetic genes [H]

COG id: COG0503

COG function: function code F; Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000836
- InterPro:   IPR015265
- InterPro:   IPR010078
- InterPro:   IPR011991
- ProDom:   PD029816 [H]

Pfam domain/function: PF00156 Pribosyltran; PF09182 PuR_N [H]

EC number: 2.4.2.7 [C]

Molecular weight: Translated: 29282; Mature: 29282

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLSRNSRVSIITKVLIENPNKIIGLNKFSDLLNAAKSTISEDIVIVREVLSKFSMGKV
CCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ETIAGAAGGIRYIPEVGEVEKRKFLEELCDIASESNRVVPGNFLYVTDIMFNPSIISKAS
HHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCHHHHHH
IILASYFQDMDIDYVITVETKGVPLAYEVAKCLGVQLITARRDSKVTEGSTVSINYVSGT
HHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEECCC
SGKIQQMCLSRKSLKQGSKCIFIDDFMRGGGTAKGLVDLLKEFDSELMGIGVLIDNKLST
CHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCC
NKITKDYVSLIDIVEINEDGVKMVPSM
CHHHHHHHHHHHHEEECCCCCEECCCC
>Mature Secondary Structure
MEKLSRNSRVSIITKVLIENPNKIIGLNKFSDLLNAAKSTISEDIVIVREVLSKFSMGKV
CCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ETIAGAAGGIRYIPEVGEVEKRKFLEELCDIASESNRVVPGNFLYVTDIMFNPSIISKAS
HHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCHHHHHH
IILASYFQDMDIDYVITVETKGVPLAYEVAKCLGVQLITARRDSKVTEGSTVSINYVSGT
HHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEECCC
SGKIQQMCLSRKSLKQGSKCIFIDDFMRGGGTAKGLVDLLKEFDSELMGIGVLIDNKLST
CHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCC
NKITKDYVSLIDIVEINEDGVKMVPSM
CHHHHHHHHHHHHEEECCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 560 [C]

Specific activity: 14

Km value (mM): 0.02 {adenine}} 0.125 {5-phospho-alpha-D-ribose} [C]

Substrates: Adenine; 5-Phospho-alpha-D-ribose 1-diphosphate [C]

Specific reaction: Adenine + 5-Phospho-alpha-D-ribose 1-diphosphate --> AMP + Diphosphate [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]