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The map label for this gene is trxB [C]

Identifier: 18311422

GI number: 18311422

Start: 2787053

End: 2787991

Strand: Reverse

Name: trxB [C]

Synonym: CPE2440

Alternate gene names: 18311422

Gene position: 2787991-2787053 (Counterclockwise)

Preceding gene: 18311423

Following gene: 18311421

Centisome position: 91.97

GC content: 32.48

Gene sequence:

>939_bases
ATGGATAAAGAAATAAAAGAATTAGATTTAATAATAATTGGAGCAGGTCCAGCAGGATTAACATCTGCAATATATGCTTC
AAGAGCAAAATTAAGTACTTTAGTTTTAGAAGATAATTTAGTTGGAGGTCAAGTAAGATCAACTTATACAGTTGAAAACT
ATCCTGGATTTACAGAAATAACAGGAAATGATCTTGCTGATAGAATCCAAGCGCAAGCAGAGGCTTGTGGAGCTATAATA
GATGAATTTGATTTTATAGAAAATGTCTCATTAAAAGATGATGAAAAAATCATAGAGACTGGAGATTATATATATAAGCC
AAAGGCTGTAATAATAGCTACAGGAGCTACACCTAAGAAATTACCAATTCCAAGTGAAAGTAAATATCTAGGTAAGGGTG
TGCATTACTGTGCAGTATGTGATGGAGCAGTTTACCAAGATGAGGTTGTTGCCGTTGTAGGTGGCGGAAATGCTGCTTTA
GAAGAAGCTTTATATCTATCTAATATAGTTAAGAAAGTAATTGTTATAAGAAGATATGATTATTTCCGTGCAGAAGCTAA
GACATTAGAGGCTGCAAGTAATAAGGAAAATATTGAAATAATGTATAACTGGGATTTAGTTGATGTTCTAGGTGGAGAGT
TTGTTGAAGCTGCAAGAATAAAAAATACTAAAACTGGAGAAGAAAAAGAAATTGCTATAAATGGAGTCTTTGGATATATA
GGAACAGAACCTAAGACATCTATGTTTAGAGAATATATAAATGTTAAAGAAAATGGTTATATTGAAGGCGATGAAAACAT
GAGAACTAATGTTAAGGGTGTTTATGTAGCGGGAGATGTTAGAGAAAAGATGTTTAGACAAATAACTACTGCCGTTTCAG
ATGGAACTATAGCAGCCTTACATGCAGAAAAATATATTTCAGAAATAAAGGAGAGATAA

Upstream 100 bases:

>100_bases
AGGAGTATAATTAATAAAAAATAGGTACCCTAAGGGGGTACATCGAATAATATATCATATAATACAAATAATAAATTTAA
CCTCAGTAAGGAGTGTTAGT

Downstream 100 bases:

>100_bases
GTATGGTAAAAGTATATTCAGTTGAATGGTGTGGACCATGTAATAAATTAAAAAAATATTTAGAAAAATTAGGCGTTCAA
TACGAAACAATCACAGTTGC

Product: thioredoxin-disulfide reductase

Products: thioredoxin disulfide; NADPH; H+

Alternate protein names: ORF A [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MDKEIKELDLIIIGAGPAGLTSAIYASRAKLSTLVLEDNLVGGQVRSTYTVENYPGFTEITGNDLADRIQAQAEACGAII
DEFDFIENVSLKDDEKIIETGDYIYKPKAVIIATGATPKKLPIPSESKYLGKGVHYCAVCDGAVYQDEVVAVVGGGNAAL
EEALYLSNIVKKVIVIRRYDYFRAEAKTLEAASNKENIEIMYNWDLVDVLGGEFVEAARIKNTKTGEEKEIAINGVFGYI
GTEPKTSMFREYINVKENGYIEGDENMRTNVKGVYVAGDVREKMFRQITTAVSDGTIAALHAEKYISEIKER

Sequences:

>Translated_312_residues
MDKEIKELDLIIIGAGPAGLTSAIYASRAKLSTLVLEDNLVGGQVRSTYTVENYPGFTEITGNDLADRIQAQAEACGAII
DEFDFIENVSLKDDEKIIETGDYIYKPKAVIIATGATPKKLPIPSESKYLGKGVHYCAVCDGAVYQDEVVAVVGGGNAAL
EEALYLSNIVKKVIVIRRYDYFRAEAKTLEAASNKENIEIMYNWDLVDVLGGEFVEAARIKNTKTGEEKEIAINGVFGYI
GTEPKTSMFREYINVKENGYIEGDENMRTNVKGVYVAGDVREKMFRQITTAVSDGTIAALHAEKYISEIKER
>Mature_312_residues
MDKEIKELDLIIIGAGPAGLTSAIYASRAKLSTLVLEDNLVGGQVRSTYTVENYPGFTEITGNDLADRIQAQAEACGAII
DEFDFIENVSLKDDEKIIETGDYIYKPKAVIIATGATPKKLPIPSESKYLGKGVHYCAVCDGAVYQDEVVAVVGGGNAAL
EEALYLSNIVKKVIVIRRYDYFRAEAKTLEAASNKENIEIMYNWDLVDVLGGEFVEAARIKNTKTGEEKEIAINGVFGYI
GTEPKTSMFREYINVKENGYIEGDENMRTNVKGVYVAGDVREKMFRQITTAVSDGTIAALHAEKYISEIKER

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787114, Length=317, Percent_Identity=38.4858044164038, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI87081763, Length=315, Percent_Identity=30.4761904761905, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320560, Length=316, Percent_Identity=37.3417721518987, Blast_Score=194, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321898, Length=318, Percent_Identity=35.2201257861635, Blast_Score=186, Evalue=3e-48,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9

Molecular weight: Translated: 34312; Mature: 34312

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKEIKELDLIIIGAGPAGLTSAIYASRAKLSTLVLEDNLVGGQVRSTYTVENYPGFTEI
CCCCCCEEEEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEECCCCCCEEE
TGNDLADRIQAQAEACGAIIDEFDFIENVSLKDDEKIIETGDYIYKPKAVIIATGATPKK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCEEEEECCCCCCC
LPIPSESKYLGKGVHYCAVCDGAVYQDEVVAVVGGGNAALEEALYLSNIVKKVIVIRRYD
CCCCCCCCHHHCCCEEEEEECCCEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
YFRAEAKTLEAASNKENIEIMYNWDLVDVLGGEFVEAARIKNTKTGEEKEIAINGVFGYI
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCHHHHHHHCCCCCCCCCCEEEEEEEEEEC
GTEPKTSMFREYINVKENGYIEGDENMRTNVKGVYVAGDVREKMFRQITTAVSDGTIAAL
CCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEH
HAEKYISEIKER
HHHHHHHHHHCC
>Mature Secondary Structure
MDKEIKELDLIIIGAGPAGLTSAIYASRAKLSTLVLEDNLVGGQVRSTYTVENYPGFTEI
CCCCCCEEEEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEECCCCCCEEE
TGNDLADRIQAQAEACGAIIDEFDFIENVSLKDDEKIIETGDYIYKPKAVIIATGATPKK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCEEEEECCCCCCC
LPIPSESKYLGKGVHYCAVCDGAVYQDEVVAVVGGGNAALEEALYLSNIVKKVIVIRRYD
CCCCCCCCHHHCCCEEEEEECCCEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
YFRAEAKTLEAASNKENIEIMYNWDLVDVLGGEFVEAARIKNTKTGEEKEIAINGVFGYI
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCHHHHHHHCCCCCCCCCCEEEEEEEEEEC
GTEPKTSMFREYINVKENGYIEGDENMRTNVKGVYVAGDVREKMFRQITTAVSDGTIAAL
CCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEH
HAEKYISEIKER
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: thioredoxin; NADP+

Specific reaction: thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1637309 [H]