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The map label for this gene is murD [H]

Identifier: 18311441

GI number: 18311441

Start: 2812125

End: 2813501

Strand: Reverse

Name: murD [H]

Synonym: CPE2459

Alternate gene names: 18311441

Gene position: 2813501-2812125 (Counterclockwise)

Preceding gene: 18311443

Following gene: 18311438

Centisome position: 92.81

GC content: 28.83

Gene sequence:

>1377_bases
ATGAAAAGAGACTTTAATGAGTTTAAAGAATTTATAAAGGGAAAAAATGTAGCAGTTGTAGGAATTGGGGTAAGTAACAT
ACCTCTTATAAAGTTTTTAGTTAAACTAGGAGCTAAAGTAACTGCTTTTGATATGAAATCAGCTGAGGAGTTAGGTGAAA
TTTCAAAAGAATTTGAAATATTAGGAGTAAATTTAGAACTTGGAAAAGGTTATTTAGATAGATTAACTGGATTTGAGGTT
GTATTTAAAACACCATCTATGAGAATAGACAGTGAAGCTTTATTAAGATGTAAAAAGCAAGGTGCATATATTACTTCAGA
GATGGAAGAATTCGTAAGATATTGTAAAGGTAGAGTTTACGGGGTAACTGGTAGTGATGGTAAAACAACAACTACAACAA
TAGTGTCTAAGTTATTATCACAAGAGGGATATAAAACTTGGACAGGAGGAAATATAGGTACTCCATTATTCTCAAATATA
GAAGAGATAAAAGAAGAAGATAAAGTTGTATTAGAATTATCAAGTTTTCAGCTTATGACTATGGATGTTGAAATTGATGT
AGCAATAGTTACTAATATAACTCCAAATCACTTAGATATGCATAAAGATATGCAAGAATATATTGATGCTAAGAAAAATG
TTTTCAAATATCAAAGAGAAAATGATCTTTTAGTAATTAATGATGAAAATGAAATAACTAAAAATTTAGATAAAGAAGCA
AAAGGAAAAGTTGTTAGATTTAGTTCAAAGAAAACAGAAGGAGAAGATGCATACTATAAAGATGGAAAGTTATATGTACA
TGGTAAAGAAGTATGTAAAAAAGATAATATAATCATAAAGGGAATGCATAATGTAGAAAATTATTTAGCTGCATTCTTAG
CTGTTTATGATGAAGTATCAATAGAATCAATGAAAAAGGTAGCAGAAACTTTTGGAGGAGTTCATCATAGATGTGAGTTC
ATAAGAGAAGTTGATGGAGTAAAATATTATAATGATTCTATAGCATCAACTCCAACAAGAACTTTAGCTGGATTAAAAGC
TTTTGAAAAACCAGTTATACTTTTAGCAGGAGGATATGATAAACATGTACCTTTTGAGCCTTTAGCTTATGAAGGATATG
AAAAAATAAAAGCTATAGTATTATTTGGAGTAACTAAAGAAAAAATAAAAGCAGCTTTCAAGAGATTAGAAGAAGAAAAA
GGAATTCATGTACCAGTCTATTCAGGAGAAAGCTTAGAAGAGGTAGTAAATATAGCTAAATCAATAGCTGAAAGTGGAGA
TATAATAACACTTTCACCTGCTTGCGCAAGTTTTGATATGTTTAAAAACTTTGAAGTAAGAGGAGATAAGTTTAAGGAAA
TAGTAAATAATATATAA

Upstream 100 bases:

>100_bases
TATGGAATGGAAAATTCCATAGTGTTATAATTAGAAAGAAATGGGATACAATTTATAAAGAACCATTATTAATATTGGTA
AAAATTTTGGAGGCCACAAA

Downstream 100 bases:

>100_bases
TGAGATAAATATATCTTAAAATGAGCCATATTATGATTTTAGTTTAATTAATTCTTTGAAATTATTAAAGCTAAAAAAAT
AATATGGCTTATTTTTATTT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKRDFNEFKEFIKGKNVAVVGIGVSNIPLIKFLVKLGAKVTAFDMKSAEELGEISKEFEILGVNLELGKGYLDRLTGFEV
VFKTPSMRIDSEALLRCKKQGAYITSEMEEFVRYCKGRVYGVTGSDGKTTTTTIVSKLLSQEGYKTWTGGNIGTPLFSNI
EEIKEEDKVVLELSSFQLMTMDVEIDVAIVTNITPNHLDMHKDMQEYIDAKKNVFKYQRENDLLVINDENEITKNLDKEA
KGKVVRFSSKKTEGEDAYYKDGKLYVHGKEVCKKDNIIIKGMHNVENYLAAFLAVYDEVSIESMKKVAETFGGVHHRCEF
IREVDGVKYYNDSIASTPTRTLAGLKAFEKPVILLAGGYDKHVPFEPLAYEGYEKIKAIVLFGVTKEKIKAAFKRLEEEK
GIHVPVYSGESLEEVVNIAKSIAESGDIITLSPACASFDMFKNFEVRGDKFKEIVNNI

Sequences:

>Translated_458_residues
MKRDFNEFKEFIKGKNVAVVGIGVSNIPLIKFLVKLGAKVTAFDMKSAEELGEISKEFEILGVNLELGKGYLDRLTGFEV
VFKTPSMRIDSEALLRCKKQGAYITSEMEEFVRYCKGRVYGVTGSDGKTTTTTIVSKLLSQEGYKTWTGGNIGTPLFSNI
EEIKEEDKVVLELSSFQLMTMDVEIDVAIVTNITPNHLDMHKDMQEYIDAKKNVFKYQRENDLLVINDENEITKNLDKEA
KGKVVRFSSKKTEGEDAYYKDGKLYVHGKEVCKKDNIIIKGMHNVENYLAAFLAVYDEVSIESMKKVAETFGGVHHRCEF
IREVDGVKYYNDSIASTPTRTLAGLKAFEKPVILLAGGYDKHVPFEPLAYEGYEKIKAIVLFGVTKEKIKAAFKRLEEEK
GIHVPVYSGESLEEVVNIAKSIAESGDIITLSPACASFDMFKNFEVRGDKFKEIVNNI
>Mature_458_residues
MKRDFNEFKEFIKGKNVAVVGIGVSNIPLIKFLVKLGAKVTAFDMKSAEELGEISKEFEILGVNLELGKGYLDRLTGFEV
VFKTPSMRIDSEALLRCKKQGAYITSEMEEFVRYCKGRVYGVTGSDGKTTTTTIVSKLLSQEGYKTWTGGNIGTPLFSNI
EEIKEEDKVVLELSSFQLMTMDVEIDVAIVTNITPNHLDMHKDMQEYIDAKKNVFKYQRENDLLVINDENEITKNLDKEA
KGKVVRFSSKKTEGEDAYYKDGKLYVHGKEVCKKDNIIIKGMHNVENYLAAFLAVYDEVSIESMKKVAETFGGVHHRCEF
IREVDGVKYYNDSIASTPTRTLAGLKAFEKPVILLAGGYDKHVPFEPLAYEGYEKIKAIVLFGVTKEKIKAAFKRLEEEK
GIHVPVYSGESLEEVVNIAKSIAESGDIITLSPACASFDMFKNFEVRGDKFKEIVNNI

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=452, Percent_Identity=27.8761061946903, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 51514; Mature: 51514

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRDFNEFKEFIKGKNVAVVGIGVSNIPLIKFLVKLGAKVTAFDMKSAEELGEISKEFEI
CCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCEEEEECHHHHHHHHHHHHCEEE
LGVNLELGKGYLDRLTGFEVVFKTPSMRIDSEALLRCKKQGAYITSEMEEFVRYCKGRVY
EEEEEECCCCHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEEE
GVTGSDGKTTTTTIVSKLLSQEGYKTWTGGNIGTPLFSNIEEIKEEDKVVLELSSFQLMT
EEECCCCCHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHCCCEEEEEEECEEEEE
MDVEIDVAIVTNITPNHLDMHKDMQEYIDAKKNVFKYQRENDLLVINDENEITKNLDKEA
EEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHH
KGKVVRFSSKKTEGEDAYYKDGKLYVHGKEVCKKDNIIIKGMHNVENYLAAFLAVYDEVS
CCCEEEEECCCCCCCCCEEECCEEEEECHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHH
IESMKKVAETFGGVHHRCEFIREVDGVKYYNDSIASTPTRTLAGLKAFEKPVILLAGGYD
HHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCC
KHVPFEPLAYEGYEKIKAIVLFGVTKEKIKAAFKRLEEEKGIHVPVYSGESLEEVVNIAK
CCCCCCCCCCCCHHHEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
SIAESGDIITLSPACASFDMFKNFEVRGDKFKEIVNNI
HHHCCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKRDFNEFKEFIKGKNVAVVGIGVSNIPLIKFLVKLGAKVTAFDMKSAEELGEISKEFEI
CCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCEEEEECHHHHHHHHHHHHCEEE
LGVNLELGKGYLDRLTGFEVVFKTPSMRIDSEALLRCKKQGAYITSEMEEFVRYCKGRVY
EEEEEECCCCHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEEE
GVTGSDGKTTTTTIVSKLLSQEGYKTWTGGNIGTPLFSNIEEIKEEDKVVLELSSFQLMT
EEECCCCCHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHCCCEEEEEEECEEEEE
MDVEIDVAIVTNITPNHLDMHKDMQEYIDAKKNVFKYQRENDLLVINDENEITKNLDKEA
EEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHH
KGKVVRFSSKKTEGEDAYYKDGKLYVHGKEVCKKDNIIIKGMHNVENYLAAFLAVYDEVS
CCCEEEEECCCCCCCCCEEECCEEEEECHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHH
IESMKKVAETFGGVHHRCEFIREVDGVKYYNDSIASTPTRTLAGLKAFEKPVILLAGGYD
HHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCC
KHVPFEPLAYEGYEKIKAIVLFGVTKEKIKAAFKRLEEEKGIHVPVYSGESLEEVVNIAK
CCCCCCCCCCCCHHHEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
SIAESGDIITLSPACASFDMFKNFEVRGDKFKEIVNNI
HHHCCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA