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The map label for this gene is prs

Identifier: 18311471

GI number: 18311471

Start: 2850143

End: 2851102

Strand: Reverse

Name: prs

Synonym: CPE2489

Alternate gene names: 18311471

Gene position: 2851102-2850143 (Counterclockwise)

Preceding gene: 18311472

Following gene: 18311470

Centisome position: 94.05

GC content: 32.08

Gene sequence:

>960_bases
ATGGAAAATCATTCAAAAAACATAAAAATATTTACAGGTAATTCTCATCCAGAATTAGCTAGAGAGATTGCAAAGGCGCT
AAACATTCCTCTAGGTAAAGCTGAAGTTGGTACTTTCAGTGATGGAGAAATATCAGTAAATATAAAAGAAACTGTTAGAG
GTTGCGATGTATTTATAGTTCAATCAACTTGTAGTCCTGTAAATAATAACTTAATGGAGTTATTAATAATGATAGATGCC
TTTAAAAGAGCATCAGCAGGAAGAATAAATGCGGTTATACCTTACTATGGATATGCTAGACAAGATAGAAAAGCTAAGTC
AAGAGATCCAATAACAGCTAAGTTAGTAGCTGACCTATTAACAGCAGCGGGTGCAGATAGAGTTCTTACTATGGATTTAC
ATGCAGCACAAATTCAAGGTTATTTTAATATACCAGTAGATCACTTATTAGGCTCACCAATTTTAGCAAAATATTTTGTT
GAAAAAGGATTAGCTGATAGAGATGATGTAGTTGTTGTTTCACCAGATTTAGGTTCAGTAACTAGAGCTAGAAAGTTTGC
AGATAAACTTAATGCTCCAATAGCTATAATTGATAAAAGAAGACCAAAAGCAAATGTATCTGAAATAATGAATATAATAG
GAGATGTTAAGGACAAAGTTTGTATCTTAATAGACGATATGATAGACACTGCAGGAACAATAACTAATGCAGCTAATGCT
CTTAAAGATTTAGGAGCAAAAAATGTATATGCTTGTTGTACTCATGGAGTATTATCAGGTCCTGCATTTGAAAGAATAAA
TAACAGTGCTATTGAAGAGTTAGTTATGTTAAATACTATAGCTCTTCCAGAGGGAGAAGGTTTAAATAAGTTTAAATCAT
TATCAGTTGCACCAATTATGGCAGATGCAATAAATAGAATTTATGATGATGAACCATTAAGTGGATTATTCCAAGACTAA

Upstream 100 bases:

>100_bases
AACAAAACATAGAGGGCTGGGTTGAAAGAAAAAAGCTTAAATAAGCATTAAATATACAAATGCCAAAGGCTAGGTTTAAT
TTTAAGGAGGTCTTCACTAA

Downstream 100 bases:

>100_bases
AAAAAAGAGCGCATATGTCGCTCTTTTTTTTATAAAATCACTAAAAATTGAAACTTTTTTTAAACAAAAACGTTTAAATT
TGATTAAAATGTAAAAATAA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MENHSKNIKIFTGNSHPELAREIAKALNIPLGKAEVGTFSDGEISVNIKETVRGCDVFIVQSTCSPVNNNLMELLIMIDA
FKRASAGRINAVIPYYGYARQDRKAKSRDPITAKLVADLLTAAGADRVLTMDLHAAQIQGYFNIPVDHLLGSPILAKYFV
EKGLADRDDVVVVSPDLGSVTRARKFADKLNAPIAIIDKRRPKANVSEIMNIIGDVKDKVCILIDDMIDTAGTITNAANA
LKDLGAKNVYACCTHGVLSGPAFERINNSAIEELVMLNTIALPEGEGLNKFKSLSVAPIMADAINRIYDDEPLSGLFQD

Sequences:

>Translated_319_residues
MENHSKNIKIFTGNSHPELAREIAKALNIPLGKAEVGTFSDGEISVNIKETVRGCDVFIVQSTCSPVNNNLMELLIMIDA
FKRASAGRINAVIPYYGYARQDRKAKSRDPITAKLVADLLTAAGADRVLTMDLHAAQIQGYFNIPVDHLLGSPILAKYFV
EKGLADRDDVVVVSPDLGSVTRARKFADKLNAPIAIIDKRRPKANVSEIMNIIGDVKDKVCILIDDMIDTAGTITNAANA
LKDLGAKNVYACCTHGVLSGPAFERINNSAIEELVMLNTIALPEGEGLNKFKSLSVAPIMADAINRIYDDEPLSGLFQD
>Mature_319_residues
MENHSKNIKIFTGNSHPELAREIAKALNIPLGKAEVGTFSDGEISVNIKETVRGCDVFIVQSTCSPVNNNLMELLIMIDA
FKRASAGRINAVIPYYGYARQDRKAKSRDPITAKLVADLLTAAGADRVLTMDLHAAQIQGYFNIPVDHLLGSPILAKYFV
EKGLADRDDVVVVSPDLGSVTRARKFADKLNAPIAIIDKRRPKANVSEIMNIIGDVKDKVCILIDDMIDTAGTITNAANA
LKDLGAKNVYACCTHGVLSGPAFERINNSAIEELVMLNTIALPEGEGLNKFKSLSVAPIMADAINRIYDDEPLSGLFQD

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=50.6369426751592, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI4506129, Length=314, Percent_Identity=49.6815286624204, Blast_Score=309, Evalue=3e-84,
Organism=Homo sapiens, GI84875539, Length=317, Percent_Identity=49.211356466877, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=49.3630573248408, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI4506133, Length=346, Percent_Identity=36.7052023121387, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI194018537, Length=347, Percent_Identity=36.3112391930836, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI310128524, Length=144, Percent_Identity=34.7222222222222, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI310115209, Length=144, Percent_Identity=34.7222222222222, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI310118259, Length=144, Percent_Identity=34.7222222222222, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI310119946, Length=144, Percent_Identity=34.7222222222222, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=55.5555555555556, Blast_Score=349, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=48.7261146496815, Blast_Score=300, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=48.7261146496815, Blast_Score=300, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=48.7261146496815, Blast_Score=299, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17554704, Length=311, Percent_Identity=48.2315112540193, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17570245, Length=343, Percent_Identity=34.402332361516, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319403, Length=313, Percent_Identity=47.6038338658147, Blast_Score=276, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6320946, Length=315, Percent_Identity=47.6190476190476, Blast_Score=276, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6321776, Length=317, Percent_Identity=45.1104100946372, Blast_Score=265, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6322667, Length=200, Percent_Identity=43.5, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6324511, Length=95, Percent_Identity=50.5263157894737, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21355239, Length=321, Percent_Identity=49.8442367601246, Blast_Score=305, Evalue=3e-83,
Organism=Drosophila melanogaster, GI45551540, Length=344, Percent_Identity=46.5116279069767, Blast_Score=291, Evalue=3e-79,
Organism=Drosophila melanogaster, GI281362873, Length=364, Percent_Identity=33.2417582417582, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24651454, Length=364, Percent_Identity=33.2417582417582, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24651458, Length=364, Percent_Identity=33.2417582417582, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24651456, Length=364, Percent_Identity=33.2417582417582, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24651462, Length=210, Percent_Identity=38.5714285714286, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24651464, Length=210, Percent_Identity=38.5714285714286, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI45552010, Length=210, Percent_Identity=38.5714285714286, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS_CLOPE (Q8XHJ4)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_563405.1
- ProteinModelPortal:   Q8XHJ4
- SMR:   Q8XHJ4
- GeneID:   990867
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE2489
- NMPDR:   fig|195102.1.peg.2552
- HOGENOM:   HBG519284
- OMA:   CATHAVF
- PhylomeDB:   Q8XHJ4
- ProtClustDB:   PRK01259
- BioCyc:   CPER195102:CPE2489-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 34579; Mature: 34579

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENHSKNIKIFTGNSHPELAREIAKALNIPLGKAEVGTFSDGEISVNIKETVRGCDVFIV
CCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHCCCEEEEE
QSTCSPVNNNLMELLIMIDAFKRASAGRINAVIPYYGYARQDRKAKSRDPITAKLVADLL
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHCCCCCCCCHHHHHHHHHH
TAAGADRVLTMDLHAAQIQGYFNIPVDHLLGSPILAKYFVEKGLADRDDVVVVSPDLGSV
HHCCCCCEEEEEEHHHHHCCEECCCHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCHH
TRARKFADKLNAPIAIIDKRRPKANVSEIMNIIGDVKDKVCILIDDMIDTAGTITNAANA
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHH
LKDLGAKNVYACCTHGVLSGPAFERINNSAIEELVMLNTIALPEGEGLNKFKSLSVAPIM
HHHCCCCCHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHH
ADAINRIYDDEPLSGLFQD
HHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MENHSKNIKIFTGNSHPELAREIAKALNIPLGKAEVGTFSDGEISVNIKETVRGCDVFIV
CCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHCCCEEEEE
QSTCSPVNNNLMELLIMIDAFKRASAGRINAVIPYYGYARQDRKAKSRDPITAKLVADLL
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHCCCCCCCCHHHHHHHHHH
TAAGADRVLTMDLHAAQIQGYFNIPVDHLLGSPILAKYFVEKGLADRDDVVVVSPDLGSV
HHCCCCCEEEEEEHHHHHCCEECCCHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCHH
TRARKFADKLNAPIAIIDKRRPKANVSEIMNIIGDVKDKVCILIDDMIDTAGTITNAANA
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHH
LKDLGAKNVYACCTHGVLSGPAFERINNSAIEELVMLNTIALPEGEGLNKFKSLSVAPIM
HHHCCCCCHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHH
ADAINRIYDDEPLSGLFQD
HHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842