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The map label for this gene is bioB [H]

Identifier: 18311442

GI number: 18311442

Start: 2813812

End: 2814855

Strand: Direct

Name: bioB [H]

Synonym: CPE2460

Alternate gene names: 18311442

Gene position: 2813812-2814855 (Clockwise)

Preceding gene: 18311440

Following gene: 161485639

Centisome position: 92.82

GC content: 31.8

Gene sequence:

>1044_bases
ATGAATACTATTATACAAAAAGCAAAAGAAACTCATGAACTTTCAAGAGATGAAATTATAGCCTTATTAAAAGATGATTC
TATAAATGAAGAACTTTTTAAAGCTGCTGATGAAGTTAGAAAAAAATACCTTGGTGATGAAGTACATTTAAGAGGACTTA
TAGAATTCACTAATATATGTAAAAGAAACTGTATGTATTGTGGTCTTAGAAGAGATAATAAAAATCTTAATAGATATAGA
CTTTCTCACGAAGAGATTATAGATTTTGCAAAAAAAGCCGTTGGTTATGGATATAAAACCTTAGTTCTTCAAGGCGGTGA
AGATGATTATTATACTGTTGAACGTTTGGTTCCTATAGTTAAAGATCTTAAAGCCTTAGGAGTTGCCTTAACATTAAGTA
TAGGCGAAAGACCTTTTGAAGAATATGAAGCCTTAAAAAAAGCTGGAGCTGATAGATTCCTTTTAAGAATTGAAACAACA
GATAGAGAATTATATGAAGAACTTGATCCAGGTATGAGTCATGAAAATAGAATTCAATGTCTTAAAAACTTAAGAAAACT
TGGATATGAAGTAGGAAGTGGATGTCTTGTTGGTCTTCCTGGTCAAAAAATAGAATCCTTAGCTGATGATATTTTATTTT
TCAAAGAACTTGATGTAGATATGAATGGTATAGGCCCATTTATTCCAAATGAAGATACTCCACTAAAAGATGCTGAAGGT
GGACAATTTGAACTAGCCCTAAAAGTAATGGCTATAGTTAGATTACTTTTACCTGATATAAATATTCCTGCTACAACAGC
TATGGAAACATTAAATAAACAAGGAAGAGTAATAGCTCTTCAATGCGGAGCAAATGTAGTAATGCCTAACGTTACTGAAG
GTGAATATAGAAAACTTTATGCCTTATATCCAGGTAAAATATGTACAGGCGATACACCTGCTCATTGTAGAGGATGTATC
TCTGGAAAAATTAGAGGTATAGGTAGAATAGTTTCTGACGGACCTGGATTTAGAGCTAATGGATTTAAACCTAAAACTCG
TTAA

Upstream 100 bases:

>100_bases
CTTGTTAAATTATAATTATTAATTAATAATTATTTTAGGATAAAAAGAGTAATTTTTAATATCTAAAATCCTTATACCCA
GATTAATTAGGAGGATTAAT

Downstream 100 bases:

>100_bases
CAAAATATAATATAAATAATATTTATAAAACTCTAGAACAATTTAGTAAAATCTAAATCAAATTCTAGAGTTTTTTATTT
AGAGTTAAATAATAATTTCT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNTIIQKAKETHELSRDEIIALLKDDSINEELFKAADEVRKKYLGDEVHLRGLIEFTNICKRNCMYCGLRRDNKNLNRYR
LSHEEIIDFAKKAVGYGYKTLVLQGGEDDYYTVERLVPIVKDLKALGVALTLSIGERPFEEYEALKKAGADRFLLRIETT
DRELYEELDPGMSHENRIQCLKNLRKLGYEVGSGCLVGLPGQKIESLADDILFFKELDVDMNGIGPFIPNEDTPLKDAEG
GQFELALKVMAIVRLLLPDINIPATTAMETLNKQGRVIALQCGANVVMPNVTEGEYRKLYALYPGKICTGDTPAHCRGCI
SGKIRGIGRIVSDGPGFRANGFKPKTR

Sequences:

>Translated_347_residues
MNTIIQKAKETHELSRDEIIALLKDDSINEELFKAADEVRKKYLGDEVHLRGLIEFTNICKRNCMYCGLRRDNKNLNRYR
LSHEEIIDFAKKAVGYGYKTLVLQGGEDDYYTVERLVPIVKDLKALGVALTLSIGERPFEEYEALKKAGADRFLLRIETT
DRELYEELDPGMSHENRIQCLKNLRKLGYEVGSGCLVGLPGQKIESLADDILFFKELDVDMNGIGPFIPNEDTPLKDAEG
GQFELALKVMAIVRLLLPDINIPATTAMETLNKQGRVIALQCGANVVMPNVTEGEYRKLYALYPGKICTGDTPAHCRGCI
SGKIRGIGRIVSDGPGFRANGFKPKTR
>Mature_347_residues
MNTIIQKAKETHELSRDEIIALLKDDSINEELFKAADEVRKKYLGDEVHLRGLIEFTNICKRNCMYCGLRRDNKNLNRYR
LSHEEIIDFAKKAVGYGYKTLVLQGGEDDYYTVERLVPIVKDLKALGVALTLSIGERPFEEYEALKKAGADRFLLRIETT
DRELYEELDPGMSHENRIQCLKNLRKLGYEVGSGCLVGLPGQKIESLADDILFFKELDVDMNGIGPFIPNEDTPLKDAEG
GQFELALKVMAIVRLLLPDINIPATTAMETLNKQGRVIALQCGANVVMPNVTEGEYRKLYALYPGKICTGDTPAHCRGCI
SGKIRGIGRIVSDGPGFRANGFKPKTR

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=266, Percent_Identity=23.6842105263158, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 38875; Mature: 38875

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIIQKAKETHELSRDEIIALLKDDSINEELFKAADEVRKKYLGDEVHLRGLIEFTNIC
CCHHHHHHHHHHHCCHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KRNCMYCGLRRDNKNLNRYRLSHEEIIDFAKKAVGYGYKTLVLQGGEDDYYTVERLVPIV
HHCCEEEECCCCCCCCHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
KDLKALGVALTLSIGERPFEEYEALKKAGADRFLLRIETTDRELYEELDPGMSHENRIQC
HHHHHHCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHCCCCCCHHHHHHH
LKNLRKLGYEVGSGCLVGLPGQKIESLADDILFFKELDVDMNGIGPFIPNEDTPLKDAEG
HHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
GQFELALKVMAIVRLLLPDINIPATTAMETLNKQGRVIALQCGANVVMPNVTEGEYRKLY
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCEECCCCCCCCCEEEE
ALYPGKICTGDTPAHCRGCISGKIRGIGRIVSDGPGFRANGFKPKTR
EECCCEEECCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNTIIQKAKETHELSRDEIIALLKDDSINEELFKAADEVRKKYLGDEVHLRGLIEFTNIC
CCHHHHHHHHHHHCCHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KRNCMYCGLRRDNKNLNRYRLSHEEIIDFAKKAVGYGYKTLVLQGGEDDYYTVERLVPIV
HHCCEEEECCCCCCCCHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
KDLKALGVALTLSIGERPFEEYEALKKAGADRFLLRIETTDRELYEELDPGMSHENRIQC
HHHHHHCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHCCCCCCHHHHHHH
LKNLRKLGYEVGSGCLVGLPGQKIESLADDILFFKELDVDMNGIGPFIPNEDTPLKDAEG
HHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
GQFELALKVMAIVRLLLPDINIPATTAMETLNKQGRVIALQCGANVVMPNVTEGEYRKLY
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCEECCCCCCCCCEEEE
ALYPGKICTGDTPAHCRGCISGKIRGIGRIVSDGPGFRANGFKPKTR
EECCCEEECCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA