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The map label for this gene is gltD [H]

Identifier: 18311443

GI number: 18311443

Start: 2815098

End: 2816339

Strand: Reverse

Name: gltD [H]

Synonym: CPE2461

Alternate gene names: 18311443

Gene position: 2816339-2815098 (Counterclockwise)

Preceding gene: 18311444

Following gene: 18311441

Centisome position: 92.9

GC content: 31.88

Gene sequence:

>1242_bases
ATGACAGTAAATGAGCAAAACTTAAAAGAAGAAGCTTTAAGATGCTTAATGTGCAAAAATCCAAGATGTAAGGCTAATTG
CCCAATAAGTACACCTATTCCAGAGGTAATAGGATTATTTAAAGAAGATAAAATAAAAGAGGCTGGGGAAATTTTATTTG
AAAACAATCCTTTATCAGTAGTTTGTTCCATAGTATGTCCTCATGAAAATCAATGTAAGGGGAATTGTATAAAAGGAATA
AAGGATACACCAGTAAACTTTAGTGAAATAGAGAGAGAAATATCAGAAGAGTTTTTAGATAATCTAGAACTTACTCAAGA
TACTATTTTAGAAGATAAAATTGCTATAATAGGTGGAGGTCCTGCGGGAATAACAGCTGCATTTATATTAGCTAAAAAGG
GATATCAAGTAACAATATTTGATGAAAATGATAAAATTGGTGGAGTATTAAGATATGGAATACCAGAGTTTAGACTTTCT
AGAGATATAATAGATAAATATGAAGAGGCCCTTATTAAATTAAATGTTAAAATAAGACCTAACACTTTAATAGGAACAGT
TATAACTTTAGATATGTTAAAAAGTGATGGTTATAAAGCAATATTTATAGCAACTGGGGTTTGGAATCCTAAGACTTTAA
ATGTTAAGGGAGAAACTTTAGGCAATGTTCACTATGCTATAGATTATTTAAAAGCACCAAAATCATATAATCTTGGAGAG
AGAGTAGTTGTAATAGGAGCTGGAAATGTTGCAATGGATGCTGCTAGAACTGCAAAGAGAAATGGTGCAGATACATATGT
GCTTTATAGAAAAAGCTTTGAAGATATGCCAGCTACTAAGGTTGAAATAGAAGAGGCTAAGGAAGATGGCGTAAAATTTG
AACTTTATAAAGCTCCTTTAGAGATTTATGAGGATGGAGTAAAATACATACAAACAGAAAGAGTAATTTCTGAGGATGGA
AGAGTATCATTTAAAACCTTAGAAGGTAGTGAAGGTGTGTTTAAATGCGATTCAATAATAGTTGCCATAAGCCAAGCGCC
TAGAACTAATATAGTATCTAGTAGTAGAGATCTTTCAACAGATAAATATGGCTTAGTAGTAACTGACGAAAAGGGAATAA
CAACACTTAATGGAGTTTTTGCTTCAGGAGATGTTGTAAGTGGTCCTAAGACTGTAGTTGAGGCAGTTGCAAATAGTAAA
AAGGTTGCTCATGCTATAGATGAATTTTGTAAAAACAATTAA

Upstream 100 bases:

>100_bases
CTTTAAATAAATGACTTTATTAAATTATTTTGATAATATAATATATTATGAAACAATGAAGAATTTTATAGGTTGTTAAG
AAATCAGGGGGTTTATTAAA

Downstream 100 bases:

>100_bases
AAAGTAATTAAGTTTAATATTGAAATTAAATAATTTATAAGACTGTAGGGAAGTTTATATAAAAATAAATTTCCCTACAG
TTTTTTAGGTAATTAAAAGA

Product: putative oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTVNEQNLKEEALRCLMCKNPRCKANCPISTPIPEVIGLFKEDKIKEAGEILFENNPLSVVCSIVCPHENQCKGNCIKGI
KDTPVNFSEIEREISEEFLDNLELTQDTILEDKIAIIGGGPAGITAAFILAKKGYQVTIFDENDKIGGVLRYGIPEFRLS
RDIIDKYEEALIKLNVKIRPNTLIGTVITLDMLKSDGYKAIFIATGVWNPKTLNVKGETLGNVHYAIDYLKAPKSYNLGE
RVVVIGAGNVAMDAARTAKRNGADTYVLYRKSFEDMPATKVEIEEAKEDGVKFELYKAPLEIYEDGVKYIQTERVISEDG
RVSFKTLEGSEGVFKCDSIIVAISQAPRTNIVSSSRDLSTDKYGLVVTDEKGITTLNGVFASGDVVSGPKTVVEAVANSK
KVAHAIDEFCKNN

Sequences:

>Translated_413_residues
MTVNEQNLKEEALRCLMCKNPRCKANCPISTPIPEVIGLFKEDKIKEAGEILFENNPLSVVCSIVCPHENQCKGNCIKGI
KDTPVNFSEIEREISEEFLDNLELTQDTILEDKIAIIGGGPAGITAAFILAKKGYQVTIFDENDKIGGVLRYGIPEFRLS
RDIIDKYEEALIKLNVKIRPNTLIGTVITLDMLKSDGYKAIFIATGVWNPKTLNVKGETLGNVHYAIDYLKAPKSYNLGE
RVVVIGAGNVAMDAARTAKRNGADTYVLYRKSFEDMPATKVEIEEAKEDGVKFELYKAPLEIYEDGVKYIQTERVISEDG
RVSFKTLEGSEGVFKCDSIIVAISQAPRTNIVSSSRDLSTDKYGLVVTDEKGITTLNGVFASGDVVSGPKTVVEAVANSK
KVAHAIDEFCKNN
>Mature_412_residues
TVNEQNLKEEALRCLMCKNPRCKANCPISTPIPEVIGLFKEDKIKEAGEILFENNPLSVVCSIVCPHENQCKGNCIKGIK
DTPVNFSEIEREISEEFLDNLELTQDTILEDKIAIIGGGPAGITAAFILAKKGYQVTIFDENDKIGGVLRYGIPEFRLSR
DIIDKYEEALIKLNVKIRPNTLIGTVITLDMLKSDGYKAIFIATGVWNPKTLNVKGETLGNVHYAIDYLKAPKSYNLGER
VVVIGAGNVAMDAARTAKRNGADTYVLYRKSFEDMPATKVEIEEAKEDGVKFELYKAPLEIYEDGVKYIQTERVISEDGR
VSFKTLEGSEGVFKCDSIIVAISQAPRTNIVSSSRDLSTDKYGLVVTDEKGITTLNGVFASGDVVSGPKTVVEAVANSKK
VAHAIDEFCKNN

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=445, Percent_Identity=29.438202247191, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1789606, Length=432, Percent_Identity=31.712962962963, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788811, Length=432, Percent_Identity=31.712962962963, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI87082180, Length=433, Percent_Identity=32.5635103926097, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1788468, Length=414, Percent_Identity=31.4009661835749, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1789243, Length=402, Percent_Identity=31.3432835820896, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71984108, Length=444, Percent_Identity=28.6036036036036, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17570289, Length=448, Percent_Identity=26.7857142857143, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17543860, Length=156, Percent_Identity=32.0512820512821, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=457, Percent_Identity=26.9146608315098, Blast_Score=145, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24665547, Length=444, Percent_Identity=26.8018018018018, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24665543, Length=444, Percent_Identity=26.8018018018018, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI28574881, Length=444, Percent_Identity=26.8018018018018, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24665539, Length=444, Percent_Identity=26.8018018018018, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24640763, Length=440, Percent_Identity=27.9545454545455, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI18858217, Length=440, Percent_Identity=27.9545454545455, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 45421; Mature: 45289

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01108 RIBOSOMAL_L24

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVNEQNLKEEALRCLMCKNPRCKANCPISTPIPEVIGLFKEDKIKEAGEILFENNPLSV
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCEE
VCSIVCPHENQCKGNCIKGIKDTPVNFSEIEREISEEFLDNLELTQDTILEDKIAIIGGG
EEEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECC
PAGITAAFILAKKGYQVTIFDENDKIGGVLRYGIPEFRLSRDIIDKYEEALIKLNVKIRP
CHHHHHHHHEECCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHEEEEEEEEECC
NTLIGTVITLDMLKSDGYKAIFIATGVWNPKTLNVKGETLGNVHYAIDYLKAPKSYNLGE
CCCEEHHEEEEHHHCCCCEEEEEEECCCCCEEEEECCCCCCCHHEEEHHHHCCCCCCCCC
RVVVIGAGNVAMDAARTAKRNGADTYVLYRKSFEDMPATKVEIEEAKEDGVKFELYKAPL
EEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEHHHHHHCCCEEEEECCCH
EIYEDGVKYIQTERVISEDGRVSFKTLEGSEGVFKCDSIIVAISQAPRTNIVSSSRDLST
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECEEEEEECCCCCCCCCCCCCCCCC
DKYGLVVTDEKGITTLNGVFASGDVVSGPKTVVEAVANSKKVAHAIDEFCKNN
CCEEEEEECCCCCEEECCEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVNEQNLKEEALRCLMCKNPRCKANCPISTPIPEVIGLFKEDKIKEAGEILFENNPLSV
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCEE
VCSIVCPHENQCKGNCIKGIKDTPVNFSEIEREISEEFLDNLELTQDTILEDKIAIIGGG
EEEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECC
PAGITAAFILAKKGYQVTIFDENDKIGGVLRYGIPEFRLSRDIIDKYEEALIKLNVKIRP
CHHHHHHHHEECCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHEEEEEEEEECC
NTLIGTVITLDMLKSDGYKAIFIATGVWNPKTLNVKGETLGNVHYAIDYLKAPKSYNLGE
CCCEEHHEEEEHHHCCCCEEEEEEECCCCCEEEEECCCCCCCHHEEEHHHHCCCCCCCCC
RVVVIGAGNVAMDAARTAKRNGADTYVLYRKSFEDMPATKVEIEEAKEDGVKFELYKAPL
EEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEHHHHHHCCCEEEEECCCH
EIYEDGVKYIQTERVISEDGRVSFKTLEGSEGVFKCDSIIVAISQAPRTNIVSSSRDLST
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECEEEEEECCCCCCCCCCCCCCCCC
DKYGLVVTDEKGITTLNGVFASGDVVSGPKTVVEAVANSKKVAHAIDEFCKNN
CCEEEEEECCCCCEEECCEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]